(Circular) (Six-base) MAPSORT of: pFN21AB5109.seq Check: 5640 from: 1 to: 6523 pFN21AB5109 6523 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2035 2886 2035 Size: 851 5672 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2793 4333 278 Size: 53 83 186 936 1257 1540 2468 Fragments arranged by size: 2468 1540 1257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3866 1937 Size: 1929 4594 Acc65I G'GTAC_C Cuts at: 3844 3844 Size: 6523 AclI AA'CG_TT Cuts at: 1563 4650 5023 1563 Size: 3087 373 3063 Fragments arranged by size: 3087 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2358 3304 3320 3461 4586 5970 6238 1632 Size: 726 946 16 141 1125 1384 268 1917 Fragments arranged by size: 1917 1384 1125 946 726 268 141 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6326 AflIII A'CryG_T Cuts at: 1203 5422 6399 1203 Size: 4219 977 1327 Fragments arranged by size: 4219 1327 977 AgeI A'CCGG_T Cuts at: 5354 5354 Size: 6523 AhdI GACnn_n'nnGTC Cuts at: 3290 5252 3290 Size: 1962 4561 AleI CACnn'nnGTG Cuts at: 1166 2706 1166 Size: 1540 4983 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2765 2957 1184 Size: 1581 192 4750 Fragments arranged by size: 4750 1581 192 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3092 3124 3092 Size: 32 6491 AlwNI CAG_nnn'CTG Cuts at: 2851 2980 4266 5838 2851 Size: 129 1286 1572 3536 Fragments arranged by size: 3536 1572 1286 129 ApaI G_GGCC'C Cuts at: 3476 3476 Size: 6523 ApaLI G'TGCA_C Cuts at: 3362 4580 5736 3362 Size: 1218 1156 4149 Fragments arranged by size: 4149 1218 1156 ApoI r'AATT_y Cuts at: 1493 3832 4058 4210 6319 1493 Size: 2339 226 152 2109 1697 Fragments arranged by size: 2339 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3042 5077 160 Size: 872 2010 2035 1606 Fragments arranged by size: 2035 2010 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 AvaI C'yCGr_G Cuts at: 1870 1943 3254 3848 1870 Size: 73 1311 594 4545 Fragments arranged by size: 4545 1311 594 73 BamHI G'GATC_C Cuts at: 3853 4220 3853 Size: 367 6156 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3431 3506 3844 5299 618 Size: 325 217 627 1644 75 338 1455 1842 Fragments arranged by size: 1842 1644 1455 627 338 325 217 75 BanII G_rGCy'C Cuts at: 1391 2831 3348 3476 3842 1391 Size: 1440 517 128 366 4072 Fragments arranged by size: 4072 1440 517 366 128 BbeI G_GCGC'C Cuts at: 3510 3510 Size: 6523 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2346 2386 2666 928 Size: 971 447 40 280 4785 Fragments arranged by size: 4785 971 447 280 40 BbvCI CC'TCA_GC Cuts at: 2249 2249 Size: 6523 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4714 4748 1903 Size: 34 50 34 2693 34 3678 Fragments arranged by size: 3678 2693 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2254 3254 4415 5625 2254 Size: 1000 1161 1210 3152 Fragments arranged by size: 3152 1210 1161 1000 BclI T'GATC_A Cuts at: 1548 1548 Size: 6523 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2485 5134 136 Size: 107 122 71 631 605 813 2649 1525 Fragments arranged by size: 2649 1525 813 631 605 122 107 71 BglII A'GATC_T Cuts at: 6518 6518 Size: 6523 BlpI GC'TnA_GC Cuts at: 3940 3940 Size: 6523 Bme1580I G_kGCm'C Cuts at: 1790 3366 3434 3476 4584 5740 1790 Size: 1576 68 42 1108 1156 2573 Fragments arranged by size: 2573 1576 1156 1108 68 42 BmgBI CAC'GTC Cuts at: 1318 2947 3487 1318 Size: 1629 540 4354 Fragments arranged by size: 4354 1629 540 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2673 3058 4254 Size: 416 53 83 399 24 1256 385 1196 Cuts at: 4254 5212 442 Size: 958 1753 Fragments arranged by size: 1753 1256 1196 958 416 399 385 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6523 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3091 3091 Size: 6523 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5183 1239 Size: 3944 2579 Bpu10I CC'TnA_GC Cuts at: 2249 2249 Size: 6523 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4648 5513 5811 4648 Size: 865 298 5360 Fragments arranged by size: 5360 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5186 882 Size: 617 3687 2219 Fragments arranged by size: 3687 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4314 493 Size: 711 3110 2702 Fragments arranged by size: 3110 2702 711 BsaBI GATnn'nnATC Cuts at: 2645 6517 2645 Size: 3872 2651 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2058 2790 3507 3528 Size: 53 83 186 936 525 732 717 21 Cuts at: 3528 4330 4712 5347 275 Size: 802 382 635 1451 Fragments arranged by size: 1451 936 802 732 717 635 525 382 186 83 53 21 BsaWI w'CCGG_w Cuts at: 2228 3650 4956 5354 5628 5775 6186 2228 Size: 1422 1306 398 274 147 411 2565 Fragments arranged by size: 2565 1422 1306 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2210 2240 2267 2297 2660 Size: 30 165 30 807 30 27 30 363 Cuts at: 2660 2690 3632 3662 1178 Size: 30 942 30 4039 Fragments arranged by size: 4039 942 807 363 165 30 30 30 30 30 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6523 BseYI C'CCAG_C Cuts at: 2146 2207 2309 2683 2851 2930 3083 5726 6222 Size: 61 102 374 168 79 153 2643 496 Cuts at: 6222 2146 Size: 2447 Fragments arranged by size: 2643 2447 496 374 168 153 102 79 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2927 3716 1163 Size: 573 1191 789 3970 Fragments arranged by size: 3970 1191 789 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3973 4734 5762 1540 Size: 275 186 1972 761 1028 2301 Fragments arranged by size: 2301 1972 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 2280 3366 3768 4584 4669 5740 2175 Size: 105 1086 402 816 85 1071 2958 Fragments arranged by size: 2958 1086 1071 816 402 105 85 BsiWI C'GTAC_G Cuts at: 2010 2010 Size: 6523 BsmI GAATG_Cn' Cuts at: 2519 4034 4127 2519 Size: 1515 93 4915 Fragments arranged by size: 4915 1515 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2065 3053 2065 Size: 988 5535 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2280 2831 3348 3366 3434 3476 Size: 399 385 105 551 517 18 68 42 Cuts at: 3476 3768 3842 4584 4669 5740 1391 Size: 292 74 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 551 517 399 385 292 105 85 74 68 42 18 BspEI T'CCGG_A Cuts at: 2228 6186 2228 Size: 3958 2565 BspHI T'CATG_A Cuts at: 4412 6142 4412 Size: 1730 4793 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2035 2886 3878 844 Size: 1191 851 992 3489 Fragments arranged by size: 3489 1191 992 851 BsrBI CCG'CTC Cuts at: 1394 1823 4410 1394 Size: 429 2587 3507 Fragments arranged by size: 3507 2587 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2220 2325 5018 5192 65 Size: 1183 972 105 2693 174 1396 Fragments arranged by size: 2693 1396 1183 972 174 105 BsrFI r'CCGG_y Cuts at: 1700 1810 2475 2484 3726 5167 5354 1700 Size: 110 665 9 1242 1441 187 2869 Fragments arranged by size: 2869 1441 1242 665 187 110 9 BsrGI T'GTAC_A Cuts at: 96 2103 96 Size: 2007 4516 BssHII G'CGCG_C Cuts at: 1925 2679 1925 Size: 754 5769 BssSI C'ACGA_G Cuts at: 4583 5595 4583 Size: 1012 5511 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6523 BstBI TT'CG_AA Cuts at: 5327 5327 Size: 6523 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6523 BstXI CCAn_nnnn'nTGG Cuts at: 2708 2839 3538 2708 Size: 131 699 5693 Fragments arranged by size: 5693 699 131 BstYI r'GATC_y Cuts at: 1972 2253 3711 3853 4220 4605 4622 6074 6160 Size: 281 1458 142 367 385 17 1452 86 Cuts at: 6160 6172 6518 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1458 1452 385 367 346 281 142 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2085 2129 2085 Size: 44 6479 BtgI C'CryG_G Cuts at: 513 1065 2358 2493 2805 2823 2832 3120 3267 Size: 552 1293 135 312 18 9 288 147 Cuts at: 3267 513 Size: 3769 Fragments arranged by size: 3769 1293 552 312 288 147 135 18 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2517 487 Size: 683 56 1291 4493 Fragments arranged by size: 4493 1291 683 56 BtsI GCAGTG_nn' Cuts at: 3366 4039 4833 4853 3366 Size: 673 794 20 5036 Fragments arranged by size: 5036 794 673 20 ClaI AT'CG_AT Cuts at: 1554 2969 1554 Size: 1415 5108 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6488 DraI TTT'AAA Cuts at: 3827 3827 Size: 6523 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3407 5530 809 Size: 507 2091 2123 1802 Fragments arranged by size: 2123 2091 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2477 2490 Size: 54 1425 50 275 18 363 284 13 Cuts at: 2490 2635 3970 4859 6488 8 Size: 145 1335 889 1629 43 Fragments arranged by size: 1629 1425 1335 889 363 284 275 145 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 3970 1537 Size: 275 2158 4090 Fragments arranged by size: 4090 2158 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2419 4453 1353 Size: 1066 2034 3423 Fragments arranged by size: 3423 2034 1066 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2703 3401 5110 5484 5630 387 Size: 1064 138 1114 698 1709 374 146 1280 Fragments arranged by size: 1709 1280 1114 1064 698 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2358 3304 3320 3461 4586 5183 5970 Size: 393 726 946 16 141 1125 597 787 Cuts at: 5970 6238 1239 Size: 268 1524 Fragments arranged by size: 1524 1125 946 787 726 597 393 268 141 16 EcoNI CCTnn'n_nnAGG Cuts at: 2042 3299 2042 Size: 1257 5266 EcoO109I rG'GnC_Cy Cuts at: 2531 3472 3473 2531 Size: 941 1 5581 Fragments arranged by size: 5581 941 1 EcoRI G'AATT_C Cuts at: 3832 4210 3832 Size: 378 6145 FspI TGC'GCA Cuts at: 5029 5029 Size: 6523 HaeII r_GCGC'y Cuts at: 1678 3510 5670 1678 Size: 1832 2160 2531 Fragments arranged by size: 2531 2160 1832 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2396 2428 2962 2994 3276 Size: 32 135 32 824 32 534 32 282 Cuts at: 3276 3308 3554 3586 3696 3728 5164 5196 5238 Size: 32 246 32 110 32 1436 32 42 Cuts at: 5238 5270 1373 Size: 32 2626 Fragments arranged by size: 2626 1436 824 534 282 246 135 110 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2581 3867 4113 669 Size: 988 281 643 1286 246 3079 Fragments arranged by size: 3079 1286 988 643 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6523 HpaI GTT'AAC Cuts at: 4113 4113 Size: 6523 KasI G'GCGC_C Cuts at: 3506 3506 Size: 6523 KpnI G_GTAC'C Cuts at: 3848 3848 Size: 6523 MfeI C'AATT_G Cuts at: 3387 4122 3387 Size: 735 5788 MluI A'CGCG_T Cuts at: 6399 6399 Size: 6523 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2708 5637 5821 1305 Size: 1403 2929 184 2007 Fragments arranged by size: 2929 2007 1403 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2195 2492 10 Size: 54 1425 343 363 297 4041 Fragments arranged by size: 4041 1425 363 343 297 54 MslI CAynn'nnrTG Cuts at: 518 1166 2706 3662 3668 4481 4840 4999 518 Size: 648 1540 956 6 813 359 159 2042 Fragments arranged by size: 2042 1540 956 813 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2211 2851 3147 3201 3939 4616 5764 6009 Size: 605 640 296 54 738 677 1148 245 Cuts at: 6009 1606 Size: 2120 Fragments arranged by size: 2120 1148 738 677 640 605 296 245 54 NaeI GCC'GGC Cuts at: 2477 2477 Size: 6523 NarI GG'CG_CC Cuts at: 3507 3507 Size: 6523 NcoI C'CATG_G Cuts at: 513 1065 2823 513 Size: 552 1758 4213 Fragments arranged by size: 4213 1758 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6523 NgoMIV G'CCGG_C Cuts at: 2475 2475 Size: 6523 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6523 NotI GC'GGCC_GC Cuts at: 3970 3970 Size: 6523 NspI r_CATG'y Cuts at: 1132 1229 3881 5426 1132 Size: 97 2652 1545 2229 Fragments arranged by size: 2652 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6523 PciI A'CATG_T Cuts at: 5422 5422 Size: 6523 PflMI CCAn_nnn'nTGG Cuts at: 1108 2185 2784 2851 1108 Size: 1077 599 67 4780 Fragments arranged by size: 4780 1077 599 67 PfoI T'CCnGG_A Cuts at: 3110 3110 Size: 6523 PmeI GTTT'AAAC Cuts at: 3827 3827 Size: 6523 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4592 4624 6105 6137 4592 Size: 32 1481 32 4978 Fragments arranged by size: 4978 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2531 2531 Size: 6523 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6523 PsiI TTA'TAA Cuts at: 4093 4093 Size: 6523 PspOMI G'GGCC_C Cuts at: 3472 3472 Size: 6523 PstI C_TGCA'G Cuts at: 830 3152 3875 830 Size: 2322 723 3478 Fragments arranged by size: 3478 2322 723 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6523 PvuII CAG'CTG Cuts at: 2851 3147 2851 Size: 296 6227 SalI G'TCGA_C Cuts at: 1936 3865 1936 Size: 1929 4594 SanDI GG'GwC_CC Cuts at: 2531 2531 Size: 6523 SapI GCTCTTCn'nnn_ Cuts at: 2419 2419 Size: 6523 SbfI CC_TGCA'GG Cuts at: 3875 3875 Size: 6523 ScaI AGT'ACT Cuts at: 4771 6483 4771 Size: 1712 4811 SexAI A'CCwGG_T Cuts at: 3102 3102 Size: 6523 SfcI C'TryA_G Cuts at: 826 1045 3148 3196 3871 5006 5687 5878 826 Size: 219 2103 48 675 1135 681 191 1471 Fragments arranged by size: 2103 1471 1135 681 675 219 191 48 SfoI GGC'GCC Cuts at: 3508 3508 Size: 6523 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6523 SmaI CCC'GGG Cuts at: 1872 3256 3850 1872 Size: 1384 594 4545 Fragments arranged by size: 4545 1384 594 SmlI C'TyrA_G Cuts at: 820 1017 1943 4627 5528 5790 820 Size: 197 926 2684 901 262 1553 Fragments arranged by size: 2684 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4314 493 Size: 3821 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6523 SphI G_CATG'C Cuts at: 1132 3881 1132 Size: 2749 3774 SspI AAT'ATT Cuts at: 5 52 4447 5 Size: 47 4395 2081 Fragments arranged by size: 4395 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6523 StyI C'CwwG_G Cuts at: 513 1065 2823 3468 3962 513 Size: 552 1758 645 494 3074 Fragments arranged by size: 3074 1758 645 552 494 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2759 3497 4550 4567 4720 4905 5350 Size: 1255 1179 738 1053 17 153 185 445 Cuts at: 5350 325 Size: 1498 Fragments arranged by size: 1498 1255 1179 1053 738 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2103 4769 6481 Size: 274 80 33 51 1443 126 2666 1712 Cuts at: 6481 96 Size: 138 Fragments arranged by size: 2666 1712 1443 274 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2660 2692 3252 3284 3632 3664 3673 3705 6483 Size: 32 560 32 348 32 9 32 2778 Cuts at: 6483 6515 2660 Size: 32 2668 Fragments arranged by size: 2778 2668 560 348 32 32 32 32 32 9 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6523 XbaI T'CTAG_A Cuts at: 3859 3859 Size: 6523 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2306 3118 2306 Size: 812 5711 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6523 XmaI C'CCGG_G Cuts at: 1870 3254 3848 1870 Size: 1384 594 4545 Fragments arranged by size: 4545 1384 594 XmnI GAAnn'nnTTC Cuts at: 2027 3317 4652 2027 Size: 1290 1335 3898 Fragments arranged by size: 3898 1335 1290 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2791 4331 276 Size: 53 83 186 936 1257 1540 2468 Fragments arranged by size: 2468 1540 1257 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SfiI SrfI SwaI