Restriction map for
pFN21AB5231
(Linear) (Six-base) MAPSORT of: KIBB5231.seq Check: 8586 from: 1 to: 2130
KIBB5231.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 285 2130
Size: 285 1845
AccI GT'mk_AC
Cuts at: 0 1844 2130
Size: 1844 286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 893 1091 1489 1772 2130
Size: 638 255 198 398 283 358
Fragments arranged by size:
638 398 358 283 255 198
AlwNI CAG_nnn'CTG
Cuts at: 0 535 538 871 1318 2130
Size: 535 3 333 447 812
Fragments arranged by size:
812 535 447 333 3
ApoI r'AATT_y
Cuts at: 0 1042 1303 1620 2130
Size: 1042 261 317 510
Fragments arranged by size:
1042 510 317 261
BbsI GAAGACnn'nnnn_
Cuts at: 0 292 434 1079 1607 1898 2122 2130
Size: 292 142 645 528 291 224 8
Fragments arranged by size:
645 528 292 291 224 142 8
BbvCI CC'TCA_GC
Cuts at: 0 177 698 2130
Size: 177 521 1432
Fragments arranged by size:
1432 521 177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 973 1007 2130
Size: 973 34 1123
Fragments arranged by size:
1123 973 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1737 2130
Size: 1737 393
BfrBI ATG'CAT
Cuts at: 0 1576 2130
Size: 1576 554
BglI GCCn_nnn'nGGC
Cuts at: 0 397 1882 2045 2130
Size: 397 1485 163 85
Fragments arranged by size:
1485 397 163 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 1826 1938 2130
Size: 1826 112 192
Fragments arranged by size:
1826 192 112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 1099 1412 1684 2130
Size: 236 863 313 272 446
Fragments arranged by size:
863 446 313 272 236
Bpu10I CC'TnA_GC
Cuts at: 0 177 698 2130
Size: 177 521 1432
Fragments arranged by size:
1432 521 177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 119 149 1072 1102 2130
Size: 119 30 923 30 1028
Fragments arranged by size:
1028 923 119 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 44 47 457 475 484 799 802 814
Size: 44 3 410 18 9 315 3 12
Cuts at: 814 817 1873 2024 2033 2075 2087 2096 2099
Size: 3 1056 151 9 42 12 9 3
Cuts at: 2099 2102 2130
Size: 3 28
Fragments arranged by size:
1056 410 315 151 44 42 28 18
12 12 9 9 9 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 387 2130
Size: 387 1743
BsmI GAATG_Cn'
Cuts at: 0 420 2130
Size: 420 1710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 10 285 2130
Size: 10 275 1845
Fragments arranged by size:
1845 275 10
BsrDI GCAATG_nn'
Cuts at: 0 1125 2130
Size: 1125 1005
BstAPI GCAn_nnn'nTGC
Cuts at: 0 277 535 538 2130
Size: 277 258 3 1592
Fragments arranged by size:
1592 277 258 3
BstBI TT'CG_AA
Cuts at: 0 1598 2130
Size: 1598 532
BstEII G'GTnAC_C
Cuts at: 0 34 2092 2130
Size: 34 2058 38
Fragments arranged by size:
2058 38 34
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 693 2130
Size: 48 645 1437
Fragments arranged by size:
1437 645 48
Bsu36I CC'TnA_GG
Cuts at: 0 1940 2130
Size: 1940 190
BtgI C'CryG_G
Cuts at: 0 1886 2130
Size: 1886 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 535 998 2130
Size: 535 463 1132
Fragments arranged by size:
1132 535 463
BtsI GCAGTG_nn'
Cuts at: 0 129 2130
Size: 129 2001
ClaI AT'CG_AT
Cuts at: 0 1361 2130
Size: 1361 769
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 170 205 2130
Size: 170 35 1925
Fragments arranged by size:
1925 170 35
DraI TTT'AAA
Cuts at: 0 1619 2130
Size: 1619 511
EaeI y'GGCC_r
Cuts at: 0 851 2130
Size: 851 1279
EarI CTCTTCn'nnn_
Cuts at: 0 125 434 710 791 803 1508 1748 2130
Size: 125 309 276 81 12 705 240 382
Fragments arranged by size:
705 382 309 276 240 125 81 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 638 893 1091 1099 1412 1489 1684
Size: 236 402 255 198 8 313 77 195
Cuts at: 1684 1772 2130
Size: 88 358
Fragments arranged by size:
402 358 313 255 236 198 195 88
77 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1738 2130
Size: 1738 392
EcoO109I rG'GnC_Cy
Cuts at: 0 1675 1820 2130
Size: 1675 145 310
Fragments arranged by size:
1675 310 145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1519 2130
Size: 1519 611
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 119 151 1048 1080 1354 1386
Size: 82 32 5 32 897 32 274 32
Cuts at: 1386 2130
Size: 744
Fragments arranged by size:
897 744 274 82 32 32 32 32
5
HindIII A'AGCT_T
Cuts at: 0 1613 2130
Size: 1613 517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 912 1070 2130
Size: 912 158 1060
Fragments arranged by size:
1060 912 158
MscI TGG'CCA
Cuts at: 0 853 2130
Size: 853 1277
MspA1I CmG'CkG
Cuts at: 0 244 535 2130
Size: 244 291 1595
Fragments arranged by size:
1595 291 244
NcoI C'CATG_G
Cuts at: 0 1886 2130
Size: 1886 244
NruI TCG'CGA
Cuts at: 0 13 2130
Size: 13 2117
NsiI A_TGCA'T
Cuts at: 0 1578 2130
Size: 1578 552
PasI CC'CwG_GG
Cuts at: 0 1679 2130
Size: 1679 451
PpuMI rG'GwC_Cy
Cuts at: 0 1675 1820 2130
Size: 1675 145 310
Fragments arranged by size:
1675 310 145
PvuII CAG'CTG
Cuts at: 0 244 535 2130
Size: 244 291 1595
Fragments arranged by size:
1595 291 244
SapI GCTCTTCn'nnn_
Cuts at: 0 803 2130
Size: 803 1327
SfcI C'TryA_G
Cuts at: 0 1416 1482 2130
Size: 1416 66 648
Fragments arranged by size:
1416 648 66
StuI AGG'CCT
Cuts at: 0 1733 2130
Size: 1733 397
StyI C'CwwG_G
Cuts at: 0 392 1877 1886 1922 2130
Size: 392 1485 9 36 208
Fragments arranged by size:
1485 392 208 36 9
TatI w'GTAC_w
Cuts at: 0 1382 2130
Size: 1382 748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1552 1884 2130
Size: 1552 332 246
Fragments arranged by size:
1552 332 246
XmnI GAAnn'nnTTC
Cuts at: 0 1502 2130
Size: 1502 628
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI BbsI BbvCI BcgI
BciVI BfrBI BglI BmrI BpmI Bpu10I BsaXI BseRI
BseYI BsmI BspMI BsrDI BstAPI BstBI BstEII BstXI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI EaeI
EarI Eco57MI EcoNI EcoO109I FalI Hin4I HindIII MmeI
MscI MspA1I NcoI NruI NsiI PasI PpuMI PvuII
SapI SfcI StuI StyI TatI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BclI
BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstYI BstZ17I DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MslI NaeI NarI NdeI
NgoMIV NheI NotI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI ZraI