(Circular) (Six-base) MAPSORT of: pFN21AB5298.seq Check: 4971 from: 1 to: 6607 pFN21AB5298 6607 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2671 3365 2671 Size: 694 5913 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4417 278 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2469 2592 3057 3950 1937 Size: 532 123 465 893 4594 Fragments arranged by size: 4594 893 532 465 123 Acc65I G'GTAC_C Cuts at: 3928 3928 Size: 6607 AclI AA'CG_TT Cuts at: 1563 4734 5107 1563 Size: 3171 373 3063 Fragments arranged by size: 3171 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2212 2378 4670 6054 6322 1632 Size: 580 166 2292 1384 268 1917 Fragments arranged by size: 2292 1917 1384 580 268 166 AfeI AGC'GCT Cuts at: 3257 3257 Size: 6607 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6410 AflIII A'CryG_T Cuts at: 1203 5506 6483 1203 Size: 4303 977 1327 Fragments arranged by size: 4303 1327 977 AgeI A'CCGG_T Cuts at: 5438 5438 Size: 6607 AhdI GACnn_n'nnGTC Cuts at: 5336 5336 Size: 6607 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6607 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3015 3056 3641 1184 Size: 1831 41 585 4150 Fragments arranged by size: 4150 1831 585 41 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2006 2038 2006 Size: 32 6575 AlwNI CAG_nnn'CTG Cuts at: 2110 2773 3157 4350 5922 2110 Size: 663 384 1193 1572 2795 Fragments arranged by size: 2795 1572 1193 663 384 ApaI G_GGCC'C Cuts at: 2656 3735 2656 Size: 1079 5528 ApaLI G'TGCA_C Cuts at: 3536 3692 4664 5820 3536 Size: 156 972 1156 4323 Fragments arranged by size: 4323 1156 972 156 ApoI r'AATT_y Cuts at: 1493 3916 4142 4294 6403 1493 Size: 2423 226 152 2109 1697 Fragments arranged by size: 2423 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 3451 3451 Size: 6607 AseI AT'TA_AT Cuts at: 160 1032 5161 160 Size: 872 4129 1606 Fragments arranged by size: 4129 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 AvaI C'yCGr_G Cuts at: 1870 1943 3932 1870 Size: 73 1989 4545 Fragments arranged by size: 4545 1989 73 BamHI G'GATC_C Cuts at: 3937 4304 3937 Size: 367 6240 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3928 5383 618 Size: 325 217 627 2141 1455 1842 Fragments arranged by size: 2141 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2349 2656 2715 2870 3735 3926 1391 Size: 958 307 59 155 865 191 4072 Fragments arranged by size: 4072 958 865 307 191 155 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5636 BbvCI CC'TCA_GC Cuts at: 2603 2757 2945 2603 Size: 154 188 6265 Fragments arranged by size: 6265 188 154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2167 2201 4798 4832 1903 Size: 34 230 34 2597 34 3678 Fragments arranged by size: 3678 2597 230 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3515 4499 5709 3515 Size: 984 1210 4413 Fragments arranged by size: 4413 1210 984 BclI T'GATC_A Cuts at: 1548 1548 Size: 6607 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3008 3448 3684 Size: 107 122 71 631 605 1336 440 236 Cuts at: 3684 5218 136 Size: 1534 1525 Fragments arranged by size: 1534 1525 1336 631 605 440 236 122 107 71 BglII A'GATC_T Cuts at: 2697 6602 2697 Size: 3905 2702 BlpI GC'TnA_GC Cuts at: 2348 4024 2348 Size: 1676 4931 Bme1580I G_kGCm'C Cuts at: 1790 2656 3540 3606 3696 3735 4668 5824 1790 Size: 866 884 66 90 39 933 1156 2573 Fragments arranged by size: 2573 1156 933 884 866 90 66 39 BmgBI CAC'GTC Cuts at: 1318 3535 3564 1318 Size: 2217 29 4361 Fragments arranged by size: 4361 2217 29 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2987 3886 4338 Size: 416 53 83 399 24 1570 899 452 Cuts at: 4338 5296 442 Size: 958 1753 Fragments arranged by size: 1753 1570 958 899 452 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6607 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 2639 2696 3133 3356 3880 5267 1239 Size: 1124 276 57 437 223 524 1387 2579 Fragments arranged by size: 2579 1387 1124 524 437 276 223 57 Bpu10I CC'TnA_GC Cuts at: 2354 2603 2757 2945 2354 Size: 249 154 188 6016 Fragments arranged by size: 6016 249 188 154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2515 2747 3284 3821 4732 5597 5895 2515 Size: 232 537 537 911 865 298 3227 Fragments arranged by size: 3227 911 865 537 537 298 232 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2627 2629 5270 882 Size: 617 1128 2 2641 2219 Fragments arranged by size: 2641 2219 1128 617 2 BsaAI yAC'GTr Cuts at: 493 1204 3289 3691 4398 493 Size: 711 2085 402 707 2702 Fragments arranged by size: 2702 2085 711 707 402 BsaBI GATnn'nnATC Cuts at: 3350 6601 3350 Size: 3251 3356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4414 4796 5431 275 Size: 53 83 186 936 2881 382 635 1451 Fragments arranged by size: 2881 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2835 5040 5438 5712 5859 6270 2835 Size: 2205 398 274 147 411 3172 Fragments arranged by size: 3172 2205 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2006 2036 2870 2900 1178 Size: 30 165 30 603 30 834 30 4885 Fragments arranged by size: 4885 834 603 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2591 3467 3896 1186 Size: 841 564 876 429 3897 Fragments arranged by size: 3897 876 841 564 429 BseYI C'CCAG_C Cuts at: 2028 2243 2792 2855 3599 3720 3735 5810 6306 Size: 215 549 63 744 121 15 2075 496 Cuts at: 6306 2028 Size: 2329 Fragments arranged by size: 2329 2075 744 549 496 215 121 63 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2031 2690 2705 3544 1163 Size: 573 295 659 15 839 4226 Fragments arranged by size: 4226 839 659 573 295 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2888 3364 4057 4818 5846 1540 Size: 275 186 887 476 693 761 1028 2301 Fragments arranged by size: 2301 1028 887 761 693 476 275 186 BsiHKAI G_wGCw'C Cuts at: 2349 3045 3171 3540 3696 3777 4668 4753 5824 Size: 696 126 369 156 81 891 85 1071 Cuts at: 5824 2349 Size: 3132 Fragments arranged by size: 3132 1071 891 696 369 156 126 85 81 BsmI GAATG_Cn' Cuts at: 2919 3250 4118 4211 2919 Size: 331 868 93 5315 Fragments arranged by size: 5315 868 331 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2349 2656 2715 2870 3045 3171 3540 Size: 399 559 307 59 155 175 126 369 Cuts at: 3540 3606 3696 3735 3777 3926 4668 4753 5824 Size: 66 90 39 42 149 742 85 1071 Cuts at: 5824 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 559 399 369 307 175 155 149 126 90 85 66 59 42 39 BspEI T'CCGG_A Cuts at: 6270 6270 Size: 6607 BspHI T'CATG_A Cuts at: 4496 6226 4496 Size: 1730 4877 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2671 3189 3365 3962 844 Size: 1827 518 176 597 3489 Fragments arranged by size: 3489 1827 597 518 176 BsrBI CCG'CTC Cuts at: 1394 1823 3660 3789 4494 1394 Size: 429 1837 129 705 3507 Fragments arranged by size: 3507 1837 705 429 129 BsrDI GCAATG_nn' Cuts at: 65 1248 2365 3618 5102 5276 65 Size: 1183 1117 1253 1484 174 1396 Fragments arranged by size: 1484 1396 1253 1183 1117 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2472 5251 5438 1700 Size: 110 662 2779 187 2869 Fragments arranged by size: 2869 2779 662 187 110 BsrGI T'GTAC_A Cuts at: 96 2362 96 Size: 2266 4341 BssHII G'CGCG_C Cuts at: 1925 3451 1925 Size: 1526 5081 BssSI C'ACGA_G Cuts at: 2708 4667 5679 2708 Size: 1959 1012 3636 Fragments arranged by size: 3636 1959 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2773 794 Size: 1979 4628 BstBI TT'CG_AA Cuts at: 5411 5411 Size: 6607 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6607 BstYI r'GATC_y Cuts at: 1972 2397 2697 3147 3639 3937 4304 4689 4706 Size: 425 300 450 492 298 367 385 17 Cuts at: 4706 6158 6244 6256 6602 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 492 450 425 385 367 346 300 298 86 17 12 BstZ17I GTA'TAC Cuts at: 3058 3058 Size: 6607 BtgI C'CryG_G Cuts at: 513 1065 2004 2284 3125 3670 3811 513 Size: 552 939 280 841 545 141 3309 Fragments arranged by size: 3309 939 841 552 545 280 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2180 3019 487 Size: 683 56 954 839 4075 Fragments arranged by size: 4075 954 839 683 56 BtsI GCAGTG_nn' Cuts at: 2065 2997 3394 4123 4917 4937 2065 Size: 932 397 729 794 20 3735 Fragments arranged by size: 3735 932 794 729 397 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6607 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6572 DraI TTT'AAA Cuts at: 3911 3911 Size: 6607 DraIII CAC_nnn'GTG Cuts at: 2071 2290 3544 2071 Size: 219 1254 5134 Fragments arranged by size: 5134 1254 219 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3080 5614 809 Size: 507 1764 2534 1802 Fragments arranged by size: 2534 1802 1764 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2814 3361 3655 Size: 54 1425 50 275 18 984 547 294 Cuts at: 3655 4054 4943 6572 8 Size: 399 889 1629 43 Fragments arranged by size: 1629 1425 984 889 547 399 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3361 4054 1537 Size: 275 1549 693 4090 Fragments arranged by size: 4090 1549 693 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2156 2265 2445 4537 1353 Size: 803 109 180 2092 3423 Fragments arranged by size: 3423 2092 803 180 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5194 5568 5714 387 Size: 1064 138 3605 374 146 1280 Fragments arranged by size: 3605 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2347 2347 Size: 6607 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2212 2363 2378 2639 2696 3133 3356 Size: 393 580 151 15 261 57 437 223 Cuts at: 3356 3880 4670 5267 6054 6322 1239 Size: 524 790 597 787 268 1524 Fragments arranged by size: 1524 790 787 597 580 524 437 393 268 261 223 151 57 15 EcoNI CCTnn'n_nnAGG Cuts at: 2678 3161 2678 Size: 483 6124 EcoO109I rG'GnC_Cy Cuts at: 2275 2652 2653 2761 3731 3732 3816 2275 Size: 377 1 108 970 1 84 5066 Fragments arranged by size: 5066 970 377 108 84 1 1 EcoRI G'AATT_C Cuts at: 3916 4294 3916 Size: 378 6229 FspI TGC'GCA Cuts at: 5113 5113 Size: 6607 HaeII r_GCGC'y Cuts at: 1678 2823 3259 5754 1678 Size: 1145 436 2495 2531 Fragments arranged by size: 2531 2495 1145 436 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2684 2716 2870 2902 3836 Size: 32 135 32 1112 32 154 32 934 Cuts at: 3836 3868 5248 5280 5322 5354 1373 Size: 32 1380 32 42 32 2626 Fragments arranged by size: 2626 1380 1112 934 154 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3951 4197 669 Size: 988 281 2013 246 3079 Fragments arranged by size: 3079 2013 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6607 HpaI GTT'AAC Cuts at: 4197 4197 Size: 6607 KpnI G_GTAC'C Cuts at: 3932 3932 Size: 6607 MfeI C'AATT_G Cuts at: 4206 4206 Size: 6607 MluI A'CGCG_T Cuts at: 6483 6483 Size: 6607 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2868 3048 5721 5905 1305 Size: 1563 180 2673 184 2007 Fragments arranged by size: 2673 2007 1563 184 180 MscI TGG'CCA Cuts at: 10 64 1489 1832 2816 10 Size: 54 1425 343 984 3801 Fragments arranged by size: 3801 1425 984 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3038 3623 4565 4924 5083 518 Size: 648 1872 585 942 359 159 2042 Fragments arranged by size: 2042 1872 942 648 585 359 159 MspA1I CmG'CkG Cuts at: 1606 2142 2746 2770 3138 3558 3672 3739 3813 Size: 536 604 24 368 420 114 67 74 Cuts at: 3813 4023 4700 5848 6093 1606 Size: 210 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 604 536 420 368 245 210 114 74 67 24 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5116 Fragments arranged by size: 5116 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6607 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6607 NotI GC'GGCC_GC Cuts at: 3361 4054 3361 Size: 693 5914 NspI r_CATG'y Cuts at: 1132 1229 3022 3965 5510 1132 Size: 97 1793 943 1545 2229 Fragments arranged by size: 2229 1793 1545 943 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6607 PciI A'CATG_T Cuts at: 5506 5506 Size: 6607 PflMI CCAn_nnn'nTGG Cuts at: 1108 3279 1108 Size: 2171 4436 PfoI T'CCnGG_A Cuts at: 2133 2133 Size: 6607 PmeI GTTT'AAAC Cuts at: 3911 3911 Size: 6607 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2006 2038 4676 4708 6189 6221 2006 Size: 32 2638 32 1481 32 2392 Fragments arranged by size: 2638 2392 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 2761 3816 2275 Size: 486 1055 5066 Fragments arranged by size: 5066 1055 486 PshAI GACnn'nnGTC Cuts at: 1613 3574 1613 Size: 1961 4646 PsiI TTA'TAA Cuts at: 4177 4177 Size: 6607 PspOMI G'GGCC_C Cuts at: 2652 3731 2652 Size: 1079 5528 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2066 2098 2066 Size: 32 6575 PstI C_TGCA'G Cuts at: 830 2769 2865 3162 3959 830 Size: 1939 96 297 797 3478 Fragments arranged by size: 3478 1939 797 297 96 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6607 PvuII CAG'CTG Cuts at: 3558 3739 3558 Size: 181 6426 SacI G_AGCT'C Cuts at: 2349 2349 Size: 6607 SacII CC_GC'GG Cuts at: 3673 3814 3673 Size: 141 6466 SalI G'TCGA_C Cuts at: 1936 3949 1936 Size: 2013 4594 SanDI GG'GwC_CC Cuts at: 3816 3816 Size: 6607 SbfI CC_TGCA'GG Cuts at: 3959 3959 Size: 6607 ScaI AGT'ACT Cuts at: 4855 6567 4855 Size: 1712 4895 SexAI A'CCwGG_T Cuts at: 3540 3540 Size: 6607 SfcI C'TryA_G Cuts at: 826 1045 2765 2849 2861 3158 3553 3955 5090 Size: 219 1720 84 12 297 395 402 1135 Cuts at: 5090 5771 5962 826 Size: 681 191 1471 Fragments arranged by size: 1720 1471 1135 681 402 395 297 219 191 84 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6607 SmaI CCC'GGG Cuts at: 1872 3934 1872 Size: 2062 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2494 2726 3299 3800 4711 5612 Size: 197 926 551 232 573 501 911 901 Cuts at: 5612 5874 820 Size: 262 1553 Fragments arranged by size: 1553 926 911 901 573 551 501 262 232 197 SnaBI TAC'GTA Cuts at: 493 4398 493 Size: 3905 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6607 SphI G_CATG'C Cuts at: 1132 3965 1132 Size: 2833 3774 SspI AAT'ATT Cuts at: 5 52 4531 5 Size: 47 4479 2081 Fragments arranged by size: 4479 2081 47 StuI AGG'CCT Cuts at: 1517 3759 1517 Size: 2242 4365 StyI C'CwwG_G Cuts at: 513 1065 2004 3085 4046 513 Size: 552 939 1081 961 3074 Fragments arranged by size: 3074 1081 961 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2042 2076 2431 2651 4634 4651 4804 Size: 1255 462 34 355 220 1983 17 153 Cuts at: 4804 4989 5434 325 Size: 185 445 1498 Fragments arranged by size: 1983 1498 1255 462 445 355 220 185 153 34 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2362 4853 6565 Size: 274 80 33 51 1443 385 2491 1712 Cuts at: 6565 96 Size: 138 Fragments arranged by size: 2491 1712 1443 385 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2276 2308 2882 2914 6567 6599 2276 Size: 32 574 32 3653 32 2284 Fragments arranged by size: 3653 2284 574 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6607 XbaI T'CTAG_A Cuts at: 3943 3943 Size: 6607 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3757 3757 Size: 6607 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6607 XmaI C'CCGG_G Cuts at: 1870 3932 1870 Size: 2062 4545 XmnI GAAnn'nnTTC Cuts at: 2993 4736 2993 Size: 1743 4864 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4415 276 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SapI SfiI SfoI SrfI SwaI