(Circular) (Six-base) MAPSORT of: pFN21AB5184.seq Check: 8098 from: 1 to: 6523 pFN21AB5184 6523 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2248 2248 Size: 6523 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4333 278 Size: 53 83 186 936 2797 2468 Fragments arranged by size: 2797 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2258 3866 1937 Size: 321 1608 4594 Fragments arranged by size: 4594 1608 321 Acc65I G'GTAC_C Cuts at: 3844 3844 Size: 6523 AclI AA'CG_TT Cuts at: 1563 4650 5023 1563 Size: 3087 373 3063 Fragments arranged by size: 3087 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2043 2073 2829 2942 3498 3694 4586 5970 Size: 411 30 756 113 556 196 892 1384 Cuts at: 5970 6238 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 892 756 556 411 268 196 113 30 AfeI AGC'GCT Cuts at: 2909 2909 Size: 6523 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6326 AflIII A'CryG_T Cuts at: 1203 5422 6399 1203 Size: 4219 977 1327 Fragments arranged by size: 4219 1327 977 AgeI A'CCGG_T Cuts at: 5354 5354 Size: 6523 AhdI GACnn_n'nnGTC Cuts at: 5252 5252 Size: 6523 AleI CACnn'nnGTG Cuts at: 1166 2948 1166 Size: 1782 4741 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6523 AlwNI CAG_nnn'CTG Cuts at: 2092 2287 4266 5838 2092 Size: 195 1979 1572 2777 Fragments arranged by size: 2777 1979 1572 195 ApaI G_GGCC'C Cuts at: 2683 2719 3549 2683 Size: 36 830 5657 Fragments arranged by size: 5657 830 36 ApaLI G'TGCA_C Cuts at: 4580 5736 4580 Size: 1156 5367 ApoI r'AATT_y Cuts at: 1493 3832 4058 4210 6319 1493 Size: 2339 226 152 2109 1697 Fragments arranged by size: 2339 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5077 160 Size: 872 4045 1606 Fragments arranged by size: 4045 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 AvaI C'yCGr_G Cuts at: 1870 1943 2229 2823 3848 1870 Size: 73 286 594 1025 4545 Fragments arranged by size: 4545 1025 594 286 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2268 2301 2388 2421 2268 Size: 33 87 33 6370 Fragments arranged by size: 6370 87 33 33 BamHI G'GATC_C Cuts at: 3403 3594 3853 4220 3403 Size: 191 259 367 5706 Fragments arranged by size: 5706 367 259 191 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2236 3653 3719 3776 3800 Size: 325 217 627 449 1417 66 57 24 Cuts at: 3800 3844 5299 618 Size: 44 1455 1842 Fragments arranged by size: 1842 1455 1417 627 449 325 217 66 57 44 24 BanII G_rGCy'C Cuts at: 1391 2683 2719 3066 3508 3525 3549 3753 3842 Size: 1292 36 347 442 17 24 204 89 Cuts at: 3842 1391 Size: 4072 Fragments arranged by size: 4072 1292 442 347 204 89 36 24 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5552 BbvCI CC'TCA_GC Cuts at: 3465 3465 Size: 6523 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2009 2043 3505 3539 4714 4748 1903 Size: 34 72 34 1462 34 1175 34 3678 Fragments arranged by size: 3678 1462 1175 72 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2497 3207 4415 5625 2497 Size: 710 1208 1210 3395 Fragments arranged by size: 3395 1210 1208 710 BclI T'GATC_A Cuts at: 1548 2578 1548 Size: 1030 5493 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2144 2249 2642 Size: 107 122 71 631 605 472 105 393 Cuts at: 2642 2651 2678 2687 5134 136 Size: 9 27 9 2447 1525 Fragments arranged by size: 2447 1525 631 605 472 393 122 107 105 71 27 9 9 BglII A'GATC_T Cuts at: 6518 6518 Size: 6523 BlpI GC'TnA_GC Cuts at: 3940 3940 Size: 6523 Bme1580I G_kGCm'C Cuts at: 1790 2683 2719 3549 4584 5740 1790 Size: 893 36 830 1035 1156 2573 Fragments arranged by size: 2573 1156 1035 893 830 36 BmgBI CAC'GTC Cuts at: 1318 3238 1318 Size: 1920 4603 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2479 3092 3565 Size: 416 53 83 399 24 1062 613 473 Cuts at: 3565 4254 5212 442 Size: 689 958 1753 Fragments arranged by size: 1753 1062 958 689 613 473 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6523 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2747 2747 Size: 6523 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2774 5183 1239 Size: 932 603 2409 2579 Fragments arranged by size: 2579 2409 932 603 Bpu10I CC'TnA_GC Cuts at: 2418 2463 3465 2418 Size: 45 1002 5476 Fragments arranged by size: 5476 1002 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3359 4648 5513 5811 3359 Size: 1289 865 298 4071 Fragments arranged by size: 4071 1289 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2161 2179 2197 2215 2363 2623 3347 Size: 617 662 18 18 18 148 260 724 Cuts at: 3347 5186 882 Size: 1839 2219 Fragments arranged by size: 2219 1839 724 662 617 260 148 18 18 18 BsaAI yAC'GTr Cuts at: 493 1204 4314 493 Size: 711 3110 2702 Fragments arranged by size: 3110 2702 711 BsaBI GATnn'nnATC Cuts at: 2900 6517 2900 Size: 3617 2906 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4330 4712 5347 275 Size: 53 83 186 936 2797 382 635 1451 Fragments arranged by size: 2797 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4956 5354 5628 5775 6186 4956 Size: 398 274 147 411 5293 Fragments arranged by size: 5293 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6298 Fragments arranged by size: 6298 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6523 BseYI C'CCAG_C Cuts at: 2449 3109 5726 6222 2449 Size: 660 2617 496 2750 Fragments arranged by size: 2750 2617 660 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2049 2418 2496 1163 Size: 573 313 369 78 5190 Fragments arranged by size: 5190 573 369 313 78 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3973 4734 5762 1540 Size: 275 186 1972 761 1028 2301 Fragments arranged by size: 2301 1972 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3508 4584 4669 5740 3508 Size: 1076 85 1071 4291 Fragments arranged by size: 4291 1076 1071 85 BsmI GAATG_Cn' Cuts at: 2338 3541 4034 4127 2338 Size: 1203 493 93 4734 Fragments arranged by size: 4734 1203 493 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2683 2719 3066 3508 3525 3549 3753 Size: 399 893 36 347 442 17 24 204 Cuts at: 3753 3842 4584 4669 5740 1391 Size: 89 742 85 1071 2174 Fragments arranged by size: 2174 1071 893 742 442 399 347 204 89 85 36 24 17 BspEI T'CCGG_A Cuts at: 6186 6186 Size: 6523 BspHI T'CATG_A Cuts at: 3144 4412 6142 3144 Size: 1268 1730 3525 Fragments arranged by size: 3525 1730 1268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2021 2248 3878 844 Size: 1177 227 1630 3489 Fragments arranged by size: 3489 1630 1177 227 BsrBI CCG'CTC Cuts at: 1394 1823 4410 1394 Size: 429 2587 3507 Fragments arranged by size: 3507 2587 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5018 5192 65 Size: 1183 3770 174 1396 Fragments arranged by size: 3770 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5167 5354 1700 Size: 110 3357 187 2869 Fragments arranged by size: 3357 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3039 96 Size: 2943 3580 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6523 BssSI C'ACGA_G Cuts at: 4583 5595 4583 Size: 1012 5511 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6523 BstBI TT'CG_AA Cuts at: 5327 5327 Size: 6523 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6523 BstXI CCAn_nnnn'nTGG Cuts at: 