(Circular) (Six-base) MAPSORT of: pFN21AB5231.seq Check: 1491 from: 1 to: 6835 pFN21AB5231 6835 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2290 2290 Size: 6835 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4645 278 Size: 53 83 186 936 3109 2468 Fragments arranged by size: 3109 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3849 4178 1937 Size: 1912 329 4594 Fragments arranged by size: 4594 1912 329 Acc65I G'GTAC_C Cuts at: 4156 4156 Size: 6835 AclI AA'CG_TT Cuts at: 1563 4962 5335 1563 Size: 3399 373 3063 Fragments arranged by size: 3399 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2643 2898 3096 3494 3777 4898 6282 6550 Size: 1011 255 198 398 283 1121 1384 268 Cuts at: 6550 1632 Size: 1917 Fragments arranged by size: 1917 1384 1121 1011 398 283 268 255 198 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6638 AflIII A'CryG_T Cuts at: 1203 5734 6711 1203 Size: 4531 977 1327 Fragments arranged by size: 4531 1327 977 AgeI A'CCGG_T Cuts at: 5666 5666 Size: 6835 AhdI GACnn_n'nnGTC Cuts at: 5564 5564 Size: 6835 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6835 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6835 AlwNI CAG_nnn'CTG Cuts at: 2540 2543 2876 3323 4578 6150 2540 Size: 3 333 447 1255 1572 3225 Fragments arranged by size: 3225 1572 1255 447 333 3 ApaLI G'TGCA_C Cuts at: 4892 6048 4892 Size: 1156 5679 ApoI r'AATT_y Cuts at: 1493 3047 3308 3625 4144 4370 4522 6631 1493 Size: 1554 261 317 519 226 152 2109 1697 Fragments arranged by size: 2109 1697 1554 519 317 261 226 152 AseI AT'TA_AT Cuts at: 160 1032 5389 160 Size: 872 4357 1606 Fragments arranged by size: 4357 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6835 AvaI C'yCGr_G Cuts at: 1870 1943 4160 1870 Size: 73 2217 4545 Fragments arranged by size: 4545 2217 73 BamHI G'GATC_C Cuts at: 4165 4532 4165 Size: 367 6468 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4156 5611 618 Size: 325 217 627 2369 1455 1842 Fragments arranged by size: 2369 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4154 1391 Size: 2763 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2297 2439 3084 3612 3903 4127 928 Size: 971 398 142 645 528 291 224 3636 Fragments arranged by size: 3636 971 645 528 398 291 224 142 BbvCI CC'TCA_GC Cuts at: 2182 2703 2182 Size: 521 6314 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2978 3012 5026 5060 1903 Size: 34 1041 34 2014 34 3678 Fragments arranged by size: 3678 2014 1041 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3742 4727 5937 3742 Size: 985 1210 4640 Fragments arranged by size: 4640 1210 985 BclI T'GATC_A Cuts at: 1548 1548 Size: 6835 BfrBI ATG'CAT Cuts at: 3581 3581 Size: 6835 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2402 3887 4050 Size: 107 122 71 631 605 730 1485 163 Cuts at: 4050 5446 136 Size: 1396 1525 Fragments arranged by size: 1525 1485 1396 730 631 605 163 122 107 71 BglII A'GATC_T Cuts at: 6830 6830 Size: 6835 BlpI GC'TnA_GC Cuts at: 4252 4252 Size: 6835 Bme1580I G_kGCm'C Cuts at: 1790 4896 6052 1790 Size: 3106 1156 2573 Fragments arranged by size: 3106 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6835 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3831 3943 4566 Size: 416 53 83 399 24 2414 112 623 Cuts at: 4566 5524 442 Size: 958 1753 Fragments arranged by size: 2414 1753 958 623 416 399 112 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6835 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2241 3104 3417 3689 5495 1239 Size: 1002 863 313 272 1806 2579 Fragments arranged by size: 2579 1806 1002 863 313 272 Bpu10I CC'TnA_GC Cuts at: 2182 2703 2182 Size: 521 6314 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4960 5825 6123 4960 Size: 865 298 5672 Fragments arranged by size: 5672 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5498 882 Size: 617 3999 2219 Fragments arranged by size: 3999 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4626 493 Size: 711 3422 2702 Fragments arranged by size: 3422 2702 711 BsaBI GATnn'nnATC Cuts at: 6829 6829 Size: 6835 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4642 5024 5659 275 Size: 53 83 186 936 3109 382 635 1451 Fragments arranged by size: 3109 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5268 5666 5940 6087 6498 5268 Size: 398 274 147 411 5605 Fragments arranged by size: 5605 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2124 2154 3077 3107 1178 Size: 30 165 30 721 30 923 30 4906 Fragments arranged by size: 4906 923 721 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2049 2052 2462 2480 2489 2804 2807 2819 Size: 863 3 410 18 9 315 3 12 Cuts at: 2819 2822 3878 4029 4038 4080 4092 4101 4104 Size: 3 1056 151 9 42 12 9 3 Cuts at: 4104 4107 1186 Size: 3 3914 Fragments arranged by size: 3914 1056 863 410 315 151 42 18 12 12 9 9 9 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2392 6038 6534 2392 Size: 3646 496 2693 Fragments arranged by size: 3646 2693 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6262 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4285 5046 6074 1540 Size: 275 186 2284 761 1028 2301 Fragments arranged by size: 2301 2284 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4896 4981 6052 4896 Size: 85 1071 5679 Fragments arranged by size: 5679 1071 85 BsmI GAATG_Cn' Cuts at: 2425 4346 4439 2425 Size: 1921 93 4821 Fragments arranged by size: 4821 1921 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4154 4896 4981 6052 1391 Size: 399 2364 742 85 1071 2174 Fragments arranged by size: 2364 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6498 6498 Size: 6835 BspHI T'CATG_A Cuts at: 4724 6454 4724 Size: 1730 5105 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2015 2290 4190 844 Size: 1171 275 1900 3489 Fragments arranged by size: 3489 1900 1171 275 BsrBI CCG'CTC Cuts at: 1394 1823 4722 1394 Size: 429 2899 3507 Fragments arranged by size: 3507 2899 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3130 5330 5504 65 Size: 1183 1882 2200 174 1396 Fragments arranged by size: 2200 1882 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5479 5666 1700 Size: 110 3669 187 2869 Fragments arranged by size: 3669 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6835 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6835 BssSI C'ACGA_G Cuts at: 4895 5907 4895 Size: 1012 5823 BstAPI GCAn_nnn'nTGC Cuts at: 794 2282 2540 2543 794 Size: 1488 258 3 5086 Fragments arranged by size: 5086 1488 258 3 BstBI TT'CG_AA Cuts at: 3603 5639 3603 Size: 2036 4799 BstEII G'GTnAC_C Cuts at: 1184 2039 4097 1184 Size: 855 2058 3922 Fragments arranged by size: 3922 2058 855 BstXI CCAn_nnnn'nTGG Cuts at: 2053 2698 2053 Size: 645 6190 BstYI r'GATC_y Cuts at: 1972 4165 4532 4917 4934 6386 6472 6484 6830 Size: 2193 367 385 17 1452 86 12 346 Cuts at: 6830 1972 Size: 1977 Fragments arranged by size: 2193 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3945 3945 Size: 6835 BtgI C'CryG_G Cuts at: 513 1065 2004 3891 513 Size: 552 939 1887 3457 Fragments arranged by size: 3457 1887 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2540 3003 487 Size: 683 56 1314 463 4319 Fragments arranged by size: 4319 1314 683 463 56 BtsI GCAGTG_nn' Cuts at: 2134 4351 5145 5165 2134 Size: 2217 794 20 3804 Fragments arranged by size: 3804 2217 794 20 ClaI AT'CG_AT Cuts at: 1554 3366 1554 Size: 1812 5023 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2175 2210 529 Size: 35 1611 35 5154 Fragments arranged by size: 5154 1611 35 35 DraI TTT'AAA Cuts at: 3624 4139 3624 Size: 515 6320 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5842 809 Size: 507 4526 1802 Fragments arranged by size: 4526 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2856 4282 5171 Size: 54 1425 50 275 18 1026 1426 889 Cuts at: 5171 6800 8 Size: 1629 43 Fragments arranged by size: 1629 1426 1425 1026 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4282 1537 Size: 275 2470 4090 Fragments arranged by size: 4090 2470 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2130 2439 2715 2796 2808 3513 3753 4765 Size: 777 309 276 81 12 705 240 1012 Cuts at: 4765 1353 Size: 3423 Fragments arranged by size: 3423 1012 777 705 309 276 240 81 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5422 5796 5942 387 Size: 1064 138 3833 374 146 1280 Fragments arranged by size: 3833 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2241 2643 2898 3096 3104 3417 3494 Size: 393 609 402 255 198 8 313 77 Cuts at: 3494 3689 3777 4898 5495 6282 6550 1239 Size: 195 88 1121 597 787 268 1524 Fragments arranged by size: 1524 1121 787 609 597 402 393 313 268 255 198 195 88 77 8 EcoNI CCTnn'n_nnAGG Cuts at: 3743 3743 Size: 6835 EcoO109I rG'GnC_Cy Cuts at: 3680 3825 3680 Size: 145 6690 EcoRI G'AATT_C Cuts at: 4144 4522 4144 Size: 378 6457 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3524 3524 Size: 6835 FspI TGC'GCA Cuts at: 5341 5341 Size: 6835 HaeII r_GCGC'y Cuts at: 1678 5982 1678 Size: 4304 