Restriction map for
pFN21AB5231
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BclI BfrI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstYI
BstZ17I CelII CfoI Cfr10I CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MroI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 1843 GTAGAC GT^[AC][GT]AC
AccII 12 CGCG CG^CG
AlwI 1684 GGATC GGATC(4/5)
AocI 1939 CCTAAGG CC^T[ACGT]AGG
Asp700I 1498 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 851 TGGCCA TGG^CCA
BcgI 984 CGATGTTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1743 GGATAC GTATCC(6/5)
BsmI 414 GAATGC GAATGC(1/-1)
BspDI 1360 ATCGAT AT^CGAT
BsrDI 1118 GCAATG GCAATG(2/0)
BstBI 1597 TTCGAA TT^CGAA
BstUI 12 CGCG CG^CG
Bsu36I 1939 CCTAAGG CC^T[ACGT]AGG
ClaI 1360 ATCGAT AT^CGAT
DraI 1617 TTTAAA TTT^AAA
DsaI 1886 CCATGG C^C[GA][CT]GG
EaeI 851 TGGCCA [CT]^GGCC[GA]
Eco81I 1939 CCTAAGG CC^T[ACGT]AGG
EcoNI 1734 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1574 ATGCAT ATGCA^T
HgaI 1603 GACGC GACGC(5/10)
HindIII 1613 AAGCTT A^AGCTT
MaeII 692 ACGT A^CGT
MluNI 851 TGGCCA TGG^CCA
MscI 851 TGGCCA TGG^CCA
MvnI 12 CGCG CG^CG
NcoI 1886 CCATGG C^CATGG
NlaIII 1887 CATG CATG^
NruI 11 TCGCGA TCG^CGA
NsiI 1574 ATGCAT ATGCA^T
NspV 1597 TTCGAA TT^CGAA
SapI 808 GAAGAGC GCTCTTC(1/4)
SfuI 1597 TTCGAA TT^CGAA
StuI 1731 AGGCCT AGG^CCT
XmnI 1498 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AciI 1249 GCGG CCGC(-3/-1)
1982 GCGG
AfaI 1383 GTAC GT^AC
1540 GTAC
AvaII 1675 GGACC G^G[AT]CC
1820 GGTCC
BbvCI 176 CCTCAGC CCTCAGC(-5/-2)
697 GCTGAGG
BcnI 1809 CCGGG CC^[GC]GG
2040 CCGGG
BfaI 1037 CTAG C^TAG
1132 CTAG
BsmAI 634 GAGAC GTCTC(1/5)
1756 GAGAC
BspMI 276 ACCTGC ACCTGC(4/8)
19 GCAGGT
BstEII 34 GGTGACC G^GT[ACGT]ACC
2092 GGTGACC
BstPI 34 GGTGACC G^GT[ACGT]ACC
2092 GGTGACC
BstXI 41 CCAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
686 CCAAGAACGTGG
Cac8I 9 GCTCGC GC[ACGT]^[ACGT]GC
1703 GCCAGC
DraII 1674 AGGACCC [GA]G^G[ACGT]CC[CT]
1819 GGGTCCT
EcoO109I 1674 AGGACCC [GA]G^G[ACGT]CC[CT]
1819 GGGTCCT
EcoO65I 34 GGTGACC G^GT[ACGT]ACC
2092 GGTGACC
HapII 1809 CCGG C^CGG
2040 CCGG
HpaII 1809 CCGG C^CGG
2040 CCGG
MaeI 1037 CTAG C^TAG
1132 CTAG
MspA1I 242 CAGCTG C[AC]G^C[GT]G
533 CAGCTG
MspI 1809 CCGG C^CGG
2040 CCGG
NciI 1809 CCGGG CC^[GC]GG
2040 CCGGG
PleI 613 GACTC GAGTC(4/5)
1768 GAGTC
PpuMI 1674 AGGACCC [GA]G^G[AT]CC[CT]
1819 GGGTCCT
PvuII 242 CAGCTG CAG^CTG
533 CAGCTG
RsaI 1383 GTAC GT^AC
1540 GTAC
SfcI 1416 CTATAG C^T[GA][CT]AG
1482 CTACAG
TaqI 1361 TCGA T^CGA
1598 TCGA
TfiI 1063 GAATC G^A[AT]TC
1450 GAATC
TthHB8I 1361 TCGA T^CGA
1598 TCGA
XcmI 1545 CCAGAACCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1877 CCAAGGAGGCCATGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AcsI AluI AlwNI ApoI BbsI
BbvI BglI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsrI BssKI
BstAPI BstF5I BstNI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI SfaNI StyI
Tru9I TseI Tsp45I Tsp509I TspRI