Restriction map for
pFN21AB5184
(Linear) (Six-base) MAPSORT of: KIBB5184.seq Check: 1980 from: 1 to: 1818
KIBB5184.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 243 1818
Size: 243 1575
AccI GT'mk_AC
Cuts at: 0 253 1818
Size: 253 1565
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 68 824 937 1493 1689 1818
Size: 38 30 756 113 556 196 129
Fragments arranged by size:
756 556 196 129 113 38 30
AfeI AGC'GCT
Cuts at: 0 904 1818
Size: 904 914
AleI CACnn'nnGTG
Cuts at: 0 943 1818
Size: 943 875
AlwNI CAG_nnn'CTG
Cuts at: 0 87 282 1818
Size: 87 195 1536
Fragments arranged by size:
1536 195 87
ApaI G_GGCC'C
Cuts at: 0 678 714 1544 1818
Size: 678 36 830 274
Fragments arranged by size:
830 678 274 36
AvaI C'yCGr_G
Cuts at: 0 224 818 1818
Size: 224 594 1000
Fragments arranged by size:
1000 594 224
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 263 296 383 416 1818
Size: 263 33 87 33 1402
Fragments arranged by size:
1402 263 87 33 33
BamHI G'GATC_C
Cuts at: 0 1398 1589 1818
Size: 1398 191 229
Fragments arranged by size:
1398 229 191
BanI G'GyrC_C
Cuts at: 0 231 1648 1714 1771 1795 1818
Size: 231 1417 66 57 24 23
Fragments arranged by size:
1417 231 66 57 24 23
BanII G_rGCy'C
Cuts at: 0 678 714 1061 1503 1520 1544 1748 1818
Size: 678 36 347 442 17 24 204 70
Fragments arranged by size:
678 442 347 204 70 36 24 17
BbvCI CC'TCA_GC
Cuts at: 0 1460 1818
Size: 1460 358
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4 38 1500 1534 1818
Size: 4 34 1462 34 284
Fragments arranged by size:
1462 284 34 34 4
BciVI GTATCCnnnnn_n'
Cuts at: 0 492 1202 1818
Size: 492 710 616
Fragments arranged by size:
710 616 492
BclI T'GATC_A
Cuts at: 0 573 1818
Size: 573 1245
BglI GCCn_nnn'nGGC
Cuts at: 0 139 244 637 646 673 682 1818
Size: 139 105 393 9 27 9 1136
Fragments arranged by size:
1136 393 139 105 27 9 9
Bme1580I G_kGCm'C
Cuts at: 0 678 714 1544 1818
Size: 678 36 830 274
Fragments arranged by size:
830 678 274 36
BmgBI CAC'GTC
Cuts at: 0 1233 1818
Size: 1233 585
BmrI ACTGGGnnnn_n'
Cuts at: 0 474 1087 1560 1818
Size: 474 613 473 258
Fragments arranged by size:
613 474 473 258
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 742 1818
Size: 742 1076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 769 1818
Size: 166 603 1049
Fragments arranged by size:
1049 603 166
Bpu10I CC'TnA_GC
Cuts at: 0 413 458 1460 1818
Size: 413 45 1002 358
Fragments arranged by size:
1002 413 358 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1354 1818
Size: 1354 464
BsaI GGTCTCn'nnnn_
Cuts at: 0 156 174 192 210 358 618 1342 1818
Size: 156 18 18 18 148 260 724 476
Fragments arranged by size:
724 476 260 156 148 18 18 18
BsaBI GATnn'nnATC
Cuts at: 0 895 1818
Size: 895 923
BseYI C'CCAG_C
Cuts at: 0 444 1104 1818
Size: 444 660 714
Fragments arranged by size:
714 660 444
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 413 491 1818
Size: 44 369 78 1327
Fragments arranged by size:
1327 369 78 44
BsiHKAI G_wGCw'C
Cuts at: 0 1503 1818
Size: 1503 315
BsmI GAATG_Cn'
Cuts at: 0 333 1536 1818
Size: 333 1203 282
Fragments arranged by size:
1203 333 282
Bsp1286I G_dGCh'C
Cuts at: 0 678 714 1061 1503 1520 1544 1748 1818
Size: 678 36 347 442 17 24 204 70
Fragments arranged by size:
678 442 347 204 70 36 24 17
BspHI T'CATG_A
Cuts at: 0 1139 1818
Size: 1139 679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 16 243 1818
Size: 16 227 1575
Fragments arranged by size:
1575 227 16
BsrGI T'GTAC_A
Cuts at: 0 1034 1818
Size: 1034 784
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1630 1818
Size: 1630 188
BstYI r'GATC_y
Cuts at: 0 1398 1589 1818
Size: 1398 191 229
Fragments arranged by size:
1398 229 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1447 1818
Size: 1447 371
BtsI GCAGTG_nn'
Cuts at: 0 576 1641 1818
Size: 576 1065 177
Fragments arranged by size:
1065 576 177
EaeI y'GGCC_r
Cuts at: 0 603 1818
Size: 603 1215
EarI CTCTTCn'nnn_
Cuts at: 0 551 1753 1818
Size: 551 1202 65
Fragments arranged by size:
1202 551 65
EcoICRI GAG'CTC
Cuts at: 0 1501 1818
Size: 1501 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 68 166 769 824 937 1493 1689
Size: 38 30 98 603 55 113 556 196
Cuts at: 1689 1818
Size: 129
Fragments arranged by size:
603 556 196 129 113 98 55 38
30
EcoO109I rG'GnC_Cy
Cuts at: 0 114 441 658 1329 1541 1818
Size: 114 327 217 671 212 277
Fragments arranged by size:
671 327 277 217 212 114
EcoRV GAT'ATC
Cuts at: 0 1566 1818
Size: 1566 252
FspI TGC'GCA
Cuts at: 0 1186 1818
Size: 1186 632
HaeII r_GCGC'y
Cuts at: 0 906 1818
Size: 906 912
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 69 87 101 1485 1517 1716 1748
Size: 55 14 18 14 1384 32 199 32
Cuts at: 1748 1818
Size: 70
Fragments arranged by size:
1384 199 70 55 32 32 18 14
14
HincII GTy'rAC
Cuts at: 0 92 1818
Size: 92 1726
HindIII A'AGCT_T
Cuts at: 0 50 1818
Size: 50 1768
MfeI C'AATT_G
Cuts at: 0 1003 1818
Size: 1003 815
MslI CAynn'nnrTG
Cuts at: 0 548 943 1138 1818
Size: 548 395 195 680
Fragments arranged by size:
680 548 395 195
MspA1I CmG'CkG
Cuts at: 0 264 289 310 475 1459 1471 1818
Size: 264 25 21 165 984 12 347
Fragments arranged by size:
984 347 264 165 25 21 12
NspI r_CATG'y
Cuts at: 0 915 1818
Size: 915 903
PasI CC'CwG_GG
Cuts at: 0 1204 1574 1818
Size: 1204 370 244
Fragments arranged by size:
1204 370 244
PflMI CCAn_nnn'nTGG
Cuts at: 0 108 282 1409 1818
Size: 108 174 1127 409
Fragments arranged by size:
1127 409 174 108
PpuMI rG'GwC_Cy
Cuts at: 0 114 658 1818
Size: 114 544 1160
Fragments arranged by size:
1160 544 114
PspOMI G'GGCC_C
Cuts at: 0 674 710 1540 1818
Size: 674 36 830 278
Fragments arranged by size:
830 674 278 36
PstI C_TGCA'G
Cuts at: 0 1458 1470 1818
Size: 1458 12 348
Fragments arranged by size:
1458 348 12
PvuII CAG'CTG
Cuts at: 0 264 289 310 475 1459 1471 1818
Size: 264 25 21 165 984 12 347
Fragments arranged by size:
984 347 264 165 25 21 12
SacI G_AGCT'C
Cuts at: 0 1503 1818
Size: 1503 315
SanDI GG'GwC_CC
Cuts at: 0 658 1818
Size: 658 1160
SapI GCTCTTCn'nnn_
Cuts at: 0 1753 1818
Size: 1753 65
ScaI AGT'ACT
Cuts at: 0 1656 1818
Size: 1656 162
SfcI C'TryA_G
Cuts at: 0 1454 1466 1818
Size: 1454 12 352
Fragments arranged by size:
1454 352 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 637 1818
Size: 637 1181
SmlI C'TyrA_G
Cuts at: 0 1369 1818
Size: 1369 449
SpeI A'CTAG_T
Cuts at: 0 1657 1818
Size: 1657 161
StuI AGG'CCT
Cuts at: 0 133 247 1818
Size: 133 114 1571
Fragments arranged by size:
1571 133 114
StyI C'CwwG_G
Cuts at: 0 509 1403 1478 1758 1818
Size: 509 894 75 280 60
Fragments arranged by size:
894 509 280 75 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 256 1113 1142 1818
Size: 256 857 29 676
Fragments arranged by size:
857 676 256 29
TatI w'GTAC_w
Cuts at: 0 1034 1654 1818
Size: 1034 620 164
Fragments arranged by size:
1034 620 164
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlwNI ApaI AvaI
BaeI BamHI BanI BanII BbvCI BcgI BciVI BclI
BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrGI BstXI BstYI BtgZI BtsI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRV FspI HaeII Hin4I HincII
HindIII MfeI MslI MspA1I NspI PasI PflMI PpuMI
PspOMI PstI PvuII SacI SanDI SapI ScaI SfcI
SfiI SmlI SpeI StuI StyI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BbeI
BbsI BfrBI BglII BlpI BmtI BsaAI BsaHI BsaWI
BsaXI BseRI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRI FalI FseI FspAI HpaI KasI KpnI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SbfI SexAI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI