(Circular) (Six-base) MAPSORT of: pFN21AB5335.seq Check: 2218 from: 1 to: 6664 pFN21AB5335 6664 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4474 278 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4007 1937 Size: 2070 4594 Acc65I G'GTAC_C Cuts at: 3985 3985 Size: 6664 AclI AA'CG_TT Cuts at: 1563 4791 5164 1563 Size: 3228 373 3063 Fragments arranged by size: 3228 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2653 2737 2905 2989 3073 3157 3241 3409 Size: 1021 84 168 84 84 84 84 168 Cuts at: 3409 3493 3829 4727 6111 6379 1632 Size: 84 336 898 1384 268 1917 Fragments arranged by size: 1917 1384 1021 898 336 268 168 168 84 84 84 84 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6467 AflIII A'CryG_T Cuts at: 1203 2727 2895 2979 3651 3830 5563 6540 1203 Size: 1524 168 84 672 179 1733 977 1327 Fragments arranged by size: 1733 1524 1327 977 672 179 168 84 AgeI A'CCGG_T Cuts at: 5495 5495 Size: 6664 AhdI GACnn_n'nnGTC Cuts at: 5393 5393 Size: 6664 AleI CACnn'nnGTG Cuts at: 1166 2880 1166 Size: 1714 4950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6664 AlwNI CAG_nnn'CTG Cuts at: 3075 4407 5979 3075 Size: 1332 1572 3760 Fragments arranged by size: 3760 1572 1332 ApaI G_GGCC'C Cuts at: 2372 2372 Size: 6664 ApaLI G'TGCA_C Cuts at: 4721 5877 4721 Size: 1156 5508 ApoI r'AATT_y Cuts at: 1493 2127 2279 3265 3973 4199 4351 6460 1493 Size: 634 152 986 708 226 152 2109 1697 Fragments arranged by size: 2109 1697 986 708 634 226 152 152 AseI AT'TA_AT Cuts at: 160 1032 5218 160 Size: 872 4186 1606 Fragments arranged by size: 4186 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 AvaI C'yCGr_G Cuts at: 1870 1943 2794 3634 3989 1870 Size: 73 851 840 355 4545 Fragments arranged by size: 4545 851 840 355 73 BamHI G'GATC_C Cuts at: 2362 3994 4361 2362 Size: 1632 367 4665 Fragments arranged by size: 4665 1632 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2450 3985 5440 618 Size: 325 217 627 663 1535 1455 1842 Fragments arranged by size: 1842 1535 1455 663 627 325 217 BanII G_rGCy'C Cuts at: 1391 2063 2238 2372 3715 3983 1391 Size: 672 175 134 1343 268 4072 Fragments arranged by size: 4072 1343 672 268 175 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3062 3884 928 Size: 971 1163 822 3708 Fragments arranged by size: 3708 1163 971 822 BbvCI CC'TCA_GC Cuts at: 3863 3863 Size: 6664 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4855 4889 1903 Size: 34 2918 34 3678 Fragments arranged by size: 3678 2918 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2697 4556 5766 2697 Size: 1859 1210 3595 Fragments arranged by size: 3595 1859 1210 BclI T'GATC_A Cuts at: 1548 2422 1548 Size: 874 5790 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5275 136 Size: 107 122 71 631 605 3603 1525 Fragments arranged by size: 3603 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6659 6659 Size: 6664 BlpI GC'TnA_GC Cuts at: 2057 4081 2057 Size: 2024 4640 Bme1580I G_kGCm'C Cuts at: 1790 2372 3252 4725 5881 1790 Size: 582 880 1473 1156 2573 Fragments arranged by size: 2573 1473 1156 880 582 BmgBI CAC'GTC Cuts at: 1318 3831 3885 1318 Size: 2513 54 4097 Fragments arranged by size: 4097 2513 54 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2132 3309 3680 Size: 416 53 83 399 24 715 1177 371 Cuts at: 3680 4395 5353 442 Size: 715 958 1753 Fragments arranged by size: 1753 1177 958 715 715 416 399 371 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6664 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2082 2082 Size: 6664 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2183 2203 2254 2437 2485 5324 1239 Size: 878 66 20 51 183 48 2839 2579 Fragments arranged by size: 2839 2579 878 183 66 51 48 20 Bpu10I CC'TnA_GC Cuts at: 3863 3921 3863 Size: 58 6606 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3332 3836 4789 5654 5952 3332 Size: 504 953 865 298 4044 Fragments arranged by size: 4044 953 865 504 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2122 2171 2404 3614 5327 882 Size: 617 623 49 233 1210 1713 2219 Fragments arranged by size: 2219 1713 1210 623 617 233 49 BsaAI yAC'GTr Cuts at: 493 1204 2728 2980 4455 493 Size: 711 1524 252 1475 2702 Fragments arranged by size: 2702 1524 1475 711 252 BsaBI GATnn'nnATC Cuts at: 6658 6658 Size: 6664 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4471 4853 5488 275 Size: 53 83 186 936 2938 382 635 1451 Fragments arranged by size: 2938 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2841 2879 5097 5495 5769 5916 6327 2841 Size: 38 2218 398 274 147 411 3178 Fragments arranged by size: 3178 2218 411 398 274 147 38 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3095 3125 3296 3326 3548 Size: 30 165 30 1692 30 171 30 222 Cuts at: 3548 3578 1178 Size: 30 4264 Fragments arranged by size: 4264 1692 222 171 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3065 1186 Size: 1879 4785 BseYI C'CCAG_C Cuts at: 2157 2569 5867 6363 2157 Size: 412 3298 496 2458 Fragments arranged by size: 3298 2458 496 412 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2358 2836 2920 3088 3676 3760 3929 Size: 573 622 478 84 168 588 84 169 Cuts at: 3929 3951 1163 Size: 22 3876 Fragments arranged by size: 3876 622 588 573 478 169 168 84 84 22 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4114 4875 5903 1540 Size: 275 186 2113 761 1028 2301 Fragments arranged by size: 2301 2113 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2238 4725 4810 5881 2238 Size: 2487 85 1071 3021 Fragments arranged by size: 3021 2487 1071 85 BsmI GAATG_Cn' Cuts at: 2009 4175 4268 2009 Size: 2166 93 4405 Fragments arranged by size: 4405 2166 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2238 2372 3252 3715 3983 4725 Size: 399 273 175 134 880 463 268 742 Cuts at: 4725 4810 5881 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 880 742 463 399 273 268 175 134 85 BspEI T'CCGG_A Cuts at: 6327 6327 Size: 6664 BspHI T'CATG_A Cuts at: 4553 6283 4553 Size: 1730 4934 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2694 4019 844 Size: 1850 1325 3489 Fragments arranged by size: 3489 1850 1325 BsrBI CCG'CTC Cuts at: 1394 1823 4551 1394 Size: 429 2728 3507 Fragments arranged by size: 3507 2728 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5159 5333 65 Size: 1183 3911 174 1396 Fragments arranged by size: 3911 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2206 3605 5308 5495 1700 Size: 110 396 1399 1703 187 2869 Fragments arranged by size: 2869 1703 1399 396 187 110 BsrGI T'GTAC_A Cuts at: 96 2079 96 Size: 1983 4681 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6664 BssSI C'ACGA_G Cuts at: 4724 5736 4724 Size: 1012 5652 BstAPI GCAn_nnn'nTGC Cuts at: 794 2296 794 Size: 1502 5162 BstBI TT'CG_AA Cuts at: 5468 5468 Size: 6664 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6664 BstYI r'GATC_y Cuts at: 1972 2221 2319 2362 3994 4361 4746 4763 6215 Size: 249 98 43 1632 367 385 17 1452 Cuts at: 6215 6301 6313 6659 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1632 1452 385 367 346 249 98 86 43 17 12 Bsu36I CC'TnA_GG Cuts at: 2439 2589 2439 Size: 150 6514 BtgI C'CryG_G Cuts at: 513 1065 2004 3480 513 Size: 552 939 1476 3697 Fragments arranged by size: 3697 1476 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3181 487 Size: 683 56 1955 3970 Fragments arranged by size: 3970 1955 683 56 BtsI GCAGTG_nn' Cuts at: 2908 3076 3668 4180 4974 4994 2908 Size: 168 592 512 794 20 4578 Fragments arranged by size: 4578 794 592 512 168 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6664 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3142 3177 3394 3429 3814 3849 529 Size: 35 2578 35 217 35 385 35 3344 Fragments arranged by size: 3344 2578 385 217 35 35 35 35 DraI TTT'AAA Cuts at: 3968 3968 Size: 6664 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5671 809 Size: 507 4355 1802 Fragments arranged by size: 4355 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3415 3751 4111 Size: 54 1425 50 275 18 1585 336 360 Cuts at: 4111 5000 6629 8 Size: 889 1629 43 Fragments arranged by size: 1629 1585 1425 889 360 336 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4111 1537 Size: 275 2299 4090 Fragments arranged by size: 4090 2299 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2606 2759 3717 4594 1353 Size: 1253 153 958 877 3423 Fragments arranged by size: 3423 1253 958 877 153 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3304 5251 5625 5771 387 Size: 1064 138 1715 1947 374 146 1280 Fragments arranged by size: 1947 1715 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2236 2236 Size: 6664 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2183 2203 2254 2437 2485 2653 Size: 393 485 66 20 51 183 48 168 Cuts at: 2653 2737 2905 2989 3073 3157 3241 3409 3493 Size: 84 168 84 84 84 84 168 84 Cuts at: 3493 3829 4727 5324 6111 6379 1239 Size: 336 898 597 787 268 1524 Fragments arranged by size: 1524 898 787 597 485 393 336 268 183 168 168 168 84 84 84 84 84 84 66 51 48 20 EcoO109I rG'GnC_Cy Cuts at: 2435 2435 Size: 6664 EcoRI G'AATT_C Cuts at: 3265 3973 4351 3265 Size: 708 378 5578 Fragments arranged by size: 5578 708 378 FspI TGC'GCA Cuts at: 5170 5170 Size: 6664 HaeII r_GCGC'y Cuts at: 1678 5811 1678 Size: 4133 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5305 5337 5379 5411 1373 Size: 32 135 32 3733 32 42 32 2626 Fragments arranged by size: 3733 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2644 2850 3196 4008 4254 669 Size: 988 281 706 206 346 812 246 3079 Fragments arranged by size: 3079 988 812 706 346 281 246 206 HindIII A'AGCT_T Cuts at: 748 748 Size: 6664 HpaI GTT'AAC Cuts at: 4254 4254 Size: 6664 KpnI G_GTAC'C Cuts at: 3989 3989 Size: 6664 MfeI C'AATT_G Cuts at: 2808 4263 2808 Size: 1455 5209 MluI A'CGCG_T Cuts at: 6540 6540 Size: 6664 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3462 5778 5962 1305 Size: 2157 2316 184 2007 Fragments arranged by size: 2316 2157 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3753 10 Size: 54 1425 343 1921 2921 Fragments arranged by size: 2921 1921 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2332 2880 3820 4622 4981 5140 518 Size: 648 1166 548 940 802 359 159 2042 Fragments arranged by size: 2042 1166 940 802 648 548 359 159 MspA1I CmG'CkG Cuts at: 1606 2161 2701 3020 4080 4757 5905 6150 1606 Size: 555 540 319 1060 677 1148 245 2120 Fragments arranged by size: 2120 1148 1060 677 555 540 319 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3480 513 Size: 552 939 1476 3697 Fragments arranged by size: 3697 1476 939 552 NdeI CA'TA_TG Cuts at: 387 3817 387 Size: 3430 3234 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6664 NotI GC'GGCC_GC Cuts at: 4111 4111 Size: 6664 NspI r_CATG'y Cuts at: 1132 1229 2297 2331 2899 3655 4022 5567 1132 Size: 97 1068 34 568 756 367 1545 2229 Fragments arranged by size: 2229 1545 1068 756 568 367 97 34 PasI CC'CwG_GG Cuts at: 1348 2372 1348 Size: 1024 5640 PciI A'CATG_T Cuts at: 2895 3651 5563 2895 Size: 756 1912 3996 Fragments arranged by size: 3996 1912 756 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6664 PmeI GTTT'AAAC Cuts at: 3968 3968 Size: 6664 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3365 3397 4733 4765 6246 6278 3365 Size: 32 1336 32 1481 32 3751 Fragments arranged by size: 3751 1481 1336 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2435 2435 Size: 6664 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6664 PsiI TTA'TAA Cuts at: 4234 4234 Size: 6664 PspOMI G'GGCC_C Cuts at: 2368 2368 Size: 6664 PspXI vC'TCGA_Gb Cuts at: 3634 3634 Size: 6664 PstI C_TGCA'G Cuts at: 830 4016 830 Size: 3186 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6664 PvuII CAG'CTG Cuts at: 2161 3020 2161 Size: 859 5805 SacI G_AGCT'C Cuts at: 2238 2238 Size: 6664 SalI G'TCGA_C Cuts at: 1936 4006 1936 Size: 2070 4594 SapI GCTCTTCn'nnn_ Cuts at: 2759 2759 Size: 6664 SbfI CC_TGCA'GG Cuts at: 4016 4016 Size: 6664 ScaI AGT'ACT Cuts at: 4912 6624 4912 Size: 1712 4952 SexAI A'CCwGG_T Cuts at: 2112 2112 Size: 6664 SfcI C'TryA_G Cuts at: 826 1045 2104 4012 5147 5828 6019 826 Size: 219 1059 1908 1135 681 191 1471 Fragments arranged by size: 1908 1471 1135 1059 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6664 SmaI CCC'GGG Cuts at: 1872 3991 1872 Size: 2119 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3347 3634 3851 4768 5669 5931 Size: 197 926 1404 287 217 917 901 262 Cuts at: 5931 820 Size: 1553 Fragments arranged by size: 1553 1404 926 917 901 287 262 217 197 SnaBI TAC'GTA Cuts at: 493 4455 493 Size: 3962 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6664 SphI G_CATG'C Cuts at: 1132 4022 1132 Size: 2890 3774 SspI AAT'ATT Cuts at: 5 52 4588 5 Size: 47 4536 2081 Fragments arranged by size: 4536 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6664 StyI C'CwwG_G Cuts at: 513 1065 2004 3480 3754 4103 513 Size: 552 939 1476 274 349 3074 Fragments arranged by size: 3074 1476 939 552 349 274 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2051 2171 4691 4708 4861 5046 5491 Size: 1255 471 120 2520 17 153 185 445 Cuts at: 5491 325 Size: 1498 Fragments arranged by size: 2520 1498 1255 471 445 185 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2079 4910 6622 Size: 274 80 33 51 1443 102 2831 1712 Cuts at: 6622 96 Size: 138 Fragments arranged by size: 2831 1712 1443 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6624 6656 6624 Size: 32 6632 Tth111I GACn'n_nGTC Cuts at: 1316 3883 1316 Size: 2567 4097 XbaI T'CTAG_A Cuts at: 4000 4000 Size: 6664 XhoI C'TCGA_G Cuts at: 1943 3634 1943 Size: 1691 4973 XmaI C'CCGG_G Cuts at: 1870 3989 1870 Size: 2119 4545 XmnI GAAnn'nnTTC Cuts at: 2750 4793 2750 Size: 2043 4621 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4472 276 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI