Restriction map for
pFN21AB5280
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglII
BlnI BmyI BpuAI BsaAI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 32 CGCG CG^CG
AlwI 555 GATCC GGATC(4/5)
Aor51HI 53 AGCGCT AGC^GCT
AvaII 6 GGACC G^G[AT]CC
BanI 160 GGTGCC G^G[CT][GA]CC
BglI 40 GCCGAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 616 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 616 GCTGAGC GC^T[ACGT]AGC
BsaBI 121 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 209 CCATGG C^C[ACGT][ACGT]GG
BseRI 130 CTCCTC GAGGAG(10/8)
BsiYI 132 CCTCTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 132 CCTCTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 5 GGGAC GGGAC(10/14)
BsmI 561 GAATGC GAATGC(1/-1)
BsrI 345 CCAGT ACTGG(1/-1)
BstUI 32 CGCG CG^CG
BstXI 509 CCATCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 554 AGATCC [GA]^GATC[CT]
CelII 616 GCTGAGC GC^T[ACGT]AGC
DsaI 209 CCATGG C^C[GA][CT]GG
EaeI 38 TGGCCG [CT]^GGCC[GA]
Eco47III 53 AGCGCT AGC^GCT
EcoT14I 209 CCATGG C^C[AT][AT]GG
FauI 27 GCGGG CCCGC(4/6)
HaeII 53 AGCGCT [GA]GCGC^[CT]
MaeIII 88 GTGAC ^GT[ACGT]AC
MamI 121 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 554 AGATCC [GA]^GATC[CT]
MseI 245 TTAA T^TAA
MvnI 32 CGCG CG^CG
NcoI 209 CCATGG C^CATGG
PleI 652 GACTC GAGTC(4/5)
PstI 22 CTGCAG CTGCA^G
PvuII 16 CAGCTG CAG^CTG
SfcI 22 CTGCAG C^T[GA][CT]AG
StyI 209 CCATGG C^C[AT][AT]GG
TfiI 640 GATTC G^A[AT]TC
Tru9I 245 TTAA T^TAA
Tsp45I 88 GTGAC ^GT[GC]AC
Tsp509I 233 AATT ^AATT
TspRI 432 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 611 CCATAGCTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 554 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwNI 16 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
670 CAGTTGCTG
BclI 323 TGATCA T^GATCA
662 TGATCA
BcnI 9 CCGGG CC^[GC]GG
255 CCGGG
BpmI 177 CTGGAG CTGGAG(16/14)
277 CTGGAG
BsrDI 290 GCAATG GCAATG(2/0)
335 GCAATG
BstAPI 15 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
669 GCAGTTGCTGC
CfoI 31 GCGC GCG^C
54 GCGC
FbaI 323 TGATCA T^GATCA
662 TGATCA
HhaI 31 GCGC GCG^C
54 GCGC
HinP1I 31 GCGC G^CGC
54 GCGC
HindIII 248 AAGCTT A^AGCTT
443 AAGCTT
HinfI 640 GATTC G^A[ACGT]TC
652 GACTC
MspA1I 16 CAGCTG C[AC]G^C[GT]G
25 CAGCGG
NciI 9 CCGGG CC^[GC]GG
255 CCGGG
NlaIV 5 GGGACC GG[ACGT]^[ACGT]CC
160 GGTGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BbvI BsmAI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MboI
MboII MnlI MspI MvaI MwoI
NdeII NlaIII Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TthHB8I