Restriction map for
pFN21AB5164
(Linear) (Six-base) MAPSORT of: KIBB5164.seq Check: 7542 from: 1 to: 1281
KIBB5164.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1281
Size: 595 686
AlwNI CAG_nnn'CTG
Cuts at: 0 510 742 855 1281
Size: 510 232 113 426
Fragments arranged by size:
510 426 232 113
ApaI G_GGCC'C
Cuts at: 0 560 849 971 1270 1281
Size: 560 289 122 299 11
Fragments arranged by size:
560 299 289 122 11
ApoI r'AATT_y
Cuts at: 0 234 1281
Size: 234 1047
AvaI C'yCGr_G
Cuts at: 0 196 1136 1281
Size: 196 940 145
Fragments arranged by size:
940 196 145
BamHI G'GATC_C
Cuts at: 0 20 1281
Size: 20 1261
BanI G'GyrC_C
Cuts at: 0 588 1147 1281
Size: 588 559 134
Fragments arranged by size:
588 559 134
BanII G_rGCy'C
Cuts at: 0 497 520 560 849 971 1270 1281
Size: 497 23 40 289 122 299 11
Fragments arranged by size:
497 299 289 122 40 23 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 416 636 1281
Size: 416 220 645
Fragments arranged by size:
645 416 220
BbvCI CC'TCA_GC
Cuts at: 0 503 1281
Size: 503 778
BglI GCCn_nnn'nGGC
Cuts at: 0 671 720 1281
Size: 671 49 561
Fragments arranged by size:
671 561 49
BglII A'GATC_T
Cuts at: 0 981 1281
Size: 981 300
BlpI GC'TnA_GC
Cuts at: 0 793 1281
Size: 793 488
Bme1580I G_kGCm'C
Cuts at: 0 560 849 863 971 1106 1270 1281
Size: 560 289 14 108 135 164 11
Fragments arranged by size:
560 289 164 135 108 14 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 439 885 1281
Size: 439 446 396
Fragments arranged by size:
446 439 396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1180 1281
Size: 82 1098 101
Fragments arranged by size:
1098 101 82
Bpu10I CC'TnA_GC
Cuts at: 0 491 503 1281
Size: 491 12 778
Fragments arranged by size:
778 491 12
BsaAI yAC'GTr
Cuts at: 0 780 1109 1281
Size: 780 329 172
Fragments arranged by size:
780 329 172
BsaWI w'CCGG_w
Cuts at: 0 284 369 1281
Size: 284 85 912
Fragments arranged by size:
912 284 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1186 1216 1281
Size: 1186 30 65
Fragments arranged by size:
1186 65 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 904 1143 1281
Size: 904 239 138
Fragments arranged by size:
904 239 138
BseYI C'CCAG_C
Cuts at: 0 353 520 848 855 1281
Size: 353 167 328 7 426
Fragments arranged by size:
426 353 328 167 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1162 1281
Size: 1162 119
Bsp1286I G_dGCh'C
Cuts at: 0 497 520 560 849 863 971 1106 1270
Size: 497 23 40 289 14 108 135 164
Cuts at: 1270 1281
Size: 11
Fragments arranged by size:
497 289 164 135 108 40 23 14
11
BspEI T'CCGG_A
Cuts at: 0 369 1281
Size: 369 912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 1184 1241 1281
Size: 224 960 57 40
Fragments arranged by size:
960 224 57 40
BsrBI CCG'CTC
Cuts at: 0 242 1281
Size: 242 1039
BssSI C'ACGA_G
Cuts at: 0 1084 1281
Size: 1084 197
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1270 1281
Size: 1270 11
BstYI r'GATC_y
Cuts at: 0 20 459 981 1281
Size: 20 439 522 300
Fragments arranged by size:
522 439 300 20
Bsu36I CC'TnA_GG
Cuts at: 0 1114 1281
Size: 1114 167
BtgI C'CryG_G
Cuts at: 0 16 1069 1281
Size: 16 1053 212
Fragments arranged by size:
1053 212 16
BtsI GCAGTG_nn'
Cuts at: 0 730 841 861 887 1281
Size: 730 111 20 26 394
Fragments arranged by size:
730 394 111 26 20
EaeI y'GGCC_r
Cuts at: 0 721 1281
Size: 721 560
EarI CTCTTCn'nnn_
Cuts at: 0 223 593 1281
Size: 223 370 688
Fragments arranged by size:
688 370 223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 595 1180 1281
Size: 82 513 585 101
Fragments arranged by size:
585 513 101 82
EcoNI CCTnn'n_nnAGG
Cuts at: 0 127 228 1281
Size: 127 101 1053
Fragments arranged by size:
1053 127 101
EcoO109I rG'GnC_Cy
Cuts at: 0 294 466 556 557 672 930 967 968
Size: 294 172 90 1 115 258 37 1
Cuts at: 968 1118 1266 1267 1281
Size: 150 148 1 14
Fragments arranged by size:
294 258 172 150 148 115 90 37
14 1 1 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 710 742 803 835 1281
Size: 710 32 61 32 446
Fragments arranged by size:
710 446 61 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1211 1238 1281
Size: 1211 27 43
Fragments arranged by size:
1211 43 27
MscI TGG'CCA
Cuts at: 0 723 1281
Size: 723 558
MspA1I CmG'CkG
Cuts at: 0 45 852 908 1281
Size: 45 807 56 373
Fragments arranged by size:
807 373 56 45
NdeI CA'TA_TG
Cuts at: 0 189 1281
Size: 189 1092
NspI r_CATG'y
Cuts at: 0 750 790 1281
Size: 750 40 491
Fragments arranged by size:
750 491 40
PasI CC'CwG_GG
Cuts at: 0 1270 1281
Size: 1270 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 742 855 1281
Size: 742 113 426
Fragments arranged by size:
742 426 113
PfoI T'CCnGG_A
Cuts at: 0 462 1281
Size: 462 819
PmlI CAC'GTG
Cuts at: 0 1109 1281
Size: 1109 172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 1281
Size: 592 32 657
Fragments arranged by size:
657 592 32
PpuMI rG'GwC_Cy
Cuts at: 0 294 466 930 1281
Size: 294 172 464 351
Fragments arranged by size:
464 351 294 172
PspOMI G'GGCC_C
Cuts at: 0 556 845 967 1266 1281
Size: 556 289 122 299 15
Fragments arranged by size:
556 299 289 122 15
PstI C_TGCA'G
Cuts at: 0 1238 1281
Size: 1238 43
PvuII CAG'CTG
Cuts at: 0 45 1281
Size: 45 1236
SanDI GG'GwC_CC
Cuts at: 0 466 1281
Size: 466 815
SbfI CC_TGCA'GG
Cuts at: 0 1238 1281
Size: 1238 43
ScaI AGT'ACT
Cuts at: 0 393 1281
Size: 393 888
SfcI C'TryA_G
Cuts at: 0 482 1234 1281
Size: 482 752 47
Fragments arranged by size:
752 482 47
SmaI CCC'GGG
Cuts at: 0 198 1138 1281
Size: 198 940 143
Fragments arranged by size:
940 198 143
StuI AGG'CCT
Cuts at: 0 166 1228 1281
Size: 166 1062 53
Fragments arranged by size:
1062 166 53
StyI C'CwwG_G
Cuts at: 0 109 935 1121 1281
Size: 109 826 186 160
Fragments arranged by size:
826 186 160 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 66 793 1211 1281
Size: 66 727 418 70
Fragments arranged by size:
727 418 70 66
TatI w'GTAC_w
Cuts at: 0 391 1220 1281
Size: 391 829 61
Fragments arranged by size:
829 391 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1054 1086 1186 1218 1281
Size: 1054 32 100 32 63
Fragments arranged by size:
1054 100 63 32 32
XmaI C'CCGG_G
Cuts at: 0 196 1136 1281
Size: 196 940 145
Fragments arranged by size:
940 196 145
XmnI GAAnn'nnTTC
Cuts at: 0 413 1281
Size: 413 868
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BsaAI BsaWI BsaXI BseRI BseYI BsgI Bsp1286I
BspEI BspMI BsrBI BssSI BstXI BstYI Bsu36I BtgI
BtsI EaeI EarI Eco57MI EcoNI EcoO109I Hin4I MmeI
MscI MspA1I NdeI NspI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuII SanDI SbfI ScaI
SfcI SmaI StuI StyI TaqII TatI TstI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI
BmgBI BmtI BplI BpuEI BsaI BsaBI BsaHI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspHI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MslI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XcmI XhoI ZraI