Restriction map for
pFN21AB5164
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BbeI BcgI BciVI BclI
BfaI BfrI BlnI BsaBI BsaHI
BsaI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MseI MslI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tru9I Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 95 CGCG CG^CG
AccIII 369 TCCGGA T^CCGGA
AcsI 234 GAATTT [GA]^AATT[CT]
AocI 1113 CCTCAGG CC^T[ACGT]AGG
ApoI 234 GAATTT [GA]^AATT[CT]
Asp700I 409 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 721 TGGCCA TGG^CCA
BamHI 20 GGATCC G^GATCC
BbrPI 1107 CACGTG CAC^GTG
BbvCI 502 CCTCAGC CCTCAGC(-5/-2)
BglII 981 AGATCT A^GATCT
BlpI 792 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 792 GCTGAGC GC^T[ACGT]AGC
BseAI 369 TCCGGA T^CCGGA
BsgI 1177 CTGCAC GTGCAG(16/14)
BsmAI 701 GAGAC GTCTC(1/5)
BspEI 369 TCCGGA T^CCGGA
BsrBI 240 CCGCTC CCGCTC(-3/-3)
BssSI 1084 CTCGTG CACGAG(-5/-1)
BstUI 95 CGCG CG^CG
BstXI 1263 CCAGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1113 CCTCAGG CC^T[ACGT]AGG
CelII 792 GCTGAGC GC^T[ACGT]AGC
CfoI 96 GCGC GCG^C
EaeI 721 TGGCCA [CT]^GGCC[GA]
Eco81I 1113 CCTCAGG CC^T[ACGT]AGG
HhaI 96 GCGC GCG^C
HinP1I 96 GCGC G^CGC
MluNI 721 TGGCCA TGG^CCA
MroI 369 TCCGGA T^CCGGA
MscI 721 TGGCCA TGG^CCA
MvnI 95 CGCG CG^CG
NdeI 188 CATATG CA^TATG
PleI 1048 GAGTC GAGTC(4/5)
PmaCI 1107 CACGTG CAC^GTG
PmlI 1107 CACGTG CAC^GTG
PstI 1234 CTGCAG CTGCA^G
PvuII 43 CAGCTG CAG^CTG
SbfI 1233 CCTGCAGG CCTGCA^GG
ScaI 391 AGTACT AGT^ACT
Sse8387I 1233 CCTGCAGG CCTGCA^GG
TaqI 611 TCGA T^CGA
TfiI 155 GAATC G^A[AT]TC
Tsp509I 235 AATT ^AATT
TthHB8I 611 TCGA T^CGA
XmnI 409 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 392 GTAC GT^AC
1221 GTAC
AvaI 196 CCCGGG C^[CT]CG[GA]G
1136 CCCGGG
BanI 588 GGTGCC G^G[CT][GA]CC
1147 GGTGCC
BbsI 409 GAAGAC GAAGAC(2/6)
643 GTCTTC
BglI 665 GCCCGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
714 GCCTGGATGGC
BpmI 61 CTGGAG CTGGAG(16/14)
1159 CTGGAG
BpuAI 409 GAAGAC GAAGAC(2/6)
643 GTCTTC
BsaAI 778 TACGTG [CT]AC^GT[GA]
1107 CACGTG
BsaWI 284 ACCGGA [AT]^CCGG[AT]
369 TCCGGA
BseRI 1152 CTCCTC GAGGAG(10/8)
889 GAGGAG
BsoBI 196 CCCGGG C^[CT]CG[GA]G
1136 CCCGGG
DsaI 16 CCACGG C^C[GA][CT]GG
1069 CCGTGG
EarI 228 GAAGAG CTCTTC(1/4)
598 GAAGAG
EcoNI 123 CCTACGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
224 CCTGGAAGAGG
FauI 825 GCGGG CCCGC(4/6)
908 GCGGG
HgaI 708 GACGC GACGC(5/10)
1010 GACGC
HinfI 155 GAATC G^A[ACGT]TC
1048 GAGTC
Ksp632I 228 GAAGAG CTCTTC(1/4)
598 GAAGAG
MaeII 779 ACGT A^CGT
1108 ACGT
NspI 746 GCATGT [GA]CATG^[CT]
786 GCATGT
PflMI 736 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
849 CCAGCGGCTGG
RsaI 392 GTAC GT^AC
1221 GTAC
SfaNI 133 GATGC GCATC(5/9)
104 GCATC
SfcI 482 CTGTAG C^T[GA][CT]AG
1234 CTGCAG
SmaI 196 CCCGGG CCC^GGG
1136 CCCGGG
StuI 164 AGGCCT AGG^CCT
1226 AGGCCT
Tsp45I 616 GTGAC ^GT[GC]AC
727 GTCAC
Van91I 736 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
849 CCAGCGGCTGG
XmaCI 196 CCCGGG C^CCGGG
1136 CCCGGG
XmaI 196 CCCGGG C^CCGGG
1136 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
Sau3AI Sau96I ScrFI StyI TseI
TspRI XhoII