(Circular) (Six-base) MAPSORT of: pFN21AB5164.seq Check: 9575 from: 1 to: 5986 pFN21AB5164 5986 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 4049 5433 5701 1632 Size: 968 1449 1384 268 1917 Fragments arranged by size: 1917 1449 1384 968 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 4885 5862 1203 Size: 3682 977 1327 Fragments arranged by size: 3682 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AlwNI CAG_nnn'CTG Cuts at: 2515 2747 2860 3729 5301 2515 Size: 232 113 869 1572 3200 Fragments arranged by size: 3200 1572 869 232 113 ApaI G_GGCC'C Cuts at: 2565 2854 2976 3275 2565 Size: 289 122 299 5276 Fragments arranged by size: 5276 299 289 122 ApaLI G'TGCA_C Cuts at: 4043 5199 4043 Size: 1156 4830 ApoI r'AATT_y Cuts at: 1493 2239 3295 3521 3673 5782 1493 Size: 746 1056 226 152 2109 1697 Fragments arranged by size: 2109 1697 1056 746 226 152 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 2201 3141 3311 1870 Size: 73 258 940 170 4545 Fragments arranged by size: 4545 940 258 170 73 BamHI G'GATC_C Cuts at: 2025 3316 3683 2025 Size: 1291 367 4328 Fragments arranged by size: 4328 1291 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2593 3152 3307 4762 618 Size: 325 217 627 806 559 155 1455 1842 Fragments arranged by size: 1842 1455 806 627 559 325 217 155 BanII G_rGCy'C Cuts at: 1391 2502 2525 2565 2854 2976 3275 3305 1391 Size: 1111 23 40 289 122 299 30 4072 Fragments arranged by size: 4072 1111 299 289 122 40 30 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2421 2641 928 Size: 971 522 220 4273 Fragments arranged by size: 4273 971 522 220 BbvCI CC'TCA_GC Cuts at: 2508 2508 Size: 5986 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4177 4211 1903 Size: 34 2240 34 3678 Fragments arranged by size: 3678 2240 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2676 2725 4597 Size: 107 122 71 631 605 1004 49 1872 Cuts at: 4597 136 Size: 1525 Fragments arranged by size: 1872 1525 1004 631 605 122 107 71 49 BglII A'GATC_T Cuts at: 2986 5981 2986 Size: 2995 2991 BlpI GC'TnA_GC Cuts at: 2798 3403 2798 Size: 605 5381 Bme1580I G_kGCm'C Cuts at: 1790 2565 2854 2868 2976 3111 3275 4047 5203 Size: 775 289 14 108 135 164 772 1156 Cuts at: 5203 1790 Size: 2573 Fragments arranged by size: 2573 1156 775 772 289 164 135 108 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2444 2890 3717 Size: 416 53 83 399 24 1027 446 827 Cuts at: 3717 4675 442 Size: 958 1753 Fragments arranged by size: 1753 1027 958 827 446 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 3185 4646 1239 Size: 848 1098 1461 2579 Fragments arranged by size: 2579 1461 1098 848 Bpu10I CC'TnA_GC Cuts at: 2496 2508 2496 Size: 12 5974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4111 4976 5274 4111 Size: 865 298 4823 Fragments arranged by size: 4823 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4649 882 Size: 617 3150 2219 Fragments arranged by size: 3150 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2785 3114 3777 493 Size: 711 1581 329 663 2702 Fragments arranged by size: 2702 1581 711 663 329 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3793 4175 4810 275 Size: 53 83 186 936 2260 382 635 1451 Fragments arranged by size: 2260 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2289 2374 4419 4817 5091 5238 5649 2289 Size: 85 2045 398 274 147 411 2626 Fragments arranged by size: 2626 2045 411 398 274 147 85 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3191 3221 1178 Size: 30 165 30 1788 30 3943 Fragments arranged by size: 3943 1788 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2909 3148 1186 Size: 1723 239 4024 Fragments arranged by size: 4024 1723 239 BseYI C'CCAG_C Cuts at: 2358 2525 2853 2860 5189 5685 2358 Size: 167 328 7 2329 496 2659 Fragments arranged by size: 2659 2329 496 328 167 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3167 1163 Size: 573 1431 3982 Fragments arranged by size: 3982 1431 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3436 4197 5225 1540 Size: 275 186 1435 761 1028 2301 Fragments arranged by size: 2301 1435 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4047 4132 5203 4047 Size: 85 1071 4830 Fragments arranged by size: 4830 1071 85 BsmI GAATG_Cn' Cuts at: 3497 3590 3497 Size: 93 5893 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2502 2525 2565 2854 2868 2976 3111 Size: 399 712 23 40 289 14 108 135 Cuts at: 3111 3275 3305 4047 4132 5203 1391 Size: 164 30 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 712 399 289 164 135 108 85 40 30 23 14 BspEI T'CCGG_A Cuts at: 2374 5649 2374 Size: 3275 2711 BspHI T'CATG_A Cuts at: 3875 5605 3875 Size: 1730 4256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2229 3189 3246 3341 844 Size: 1385 960 57 95 3489 Fragments arranged by size: 3489 1385 960 95 57 BsrBI CCG'CTC Cuts at: 1394 1823 2247 3873 1394 Size: 429 424 1626 3507 Fragments arranged by size: 3507 1626 429 424 BsrDI GCAATG_nn' Cuts at: 65 1248 4481 4655 65 Size: 1183 3233 174 1396 Fragments arranged by size: 3233 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4630 4817 1700 Size: 110 2820 187 2869 Fragments arranged by size: 2869 2820 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 3089 4046 5058 3089 Size: 957 1012 4017 Fragments arranged by size: 4017 1012 957 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5986 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstXI CCAn_nnnn'nTGG Cuts at: 3275 3275 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2025 2464 2986 3316 3683 4068 4085 5537 Size: 53 439 522 330 367 385 17 1452 Cuts at: 5537 5623 5635 5981 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 522 439 385 367 346 330 86 53 17 12 Bsu36I CC'TnA_GG Cuts at: 3119 3119 Size: 5986 BtgI C'CryG_G Cuts at: 513 1065 2004 2021 3074 513 Size: 552 939 17 1053 3425 Fragments arranged by size: 3425 1053 939 552 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 2735 2846 2866 2892 3502 4296 4316 2735 Size: 111 20 26 610 794 20 4405 Fragments arranged by size: 4405 794 610 111 26 20 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2726 3433 4322 Size: 54 1425 50 275 18 896 707 889 Cuts at: 4322 5951 8 Size: 1629 43 Fragments arranged by size: 1629 1425 896 889 707 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2228 2598 3916 1353 Size: 875 370 1318 3423 Fragments arranged by size: 3423 1318 875 370 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4573 4947 5093 387 Size: 1064 138 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2600 3185 4049 4646 5433 5701 Size: 393 455 513 585 864 597 787 268 Cuts at: 5701 1239 Size: 1524 Fragments arranged by size: 1524 864 787 597 585 513 455 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2132 2233 2132 Size: 101 5885 EcoO109I rG'GnC_Cy Cuts at: 2299 2471 2561 2562 2677 2935 2972 2973 3123 Size: 172 90 1 115 258 37 1 150 Cuts at: 3123 3271 3272 2299 Size: 148 1 5013 Fragments arranged by size: 5013 258 172 150 148 115 90 37 1 1 1 EcoRI G'AATT_C Cuts at: 3295 3673 3295 Size: 378 5608 FspI TGC'GCA Cuts at: 4492 4492 Size: 5986 HaeII r_GCGC'y Cuts at: 1678 5133 1678 Size: 3455 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2715 2747 2808 2840 4627 Size: 32 135 32 1143 32 61 32 1787 Cuts at: 4627 4659 4701 4733 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1787 1143 135 61 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3330 3576 669 Size: 988 281 1392 246 3079 Fragments arranged by size: 3079 1392 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3216 3243 5100 5284 1305 Size: 1911 27 1857 184 2007 Fragments arranged by size: 2007 1911 1857 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 2728 10 Size: 54 1425 343 896 3268 Fragments arranged by size: 3268 1425 896 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3944 4303 4462 518 Size: 648 2778 359 159 2042 Fragments arranged by size: 2778 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2857 2913 3402 4079 5227 5472 1606 Size: 444 807 56 489 677 1148 245 2120 Fragments arranged by size: 2120 1148 807 677 489 444 245 56 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4495 Fragments arranged by size: 4495 939 552 NdeI CA'TA_TG Cuts at: 387 2194 387 Size: 1807 4179 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 2755 2795 3344 4889 1132 Size: 97 1526 40 549 1545 2229 Fragments arranged by size: 2229 1545 1526 549 97 40 PasI CC'CwG_GG Cuts at: 1348 3275 1348 Size: 1927 4059 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 2747 2860 1108 Size: 1639 113 4234 Fragments arranged by size: 4234 1639 113 PfoI T'CCnGG_A Cuts at: 2467 2467 Size: 5986 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PmlI CAC'GTG Cuts at: 3114 3114 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2597 2629 4055 4087 5568 5600 2597 Size: 32 1426 32 1481 32 2983 Fragments arranged by size: 2983 1481 1426 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2299 2471 2935 2299 Size: 172 464 5350 Fragments arranged by size: 5350 464 172 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PspOMI G'GGCC_C Cuts at: 2561 2850 2972 3271 2561 Size: 289 122 299 5276 Fragments arranged by size: 5276 299 289 122 PstI C_TGCA'G Cuts at: 830 3243 3338 830 Size: 2413 95 3478 Fragments arranged by size: 3478 2413 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 PvuII CAG'CTG Cuts at: 2050 2050 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SanDI GG'GwC_CC Cuts at: 2471 2471 Size: 5986 SbfI CC_TGCA'GG Cuts at: 3243 3338 3243 Size: 95 5891 ScaI AGT'ACT Cuts at: 2398 4234 5946 2398 Size: 1836 1712 2438 Fragments arranged by size: 2438 1836 1712 SfcI C'TryA_G Cuts at: 826 1045 2487 3239 3334 4469 5150 5341 826 Size: 219 1442 752 95 1135 681 191 1471 Fragments arranged by size: 1471 1442 1135 752 681 219 191 95 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 2203 3143 3313 1872 Size: 331 940 170 4545 Fragments arranged by size: 4545 940 331 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 4090 4991 5253 820 Size: 197 926 2147 901 262 1553 Fragments arranged by size: 2147 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 2171 3233 1517 Size: 654 1062 4270 Fragments arranged by size: 4270 1062 654 StyI C'CwwG_G Cuts at: 513 1065 2004 2114 2940 3126 3425 513 Size: 552 939 110 826 186 299 3074 Fragments arranged by size: 3074 939 826 552 299 186 110 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2071 2798 3216 4013 4030 4183 4368 Size: 1255 491 727 418 797 17 153 185 Cuts at: 4368 4813 325 Size: 445 1498 Fragments arranged by size: 1498 1255 797 727 491 445 418 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2396 3225 4232 Size: 274 80 33 51 1443 419 829 1007 Cuts at: 4232 5944 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1007 829 419 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3059 3091 3191 3223 5946 5978 3059 Size: 32 100 32 2723 32 3067 Fragments arranged by size: 3067 2723 100 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5986 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 2201 3141 3311 1870 Size: 331 940 170 4545 Fragments arranged by size: 4545 940 331 170 XmnI GAAnn'nnTTC Cuts at: 2418 4115 2418 Size: 1697 4289 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI