Restriction map for
pFN21AB5184
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BbeI
BbrPI BbsI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FseI
HgaI Hin1I HpaI KasI KpnI
KspI MluI MluNI MroI MscI
MseI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspI SwaI Tru9I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 252 GTCTAC GT^[AC][GT]AC
Aor51HI 902 AGCGCT AGC^GCT
AspHI 1499 GAGCTC G[AT]GC[AT]^C
AviII 1184 TGCGCA TGC^GCA
BbvCI 1459 GCTGAGG CCTCAGC(-5/-2)
BclI 573 TGATCA T^GATCA
BsaBI 891 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1499 GAGCTC G[AT]GC[AT]^C
Bsp1407I 1034 TGTACA T^GTACA
BspHI 1139 TCATGA T^CATGA
BsrGI 1034 TGTACA T^GTACA
BstXI 1623 CCAAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 603 TGGCCG [CT]^GGCC[GA]
Eco47III 902 AGCGCT AGC^GCT
EcoRV 1564 GATATC GAT^ATC
FbaI 573 TGATCA T^GATCA
FspI 1184 TGCGCA TGC^GCA
HaeII 902 AGCGCT [GA]GCGC^[CT]
HincII 90 GTTGAC GT[CT]^[GA]AC
HindII 90 GTTGAC GT[CT]^[GA]AC
HindIII 50 AAGCTT A^AGCTT
MaeII 1232 ACGT A^CGT
MamI 891 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1003 CAATTG C^AATTG
MunI 1003 CAATTG C^AATTG
NspI 911 ACATGC [GA]CATG^[CT]
RcaI 1139 TCATGA T^CATGA
SacI 1499 GAGCTC GAGCT^C
SapI 1746 GCTCTTC GCTCTTC(1/4)
ScaI 1654 AGTACT AGT^ACT
SfiI 630 GGCCTCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1369 CTCAAG C^T[CT][GA]AG
SpeI 1657 ACTAGT A^CTAGT
SspBI 1034 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 82 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
277 CAGGTTCTG
AvaI 224 CTCGGG C^[CT]CG[GA]G
818 CTCGGG
BamHI 1398 GGATCC G^GATCC
1589 GGATCC
BcgI 15 CGAGAGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1511 GCATTGGGCTCG
BciVI 498 GGATAC GTATCC(6/5)
1208 GGATAC
BpmI 784 CTCCAG CTGGAG(16/14)
145 CTGGAG
BsmI 327 GAATGC GAATGC(1/-1)
1530 GAATGC
BsoBI 224 CTCGGG C^[CT]CG[GA]G
818 CTCGGG
BspMI 234 ACCTGC ACCTGC(4/8)
25 GCAGGT
BstYI 1398 GGATCC [GA]^GATC[CT]
1589 GGATCC
EarI 1747 CTCTTC CTCTTC(1/4)
556 GAAGAG
Ksp632I 1747 CTCTTC CTCTTC(1/4)
556 GAAGAG
MaeIII 97 GTGAC ^GT[ACGT]AC
346 GTGAC
MflI 1398 GGATCC [GA]^GATC[CT]
1589 GGATCC
PpuMI 113 AGGTCCC [GA]G^G[AT]CC[CT]
657 GGGTCCC
PstI 1454 CTGCAG CTGCA^G
1466 CTGCAG
SfcI 1454 CTGCAG C^T[GA][CT]AG
1466 CTGCAG
StuI 131 AGGCCT AGG^CCT
245 AGGCCT
TaqI 73 TCGA T^CGA
1503 TCGA
Tsp45I 97 GTGAC ^GT[GC]AC
346 GTGAC
Tsp509I 1004 AATT ^AATT
1014 AATT
TthHB8I 73 TCGA T^CGA
1503 TCGA
XhoII 1398 GGATCC [GA]^GATC[CT]
1589 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BsaI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI TseI TspRI
Van91I