(Circular) (Six-base) MAPSORT of: pFN21AB5098.seq Check: 4635 from: 1 to: 8179 pFN21AB5098 8179 bp With 182 enzymes: SgfI * March 28, 2012 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4702 4702 Size: 8179 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5989 278 Size: 53 83 186 936 4453 2468 Fragments arranged by size: 4453 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3639 5522 1937 Size: 1702 1883 4594 Fragments arranged by size: 4594 1883 1702 Acc65I G'GTAC_C Cuts at: 5500 5500 Size: 8179 AclI AA'CG_TT Cuts at: 1563 6306 6679 1563 Size: 4743 373 3063 Fragments arranged by size: 4743 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3041 3421 4570 6242 7626 7894 1632 Size: 1409 380 1149 1672 1384 268 1917 Fragments arranged by size: 1917 1672 1409 1384 1149 380 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7982 AflIII A'CryG_T Cuts at: 1203 3958 7078 8055 1203 Size: 2755 3120 977 1327 Fragments arranged by size: 3120 2755 1327 977 AgeI A'CCGG_T Cuts at: 3266 7010 3266 Size: 3744 4435 AhdI GACnn_n'nnGTC Cuts at: 6908 6908 Size: 8179 AleI CACnn'nnGTG Cuts at: 1166 3521 5267 1166 Size: 2355 1746 4078 Fragments arranged by size: 4078 2355 1746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3285 3905 3989 1184 Size: 2101 620 84 5374 Fragments arranged by size: 5374 2101 620 84 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3839 3871 3839 Size: 32 8147 AlwNI CAG_nnn'CTG Cuts at: 3928 4357 5922 7494 3928 Size: 429 1565 1572 4613 Fragments arranged by size: 4613 1572 1565 429 ApaLI G'TGCA_C Cuts at: 4715 6236 7392 4715 Size: 1521 1156 5502 Fragments arranged by size: 5502 1521 1156 ApoI r'AATT_y Cuts at: 1493 5488 5714 5866 7975 1493 Size: 3995 226 152 2109 1697 Fragments arranged by size: 3995 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4794 6733 160 Size: 872 3762 1939 1606 Fragments arranged by size: 3762 1939 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8179 AvaI C'yCGr_G Cuts at: 1870 1943 2117 2151 2183 2576 2801 3851 4380 Size: 73 174 34 32 393 225 1050 529 Cuts at: 4380 4846 4888 5300 5324 5372 5504 1870 Size: 466 42 412 24 48 132 4545 Fragments arranged by size: 4545 1050 529 466 412 393 225 174 132 73 48 42 34 32 24 AvrII C'CTAG_G Cuts at: 5134 5134 Size: 8179 BamHI G'GATC_C Cuts at: 3873 5509 5876 3873 Size: 1636 367 6176 Fragments arranged by size: 6176 1636 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2023 2073 2317 2602 2757 Size: 325 217 627 236 50 244 285 155 Cuts at: 2757 3269 3693 3997 5112 5500 6955 618 Size: 512 424 304 1115 388 1455 1842 Fragments arranged by size: 1842 1455 1115 627 512 424 388 325 304 285 244 236 217 155 50 BanII G_rGCy'C Cuts at: 1391 2151 2190 2823 3105 3537 3785 3852 4046 Size: 760 39 633 282 432 248 67 194 Cuts at: 4046 4491 4617 5205 5301 5325 5498 1391 Size: 445 126 588 96 24 173 4072 Fragments arranged by size: 4072 760 633 588 445 432 282 248 194 173 126 96 67 39 24 BbeI G_GCGC'C Cuts at: 2027 2077 2606 2761 5116 2027 Size: 50 529 155 2355 5090 Fragments arranged by size: 5090 2355 529 155 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4814 928 Size: 971 2915 4293 Fragments arranged by size: 4293 2915 971 BbvCI CC'TCA_GC Cuts at: 4140 4140 Size: 8179 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3299 3333 4519 4553 6370 6404 1903 Size: 34 1362 34 1186 34 1817 34 3678 Fragments arranged by size: 3678 1817 1362 1186 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4234 4321 6071 7281 4234 Size: 87 1750 1210 5132 Fragments arranged by size: 5132 1750 1210 87 BclI T'GATC_A Cuts at: 1548 1548 Size: 8179 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 6790 136 Size: 107 122 71 631 605 337 4781 1525 Fragments arranged by size: 4781 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3798 3813 8174 3798 Size: 15 4361 3803 Fragments arranged by size: 4361 3803 15 BlpI GC'TnA_GC Cuts at: 2033 2988 5596 2033 Size: 955 2608 4616 Fragments arranged by size: 4616 2608 955 Bme1580I G_kGCm'C Cuts at: 1790 2066 2322 3241 3749 4002 4719 5098 6240 Size: 276 256 919 508 253 717 379 1142 Cuts at: 6240 7396 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1142 919 717 508 379 276 256 253 BmgBI CAC'GTC Cuts at: 1318 2164 1318 Size: 846 7333 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3322 3851 4069 Size: 416 53 83 399 24 1905 529 218 Cuts at: 4069 5332 5910 6868 442 Size: 1263 578 958 1753 Fragments arranged by size: 1905 1753 1263 958 578 529 416 399 218 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8179 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2088 2561 3379 3629 4407 4631 6839 1239 Size: 849 473 818 250 778 224 2208 2579 Fragments arranged by size: 2579 2208 849 818 778 473 250 224 Bpu10I CC'TnA_GC Cuts at: 2145 2670 4140 5186 2145 Size: 525 1470 1046 5138 Fragments arranged by size: 5138 1470 1046 525 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3779 6304 7169 7467 3779 Size: 2525 865 298 4491 Fragments arranged by size: 4491 2525 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2633 2678 3053 4408 5249 5327 5351 Size: 617 1134 45 375 1355 841 78 24 Cuts at: 5351 5444 6842 882 Size: 93 1398 2219 Fragments arranged by size: 2219 1398 1355 1134 841 617 375 93 78 45 24 BsaAI yAC'GTr Cuts at: 493 1204 2923 3959 5970 493 Size: 711 1719 1036 2011 2702 Fragments arranged by size: 2702 2011 1719 1036 711 BsaBI GATnn'nnATC Cuts at: 8173 8173 Size: 8179 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2024 2074 2603 2758 Size: 53 83 186 936 491 50 529 155 Cuts at: 2758 3137 3372 5053 5068 5113 5986 6368 7003 Size: 379 235 1681 15 45 873 382 635 Cuts at: 7003 275 Size: 1451 Fragments arranged by size: 1681 1451 936 873 635 529 491 382 379 235 186 155 83 53 50 45 15 BsaWI w'CCGG_w Cuts at: 2263 3266 4440 6612 7010 7284 7431 7842 2263 Size: 1003 1174 2172 398 274 147 411 2600 Fragments arranged by size: 2600 2172 1174 1003 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3116 3146 3839 3869 4388 Size: 30 165 30 1713 30 693 30 519 Cuts at: 4388 4418 5137 5167 5288 5312 5318 5336 5342 Size: 30 719 30 121 24 6 18 6 Cuts at: 5342 5366 1178 Size: 24 3991 Fragments arranged by size: 3991 1713 719 693 519 165 121 30 30 30 30 30 30 24 24 18 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2594 2651 2693 2696 2809 2843 2870 Size: 937 471 57 42 3 113 34 27 Cuts at: 2870 2955 3029 3071 3131 3140 3143 3202 3755 Size: 85 74 42 60 9 3 59 553 Cuts at: 3755 4097 4157 5402 5405 5408 5411 5420 5423 Size: 342 60 1245 3 3 3 9 3 Cuts at: 5423 1186 Size: 3942 Fragments arranged by size: 3942 1245 937 553 471 342 113 85 74 60 60 59 57 42 42 34 27 9 9 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2564 2845 3616 3869 3892 4066 4276 5235 5361 Size: 281 771 253 23 174 210 959 126 Cuts at: 5361 7382 7878 2564 Size: 2021 496 2865 Fragments arranged by size: 2865 2021 959 771 496 281 253 210 174 126 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3995 1163 Size: 573 2259 5347 Fragments arranged by size: 5347 2259 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 2250 3250 5629 6390 7418 Size: 275 186 12 237 1000 2379 761 1028 Cuts at: 7418 1540 Size: 2301 Fragments arranged by size: 2379 2301 1028 1000 761 275 237 186 12 BsiHKAI G_wGCw'C Cuts at: 2664 3537 3626 3785 4131 4617 4719 5192 6240 Size: 873 89 159 346 486 102 473 1048 Cuts at: 6240 6325 7396 2664 Size: 85 1071 3447 Fragments arranged by size: 3447 1071 1048 873 486 473 346 159 102 89 85 BsmI GAATG_Cn' Cuts at: 5429 5690 5783 5429 Size: 261 93 7825 Fragments arranged by size: 7825 261 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2151 2190 2322 2664 2823 3105 Size: 399 276 85 39 132 342 159 282 Cuts at: 3105 3241 3537 3626 3749 3785 3852 4002 4046 Size: 136 296 89 123 36 67 150 44 Cuts at: 4046 4131 4491 4617 4719 5098 5192 5205 5301 Size: 85 360 126 102 379 94 13 96 Cuts at: 5301 5325 5498 6240 6325 7396 1391 Size: 24 173 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 379 360 342 296 282 276 173 159 150 136 132 126 123 102 96 94 89 85 85 85 67 44 39 36 24 13 BspEI T'CCGG_A Cuts at: 2263 7842 2263 Size: 5579 2600 BspHI T'CATG_A Cuts at: 3723 4648 6068 7798 3723 Size: 925 1420 1730 4104 Fragments arranged by size: 4104 1730 1420 925 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4602 4702 4910 5534 844 Size: 3758 100 208 624 3489 Fragments arranged by size: 3758 3489 624 208 100 BsrBI CCG'CTC Cuts at: 1394 1823 2806 4303 6066 1394 Size: 429 983 1497 1763 3507 Fragments arranged by size: 3507 1763 1497 983 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3448 4245 4761 4912 6674 6848 65 Size: 1183 2200 797 516 151 1762 174 1396 Fragments arranged by size: 2200 1762 1396 1183 797 516 174 151 BsrFI r'CCGG_y Cuts at: 1700 1810 3249 3266 3544 4523 5001 6823 7010 Size: 110 1439 17 278 979 478 1822 187 Cuts at: 7010 1700 Size: 2869 Fragments arranged by size: 2869 1822 1439 979 478 278 187 110 17 BsrGI T'GTAC_A Cuts at: 96 3465 96 Size: 3369 4810 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8179 BssSI C'ACGA_G Cuts at: 3786 6239 7251 3786 Size: 2453 1012 4714 Fragments arranged by size: 4714 2453 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2086 3597 794 Size: 1292 1511 5376 Fragments arranged by size: 5376 1511 1292 BstBI TT'CG_AA Cuts at: 6983 6983 Size: 8179 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8179 BstXI CCAn_nnnn'nTGG Cuts at: 3172 3582 4609 3172 Size: 410 1027 6742 Fragments arranged by size: 6742 1027 410 BstYI r'GATC_y Cuts at: 1972 3201 3798 3813 3873 4114 5509 5876 6261 Size: 1229 597 15 60 241 1395 367 385 Cuts at: 6261 6278 7730 7816 7828 8174 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1395 1229 597 385 367 346 241 86 60 17 15 12 Bsu36I CC'TnA_GG Cuts at: 2412 2826 2412 Size: 414 7765 BtgI C'CryG_G Cuts at: 513 1065 2004 2195 3226 3807 3877 4218 4227 Size: 552 939 191 1031 581 70 341 9 Cuts at: 4227 4368 4495 4720 513 Size: 141 127 225 3972 Fragments arranged by size: 3972 1031 939 581 552 341 225 191 141 127 70 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3960 5010 487 Size: 683 56 2734 1050 3656 Fragments arranged by size: 3656 2734 1050 683 56 BtsI GCAGTG_nn' Cuts at: 2549 3081 3864 5695 6489 6509 2549 Size: 532 783 1831 794 20 4219 Fragments arranged by size: 4219 1831 794 783 532 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8179 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8144 DraI TTT'AAA Cuts at: 5483 5483 Size: 8179 DraIII CAC_nnn'GTG Cuts at: 3997 4804 4935 5149 3997 Size: 807 131 214 7027 Fragments arranged by size: 7027 807 214 131 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7186 809 Size: 507 5870 1802 Fragments arranged by size: 5870 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2237 2247 Size: 54 1425 50 275 18 180 227 10 Cuts at: 2247 2546 2813 3247 3669 4359 4986 5422 5626 Size: 299 267 434 422 690 627 436 204 Cuts at: 5626 6515 8144 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 690 627 436 434 422 299 275 267 227 204 180 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2010 2247 3247 5626 1537 Size: 275 198 237 1000 2379 4090 Fragments arranged by size: 4090 2379 1000 275 237 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2501 3000 5379 5397 6109 1353 Size: 1148 499 2379 18 712 3423 Fragments arranged by size: 3423 2379 1148 712 499 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2958 6766 7140 7286 387 Size: 1064 138 1369 3808 374 146 1280 Fragments arranged by size: 3808 1369 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3535 3783 4615 3535 Size: 248 832 7099 Fragments arranged by size: 7099 832 248 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2561 3041 3379 3421 3629 4407 Size: 393 456 473 480 338 42 208 778 Cuts at: 4407 4570 4631 6242 6839 7626 7894 1239 Size: 163 61 1611 597 787 268 1524 Fragments arranged by size: 1611 1524 787 778 597 480 473 456 393 338 268 208 163 61 42 EcoNI CCTnn'n_nnAGG Cuts at: 3974 3974 Size: 8179 EcoO109I rG'GnC_Cy Cuts at: 2170 2309 2340 2511 2836 3324 4156 4168 5138 Size: 139 31 171 325 488 832 12 970 Cuts at: 5138 5209 2170 Size: 71 5140 Fragments arranged by size: 5140 970 832 488 325 171 139 71 31 12 EcoRI G'AATT_C Cuts at: 5488 5866 5488 Size: 378 7801 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2347 4285 2347 Size: 1938 6241 FspI TGC'GCA Cuts at: 3965 6685 3965 Size: 2720 5459 HaeII r_GCGC'y Cuts at: 1678 2027 2077 2606 2761 2995 4067 4694 4896 Size: 349 50 529 155 234 1072 627 202 Cuts at: 4896 5116 7326 1678 Size: 220 2210 2531 Fragments arranged by size: 2531 2210 1072 627 529 349 234 220 202 155 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2854 2886 4106 4138 4388 Size: 32 135 32 1282 32 1220 32 250 Cuts at: 4388 4420 6820 6852 6894 6926 1373 Size: 32 2400 32 42 32 2626 Fragments arranged by size: 2626 2400 1282 1220 250 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4543 4912 5523 5769 669 Size: 988 281 2605 369 611 246 3079 Fragments arranged by size: 3079 2605 988 611 369 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8179 HpaI GTT'AAC Cuts at: 5769 5769 Size: 8179 KasI G'GCGC_C Cuts at: 2023 2073 2602 2757 5112 2023 Size: 50 529 155 2355 5090 Fragments arranged by size: 5090 2355 529 155 50 KpnI G_GTAC'C Cuts at: 5504 5504 Size: 8179 MfeI C'AATT_G Cuts at: 5778 5778 Size: 8179 MluI A'CGCG_T Cuts at: 8055 8055 Size: 8179 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3102 4983 5226 5322 7293 7477 1305 Size: 1797 1881 243 96 1971 184 2007 Fragments arranged by size: 2007 1971 1881 1797 243 184 96 MscI TGG'CCA Cuts at: 10 64 1489 1832 2548 2815 3671 4988 5424 Size: 54 1425 343 716 267 856 1317 436 Cuts at: 5424 10 Size: 2765 Fragments arranged by size: 2765 1425 1317 856 716 436 343 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 3170 3521 3728 4653 4703 4781 4988 Size: 648 2004 351 207 925 50 78 207 Cuts at: 4988 5267 6137 6496 6655 518 Size: 279 870 359 159 2042 Fragments arranged by size: 2042 2004 925 870 648 359 351 279 207 207 159 78 50 MspA1I CmG'CkG Cuts at: 1606 2037 2197 2896 4354 4497 4722 5231 5365 Size: 431 160 699 1458 143 225 509 134 Cuts at: 5365 5595 6272 7420 7665 1606 Size: 230 677 1148 245 2120 Fragments arranged by size: 2120 1458 1148 699 677 509 431 245 230 225 160 143 134 NaeI GCC'GGC Cuts at: 3251 3251 Size: 8179 NarI GG'CG_CC Cuts at: 2024 2074 2603 2758 5113 2024 Size: 50 529 155 2355 5090 Fragments arranged by size: 5090 2355 529 155 50 NcoI C'CATG_G Cuts at: 513 1065 2004 3807 3877 4368 513 Size: 552 939 1803 70 491 4324 Fragments arranged by size: 4324 1803 939 552 491 70 NdeI CA'TA_TG Cuts at: 387 387 Size: 8179 NgoMIV G'CCGG_C Cuts at: 3249 3249 Size: 8179 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8179 NotI GC'GGCC_GC Cuts at: 5626 5626 Size: 8179 NspI r_CATG'y Cuts at: 1132 1229 3262 3604 3715 5537 7082 1132 Size: 97 2033 342 111 1822 1545 2229 Fragments arranged by size: 2229 2033 1822 1545 342 111 97 PasI CC'CwG_GG Cuts at: 1348 3382 3827 5205 1348 Size: 2034 445 1378 4322 Fragments arranged by size: 4322 2034 1378 445 PciI A'CATG_T Cuts at: 7078 7078 Size: 8179 PflMI CCAn_nnn'nTGG Cuts at: 1108 3232 1108 Size: 2124 6055 PfoI T'CCnGG_A Cuts at: 2629 4110 4974 2629 Size: 1481 864 5834 Fragments arranged by size: 5834 1481 864 PmeI GTTT'AAAC Cuts at: 5483 5483 Size: 8179 PmlI CAC'GTG Cuts at: 3959 3959 Size: 8179 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 6248 6280 7761 7793 3839 Size: 32 2377 32 1481 32 4225 Fragments arranged by size: 4225 2377 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2340 3324 4156 4168 2340 Size: 984 832 12 6351 Fragments arranged by size: 6351 984 832 12 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8179 PsiI TTA'TAA Cuts at: 5749 5749 Size: 8179 PspXI vC'TCGA_Gb Cuts at: 2117 2117 Size: 8179 PstI C_TGCA'G Cuts at: 830 5061 5531 830 Size: 4231 470 3478 Fragments arranged by size: 4231 3478 470 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8179 PvuII CAG'CTG Cuts at: 2037 4354 5365 2037 Size: 2317 1011 4851 Fragments arranged by size: 4851 2317 1011 RsrII CG'GwC_CG Cuts at: 4566 4566 Size: 8179 SacI G_AGCT'C Cuts at: 3537 3785 4617 3537 Size: 248 832 7099 Fragments arranged by size: 7099 832 248 SacII CC_GC'GG Cuts at: 2198 4498 4723 2198 Size: 2300 225 5654 Fragments arranged by size: 5654 2300 225 SalI G'TCGA_C Cuts at: 1936 5521 1936 Size: 3585 4594 SanDI GG'GwC_CC Cuts at: 2340 4156 4168 2340 Size: 1816 12 6351 Fragments arranged by size: 6351 1816 12 SbfI CC_TGCA'GG Cuts at: 5531 5531 Size: 8179 ScaI AGT'ACT Cuts at: 6427 8139 6427 Size: 1712 6467 SexAI A'CCwGG_T Cuts at: 3993 3993 Size: 8179 SfcI C'TryA_G Cuts at: 826 1045 3640 5057 5527 6662 7343 7534 826 Size: 219 2595 1417 470 1135 681 191 1471 Fragments arranged by size: 2595 1471 1417 1135 681 470 219 191 SfoI GGC'GCC Cuts at: 2025 2075 2604 2759 5114 2025 Size: 50 529 155 2355 5090 Fragments arranged by size: 5090 2355 529 155 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8179 SgrAI Cr'CCGG_yG Cuts at: 1700 4523 1700 Size: 2823 5356 SmaI CCC'GGG Cuts at: 1872 2153 4382 4848 5506 1872 Size: 281 2229 466 658 4545 Fragments arranged by size: 4545 2229 658 466 281 SmlI C'TyrA_G Cuts at: 820 1017 1943 2117 3794 6283 7184 7446 820 Size: 197 926 174 1677 2489 901 262 1553 Fragments arranged by size: 2489 1677 1553 926 901 262 197 174 SnaBI TAC'GTA Cuts at: 493 5970 493 Size: 5477 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8179 SphI G_CATG'C Cuts at: 1132 3604 5537 1132 Size: 2472 1933 3774 Fragments arranged by size: 3774 2472 1933 SspI AAT'ATT Cuts at: 5 52 6103 5 Size: 47 6051 2081 Fragments arranged by size: 6051 2081 47 StuI AGG'CCT Cuts at: 1517 3792 1517 Size: 2275 5904 StyI C'CwwG_G Cuts at: 513 1065 2004 2313 2322 2331 2379 2397 2430 Size: 552 939 309 9 9 48 18 33 Cuts at: 2430 2445 2478 3051 3807 3877 4368 5134 5313 Size: 15 33 573 756 70 491 766 179 Cuts at: 5313 5618 513 Size: 305 3074 Fragments arranged by size: 3074 939 766 756 573 552 491 309 305 179 70 48 33 33 18 15 9 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2108 2392 2458 2491 2702 3077 3395 Size: 1255 528 284 66 33 211 375 318 Cuts at: 3395 3448 4499 5303 6206 6223 6376 6561 7006 Size: 53 1051 804 903 17 153 185 445 Cuts at: 7006 325 Size: 1498 Fragments arranged by size: 1498 1255 1051 903 804 528 445 375 318 284 211 185 153 66 53 33 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2748 3465 6425 Size: 274 80 33 51 1443 771 717 2960 Cuts at: 6425 8137 96 Size: 1712 138 Fragments arranged by size: 2960 1712 1443 771 717 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3116 3148 3943 3975 5137 5169 8139 8171 3116 Size: 32 795 32 1162 32 2970 32 3124 Fragments arranged by size: 3124 2970 1162 795 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4400 1316 Size: 3084 5095 XbaI T'CTAG_A Cuts at: 5515 5515 Size: 8179 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2329 3427 3679 2329 Size: 1098 252 6829 Fragments arranged by size: 6829 1098 252 XhoI C'TCGA_G Cuts at: 1943 2117 1943 Size: 174 8005 XmaI C'CCGG_G Cuts at: 1870 2151 4380 4846 5504 1870 Size: 281 2229 466 658 4545 Fragments arranged by size: 4545 2229 658 466 281 XmnI GAAnn'nnTTC Cuts at: 4268 6308 4268 Size: 2040 6139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5987 276 Size: 53 83 186 936 4453 2468 Fragments arranged by size: 4453 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspOMI PsrI SapI SfiI SrfI SwaI