Restriction map for
pFN21AB5227
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BcgI BclI
BcnI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI Bsu36I Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MluI MluNI MroI
MscI MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 698 GAATTC [GA]^AATT[CT]
ApoI 698 GAATTC [GA]^AATT[CT]
AseI 486 ATTAAT AT^TAAT
AsnI 486 ATTAAT AT^TAAT
Asp700I 247 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 455 GAGCAC G[AT]GC[AT]^C
BanI 1404 GGTGCC G^G[CT][GA]CC
BbvCI 400 CCTCAGC CCTCAGC(-5/-2)
BciVI 1357 GGATAC GTATCC(6/5)
BlpI 146 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 146 GCTTAGC GC^T[ACGT]AGC
BsgI 758 CTGCAC GTGCAG(16/14)
BsiHKAI 455 GAGCAC G[AT]GC[AT]^C
BsrDI 157 CATTGC GCAATG(2/0)
Bst1107I 132 GTATAC GTA^TAC
BstYI 71 AGATCC [GA]^GATC[CT]
BstZ17I 132 GTATAC GTA^TAC
CelII 146 GCTTAGC GC^T[ACGT]AGC
CfoI 733 GCGC GCG^C
DraI 103 TTTAAA TTT^AAA
DsaI 31 CCGTGG C^C[GA][CT]GG
EarI 1165 GAAGAG CTCTTC(1/4)
EcoRI 698 GAATTC G^AATTC
EcoT14I 936 CCAAGG C^C[AT][AT]GG
HaeII 732 AGCGCC [GA]GCGC^[CT]
HhaI 733 GCGC GCG^C
HinP1I 733 GCGC G^CGC
HphI 589 GGTGA GGTGA(8/7)
Ksp632I 1165 GAAGAG CTCTTC(1/4)
MfeI 1250 CAATTG C^AATTG
MflI 71 AGATCC [GA]^GATC[CT]
MunI 1250 CAATTG C^AATTG
PshAI 834 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 486 ATTAAT AT^TAAT
ScaI 1060 AGTACT AGT^ACT
SexAI 666 ACCAGGT A^CC[AT]GGT
StyI 936 CCAAGG C^C[AT][AT]GG
TaqI 1431 TCGA T^CGA
TfiI 334 GATTC G^A[AT]TC
TspRI 816 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 1431 TCGA T^CGA
XcmI 936 CCAAGGACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 71 AGATCC [GA]^GATC[CT]
XmnI 247 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 132 GTATAC GT^[AC][GT]AC
1029 GTCTAC
AlwI 72 GATCC GGATC(4/5)
1137 GATCC
AlwNI 137 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
767 CAGCAACTG
BpmI 470 CTCCAG CTGGAG(16/14)
791 CTCCAG
BsaI 1302 GAGACC GGTCTC(1/5)
1449 GAGACC
BseRI 503 CTCCTC GAGGAG(10/8)
1427 CTCCTC
BsmAI 1302 GAGAC GTCTC(1/5)
1449 GAGAC
BsmFI 832 GGGAC GGGAC(10/14)
1392 GGGAC
DpnII 72 GATC ^GATC
1137 GATC
EaeI 1210 CGGCCA [CT]^GGCC[GA]
1473 TGGCCG
MaeII 690 ACGT A^CGT
1197 ACGT
MboI 72 GATC ^GATC
1137 GATC
MslI 597 CATAATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1141 CATCCAGATG
NdeII 72 GATC ^GATC
1137 GATC
PleI 358 GACTC GAGTC(4/5)
711 GACTC
PpuMI 138 AGGACCT [GA]G^G[AT]CC[CT]
939 AGGACCT
PstI 362 CTGCAG CTGCA^G
1064 CTGCAG
PvuII 22 CAGCTG CAG^CTG
929 CAGCTG
Sau3AI 72 GATC ^GATC
1137 GATC
SmlI 713 CTCAAG C^T[CT][GA]AG
849 CTCAAG
SpeI 1045 ACTAGT A^CTAGT
1117 ACTAGT
Tsp45I 119 GTCAC ^GT[GC]AC
841 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EcoNI EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MaeIII MboII MnlI MseI MspA1I
MvaI MwoI NlaIII NlaIV NspI
PflMI RsaI Sau96I ScrFI SfaNI
SfcI SphI Tru9I TseI Tsp509I
Van91I