Restriction map for
pFN21AB5205
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI KpnI KspI
MaeI MaeII MluI MroI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 1129 GGCGCC G[GA]^CG[CT]C
AflIII 920 ACATGT A^C[GA][CT]GT
AocI 943 CCTGAGG CC^T[ACGT]AGG
BalI 893 TGGCCA TGG^CCA
BbeI 1129 GGCGCC GGCGC^C
BbsI 651 GAAGAC GAAGAC(2/6)
BcgI 1443 CGAGGACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1602 CCGGG CC^[GC]GG
BglI 1516 GCCTCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 851 CTGGAG CTGGAG(16/14)
BpuAI 651 GAAGAC GAAGAC(2/6)
BsaBI 582 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1129 GGCGCC G[GA]^CG[CT]C
BsaI 1048 GAGACC GGTCTC(1/5)
BspHI 1178 TCATGA T^CATGA
BspLU11I 920 ACATGT A^CATGT
Bsu36I 943 CCTGAGG CC^T[ACGT]AGG
DraII 120 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 1152 GACAGAGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 943 CCTGAGG CC^T[ACGT]AGG
EcoO109I 120 GGGTCCC [GA]G^G[ACGT]CC[CT]
HaeII 1129 GGCGCC [GA]GCGC^[CT]
HapII 1602 CCGG C^CGG
Hin1I 1129 GGCGCC G[GA]^CG[CT]C
HincII 397 GTTAAC GT[CT]^[GA]AC
HindII 397 GTTAAC GT[CT]^[GA]AC
HpaI 397 GTTAAC GTT^AAC
HpaII 1602 CCGG C^CGG
KasI 1129 GGCGCC G^GCGCC
MamI 582 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 794 CAATTG C^AATTG
MluNI 893 TGGCCA TGG^CCA
MscI 893 TGGCCA TGG^CCA
MspI 1602 CCGG C^CGG
MunI 794 CAATTG C^AATTG
NarI 1129 GGCGCC GG^CGCC
NciI 1602 CCGGG CC^[GC]GG
PleI 767 GAGTC GAGTC(4/5)
PpuMI 120 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1178 TCATGA T^CATGA
ScaI 902 AGTACT AGT^ACT
SfoI 1129 GGCGCC GGC^GCC
SphI 1401 GCATGC GCATG^C
StuI 1319 AGGCCT AGG^CCT
XcmI 881 CCATTGGGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 238 GTAGAC GT^[AC][GT]AC
1429 GTAGAC
Asp700I 513 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
651 GAAGACTTTC
AspHI 489 GAGCTC G[AT]GC[AT]^C
727 GAGCTC
AvaI 613 CTCGAG C^[CT]CG[GA]G
730 CTCGAG
AvaII 121 GGTCC G^G[AT]CC
1160 GGTCC
BanI 277 GGCACC G^G[CT][GA]CC
1129 GGCGCC
BbvCI 97 GCTGAGG CCTCAGC(-5/-2)
262 GCTGAGG
BseRI 946 GAGGAG GAGGAG(10/8)
1147 GAGGAG
BsiHKAI 489 GAGCTC G[AT]GC[AT]^C
727 GAGCTC
BsiYI 126 CCGTGGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
879 CCCCATTGGGG
BslI 126 CCGTGGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
879 CCCCATTGGGG
BsmAI 1048 GAGAC GTCTC(1/5)
1150 GAGAC
BsmI 1002 GAATGC GAATGC(1/-1)
643 GCATTC
BsoBI 613 CTCGAG C^[CT]CG[GA]G
730 CTCGAG
BspMI 1256 ACCTGC ACCTGC(4/8)
318 GCAGGT
BsrDI 243 CATTGC GCAATG(2/0)
1449 CATTGC
BstYI 839 AGATCC [GA]^GATC[CT]
951 GGATCT
CfoI 1130 GCGC GCG^C
1504 GCGC
EarI 957 GAAGAG CTCTTC(1/4)
1045 GAAGAG
HhaI 1130 GCGC GCG^C
1504 GCGC
HinP1I 1130 GCGC G^CGC
1504 GCGC
Ksp632I 957 GAAGAG CTCTTC(1/4)
1045 GAAGAG
MflI 839 AGATCC [GA]^GATC[CT]
951 GGATCT
MseI 398 TTAA T^TAA
575 TTAA
MslI 282 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1233 CATTGAGATG
PaeR7I 613 CTCGAG C^TCGAG
730 CTCGAG
SacI 489 GAGCTC GAGCT^C
727 GAGCTC
Tru9I 398 TTAA T^TAA
575 TTAA
XhoI 613 CTCGAG C^TCGAG
730 CTCGAG
XhoII 839 AGATCC [GA]^GATC[CT]
951 GGATCT
XmnI 513 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
651 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BanII BbvI BmyI BsaJI BsmFI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I