(Circular) (Six-base) MAPSORT of: pFN21AB5138.seq Check: 1182 from: 1 to: 7090 pFN21AB5138 7090 bp With 182 enzymes: SgfI * October 20, 2009 10:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4900 278 Size: 53 83 186 936 3364 2468 Fragments arranged by size: 3364 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4433 1937 Size: 2496 4594 Acc65I G'GTAC_C Cuts at: 4411 4411 Size: 7090 AclI AA'CG_TT Cuts at: 1563 5217 5590 1563 Size: 3654 373 3063 Fragments arranged by size: 3654 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2603 4238 4295 5153 6537 6805 1632 Size: 971 1635 57 858 1384 268 1917 Fragments arranged by size: 1917 1635 1384 971 858 268 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6893 AflIII A'CryG_T Cuts at: 1203 3187 5989 6966 1203 Size: 1984 2802 977 1327 Fragments arranged by size: 2802 1984 1327 977 AgeI A'CCGG_T Cuts at: 5921 5921 Size: 7090 AhdI GACnn_n'nnGTC Cuts at: 5819 5819 Size: 7090 AleI CACnn'nnGTG Cuts at: 1166 2567 3113 1166 Size: 1401 546 5143 Fragments arranged by size: 5143 1401 546 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3740 1184 Size: 2556 4534 AlwNI CAG_nnn'CTG Cuts at: 2965 2983 4132 4833 6405 2965 Size: 18 1149 701 1572 3650 Fragments arranged by size: 3650 1572 1149 701 18 ApaLI G'TGCA_C Cuts at: 5147 6303 5147 Size: 1156 5934 ApoI r'AATT_y Cuts at: 1493 2294 2505 2708 3295 4050 4399 4625 4777 Size: 801 211 203 587 755 349 226 152 Cuts at: 4777 6886 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 801 755 587 349 226 211 203 152 AseI AT'TA_AT Cuts at: 160 1032 3486 4371 5644 160 Size: 872 2454 885 1273 1606 Fragments arranged by size: 2454 1606 1273 885 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7090 AvaI C'yCGr_G Cuts at: 1870 1943 2182 4415 1870 Size: 73 239 2233 4545 Fragments arranged by size: 4545 2233 239 73 BamHI G'GATC_C Cuts at: 4420 4787 4420 Size: 367 6723 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2792 3922 4411 5866 618 Size: 325 217 627 1005 1130 489 1455 1842 Fragments arranged by size: 1842 1455 1130 1005 627 489 325 217 BanII G_rGCy'C Cuts at: 1391 2120 2925 3788 4409 1391 Size: 729 805 863 621 4072 Fragments arranged by size: 4072 863 805 729 621 BbeI G_GCGC'C Cuts at: 2796 2796 Size: 7090 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2541 2744 3111 3668 4037 928 Size: 971 642 203 367 557 369 3981 Fragments arranged by size: 3981 971 642 557 369 367 203 BbvCI CC'TCA_GC Cuts at: 3681 3876 3681 Size: 195 6895 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5281 5315 1903 Size: 34 3344 34 3678 Fragments arranged by size: 3678 3344 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4982 6192 4982 Size: 1210 5880 BclI T'GATC_A Cuts at: 1548 1548 Size: 7090 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5701 136 Size: 107 122 71 631 605 4029 1525 Fragments arranged by size: 4029 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3068 7085 3068 Size: 4017 3073 BlpI GC'TnA_GC Cuts at: 2976 4507 2976 Size: 1531 5559 Bme1580I G_kGCm'C Cuts at: 1790 2633 5151 6307 1790 Size: 843 2518 1156 2573 Fragments arranged by size: 2573 2518 1156 843 BmgBI CAC'GTC Cuts at: 1318 4096 1318 Size: 2778 4312 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3256 4821 5779 Size: 416 53 83 399 24 1839 1565 958 Cuts at: 5779 442 Size: 1753 Fragments arranged by size: 1839 1753 1565 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7090 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2595 3667 3807 2595 Size: 1072 140 5878 Fragments arranged by size: 5878 1072 140 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 3606 3984 4061 5750 1239 Size: 884 1483 378 77 1689 2579 Fragments arranged by size: 2579 1689 1483 884 378 77 Bpu10I CC'TnA_GC Cuts at: 3681 3876 3681 Size: 195 6895 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3525 4184 5215 6080 6378 3525 Size: 659 1031 865 298 4237 Fragments arranged by size: 4237 1031 865 659 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3318 5753 882 Size: 617 1819 2435 2219 Fragments arranged by size: 2435 2219 1819 617 BsaAI yAC'GTr Cuts at: 493 1204 2437 3188 4881 493 Size: 711 1233 751 1693 2702 Fragments arranged by size: 2702 1693 1233 751 711 BsaBI GATnn'nnATC Cuts at: 2484 2604 7084 2484 Size: 120 4480 2490 Fragments arranged by size: 4480 2490 120 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2793 4897 5279 5914 Size: 53 83 186 936 1260 2104 382 635 Cuts at: 5914 275 Size: 1451 Fragments arranged by size: 2104 1451 1260 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2892 3310 4229 5523 5921 6195 6342 6753 2892 Size: 418 919 1294 398 274 147 411 3229 Fragments arranged by size: 3229 1294 919 418 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2134 2164 1178 Size: 30 165 30 731 30 6104 Fragments arranged by size: 6104 731 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 3864 4280 1186 Size: 1111 1567 416 3996 Fragments arranged by size: 3996 1567 1111 416 BseYI C'CCAG_C Cuts at: 2784 3018 3407 4140 6293 6789 2784 Size: 234 389 733 2153 496 3085 Fragments arranged by size: 3085 2153 733 496 389 234 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2897 3837 1163 Size: 573 1161 940 4416 Fragments arranged by size: 4416 1161 940 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4540 5301 6329 1540 Size: 275 186 2539 761 1028 2301 Fragments arranged by size: 2539 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5151 5236 6307 5151 Size: 85 1071 5934 Fragments arranged by size: 5934 1071 85 BsmI GAATG_Cn' Cuts at: 2581 3367 4601 4694 2581 Size: 786 1234 93 4977 Fragments arranged by size: 4977 1234 786 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 2633 2925 3788 4409 5151 5236 Size: 399 330 513 292 863 621 742 85 Cuts at: 5236 6307 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 863 742 621 513 399 330 292 85 BspEI T'CCGG_A Cuts at: 2892 6753 2892 Size: 3861 3229 BspHI T'CATG_A Cuts at: 4979 6709 4979 Size: 1730 5360 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2919 4445 844 Size: 2075 1526 3489 Fragments arranged by size: 3489 2075 1526 BsrBI CCG'CTC Cuts at: 1394 1823 4977 1394 Size: 429 3154 3507 Fragments arranged by size: 3507 3154 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2345 5585 5759 65 Size: 1183 1097 3240 174 1396 Fragments arranged by size: 3240 1396 1183 1097 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5734 5921 1700 Size: 110 3924 187 2869 Fragments arranged by size: 3924 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7090 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7090 BssSI C'ACGA_G Cuts at: 5150 6162 5150 Size: 1012 6078 BstAPI GCAn_nnn'nTGC Cuts at: 794 2167 3551 3560 794 Size: 1373 1384 9 4324 Fragments arranged by size: 4324 1384 1373 9 BstBI TT'CG_AA Cuts at: 5894 5894 Size: 7090 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7090 BstYI r'GATC_y Cuts at: 1972 3068 3613 4420 4787 5172 5189 6641 6727 Size: 1096 545 807 367 385 17 1452 86 Cuts at: 6727 6739 7085 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1096 807 545 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2207 2207 Size: 7090 BtgI C'CryG_G Cuts at: 513 1065 2004 2872 3375 3733 3857 3977 3987 Size: 552 939 868 503 358 124 120 10 Cuts at: 3987 4207 513 Size: 220 3396 Fragments arranged by size: 3396 939 868 552 503 358 220 124 120 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3159 487 Size: 683 56 1933 4418 Fragments arranged by size: 4418 1933 683 56 BtsI GCAGTG_nn' Cuts at: 3006 4606 5400 5420 3006 Size: 1600 794 20 4676 Fragments arranged by size: 4676 1600 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7090 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3784 3819 529 Size: 35 3220 35 3800 Fragments arranged by size: 3800 3220 35 35 DraI TTT'AAA Cuts at: 2175 4394 2175 Size: 2219 4871 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6097 809 Size: 507 4781 1802 Fragments arranged by size: 4781 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2619 2812 3372 Size: 54 1425 50 275 18 789 193 560 Cuts at: 3372 4037 4537 5426 7055 8 Size: 665 500 889 1629 43 Fragments arranged by size: 1629 1425 889 789 665 560 500 275 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4537 1537 Size: 275 2725 4090 Fragments arranged by size: 4090 2725 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2122 2907 3234 4110 4257 4281 4335 5020 Size: 769 785 327 876 147 24 54 685 Cuts at: 5020 1353 Size: 3423 Fragments arranged by size: 3423 876 785 769 685 327 147 54 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5677 6051 6197 387 Size: 1064 138 4088 374 146 1280 Fragments arranged by size: 4088 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2603 3606 3984 4061 4238 4295 Size: 393 491 480 1003 378 77 177 57 Cuts at: 4295 5153 5750 6537 6805 1239 Size: 858 597 787 268 1524 Fragments arranged by size: 1524 1003 858 787 597 491 480 393 378 268 177 77 57 EcoNI CCTnn'n_nnAGG Cuts at: 3233 3233 Size: 7090 EcoO109I rG'GnC_Cy Cuts at: 3244 3380 3962 3244 Size: 136 582 6372 Fragments arranged by size: 6372 582 136 EcoRI G'AATT_C Cuts at: 4399 4777 4399 Size: 378 6712 FspI TGC'GCA Cuts at: 2862 5596 2862 Size: 2734 4356 HaeII r_GCGC'y Cuts at: 1678 2796 6237 1678 Size: 1118 3441 2531 Fragments arranged by size: 3441 2531 1118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3150 3182 3949 3981 5731 Size: 32 135 32 1578 32 767 32 1750 Cuts at: 5731 5763 5805 5837 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1750 1578 767 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2956 4434 4680 669 Size: 988 281 1018 1478 246 3079 Fragments arranged by size: 3079 1478 1018 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7090 HpaI GTT'AAC Cuts at: 4680 4680 Size: 7090 KasI G'GCGC_C Cuts at: 2792 2792 Size: 7090 KpnI G_GTAC'C Cuts at: 4415 4415 Size: 7090 MfeI C'AATT_G Cuts at: 2948 4689 2948 Size: 1741 5349 MluI A'CGCG_T Cuts at: 6966 6966 Size: 7090 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2386 6204 6388 1305 Size: 1081 3818 184 2007 Fragments arranged by size: 3818 2007 1081 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2621 2814 10 Size: 54 1425 343 789 193 4286 Fragments arranged by size: 4286 1425 789 343 193 54 MslI CAynn'nnrTG Cuts at: 518 1166 2567 3113 3732 5048 5407 5566 518 Size: 648 1401 546 619 1316 359 159 2042 Fragments arranged by size: 2042 1401 1316 648 619 546 359 159 MspA1I CmG'CkG Cuts at: 1606 2332 2965 2980 4189 4506 5183 6331 6576 Size: 726 633 15 1209 317 677 1148 245 Cuts at: 6576 1606 Size: 2120 Fragments arranged by size: 2120 1209 1148 726 677 633 317 245 15 NarI GG'CG_CC Cuts at: 2793 2793 Size: 7090 NcoI C'CATG_G Cuts at: 513 1065 2004 2872 3375 3733 3857 513 Size: 552 939 868 503 358 124 3746 Fragments arranged by size: 3746 939 868 552 503 358 124 NdeI CA'TA_TG Cuts at: 387 387 Size: 7090 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7090 NotI GC'GGCC_GC Cuts at: 4537 4537 Size: 7090 NspI r_CATG'y Cuts at: 1132 1229 2291 4448 5993 1132 Size: 97 1062 2157 1545 2229 Fragments arranged by size: 2229 2157 1545 1062 97 PasI CC'CwG_GG Cuts at: 1348 2918 1348 Size: 1570 5520 PciI A'CATG_T Cuts at: 5989 5989 Size: 7090 PflMI CCAn_nnn'nTGG Cuts at: 1108 2965 1108 Size: 1857 5233 PmeI GTTT'AAAC Cuts at: 4394 4394 Size: 7090 PmlI CAC'GTG Cuts at: 3188 3188 Size: 7090 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5159 5191 6672 6704 5159 Size: 32 1481 32 5545 Fragments arranged by size: 5545 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3244 3380 3244 Size: 136 6954 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7090 PsiI TTA'TAA Cuts at: 4660 4660 Size: 7090 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2657 2689 4175 4207 2657 Size: 32 1486 32 5540 Fragments arranged by size: 5540 1486 32 32 PstI C_TGCA'G Cuts at: 830 4442 830 Size: 3612 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7090 PvuII CAG'CTG Cuts at: 2332 2965 2980 2332 Size: 633 15 6442 Fragments arranged by size: 6442 633 15 SalI G'TCGA_C Cuts at: 1936 4432 1936 Size: 2496 4594 SapI GCTCTTCn'nnn_ Cuts at: 4335 4335 Size: 7090 SbfI CC_TGCA'GG Cuts at: 4442 4442 Size: 7090 ScaI AGT'ACT Cuts at: 2671 5338 7050 2671 Size: 2667 1712 2711 Fragments arranged by size: 2711 2667 1712 SfcI C'TryA_G Cuts at: 826 1045 2457 4438 5573 6254 6445 826 Size: 219 1412 1981 1135 681 191 1471 Fragments arranged by size: 1981 1471 1412 1135 681 219 191 SfoI GGC'GCC Cuts at: 2794 2794 Size: 7090 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7090 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7090 SmaI CCC'GGG Cuts at: 1872 4417 1872 Size: 2545 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3540 4199 5194 6095 6357 820 Size: 197 926 1597 659 995 901 262 1553 Fragments arranged by size: 1597 1553 995 926 901 659 262 197 SnaBI TAC'GTA Cuts at: 493 4881 493 Size: 4388 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7090 SphI G_CATG'C Cuts at: 1132 2291 4448 1132 Size: 1159 2157 3774 Fragments arranged by size: 3774 2157 1159 SspI AAT'ATT Cuts at: 5 52 5014 5 Size: 47 4962 2081 Fragments arranged by size: 4962 2081 47 StuI AGG'CCT Cuts at: 1517 3840 1517 Size: 2323 4767 StyI C'CwwG_G Cuts at: 513 1065 2004 2622 2872 3375 3733 3802 3857 Size: 552 939 618 250 503 358 69 55 Cuts at: 3857 4065 4529 513 Size: 208 464 3074 Fragments arranged by size: 3074 939 618 552 503 464 358 250 208 69 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3342 3421 5117 5134 5287 5472 5917 Size: 1255 1762 79 1696 17 153 185 445 Cuts at: 5917 325 Size: 1498 Fragments arranged by size: 1762 1696 1498 1255 445 185 153 79 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2144 2669 4158 Size: 274 80 33 51 1443 167 525 1489 Cuts at: 4158 5336 7048 96 Size: 1178 1712 138 Fragments arranged by size: 1712 1489 1443 1178 525 274 167 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2134 2166 7050 7082 2134 Size: 32 4884 32 2142 Fragments arranged by size: 4884 2142 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4132 1316 Size: 2816 4274 XbaI T'CTAG_A Cuts at: 3818 4426 3818 Size: 608 6482 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7090 XmaI C'CCGG_G Cuts at: 1870 4415 1870 Size: 2545 4545 XmnI GAAnn'nnTTC Cuts at: 5219 5219 Size: 7090 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4898 276 Size: 53 83 186 936 3364 2468 Fragments arranged by size: 3364 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI