(Circular) (Six-base) MAPSORT of: pFN21AB5108.seq Check: 6945 from: 1 to: 7090 pFN21AB5108 7090 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4900 278 Size: 53 83 186 936 3364 2468 Fragments arranged by size: 3364 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4433 1937 Size: 2496 4594 Acc65I G'GTAC_C Cuts at: 2025 4411 2025 Size: 2386 4704 AclI AA'CG_TT Cuts at: 1563 5217 5590 1563 Size: 3654 373 3063 Fragments arranged by size: 3654 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2946 3689 3714 3750 3776 3843 5153 6537 Size: 1314 743 25 36 26 67 1310 1384 Cuts at: 6537 6805 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1314 1310 743 268 67 36 26 25 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6893 AflIII A'CryG_T Cuts at: 1203 5989 6966 1203 Size: 4786 977 1327 Fragments arranged by size: 4786 1327 977 AgeI A'CCGG_T Cuts at: 2264 5921 2264 Size: 3657 3433 AhdI GACnn_n'nnGTC Cuts at: 5819 5819 Size: 7090 AleI CACnn'nnGTG Cuts at: 1166 3876 1166 Size: 2710 4380 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7090 AlwNI CAG_nnn'CTG Cuts at: 3494 3515 4833 6405 3494 Size: 21 1318 1572 4179 Fragments arranged by size: 4179 1572 1318 21 ApaI G_GGCC'C Cuts at: 2146 2146 Size: 7090 ApaLI G'TGCA_C Cuts at: 5147 6303 5147 Size: 1156 5934 ApoI r'AATT_y Cuts at: 1493 2369 2615 3203 3350 4381 4399 4625 4777 Size: 876 246 588 147 1031 18 226 152 Cuts at: 4777 6886 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1031 876 588 246 226 152 147 18 AseI AT'TA_AT Cuts at: 160 1032 5644 160 Size: 872 4612 1606 Fragments arranged by size: 4612 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7090 AvaI C'yCGr_G Cuts at: 1870 1943 2118 4415 1870 Size: 73 175 2297 4545 Fragments arranged by size: 4545 2297 175 73 BamHI G'GATC_C Cuts at: 4420 4787 4420 Size: 367 6723 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2025 4411 5866 618 Size: 325 217 627 238 2386 1455 1842 Fragments arranged by size: 2386 1842 1455 627 325 238 217 BanII G_rGCy'C Cuts at: 1391 2146 3446 3916 4409 1391 Size: 755 1300 470 493 4072 Fragments arranged by size: 4072 1300 755 493 470 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2583 928 Size: 971 684 5435 Fragments arranged by size: 5435 971 684 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5281 5315 1903 Size: 34 3344 34 3678 Fragments arranged by size: 3678 3344 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4982 6192 4982 Size: 1210 5880 BclI T'GATC_A Cuts at: 1548 1548 Size: 7090 BfrBI ATG'CAT Cuts at: 2220 2289 2220 Size: 69 7021 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5701 136 Size: 107 122 71 631 605 4029 1525 Fragments arranged by size: 4029 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2478 2762 7085 2478 Size: 284 4323 2483 Fragments arranged by size: 4323 2483 284 BlpI GC'TnA_GC Cuts at: 2033 3006 4507 2033 Size: 973 1501 4616 Fragments arranged by size: 4616 1501 973 Bme1580I G_kGCm'C Cuts at: 1790 2146 5151 6307 1790 Size: 356 3005 1156 2573 Fragments arranged by size: 3005 2573 1156 356 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7090 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3870 4821 5779 Size: 416 53 83 399 24 2453 951 958 Cuts at: 5779 442 Size: 1753 Fragments arranged by size: 2453 1753 958 951 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7090 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5750 1239 Size: 4511 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2215 3752 5215 6080 6378 2215 Size: 1537 1463 865 298 2927 Fragments arranged by size: 2927 1537 1463 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2115 5753 882 Size: 617 616 3638 2219 Fragments arranged by size: 3638 2219 617 616 BsaAI yAC'GTr Cuts at: 493 1204 4881 493 Size: 711 3677 2702 Fragments arranged by size: 3677 2702 711 BsaBI GATnn'nnATC Cuts at: 3321 7084 3321 Size: 3763 3327 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4897 5279 5914 275 Size: 53 83 186 936 3364 382 635 1451 Fragments arranged by size: 3364 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2264 5523 5921 6195 6342 6753 2264 Size: 3259 398 274 147 411 2601 Fragments arranged by size: 3259 2601 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2091 2121 3097 3127 1178 Size: 30 165 30 688 30 976 30 5141 Fragments arranged by size: 5141 976 688 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7090 BseYI C'CCAG_C Cuts at: 3398 6293 6789 3398 Size: 2895 496 3699 Fragments arranged by size: 3699 2895 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3672 3825 1163 Size: 573 1936 153 4428 Fragments arranged by size: 4428 1936 573 153 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4540 5301 6329 1540 Size: 275 186 2539 761 1028 2301 Fragments arranged by size: 2539 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2797 2844 5151 5236 6307 2797 Size: 47 2307 85 1071 3580 Fragments arranged by size: 3580 2307 1071 85 47 BsiWI C'GTAC_G Cuts at: 2065 2065 Size: 7090 BsmI GAATG_Cn' Cuts at: 4367 4601 4694 4367 Size: 234 93 6763 Fragments arranged by size: 6763 234 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2146 2797 2844 3446 3916 4409 5151 Size: 399 356 651 47 602 470 493 742 Cuts at: 5151 5236 6307 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 651 602 493 470 399 356 85 47 BspEI T'CCGG_A Cuts at: 6753 6753 Size: 7090 BspHI T'CATG_A Cuts at: 4029 4979 6709 4029 Size: 950 1730 4410 Fragments arranged by size: 4410 1730 950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4445 844 Size: 3601 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2013 4977 1394 Size: 429 190 2964 3507 Fragments arranged by size: 3507 2964 429 190 BsrDI GCAATG_nn' Cuts at: 65 1248 3904 4101 5585 5759 65 Size: 1183 2656 197 1484 174 1396 Fragments arranged by size: 2656 1484 1396 1183 197 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2028 2264 5734 5921 1700 Size: 110 218 236 3470 187 2869 Fragments arranged by size: 3470 2869 236 218 187 110 BsrGI T'GTAC_A Cuts at: 96 4282 96 Size: 4186 2904 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7090 BssSI C'ACGA_G Cuts at: 2054 5150 6162 2054 Size: 3096 1012 2982 Fragments arranged by size: 3096 2982 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2636 794 Size: 1842 5248 BstBI TT'CG_AA Cuts at: 5894 5894 Size: 7090 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7090 BstYI r'GATC_y Cuts at: 1972 2478 2762 3409 3452 3478 4420 4787 5172 Size: 506 284 647 43 26 942 367 385 Cuts at: 5172 5189 6641 6727 6739 7085 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 942 647 506 385 367 346 284 86 43 26 17 12 BtgI C'CryG_G Cuts at: 513 1065 3534 513 Size: 552 2469 4069 Fragments arranged by size: 4069 2469 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6351 Fragments arranged by size: 6351 683 56 BtsI GCAGTG_nn' Cuts at: 2403 2469 4606 5400 5420 2403 Size: 66 2137 794 20 4073 Fragments arranged by size: 4073 2137 794 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7090 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3220 3255 529 Size: 35 2656 35 4364 Fragments arranged by size: 4364 2656 35 35 DraI TTT'AAA Cuts at: 4394 4394 Size: 7090 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3063 6097 809 Size: 507 1747 3034 1802 Fragments arranged by size: 3034 1802 1747 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2191 3537 4009 Size: 54 1425 50 275 18 361 1346 472 Cuts at: 4009 4537 5426 7055 8 Size: 528 889 1629 43 Fragments arranged by size: 1629 1425 1346 889 528 472 361 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4537 1537 Size: 275 2725 4090 Fragments arranged by size: 4090 2725 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3451 3690 5020 1353 Size: 2098 239 1330 3423 Fragments arranged by size: 3423 2098 1330 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2082 5677 6051 6197 387 Size: 1064 138 493 3595 374 146 1280 Fragments arranged by size: 3595 1280 1064 493 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2946 3689 3714 3750 3776 3843 5153 Size: 393 1314 743 25 36 26 67 1310 Cuts at: 5153 5750 6537 6805 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1314 1310 787 743 597 393 268 67 36 26 25 EcoNI CCTnn'n_nnAGG Cuts at: 3486 3486 Size: 7090 EcoO109I rG'GnC_Cy Cuts at: 2071 2143 2071 Size: 72 7018 EcoRI G'AATT_C Cuts at: 4399 4777 4399 Size: 378 6712 FspI TGC'GCA Cuts at: 5596 5596 Size: 7090 HaeII r_GCGC'y Cuts at: 1678 6237 1678 Size: 4559 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2747 2779 3013 3045 3730 Size: 32 135 32 1175 32 234 32 685 Cuts at: 3730 3762 4011 4043 5731 5763 5805 5837 1373 Size: 32 249 32 1688 32 42 32 2626 Fragments arranged by size: 2626 1688 1175 685 249 234 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2505 3288 3708 4434 4680 669 Size: 988 281 567 783 420 726 246 3079 Fragments arranged by size: 3079 988 783 726 567 420 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7090 HpaI GTT'AAC Cuts at: 3288 3708 4680 3288 Size: 420 972 5698 Fragments arranged by size: 5698 972 420 KpnI G_GTAC'C Cuts at: 2029 4415 2029 Size: 2386 4704 MfeI C'AATT_G Cuts at: 3222 3997 4689 3222 Size: 775 692 5623 Fragments arranged by size: 5623 775 692 MluI A'CGCG_T Cuts at: 6966 6966 Size: 7090 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2830 6204 6388 1305 Size: 1525 3374 184 2007 Fragments arranged by size: 3374 2007 1525 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2193 4011 10 Size: 54 1425 343 361 1818 3089 Fragments arranged by size: 3089 1818 1425 361 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3033 3876 5048 5407 5566 518 Size: 648 1867 843 1172 359 159 2042 Fragments arranged by size: 2042 1867 1172 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2459 3581 3973 4506 5183 6331 6576 1606 Size: 853 1122 392 533 677 1148 245 2120 Fragments arranged by size: 2120 1148 1122 853 677 533 392 245 NcoI C'CATG_G Cuts at: 513 1065 3534 513 Size: 552 2469 4069 Fragments arranged by size: 4069 2469 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7090 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7090 NotI GC'GGCC_GC Cuts at: 4537 4537 Size: 7090 NsiI A_TGCA'T Cuts at: 2222 2291 2222 Size: 69 7021 NspI r_CATG'y Cuts at: 1132 1229 4448 5993 1132 Size: 97 3219 1545 2229 Fragments arranged by size: 3219 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7090 PciI A'CATG_T Cuts at: 5989 5989 Size: 7090 PflMI CCAn_nnn'nTGG Cuts at: 1108 2059 1108 Size: 951 6139 PmeI GTTT'AAAC Cuts at: 4394 4394 Size: 7090 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5159 5191 6672 6704 5159 Size: 32 1481 32 5545 Fragments arranged by size: 5545 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7090 PsiI TTA'TAA Cuts at: 4660 4660 Size: 7090 PspOMI G'GGCC_C Cuts at: 2142 2142 Size: 7090 PstI C_TGCA'G Cuts at: 830 2464 4442 830 Size: 1634 1978 3478 Fragments arranged by size: 3478 1978 1634 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7090 PvuII CAG'CTG Cuts at: 2459 3581 3973 2459 Size: 1122 392 5576 Fragments arranged by size: 5576 1122 392 SalI G'TCGA_C Cuts at: 1936 4432 1936 Size: 2496 4594 SapI GCTCTTCn'nnn_ Cuts at: 3451 3451 Size: 7090 SbfI CC_TGCA'GG Cuts at: 4442 4442 Size: 7090 ScaI AGT'ACT Cuts at: 5338 7050 5338 Size: 1712 5378 SexAI A'CCwGG_T Cuts at: 2021 3040 3508 2021 Size: 1019 468 5603 Fragments arranged by size: 5603 1019 468 SfcI C'TryA_G Cuts at: 826 1045 2460 4366 4438 5573 6254 6445 826 Size: 219 1415 1906 72 1135 681 191 1471 Fragments arranged by size: 1906 1471 1415 1135 681 219 191 72 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7090 SgrAI Cr'CCGG_yG Cuts at: 1700 2264 1700 Size: 564 6526 SmaI CCC'GGG Cuts at: 1872 4417 1872 Size: 2545 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2230 3731 5194 6095 6357 820 Size: 197 926 287 1501 1463 901 262 1553 Fragments arranged by size: 1553 1501 1463 926 901 287 262 197 SnaBI TAC'GTA Cuts at: 493 4881 493 Size: 4388 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7090 SphI G_CATG'C Cuts at: 1132 4448 1132 Size: 3316 3774 SspI AAT'ATT Cuts at: 5 52 3601 3657 5014 5 Size: 47 3549 56 1357 2081 Fragments arranged by size: 3549 2081 1357 56 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7090 StyI C'CwwG_G Cuts at: 513 1065 3281 3534 4529 513 Size: 552 2216 253 995 3074 Fragments arranged by size: 3074 2216 995 552 253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5117 5134 5287 5472 5917 325 Size: 1255 3537 17 153 185 445 1498 Fragments arranged by size: 3537 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4210 4282 5336 Size: 274 80 33 51 1443 2233 72 1054 Cuts at: 5336 7048 96 Size: 1712 138 Fragments arranged by size: 2233 1712 1443 1054 274 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2091 2123 3097 3129 3463 3495 7050 7082 2091 Size: 32 974 32 334 32 3555 32 2099 Fragments arranged by size: 3555 2099 974 334 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7090 XbaI T'CTAG_A Cuts at: 4426 4426 Size: 7090 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7090 XmaI C'CCGG_G Cuts at: 1870 4415 1870 Size: 2545 4545 XmnI GAAnn'nnTTC Cuts at: 5219 5219 Size: 7090 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4898 276 Size: 53 83 186 936 3364 2468 Fragments arranged by size: 3364 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI