(Circular) (Six-base) MAPSORT of: pFN21AB5205.seq Check: 9716 from: 1 to: 6334 pFN21AB5205 6334 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2314 2314 Size: 6334 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2244 3435 3677 1937 Size: 307 1191 242 4594 Fragments arranged by size: 4594 1191 307 242 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2339 2981 4397 5781 6049 1632 Size: 707 642 1416 1384 268 1917 Fragments arranged by size: 1917 1416 1384 707 642 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 2925 5233 6210 1203 Size: 1722 2308 977 1327 Fragments arranged by size: 2308 1722 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 5063 5063 Size: 6334 AleI CACnn'nnGTG Cuts at: 1166 2291 1166 Size: 1125 5209 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6334 AlwNI CAG_nnn'CTG Cuts at: 2088 3301 3524 4077 5649 2088 Size: 1213 223 553 1572 2773 Fragments arranged by size: 2773 1572 1213 553 223 ApaLI G'TGCA_C Cuts at: 4391 5547 4391 Size: 1156 5178 ApoI r'AATT_y Cuts at: 1493 3643 3869 4021 6130 1493 Size: 2150 226 152 2109 1697 Fragments arranged by size: 2150 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 2618 2735 3659 1870 Size: 73 675 117 924 4545 Fragments arranged by size: 4545 924 675 117 73 BamHI G'GATC_C Cuts at: 3664 4031 3664 Size: 367 5967 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2282 3134 3655 5110 618 Size: 325 217 627 495 852 521 1455 1842 Fragments arranged by size: 1842 1455 852 627 521 495 325 217 BanII G_rGCy'C Cuts at: 1391 2498 2617 2736 3403 3653 1391 Size: 1107 119 119 667 250 4072 Fragments arranged by size: 4072 1107 667 250 119 119 BbeI G_GCGC'C Cuts at: 3138 3138 Size: 6334 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2663 928 Size: 971 764 4599 Fragments arranged by size: 4599 971 764 BbvCI CC'TCA_GC Cuts at: 2103 2268 2103 Size: 165 6169 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3437 3471 4525 4559 1903 Size: 34 1500 34 1054 34 3678 Fragments arranged by size: 3678 1500 1054 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4226 5436 4226 Size: 1210 5124 BclI T'GATC_A Cuts at: 1548 1548 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3527 4945 136 Size: 107 122 71 631 605 1855 1418 1525 Fragments arranged by size: 1855 1525 1418 631 605 122 107 71 BglII A'GATC_T Cuts at: 6329 6329 Size: 6334 BlpI GC'TnA_GC Cuts at: 3751 3751 Size: 6334 Bme1580I G_kGCm'C Cuts at: 1790 4395 5551 1790 Size: 2605 1156 2573 Fragments arranged by size: 2605 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2899 4065 5023 Size: 416 53 83 399 24 1482 1166 958 Cuts at: 5023 442 Size: 1753 Fragments arranged by size: 1753 1482 1166 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2877 4994 1239 Size: 1638 2117 2579 Fragments arranged by size: 2579 2117 1638 Bpu10I CC'TnA_GC Cuts at: 2103 2268 2103 Size: 165 6169 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3259 4459 5324 5622 3259 Size: 1200 865 298 3971 Fragments arranged by size: 3971 1200 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3047 4997 882 Size: 617 1548 1950 2219 Fragments arranged by size: 2219 1950 1548 617 BsaAI yAC'GTr Cuts at: 493 1204 4125 493 Size: 711 2921 2702 Fragments arranged by size: 2921 2702 711 BsaBI GATnn'nnATC Cuts at: 2591 6328 2591 Size: 3737 2597 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3135 4141 4523 5158 Size: 53 83 186 936 1602 1006 382 635 Cuts at: 5158 275 Size: 1451 Fragments arranged by size: 1602 1451 1006 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4767 5165 5439 5586 5997 4767 Size: 398 274 147 411 5104 Fragments arranged by size: 5104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2850 2880 1178 Size: 30 165 30 1447 30 4632 Fragments arranged by size: 4632 1447 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2966 3167 1186 Size: 1780 201 4353 Fragments arranged by size: 4353 1780 201 BseYI C'CCAG_C Cuts at: 2064 2391 2511 2672 3028 5537 6033 2064 Size: 327 120 161 356 2509 496 2365 Fragments arranged by size: 2509 2365 496 356 327 161 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5761 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3784 4545 5573 1540 Size: 275 186 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2498 2736 4395 4480 5551 2498 Size: 238 1659 85 1071 3281 Fragments arranged by size: 3281 1659 1071 238 85 BsmI GAATG_Cn' Cuts at: 2648 3013 3845 3938 2648 Size: 365 832 93 5044 Fragments arranged by size: 5044 832 365 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2498 2617 2736 3403 3653 4395 4480 Size: 399 708 119 119 667 250 742 85 Cuts at: 4480 5551 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 708 667 399 250 119 119 85 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 3183 4223 5953 3183 Size: 1040 1730 3564 Fragments arranged by size: 3564 1730 1040 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2314 3270 3689 844 Size: 1470 956 419 3489 Fragments arranged by size: 3489 1470 956 419 BsrBI CCG'CTC Cuts at: 1394 1823 2489 3163 3576 4221 1394 Size: 429 666 674 413 645 3507 Fragments arranged by size: 3507 674 666 645 429 413 BsrDI GCAATG_nn' Cuts at: 65 1248 2247 3453 4829 5003 65 Size: 1183 999 1206 1376 174 1396 Fragments arranged by size: 1396 1376 1206 1183 999 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4978 5165 1700 Size: 110 3168 187 2869 Fragments arranged by size: 3168 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6334 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6334 BssSI C'ACGA_G Cuts at: 4394 5406 4394 Size: 1012 5322 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6334 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6334 BstXI CCAn_nnnn'nTGG Cuts at: 2395 2855 3244 3328 2395 Size: 460 389 84 5401 Fragments arranged by size: 5401 460 389 84 BstYI r'GATC_y Cuts at: 1972 2844 2956 3664 4031 4416 4433 5885 5971 Size: 872 112 708 367 385 17 1452 86 Cuts at: 5971 5983 6329 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 872 708 385 367 346 112 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2949 2949 Size: 6334 BtgI C'CryG_G Cuts at: 513 1065 2131 3127 3613 513 Size: 552 1066 996 486 3234 Fragments arranged by size: 3234 1066 996 552 486 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5595 Fragments arranged by size: 5595 683 56 BtsI GCAGTG_nn' Cuts at: 2556 3850 4644 4664 2556 Size: 1294 794 20 4226 Fragments arranged by size: 4226 1294 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3163 5341 809 Size: 507 1847 2178 1802 Fragments arranged by size: 2178 1847 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2898 2938 3622 Size: 54 1425 50 275 18 1068 40 684 Cuts at: 3622 3781 4670 6299 8 Size: 159 889 1629 43 Fragments arranged by size: 1629 1425 1068 889 684 275 159 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 3781 1537 Size: 275 1969 4090 Fragments arranged by size: 4090 1969 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2957 3045 4264 1353 Size: 1604 88 1219 3423 Fragments arranged by size: 3423 1604 1219 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4921 5295 5441 387 Size: 1064 138 3332 374 146 1280 Fragments arranged by size: 3332 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2496 2734 2496 Size: 238 6096 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2877 2981 4397 4994 5781 6049 Size: 393 707 538 104 1416 597 787 268 Cuts at: 6049 1239 Size: 1524 Fragments arranged by size: 1524 1416 787 707 597 538 393 268 104 EcoO109I rG'GnC_Cy Cuts at: 2126 2126 Size: 6334 EcoRI G'AATT_C Cuts at: 3643 4021 3643 Size: 378 5956 FspI TGC'GCA Cuts at: 4840 4840 Size: 6334 HaeII r_GCGC'y Cuts at: 1678 3138 5481 1678 Size: 1460 2343 2531 Fragments arranged by size: 2531 2343 1460 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2976 3008 3423 3455 4975 Size: 32 135 32 1404 32 415 32 1520 Cuts at: 4975 5007 5049 5081 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1520 1404 415 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2404 3678 3924 669 Size: 988 281 466 1274 246 3079 Fragments arranged by size: 3079 1274 988 466 281 246 HindIII A'AGCT_T Cuts at: 748 2144 2520 3471 748 Size: 1396 376 951 3611 Fragments arranged by size: 3611 1396 951 376 HpaI GTT'AAC Cuts at: 2404 3924 2404 Size: 1520 4814 KasI G'GCGC_C Cuts at: 3134 3134 Size: 6334 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 2799 3933 2799 Size: 1134 5200 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5448 5632 1305 Size: 4143 184 2007 Fragments arranged by size: 4143 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2900 10 Size: 54 1425 343 1068 3444 Fragments arranged by size: 3444 1425 1068 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2291 3242 4292 4651 4810 518 Size: 648 1125 951 1050 359 159 2042 Fragments arranged by size: 2042 1125 1050 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2395 2632 3301 3349 3750 4427 5575 5820 Size: 789 237 669 48 401 677 1148 245 Cuts at: 5820 1606 Size: 2120 Fragments arranged by size: 2120 1148 789 677 669 401 245 237 48 NarI GG'CG_CC Cuts at: 3135 3135 Size: 6334 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5782 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 2929 3114 3410 3692 5237 1132 Size: 97 1700 185 296 282 1545 2229 Fragments arranged by size: 2229 1700 1545 296 282 185 97 PasI CC'CwG_GG Cuts at: 1348 2122 3412 1348 Size: 774 1290 4270 Fragments arranged by size: 4270 1290 774 PciI A'CATG_T Cuts at: 2925 5233 2925 Size: 2308 4026 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6334 PfoI T'CCnGG_A Cuts at: 3286 3286 Size: 6334 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3560 3592 4403 4435 5916 5948 3560 Size: 32 811 32 1481 32 3946 Fragments arranged by size: 3946 1481 811 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2126 2126 Size: 6334 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PspXI vC'TCGA_Gb Cuts at: 2618 2735 2618 Size: 117 6217 PstI C_TGCA'G Cuts at: 830 2230 2311 2577 3686 830 Size: 1400 81 266 1109 3478 Fragments arranged by size: 3478 1400 1109 266 81 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 2395 2632 3301 3349 2395 Size: 237 669 48 5380 Fragments arranged by size: 5380 669 237 48 SacI G_AGCT'C Cuts at: 2498 2736 2498 Size: 238 6096 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SanDI GG'GwC_CC Cuts at: 2126 2126 Size: 6334 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 2909 4582 6294 2909 Size: 1673 1712 2949 Fragments arranged by size: 2949 1712 1673 SfcI C'TryA_G Cuts at: 826 1045 2226 2307 2573 3549 3682 4817 5498 Size: 219 1181 81 266 976 133 1135 681 Cuts at: 5498 5689 826 Size: 191 1471 Fragments arranged by size: 1471 1181 1135 976 681 266 219 191 133 81 SfoI GGC'GCC Cuts at: 3136 3136 Size: 6334 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 3661 1872 Size: 1789 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2618 2735 3274 4438 5339 5601 Size: 197 926 675 117 539 1164 901 262 Cuts at: 5601 820 Size: 1553 Fragments arranged by size: 1553 1164 926 901 675 539 262 197 117 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 3410 3692 1132 Size: 2278 282 3774 Fragments arranged by size: 3774 2278 282 SspI AAT'ATT Cuts at: 5 52 4258 5 Size: 47 4206 2081 Fragments arranged by size: 4206 2081 47 StuI AGG'CCT Cuts at: 1517 3326 1517 Size: 1809 4525 StyI C'CwwG_G Cuts at: 513 1065 2053 3099 3321 3327 3773 513 Size: 552 988 1046 222 6 446 3074 Fragments arranged by size: 3074 1046 988 552 446 222 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2264 2405 2918 4361 4378 4531 4716 Size: 1255 684 141 513 1443 17 153 185 Cuts at: 4716 5161 325 Size: 445 1498 Fragments arranged by size: 1498 1443 1255 684 513 445 185 153 141 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2907 4580 6292 Size: 274 80 33 51 1443 930 1673 1712 Cuts at: 6292 96 Size: 138 Fragments arranged by size: 1712 1673 1443 930 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2007 2039 6294 6326 2007 Size: 32 4255 32 2015 Fragments arranged by size: 4255 2015 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2893 2893 Size: 6334 XhoI C'TCGA_G Cuts at: 1943 2618 2735 1943 Size: 675 117 5542 Fragments arranged by size: 5542 675 117 XmaI C'CCGG_G Cuts at: 1870 3659 1870 Size: 1789 4545 XmnI GAAnn'nnTTC Cuts at: 2522 2660 4463 2522 Size: 138 1803 4393 Fragments arranged by size: 4393 1803 138 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI