Restriction map for
pFN21AB5335
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BbeI BbrPI BcgI BfaI BfrI
BglI BglII BlnI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BsrBI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I CfoI ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MluI MroI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AfaI 75 GTAC GT^AC
AlwNI 1065 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 363 GGGCCC GGGCC^C
Asp700I 741 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 229 GAGCTC G[AT]GC[AT]^C
AspI 1875 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1746 TGGCCA TGG^CCA
BamHI 357 GGATCC G^GATCC
BanI 445 GGTGCC G^G[CT][GA]CC
BbvCI 1857 CCTCAGC CCTCAGC(-5/-2)
BciVI 698 GGATAC GTATCC(6/5)
BclI 417 TGATCA T^GATCA
BlpI 51 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 51 GCTGAGC GC^T[ACGT]AGC
BseRI 1045 GAGGAG GAGGAG(10/8)
BsiHKAI 229 GAGCTC G[AT]GC[AT]^C
BsmFI 371 GGGAC GGGAC(10/14)
BsmI 4 GCATTC GAATGC(1/-1)
Bsp1407I 74 TGTACA T^GTACA
BspMI 680 ACCTGC ACCTGC(4/8)
BsrGI 74 TGTACA T^GTACA
BstAPI 285 GCAGCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 51 GCTGAGC GC^T[ACGT]AGC
DraII 429 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 1475 CCATGG C^C[GA][CT]GG
EcoO109I 429 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1260 GAATTC G^AATTC
FbaI 417 TGATCA T^GATCA
MfeI 803 CAATTG C^AATTG
MluNI 1746 TGGCCA TGG^CCA
MscI 1746 TGGCCA TGG^CCA
MseI 833 TTAA T^TAA
MunI 803 CAATTG C^AATTG
NcoI 1475 CCATGG C^CATGG
NdeI 1811 CATATG CA^TATG
PaeR7I 1629 CTCGAG C^TCGAG
PflFI 1875 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 429 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 75 GTAC GT^AC
SacI 229 GAGCTC GAGCT^C
SapI 759 GAAGAGC GCTCTTC(1/4)
SexAI 107 ACCTGGT A^CC[AT]GGT
SfcI 99 CTACAG C^T[GA][CT]AG
SspBI 74 TGTACA T^GTACA
TfiI 1942 GATTC G^A[AT]TC
Tru9I 833 TTAA T^TAA
Tth111I 1875 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1629 CTCGAG C^TCGAG
XmnI 741 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 433 CCTGAGG CC^T[ACGT]AGG
583 CCTGAGG
AvaI 789 CTCGGG C^[CT]CG[GA]G
1629 CTCGAG
BbsI 1050 GAAGAC GAAGAC(2/6)
1872 GAAGAC
BpuAI 1050 GAAGAC GAAGAC(2/6)
1872 GAAGAC
BsaAI 721 TACGTG [CT]AC^GT[GA]
973 TACGTG
BsaWI 836 ACCGGA [AT]^CCGG[AT]
874 TCCGGT
BsoBI 789 CTCGGG C^[CT]CG[GA]G
1629 CTCGAG
BspLU11I 890 ACATGT A^CATGT
1646 ACATGT
BsrFI 201 ACCGGC [GA]^CCGG[CT]
1600 GCCGGT
Bsu36I 433 CCTGAGG CC^T[ACGT]AGG
583 CCTGAGG
Cfr10I 201 ACCGGC [GA]^CCGG[CT]
1600 GCCGGT
EaeI 1410 TGGCCG [CT]^GGCC[GA]
1746 TGGCCA
Eco81I 433 CCTGAGG CC^T[ACGT]AGG
583 CCTGAGG
EcoT14I 1475 CCATGG C^C[AT][AT]GG
1749 CCAAGG
PleI 69 GACTC GAGTC(4/5)
480 GACTC
PvuII 154 CAGCTG CAG^CTG
1013 CAGCTG
SfaNI 87 GATGC GCATC(5/9)
1163 GATGC
StyI 1475 CCATGG C^C[AT][AT]GG
1749 CCAAGG
TaqI 239 TCGA T^CGA
1630 TCGA
TthHB8I 239 TCGA T^CGA
1630 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AflIII AluI AlwI
ApoI AvaII BanII BbvI BcnI
BmyI BpmI BsaI BsaJI BsgI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI Sau3AI
Sau96I ScrFI SmlI TseI Tsp45I
Tsp509I TspRI XhoII