3635 3635 Size: 6523 BstYI r'GATC_y Cuts at: 1972 3403 3594 3853 4220 4605 4622 6074 6160 Size: 1431 191 259 367 385 17 1452 86 Cuts at: 6160 6172 6518 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1431 385 367 346 259 191 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5971 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3452 487 Size: 683 56 2226 3558 Fragments arranged by size: 3558 2226 683 56 BtsI GCAGTG_nn' Cuts at: 2581 3646 4039 4833 4853 2581 Size: 1065 393 794 20 4251 Fragments arranged by size: 4251 1065 794 393 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6523 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6488 DraI TTT'AAA Cuts at: 3827 3827 Size: 6523 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5530 809 Size: 507 4214 1802 Fragments arranged by size: 4214 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2608 3970 4859 Size: 54 1425 50 275 18 778 1362 889 Cuts at: 4859 6488 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1362 889 778 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3970 1537 Size: 275 2158 4090 Fragments arranged by size: 4090 2158 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2556 3758 4453 1353 Size: 1203 1202 695 3423 Fragments arranged by size: 3423 1203 1202 695 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5110 5484 5630 387 Size: 1064 138 3521 374 146 1280 Fragments arranged by size: 3521 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3506 3506 Size: 6523 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2073 2171 2774 2829 2942 3498 Size: 393 411 30 98 603 55 113 556 Cuts at: 3498 3694 4586 5183 5970 6238 1239 Size: 196 892 597 787 268 1524 Fragments arranged by size: 1524 892 787 603 597 556 411 393 268 196 113 98 55 30 EcoO109I rG'GnC_Cy Cuts at: 2119 2446 2663 3334 3546 2119 Size: 327 217 671 212 5096 Fragments arranged by size: 5096 671 327 217 212 EcoRI G'AATT_C Cuts at: 3832 4210 3832 Size: 378 6145 EcoRV GAT'ATC Cuts at: 3571 3571 Size: 6523 FspI TGC'GCA Cuts at: 3191 5029 3191 Size: 1838 4685 HaeII r_GCGC'y Cuts at: 1678 2911 5670 1678 Size: 1233 2759 2531 Fragments arranged by size: 2759 2531 1233 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2074 2092 2106 3490 Size: 32 135 32 488 14 18 14 1384 Cuts at: 3490 3522 3721 3753 5164 5196 5238 5270 1373 Size: 32 199 32 1411 32 42 32 2626 Fragments arranged by size: 2626 1411 1384 488 199 135 42 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 2097 3867 4113 669 Size: 988 281 159 1770 246 3079 Fragments arranged by size: 3079 1770 988 281 246 159 HindIII A'AGCT_T Cuts at: 748 2055 748 Size: 1307 5216 HpaI GTT'AAC Cuts at: 4113 4113 Size: 6523 KpnI G_GTAC'C Cuts at: 3848 3848 Size: 6523 MfeI C'AATT_G Cuts at: 3008 4122 3008 Size: 1114 5409 MluI A'CGCG_T Cuts at: 6399 6399 Size: 6523 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5637 5821 1305 Size: 4332 184 2007 Fragments arranged by size: 4332 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4701 Fragments arranged by size: 4701 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2553 2948 3143 4481 4840 4999 518 Size: 648 1387 395 195 1338 359 159 2042 Fragments arranged by size: 2042 1387 1338 648 395 359 195 159 MspA1I CmG'CkG Cuts at: 1606 2269 2294 2315 2480 3464 3476 3939 4616 Size: 663 25 21 165 984 12 463 677 Cuts at: 4616 5764 6009 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 984 677 663 463 245 165 25 21 12 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5971 NdeI CA'TA_TG Cuts at: 387 387 Size: 6523 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6523 NotI GC'GGCC_GC Cuts at: 3970 3970 Size: 6523 NspI r_CATG'y Cuts at: 1132 1229 2920 3881 5426 1132 Size: 97 1691 961 1545 2229 Fragments arranged by size: 2229 1691 1545 961 97 PasI CC'CwG_GG Cuts at: 1348 3209 3579 1348 Size: 1861 370 4292 Fragments arranged by size: 4292 1861 370 PciI A'CATG_T Cuts at: 5422 5422 Size: 6523 PflMI CCAn_nnn'nTGG Cuts at: 1108 2113 2287 3414 1108 Size: 1005 174 1127 4217 Fragments arranged by size: 4217 1127 1005 174 PmeI GTTT'AAAC Cuts at: 3827 3827 Size: 6523 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4592 4624 6105 6137 4592 Size: 32 1481 32 4978 Fragments arranged by size: 4978 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2663 2119 Size: 544 5979 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6523 PsiI TTA'TAA Cuts at: 4093 4093 Size: 6523 PspOMI G'GGCC_C Cuts at: 2679 2715 3545 2679 Size: 36 830 5657 Fragments arranged by size: 5657 830 36 PstI C_TGCA'G Cuts at: 830 3463 3475 3875 830 Size: 2633 12 400 3478 Fragments arranged by size: 3478 2633 400 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6523 PvuII CAG'CTG Cuts at: 2269 2294 2315 2480 3464 3476 2269 Size: 25 21 165 984 12 5316 Fragments arranged by size: 5316 984 165 25 21 12 SacI G_AGCT'C Cuts at: 3508 3508 Size: 6523 SalI G'TCGA_C Cuts at: 1936 3865 1936 Size: 1929 4594 SanDI GG'GwC_CC Cuts at: 2663 2663 Size: 6523 SapI GCTCTTCn'nnn_ Cuts at: 3758 3758 Size: 6523 SbfI CC_TGCA'GG Cuts at: 3875 3875 Size: 6523 ScaI AGT'ACT Cuts at: 3661 4771 6483 3661 Size: 1110 1712 3701 Fragments arranged by size: 3701 1712 1110 SfcI C'TryA_G Cuts at: 826 1045 3459 3471 3871 5006 5687 5878 826 Size: 219 2414 12 400 1135 681 191 1471 Fragments arranged by size: 2414 1471 1135 681 400 219 191 12 SfiI GGCCn_nnn'nGGCC Cuts at: 2642 2642 Size: 6523 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6523 SmaI CCC'GGG Cuts at: 1872 3850 1872 Size: 1978 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3374 4627 5528 5790 820 Size: 197 926 1431 1253 901 262 1553 Fragments arranged by size: 1553 1431 1253 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4314 493 Size: 3821 2702 SpeI A'CTAG_T Cuts at: 152 3662 152 Size: 3510 3013 SphI G_CATG'C Cuts at: 1132 3881 1132 Size: 2749 3774 SspI AAT'ATT Cuts at: 5 52 4447 5 Size: 47 4395 2081 Fragments arranged by size: 4395 2081 47 StuI AGG'CCT Cuts at: 1517 2138 2252 1517 Size: 621 114 5788 Fragments arranged by size: 5788 621 114 StyI C'CwwG_G Cuts at: 513 1065 2514 3408 3483 3763 3962 513 Size: 552 1449 894 75 280 199 3074 Fragments arranged by size: 3074 1449 894 552 280 199 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2261 3118 3147 4550 4567 4720 4905 Size: 1255 681 857 29 1403 17 153 185 Cuts at: 4905 5350 325 Size: 445 1498 Fragments arranged by size: 1498 1403 1255 857 681 445 185 153 29 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3039 3659 4769 Size: 274 80 33 51 1443 1062 620 1110 Cuts at: 4769 6481 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1110 1062 620 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6483 6515 6483 Size: 32 6491 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6523 XbaI T'CTAG_A Cuts at: 3859 3859 Size: 6523 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6523 XmaI C'CCGG_G Cuts at: 1870 3848 1870 Size: 1978 4545 XmnI GAAnn'nnTTC Cuts at: 4652 4652 Size: 6523 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4331 276 Size: 53 83 186 936 2797 2468 Fragments arranged by size: 2797 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI XcmI