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 2124 2156 3053 Size: 32 135 32 515 32 5 32 897 Cuts at: 3053 3085 3359 3391 5476 5508 5550 5582 1373 Size: 32 274 32 2085 32 42 32 2626 Fragments arranged by size: 2626 2085 897 515 274 135 42 32 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 4179 4425 669 Size: 988 281 2241 246 3079 Fragments arranged by size: 3079 2241 988 281 246 HindIII A'AGCT_T Cuts at: 748 3618 748 Size: 2870 3965 HpaI GTT'AAC Cuts at: 4425 4425 Size: 6835 KpnI G_GTAC'C Cuts at: 4160 4160 Size: 6835 MfeI C'AATT_G Cuts at: 4434 4434 Size: 6835 MluI A'CGCG_T Cuts at: 6711 6711 Size: 6835 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2917 3075 5949 6133 1305 Size: 1612 158 2874 184 2007 Fragments arranged by size: 2874 2007 1612 184 158 MscI TGG'CCA Cuts at: 10 64 1489 1832 2858 10 Size: 54 1425 343 1026 3987 Fragments arranged by size: 3987 1425 1026 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4793 5152 5311 518 Size: 648 3627 359 159 2042 Fragments arranged by size: 3627 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2249 2540 4251 4928 6076 6321 1606 Size: 643 291 1711 677 1148 245 2120 Fragments arranged by size: 2120 1711 1148 677 643 291 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3891 513 Size: 552 939 1887 3457 Fragments arranged by size: 3457 1887 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6835 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6835 NotI GC'GGCC_GC Cuts at: 4282 4282 Size: 6835 NruI TCG'CGA Cuts at: 2018 2018 Size: 6835 NsiI A_TGCA'T Cuts at: 3583 3583 Size: 6835 NspI r_CATG'y Cuts at: 1132 1229 4193 5738 1132 Size: 97 2964 1545 2229 Fragments arranged by size: 2964 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3684 1348 Size: 2336 4499 PciI A'CATG_T Cuts at: 5734 5734 Size: 6835 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6835 PmeI GTTT'AAAC Cuts at: 4139 4139 Size: 6835 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4904 4936 6417 6449 4904 Size: 32 1481 32 5290 Fragments arranged by size: 5290 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3680 3825 3680 Size: 145 6690 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6835 PsiI TTA'TAA Cuts at: 4405 4405 Size: 6835 PstI C_TGCA'G Cuts at: 830 4187 830 Size: 3357 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6835 PvuII CAG'CTG Cuts at: 2249 2540 2249 Size: 291 6544 SalI G'TCGA_C Cuts at: 1936 4177 1936 Size: 2241 4594 SapI GCTCTTCn'nnn_ Cuts at: 2808 2808 Size: 6835 SbfI CC_TGCA'GG Cuts at: 4187 4187 Size: 6835 ScaI AGT'ACT Cuts at: 5083 6795 5083 Size: 1712 5123 SfcI C'TryA_G Cuts at: 826 1045 3421 3487 4183 5318 5999 6190 826 Size: 219 2376 66 696 1135 681 191 1471 Fragments arranged by size: 2376 1471 1135 696 681 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6835 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6835 SmaI CCC'GGG Cuts at: 1872 4162 1872 Size: 2290 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4939 5840 6102 820 Size: 197 926 2996 901 262 1553 Fragments arranged by size: 2996 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4626 493 Size: 4133 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6835 SphI G_CATG'C Cuts at: 1132 4193 1132 Size: 3061 3774 SspI AAT'ATT Cuts at: 5 52 4759 5 Size: 47 4707 2081 Fragments arranged by size: 4707 2081 47 StuI AGG'CCT Cuts at: 1517 3738 1517 Size: 2221 4614 StyI C'CwwG_G Cuts at: 513 1065 2004 2397 3882 3891 3927 4274 513 Size: 552 939 393 1485 9 36 347 3074 Fragments arranged by size: 3074 1485 939 552 393 347 36 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4862 4879 5032 5217 5662 325 Size: 1255 3282 17 153 185 445 1498 Fragments arranged by size: 3282 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3387 5081 6793 Size: 274 80 33 51 1443 1410 1694 1712 Cuts at: 6793 96 Size: 138 Fragments arranged by size: 1712 1694 1443 1410 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6795 6827 6795 Size: 32 6803 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6835 XbaI T'CTAG_A Cuts at: 4171 4171 Size: 6835 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3557 3889 3557 Size: 332 6503 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6835 XmaI C'CCGG_G Cuts at: 1870 4160 1870 Size: 2290 4545 XmnI GAAnn'nnTTC Cuts at: 3507 4964 3507 Size: 1457 5378 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4643 276 Size: 53 83 186 936 3109 2468 Fragments arranged by size: 3109 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI