(Circular) (Six-base) MAPSORT of: pFN21AB5334.seq Check: 1108 from: 1 to: 6271 pFN21AB5334 6271 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4081 278 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3614 1937 Size: 1677 4594 Acc65I G'GTAC_C Cuts at: 3592 3592 Size: 6271 AclI AA'CG_TT Cuts at: 1563 4398 4771 1563 Size: 2835 373 3063 Fragments arranged by size: 3063 2835 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2891 4334 5718 5986 1632 Size: 416 843 1443 1384 268 1917 Fragments arranged by size: 1917 1443 1384 843 416 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6074 AflIII A'CryG_T Cuts at: 1203 5170 6147 1203 Size: 3967 977 1327 Fragments arranged by size: 3967 1327 977 AgeI A'CCGG_T Cuts at: 5102 5102 Size: 6271 AhdI GACnn_n'nnGTC Cuts at: 5000 5000 Size: 6271 AleI CACnn'nnGTG Cuts at: 1166 2759 3115 1166 Size: 1593 356 4322 Fragments arranged by size: 4322 1593 356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6271 AlwNI CAG_nnn'CTG Cuts at: 2104 4014 5586 2104 Size: 1910 1572 2789 Fragments arranged by size: 2789 1910 1572 ApaLI G'TGCA_C Cuts at: 2110 4328 5484 2110 Size: 2218 1156 2897 Fragments arranged by size: 2897 2218 1156 ApoI r'AATT_y Cuts at: 1493 2437 3580 3806 3958 6067 1493 Size: 944 1143 226 152 2109 1697 Fragments arranged by size: 2109 1697 1143 944 226 152 AseI AT'TA_AT Cuts at: 160 1032 4825 160 Size: 872 3793 1606 Fragments arranged by size: 3793 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 AvaI C'yCGr_G Cuts at: 1870 1943 2054 3596 1870 Size: 73 111 1542 4545 Fragments arranged by size: 4545 1542 111 73 AvrII C'CTAG_G Cuts at: 3296 3296 Size: 6271 BamHI G'GATC_C Cuts at: 2164 3601 3968 2164 Size: 1437 367 4467 Fragments arranged by size: 4467 1437 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2050 2651 3592 5047 618 Size: 325 217 627 263 601 941 1455 1842 Fragments arranged by size: 1842 1455 941 627 601 325 263 217 BanII G_rGCy'C Cuts at: 1391 3590 1391 Size: 2199 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5300 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4462 4496 1903 Size: 34 2525 34 3678 Fragments arranged by size: 3678 2525 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2955 4163 5373 2955 Size: 1208 1210 3853 Fragments arranged by size: 3853 1210 1208 BclI T'GATC_A Cuts at: 1548 1548 Size: 6271 BfrBI ATG'CAT Cuts at: 2722 2722 Size: 6271 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4882 136 Size: 107 122 71 631 605 3210 1525 Fragments arranged by size: 3210 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2902 6266 2902 Size: 3364 2907 BlpI GC'TnA_GC Cuts at: 3688 3688 Size: 6271 Bme1580I G_kGCm'C Cuts at: 1790 2055 2114 2486 2654 4332 5488 1790 Size: 265 59 372 168 1678 1156 2573 Fragments arranged by size: 2573 1678 1156 372 265 168 59 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6271 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2386 4002 4960 Size: 416 53 83 399 24 969 1616 958 Cuts at: 4960 442 Size: 1753 Fragments arranged by size: 1753 1616 969 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6271 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2332 2758 2332 Size: 426 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2196 2282 3309 4931 1239 Size: 827 130 86 1027 1622 2579 Fragments arranged by size: 2579 1622 1027 827 130 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4396 5261 5559 4396 Size: 865 298 5108 Fragments arranged by size: 5108 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2180 2549 3372 3433 4934 882 Size: 617 681 369 823 61 1501 2219 Fragments arranged by size: 2219 1501 823 681 617 369 61 BsaAI yAC'GTr Cuts at: 493 1204 4062 493 Size: 711 2858 2702 Fragments arranged by size: 2858 2702 711 BsaBI GATnn'nnATC Cuts at: 6265 6265 Size: 6271 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3416 4078 4460 5095 Size: 53 83 186 936 1883 662 382 635 Cuts at: 5095 275 Size: 1451 Fragments arranged by size: 1883 1451 936 662 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4704 5102 5376 5523 5934 4704 Size: 398 274 147 411 5041 Fragments arranged by size: 5041 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6046 Fragments arranged by size: 6046 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6271 BseYI C'CCAG_C Cuts at: 2071 3419 3530 5474 5970 2071 Size: 1348 111 1944 496 2372 Fragments arranged by size: 2372 1944 1348 496 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5698 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3721 4482 5510 1540 Size: 275 186 1720 761 1028 2301 Fragments arranged by size: 2301 1720 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2114 4332 4417 5488 2114 Size: 2218 85 1071 2897 Fragments arranged by size: 2897 2218 1071 85 BsmI GAATG_Cn' Cuts at: 2287 2545 3782 3875 2287 Size: 258 1237 93 4683 Fragments arranged by size: 4683 1237 258 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2051 3407 2051 Size: 1356 4915 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2114 2486 2654 3590 4332 4417 Size: 399 265 59 372 168 936 742 85 Cuts at: 4417 5488 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 936 742 399 372 265 168 85 59 BspEI T'CCGG_A Cuts at: 5934 5934 Size: 6271 BspHI T'CATG_A Cuts at: 4160 5890 4160 Size: 1730 4541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3626 844 Size: 2782 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2697 4158 1394 Size: 429 874 1461 3507 Fragments arranged by size: 3507 1461 874 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4766 4940 65 Size: 1183 3518 174 1396 Fragments arranged by size: 3518 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3555 4915 5102 1700 Size: 110 1745 1360 187 2869 Fragments arranged by size: 2869 1745 1360 187 110 BsrGI T'GTAC_A Cuts at: 96 3189 96 Size: 3093 3178 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6271 BssSI C'ACGA_G Cuts at: 4331 5343 4331 Size: 1012 5259 BstAPI GCAn_nnn'nTGC Cuts at: 794 2104 2719 794 Size: 1310 615 4346 Fragments arranged by size: 4346 1310 615 BstBI TT'CG_AA Cuts at: 5075 5075 Size: 6271 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6271 BstXI CCAn_nnnn'nTGG Cuts at: 3117 3117 Size: 6271 BstYI r'GATC_y Cuts at: 1972 2164 2902 3030 3601 3968 4353 4370 5822 Size: 192 738 128 571 367 385 17 1452 Cuts at: 5822 5908 5920 6266 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 738 571 385 367 346 192 128 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2093 2646 2093 Size: 553 5718 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5719 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5532 Fragments arranged by size: 5532 683 56 BtsI GCAGTG_nn' Cuts at: 3787 4581 4601 3787 Size: 794 20 5457 Fragments arranged by size: 5457 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6236 DraI TTT'AAA Cuts at: 2929 3575 2929 Size: 646 5625 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3155 5278 809 Size: 507 1839 2123 1802 Fragments arranged by size: 2123 1839 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2039 2322 3542 Size: 54 1425 50 275 18 209 283 1220 Cuts at: 3542 3553 3718 4607 6236 8 Size: 11 165 889 1629 43 Fragments arranged by size: 1629 1425 1220 889 283 275 209 165 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 3718 1537 Size: 275 1906 4090 Fragments arranged by size: 4090 1906 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2178 3191 4201 1353 Size: 825 1013 1010 3423 Fragments arranged by size: 3423 1013 1010 825 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4858 5232 5378 387 Size: 1064 138 3269 374 146 1280 Fragments arranged by size: 3269 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2066 2196 2282 2891 3309 4334 Size: 393 416 18 130 86 609 418 1025 Cuts at: 4334 4931 5718 5986 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1025 787 609 597 418 416 393 268 130 86 18 EcoO109I rG'GnC_Cy Cuts at: 2426 3473 2426 Size: 1047 5224 EcoRI G'AATT_C Cuts at: 3580 3958 3580 Size: 378 5893 FspI TGC'GCA Cuts at: 2469 4777 2469 Size: 2308 3963 FspAI rTGC'GCAy Cuts at: 2469 2469 Size: 6271 HaeII r_GCGC'y Cuts at: 1678 2258 5418 1678 Size: 580 3160 2531 Fragments arranged by size: 3160 2531 580 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2548 2580 3369 3401 4912 Size: 32 135 32 976 32 789 32 1511 Cuts at: 4912 4944 4986 5018 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1511 976 789 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3258 3615 3861 669 Size: 988 281 1320 357 246 3079 Fragments arranged by size: 3079 1320 988 357 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6271 HpaI GTT'AAC Cuts at: 3861 3861 Size: 6271 KpnI G_GTAC'C Cuts at: 3596 3596 Size: 6271 MfeI C'AATT_G Cuts at: 3870 3870 Size: 6271 MluI A'CGCG_T Cuts at: 6147 6147 Size: 6271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2607 3061 3355 5385 5569 1305 Size: 1302 454 294 2030 184 2007 Fragments arranged by size: 2030 2007 1302 454 294 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2324 10 Size: 54 1425 343 492 3957 Fragments arranged by size: 3957 1425 492 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2759 3115 4229 4588 4747 518 Size: 648 1593 356 1114 359 159 2042 Fragments arranged by size: 2042 1593 1114 648 359 356 159 MspA1I CmG'CkG Cuts at: 1606 2044 2573 2839 2938 3687 4364 5512 5757 Size: 438 529 266 99 749 677 1148 245 Cuts at: 5757 1606 Size: 2120 Fragments arranged by size: 2120 1148 749 677 529 438 266 245 99 NaeI GCC'GGC Cuts at: 3557 3557 Size: 6271 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5719 NdeI CA'TA_TG Cuts at: 387 2200 2472 387 Size: 1813 272 4186 Fragments arranged by size: 4186 1813 272 NgoMIV G'CCGG_C Cuts at: 3555 3555 Size: 6271 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6271 NotI GC'GGCC_GC Cuts at: 3718 3718 Size: 6271 NsiI A_TGCA'T Cuts at: 2724 2724 Size: 6271 NspI r_CATG'y Cuts at: 1132 1229 2722 3629 5174 1132 Size: 97 1493 907 1545 2229 Fragments arranged by size: 2229 1545 1493 907 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6271 PciI A'CATG_T Cuts at: 5170 5170 Size: 6271 PflMI CCAn_nnn'nTGG Cuts at: 1108 3551 1108 Size: 2443 3828 PfoI T'CCnGG_A Cuts at: 3325 3469 3325 Size: 144 6127 PmeI GTTT'AAAC Cuts at: 3575 3575 Size: 6271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2496 2528 4340 4372 5853 5885 2496 Size: 32 1812 32 1481 32 2882 Fragments arranged by size: 2882 1812 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3473 3473 Size: 6271 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6271 PsiI TTA'TAA Cuts at: 3841 3841 Size: 6271 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3009 3041 3009 Size: 32 6239 PstI C_TGCA'G Cuts at: 830 2715 3623 830 Size: 1885 908 3478 Fragments arranged by size: 3478 1885 908 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6271 PvuII CAG'CTG Cuts at: 2573 2839 2938 2573 Size: 266 99 5906 Fragments arranged by size: 5906 266 99 SalI G'TCGA_C Cuts at: 1936 3613 1936 Size: 1677 4594 SbfI CC_TGCA'GG Cuts at: 3623 3623 Size: 6271 ScaI AGT'ACT Cuts at: 4519 6231 4519 Size: 1712 4559 SfcI C'TryA_G Cuts at: 826 1045 2515 2711 3619 4754 5435 5626 826 Size: 219 1470 196 908 1135 681 191 1471 Fragments arranged by size: 1471 1470 1135 908 681 219 196 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6271 SmaI CCC'GGG Cuts at: 1872 3598 1872 Size: 1726 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4375 5276 5538 820 Size: 197 926 2432 901 262 1553 Fragments arranged by size: 2432 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4062 493 Size: 3569 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6271 SphI G_CATG'C Cuts at: 1132 3629 1132 Size: 2497 3774 SspI AAT'ATT Cuts at: 5 52 4195 5 Size: 47 4143 2081 Fragments arranged by size: 4143 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6271 StyI C'CwwG_G Cuts at: 513 1065 2190 2377 3296 3710 513 Size: 552 1125 187 919 414 3074 Fragments arranged by size: 3074 1125 919 552 414 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4298 4315 4468 4653 5098 325 Size: 1255 2718 17 153 185 445 1498 Fragments arranged by size: 2718 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2715 3189 4517 Size: 274 80 33 51 1443 738 474 1328 Cuts at: 4517 6229 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1328 738 474 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6231 6263 6231 Size: 32 6239 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6271 XbaI T'CTAG_A Cuts at: 3607 3607 Size: 6271 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6271 XmaI C'CCGG_G Cuts at: 1870 3596 1870 Size: 1726 4545 XmnI GAAnn'nnTTC Cuts at: 4400 4400 Size: 6271 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4079 276 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI KasI NarI NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI