(Circular) (Six-base) MAPSORT of: pFN21AB5299.seq Check: 274 from: 1 to: 6061 pFN21AB5299 6061 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2172 2172 Size: 6061 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3019 3404 1937 Size: 1082 385 4594 Fragments arranged by size: 4594 1082 385 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2339 2387 3000 4124 5508 5776 1632 Size: 707 48 613 1124 1384 268 1917 Fragments arranged by size: 1917 1384 1124 707 613 268 48 AfeI AGC'GCT Cuts at: 2124 2124 Size: 6061 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 2794 4960 5937 1203 Size: 1591 2166 977 1327 Fragments arranged by size: 2166 1591 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 2795 1166 Size: 1629 4432 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 3328 3804 5376 3328 Size: 476 1572 4013 Fragments arranged by size: 4013 1572 476 ApaLI G'TGCA_C Cuts at: 2510 4118 5274 2510 Size: 1608 1156 3297 Fragments arranged by size: 3297 1608 1156 ApoI r'AATT_y Cuts at: 1493 2046 3370 3596 3748 5857 1493 Size: 553 1324 226 152 2109 1697 Fragments arranged by size: 2109 1697 1324 553 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 2118 2591 3386 1870 Size: 73 175 473 795 4545 Fragments arranged by size: 4545 795 473 175 73 BamHI G'GATC_C Cuts at: 3118 3391 3758 3118 Size: 273 367 5421 Fragments arranged by size: 5421 367 273 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3382 4837 618 Size: 325 217 627 1595 1455 1842 Fragments arranged by size: 1842 1595 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2072 2659 3196 3380 1391 Size: 681 587 537 184 4072 Fragments arranged by size: 4072 681 587 537 184 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2145 2940 928 Size: 971 246 795 4049 Fragments arranged by size: 4049 971 795 246 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2134 2168 4252 4286 1903 Size: 34 197 34 2084 34 3678 Fragments arranged by size: 3678 2084 197 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4672 136 Size: 107 122 71 631 605 3000 1525 Fragments arranged by size: 3000 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 2514 4122 5278 1790 Size: 724 1608 1156 2573 Fragments arranged by size: 2573 1608 1156 724 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2066 2200 3792 Size: 416 53 83 399 24 649 134 1592 Cuts at: 3792 4750 442 Size: 958 1753 Fragments arranged by size: 1753 1592 958 649 416 399 134 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2158 2397 2158 Size: 239 5822 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2046 2206 2533 3326 4721 1239 Size: 807 160 327 793 1395 2579 Fragments arranged by size: 2579 1395 807 793 327 160 Bpu10I CC'TnA_GC Cuts at: 2126 2658 2126 Size: 532 5529 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2198 4186 5051 5349 2198 Size: 1988 865 298 2910 Fragments arranged by size: 2910 1988 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2968 4724 882 Size: 617 1469 1756 2219 Fragments arranged by size: 2219 1756 1469 617 BsaAI yAC'GTr Cuts at: 493 1204 3852 493 Size: 711 2648 2702 Fragments arranged by size: 2702 2648 711 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2096 3868 4250 4885 Size: 53 83 186 936 563 1772 382 635 Cuts at: 4885 275 Size: 1451 Fragments arranged by size: 1772 1451 936 635 563 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2607 3108 4494 4892 5166 5313 5724 2607 Size: 501 1386 398 274 147 411 2944 Fragments arranged by size: 2944 1386 501 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5836 Fragments arranged by size: 5836 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2313 1186 Size: 1127 4934 BseYI C'CCAG_C Cuts at: 2740 2813 5264 5760 2740 Size: 73 2451 496 3041 Fragments arranged by size: 3041 2451 496 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5488 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4272 5300 1540 Size: 275 186 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2343 2514 2659 2792 2988 3312 4122 4207 5278 Size: 171 145 133 196 324 810 85 1071 Cuts at: 5278 2343 Size: 3126 Fragments arranged by size: 3126 1071 810 324 196 171 145 133 85 BsmI GAATG_Cn' Cuts at: 3362 3572 3665 3362 Size: 210 93 5758 Fragments arranged by size: 5758 210 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2343 2514 2659 2792 2988 3196 Size: 399 282 271 171 145 133 196 208 Cuts at: 3196 3312 3380 4122 4207 5278 1391 Size: 116 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 282 271 208 196 171 145 133 116 85 68 BspEI T'CCGG_A Cuts at: 3108 5724 3108 Size: 2616 3445 BspHI T'CATG_A Cuts at: 3282 3950 5680 3282 Size: 668 1730 3663 Fragments arranged by size: 3663 1730 668 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2172 3416 844 Size: 1328 1244 3489 Fragments arranged by size: 3489 1328 1244 BsrBI CCG'CTC Cuts at: 1394 1823 2547 3948 1394 Size: 429 724 1401 3507 Fragments arranged by size: 3507 1401 724 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4556 4730 65 Size: 1183 3308 174 1396 Fragments arranged by size: 3308 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2574 3060 4705 4892 1700 Size: 110 764 486 1645 187 2869 Fragments arranged by size: 2869 1645 764 486 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6061 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 2992 794 Size: 2198 3863 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 2553 1184 Size: 1369 4692 BstXI CCAn_nnnn'nTGG Cuts at: 2184 3130 2184 Size: 946 5115 BstYI r'GATC_y Cuts at: 1972 3118 3391 3758 4143 4160 5612 5698 5710 Size: 1146 273 367 385 17 1452 86 12 Cuts at: 5710 6056 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1146 385 367 346 273 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2499 2721 2856 513 Size: 552 1434 222 135 3718 Fragments arranged by size: 3718 1434 552 222 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 2734 3577 4371 4391 2734 Size: 843 794 20 4404 Fragments arranged by size: 4404 843 794 20 ClaI AT'CG_AT Cuts at: 1554 2317 1554 Size: 763 5298 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DraIII CAC_nnn'GTG Cuts at: 2857 2857 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3289 3508 4397 Size: 54 1425 50 275 18 1459 219 889 Cuts at: 4397 6026 8 Size: 1629 43 Fragments arranged by size: 1629 1459 1425 889 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2976 3991 1353 Size: 1623 1015 3423 Fragments arranged by size: 3423 1623 1015 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2138 2621 4648 5022 5168 387 Size: 1064 138 549 483 2027 374 146 1280 Fragments arranged by size: 2027 1280 1064 549 483 374 146 138 EcoICRI GAG'CTC Cuts at: 2657 2657 Size: 6061 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2206 2339 2387 2533 3000 3326 Size: 393 414 160 133 48 146 467 326 Cuts at: 3326 4124 4721 5508 5776 1239 Size: 798 597 787 268 1524 Fragments arranged by size: 1524 798 787 597 467 414 393 326 268 160 146 133 48 EcoNI CCTnn'n_nnAGG Cuts at: 2328 2328 Size: 6061 EcoO109I rG'GnC_Cy Cuts at: 2484 2663 2484 Size: 179 5882 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 FspI TGC'GCA Cuts at: 2160 4567 2160 Size: 2407 3654 HaeII r_GCGC'y Cuts at: 1678 2126 5208 1678 Size: 448 3082 2531 Fragments arranged by size: 3082 2531 448 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2005 2037 3080 3103 3112 Size: 32 135 32 433 32 1043 23 9 Cuts at: 3112 3135 3170 3202 4702 4734 4776 4808 1373 Size: 23 35 32 1500 32 42 32 2626 Fragments arranged by size: 2626 1500 1043 433 135 42 35 32 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 2247 3660 2247 Size: 1413 4648 MluI A'CGCG_T Cuts at: 2794 5937 2794 Size: 3143 2918 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5175 5359 1305 Size: 3870 184 2007 Fragments arranged by size: 3870 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4239 Fragments arranged by size: 4239 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2331 2795 4019 4378 4537 518 Size: 648 1165 464 1224 359 159 2042 Fragments arranged by size: 2042 1224 1165 648 464 359 159 MspA1I CmG'CkG Cuts at: 1606 2599 2806 3477 4154 5302 5547 1606 Size: 993 207 671 677 1148 245 2120 Fragments arranged by size: 2120 1148 993 677 671 245 207 NcoI C'CATG_G Cuts at: 513 1065 2721 513 Size: 552 1656 3853 Fragments arranged by size: 3853 1656 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 3419 4964 1132 Size: 97 2190 1545 2229 Fragments arranged by size: 2229 2190 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 2959 3328 1108 Size: 1851 369 3841 Fragments arranged by size: 3841 1851 369 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2484 2663 2484 Size: 179 5882 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PspXI vC'TCGA_Gb Cuts at: 2118 2118 Size: 6061 PstI C_TGCA'G Cuts at: 830 3413 830 Size: 2583 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 2806 2806 Size: 6061 SacI G_AGCT'C Cuts at: 2659 2659 Size: 6061 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SanDI GG'GwC_CC Cuts at: 2663 2663 Size: 6061 SapI GCTCTTCn'nnn_ Cuts at: 2976 2976 Size: 6061 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SfcI C'TryA_G Cuts at: 826 1045 3016 3409 4544 5225 5416 826 Size: 219 1971 393 1135 681 191 1471 Fragments arranged by size: 1971 1471 1135 681 393 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2118 2213 4165 5066 5328 820 Size: 197 926 175 95 1952 901 262 1553 Fragments arranged by size: 1952 1553 926 901 262 197 175 95 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3105 3985 5 Size: 47 3053 880 2081 Fragments arranged by size: 3053 2081 880 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 2721 3500 513 Size: 552 1656 779 3074 Fragments arranged by size: 3074 1656 779 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4088 4105 4258 4443 4888 325 Size: 1255 2508 17 153 185 445 1498 Fragments arranged by size: 2508 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4307 6019 96 Size: 274 80 33 51 1443 2330 1712 138 Fragments arranged by size: 2330 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2388 2420 2681 2713 2784 2816 3302 3334 6021 Size: 32 261 32 71 32 486 32 2687 Cuts at: 6021 6053 2388 Size: 32 2396 Fragments arranged by size: 2687 2396 486 261 71 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2497 2497 Size: 6061 XhoI C'TCGA_G Cuts at: 1943 2118 1943 Size: 175 5886 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 2352 4190 2352 Size: 1838 4223 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI