(Circular) (Six-base) MAPSORT of: pFN21AB5227.seq Check: 8072 from: 1 to: 6187 pFN21AB5227 6187 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2770 2770 Size: 6187 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2138 3035 3530 1937 Size: 201 897 495 4594 Fragments arranged by size: 4594 897 495 201 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2051 4250 5634 5902 1632 Size: 419 2199 1384 268 1917 Fragments arranged by size: 2199 1917 1384 419 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 5086 6063 1203 Size: 3883 977 1327 Fragments arranged by size: 3883 1327 977 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6187 AlwNI CAG_nnn'CTG Cuts at: 2147 2777 3930 5502 2147 Size: 630 1153 1572 2832 Fragments arranged by size: 2832 1572 1153 630 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 2703 3496 3722 3874 5983 1493 Size: 1210 793 226 152 2109 1697 Fragments arranged by size: 2109 1697 1210 793 226 152 AseI AT'TA_AT Cuts at: 160 1032 2492 4741 160 Size: 872 1460 2249 1606 Fragments arranged by size: 2249 1606 1460 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 3512 1870 Size: 73 1569 4545 Fragments arranged by size: 4545 1569 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3179 3212 3179 Size: 33 6154 BamHI G'GATC_C Cuts at: 3517 3884 3517 Size: 367 5820 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3409 3508 4963 618 Size: 325 217 627 1622 99 1455 1842 Fragments arranged by size: 1842 1622 1455 627 325 217 99 BanII G_rGCy'C Cuts at: 1391 3506 1391 Size: 2115 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5216 BbvCI CC'TCA_GC Cuts at: 2406 2406 Size: 6187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4378 4412 1903 Size: 34 2441 34 3678 Fragments arranged by size: 3678 2441 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3356 4079 5289 3356 Size: 723 1210 4254 Fragments arranged by size: 4254 1210 723 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4798 136 Size: 107 122 71 631 605 3126 1525 Fragments arranged by size: 3126 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6182 6182 Size: 6187 BlpI GC'TnA_GC Cuts at: 2152 3604 2152 Size: 1452 4735 Bme1580I G_kGCm'C Cuts at: 1790 3184 3414 4248 5404 1790 Size: 1394 230 834 1156 2573 Fragments arranged by size: 2573 1394 1156 834 230 BmgBI CAC'GTC Cuts at: 1318 2696 1318 Size: 1378 4809 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2575 2814 3918 Size: 416 53 83 399 24 1158 239 1104 Cuts at: 3918 4876 442 Size: 958 1753 Fragments arranged by size: 1753 1158 1104 958 416 399 239 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2460 2781 4847 1239 Size: 1221 321 2066 2579 Fragments arranged by size: 2579 2066 1221 321 Bpu10I CC'TnA_GC Cuts at: 2406 2928 2406 Size: 522 5665 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2703 2839 4312 5177 5475 2703 Size: 136 1473 865 298 3415 Fragments arranged by size: 3415 1473 865 298 136 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3301 3448 4850 882 Size: 617 1802 147 1402 2219 Fragments arranged by size: 2219 1802 1402 617 147 BsaAI yAC'GTr Cuts at: 493 1204 3978 493 Size: 711 2774 2702 Fragments arranged by size: 2774 2702 711 BsaBI GATnn'nnATC Cuts at: 6181 6181 Size: 6187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3994 4376 5011 275 Size: 53 83 186 936 2461 382 635 1451 Fragments arranged by size: 2461 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4620 5018 5292 5439 5850 4620 Size: 398 274 147 411 4957 Fragments arranged by size: 4957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5962 Fragments arranged by size: 5962 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2499 3423 1186 Size: 1313 924 3950 Fragments arranged by size: 3950 1313 924 BseYI C'CCAG_C Cuts at: 5390 5886 5390 Size: 496 5691 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2748 1163 Size: 573 1012 4602 Fragments arranged by size: 4602 1012 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3637 4398 5426 1540 Size: 275 186 1636 761 1028 2301 Fragments arranged by size: 2301 1636 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2464 4248 4333 5404 2464 Size: 1784 85 1071 3247 Fragments arranged by size: 3247 1784 1071 85 BsmI GAATG_Cn' Cuts at: 2475 2529 2670 3698 3791 2475 Size: 54 141 1028 93 4871 Fragments arranged by size: 4871 1028 141 93 54 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2464 3184 3414 3506 4248 4333 5404 Size: 399 674 720 230 92 742 85 1071 Cuts at: 5404 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 720 674 399 230 92 85 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 4076 5806 4076 Size: 1730 4457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2161 2770 2791 3542 844 Size: 1317 609 21 751 3489 Fragments arranged by size: 3489 1317 751 609 21 BsrBI CCG'CTC Cuts at: 1394 1823 4074 1394 Size: 429 2251 3507 Fragments arranged by size: 3507 2251 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2161 4682 4856 65 Size: 1183 913 2521 174 1396 Fragments arranged by size: 2521 1396 1183 913 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4831 5018 1700 Size: 110 3021 187 2869 Fragments arranged by size: 3021 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6187 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6187 BssSI C'ACGA_G Cuts at: 4247 5259 4247 Size: 1012 5175 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6187 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstYI r'GATC_y Cuts at: 1972 2076 3517 3884 4269 4286 5738 5824 5836 Size: 104 1441 367 385 17 1452 86 12 Cuts at: 5836 6182 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1441 385 367 346 104 86 17 12 BstZ17I GTA'TAC Cuts at: 2139 2139 Size: 6187 BtgI C'CryG_G Cuts at: 513 1065 2004 2036 513 Size: 552 939 32 4664 Fragments arranged by size: 4664 939 552 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5448 Fragments arranged by size: 5448 683 56 BtsI GCAGTG_nn' Cuts at: 3703 4497 4517 3703 Size: 794 20 5373 Fragments arranged by size: 5373 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2538 2573 529 Size: 35 1974 35 4143 Fragments arranged by size: 4143 1974 35 35 DraI TTT'AAA Cuts at: 2110 3491 2110 Size: 1381 4806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3215 3478 3634 Size: 54 1425 50 275 18 1385 263 156 Cuts at: 3634 4523 6152 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1385 889 275 263 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3634 1537 Size: 275 1822 4090 Fragments arranged by size: 4090 1822 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3165 4117 1353 Size: 1812 952 3423 Fragments arranged by size: 3423 1812 952 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4774 5148 5294 387 Size: 1064 138 3185 374 146 1280 Fragments arranged by size: 3185 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2460 2781 4250 4847 5634 5902 Size: 393 419 409 321 1469 597 787 268 Cuts at: 5902 1239 Size: 1524 Fragments arranged by size: 1524 1469 787 597 419 409 393 321 268 EcoNI CCTnn'n_nnAGG Cuts at: 2409 2637 3216 2409 Size: 228 579 5380 Fragments arranged by size: 5380 579 228 EcoO109I rG'GnC_Cy Cuts at: 2144 2945 3226 2144 Size: 801 281 5105 Fragments arranged by size: 5105 801 281 EcoRI G'AATT_C Cuts at: 2703 3496 3874 2703 Size: 793 378 5016 Fragments arranged by size: 5016 793 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2880 2880 Size: 6187 FspI TGC'GCA Cuts at: 4693 4693 Size: 6187 HaeII r_GCGC'y Cuts at: 1678 2741 5334 1678 Size: 1063 2593 2531 Fragments arranged by size: 2593 2531 1063 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 3146 3178 4828 Size: 32 135 32 776 32 766 32 1650 Cuts at: 4828 4860 4902 4934 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1650 776 766 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3531 3777 669 Size: 988 281 1593 246 3079 Fragments arranged by size: 3079 1593 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6187 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3255 3786 3255 Size: 531 5656 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2214 3229 5301 5485 1305 Size: 909 1015 2072 184 2007 Fragments arranged by size: 2072 2007 1015 909 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4365 Fragments arranged by size: 4365 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2606 3150 4145 4504 4663 518 Size: 648 1440 544 995 359 159 2042 Fragments arranged by size: 2042 1440 995 648 544 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2936 3083 3603 4280 5428 5673 1606 Size: 423 907 147 520 677 1148 245 2120 Fragments arranged by size: 2120 1148 907 677 520 423 245 147 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4696 Fragments arranged by size: 4696 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 2170 2548 3358 3545 5090 1132 Size: 97 941 378 810 187 1545 2229 Fragments arranged by size: 2229 1545 941 810 378 187 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6187 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 2618 2699 3248 3326 1108 Size: 1510 81 549 78 3969 Fragments arranged by size: 3969 1510 549 81 78 PfoI T'CCnGG_A Cuts at: 2698 2698 Size: 6187 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4256 4288 5769 5801 4256 Size: 32 1481 32 4642 Fragments arranged by size: 4642 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2144 2945 2144 Size: 801 5386 PshAI GACnn'nnGTC Cuts at: 1613 2843 1613 Size: 1230 4957 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PstI C_TGCA'G Cuts at: 830 2371 3073 3539 830 Size: 1541 702 466 3478 Fragments arranged by size: 3478 1541 702 466 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 PvuII CAG'CTG Cuts at: 2029 2936 2029 Size: 907 5280 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 3067 4435 6147 3067 Size: 1368 1712 3107 Fragments arranged by size: 3107 1712 1368 SexAI A'CCwGG_T Cuts at: 2671 2671 Size: 6187 SfcI C'TryA_G Cuts at: 826 1045 2367 2994 3036 3069 3535 4670 5351 Size: 219 1322 627 42 33 466 1135 681 Cuts at: 5351 5542 826 Size: 191 1471 Fragments arranged by size: 1471 1322 1135 681 627 466 219 191 42 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 3514 1872 Size: 1642 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2718 2854 4291 5192 5454 820 Size: 197 926 775 136 1437 901 262 1553 Fragments arranged by size: 1553 1437 926 901 775 262 197 136 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 3050 3122 152 Size: 2898 72 3217 Fragments arranged by size: 3217 2898 72 SphI G_CATG'C Cuts at: 1132 2170 2548 3358 3545 1132 Size: 1038 378 810 187 3774 Fragments arranged by size: 3774 1038 810 378 187 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6187 StyI C'CwwG_G Cuts at: 513 1065 2004 2941 3626 513 Size: 552 939 937 685 3074 Fragments arranged by size: 3074 939 937 685 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2594 2606 4214 4231 4384 4569 5014 Size: 1255 1014 12 1608 17 153 185 445 Cuts at: 5014 325 Size: 1498 Fragments arranged by size: 1608 1498 1255 1014 445 185 153 17 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2858 3053 3065 Size: 274 80 33 51 1443 881 195 12 Cuts at: 3065 4433 6145 96 Size: 1368 1712 138 Fragments arranged by size: 1712 1443 1368 881 274 195 138 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6147 6179 6147 Size: 32 6155 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6187 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2948 2948 Size: 6187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 3512 1870 Size: 1642 4545 XmnI GAAnn'nnTTC Cuts at: 2256 4316 2256 Size: 2060 4127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI