Restriction map for
pFN21AB5078
(Circular) (Six-base) MAPSORT of: pFN21AB5078.seq Check: 3418 from: 1 to: 7276
pFN21AB5078 7276 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2050 3631 4411 2050
Size: 1581 780 4915
Fragments arranged by size:
4915 1581 780
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5086 278
Size: 53 83 186 936 3550 2468
Fragments arranged by size:
3550 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2337 4619 1937
Size: 400 2282 4594
Fragments arranged by size:
4594 2282 400
Acc65I G'GTAC_C
Cuts at: 4597 4597
Size: 7276
AclI AA'CG_TT
Cuts at: 1563 5403 5776 1563
Size: 3840 373 3063
Fragments arranged by size:
3840 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2813 2936 4035 5339 6723 6991 1632
Size: 1181 123 1099 1304 1384 268 1917
Fragments arranged by size:
1917 1384 1304 1181 1099 268 123
AfeI AGC'GCT
Cuts at: 3050 3746 3050
Size: 696 6580
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7079
AflIII A'CryG_T
Cuts at: 1203 2157 3598 6175 7152 1203
Size: 954 1441 2577 977 1327
Fragments arranged by size:
2577 1441 1327 977 954
AgeI A'CCGG_T
Cuts at: 6107 6107
Size: 7276
AhdI GACnn_n'nnGTC
Cuts at: 6005 6005
Size: 7276
AleI CACnn'nnGTG
Cuts at: 1166 2525 3836 1166
Size: 1359 1311 4606
Fragments arranged by size:
4606 1359 1311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7276
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 7244
AlwNI CAG_nnn'CTG
Cuts at: 2663 3676 4342 4403 4522 5019 6591 2663
Size: 1013 666 61 119 497 1572 3348
Fragments arranged by size:
3348 1572 1013 666 497 119 61
ApaLI G'TGCA_C
Cuts at: 2914 5333 6489 2914
Size: 2419 1156 3701
Fragments arranged by size:
3701 2419 1156
ApoI r'AATT_y
Cuts at: 1493 3185 4585 4811 4963 7072 1493
Size: 1692 1400 226 152 2109 1697
Fragments arranged by size:
2109 1697 1692 1400 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5830 160
Size: 872 4798 1606
Fragments arranged by size:
4798 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7276
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 4601 1870
Size: 73 77 2581 4545
Fragments arranged by size:
4545 2581 77 73
BamHI G'GATC_C
Cuts at: 4606 4973 4606
Size: 367 6909
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2071 2499 3241 4009 4271
Size: 325 217 627 284 428 742 768 262
Cuts at: 4271 4597 6052 618
Size: 326 1455 1842
Fragments arranged by size:
1842 1455 768 742 627 428 326 325
284 262 217
BanII G_rGCy'C
Cuts at: 1391 3945 4335 4595 1391
Size: 2554 390 260 4072
Fragments arranged by size:
4072 2554 390 260
BbeI G_GCGC'C
Cuts at: 3245 3245
Size: 7276
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3578 3924 928
Size: 971 1679 346 4280
Fragments arranged by size:
4280 1679 971 346
BbvCI CC'TCA_GC
Cuts at: 3588 3588
Size: 7276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5467 5501 1903
Size: 34 3530 34 3678
Fragments arranged by size:
3678 3530 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2973 4543 5168 6378 2973
Size: 1570 625 1210 3871
Fragments arranged by size:
3871 1570 1210 625
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7276
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2085 4260 5887
Size: 107 122 71 631 605 413 2175 1627
Cuts at: 5887 136
Size: 1525
Fragments arranged by size:
2175 1627 1525 631 605 413 122 107
71
BglII A'GATC_T
Cuts at: 2253 7271 2253
Size: 5018 2258
BlpI GC'TnA_GC
Cuts at: 4693 4693
Size: 7276
Bme1580I G_kGCm'C
Cuts at: 1790 2918 4274 4438 5337 6493 1790
Size: 1128 1356 164 899 1156 2573
Fragments arranged by size:
2573 1356 1156 1128 899 164
BmgBI CAC'GTC
Cuts at: 1318 2158 1318
Size: 840 6436
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5007 5965 442
Size: 416 53 83 399 24 3590 958 1753
Fragments arranged by size:
3590 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2405 2868 1056
Size: 1349 463 5464
Fragments arranged by size:
5464 1349 463
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3629
Size: 7276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2912 3099 4319 5936 1239
Size: 1673 187 1220 1617 2579
Fragments arranged by size:
2579 1673 1617 1220 187
Bpu10I CC'TnA_GC
Cuts at: 3588 3588
Size: 7276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2942 3383 3506 3677 5401 6266 6564 2942
Size: 441 123 171 1724 865 298 3654
Fragments arranged by size:
3654 1724 865 441 298 171 123
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2392 5939 882
Size: 617 893 3547 2219
Fragments arranged by size:
3547 2219 893 617
BsaAI yAC'GTr
Cuts at: 493 1204 4369 5067 493
Size: 711 3165 698 2702
Fragments arranged by size:
3165 2702 711 698
BsaBI GATnn'nnATC
Cuts at: 3338 3449 7270 3338
Size: 111 3821 3344
Fragments arranged by size:
3821 3344 111
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3242 3575 5083 5465
Size: 53 83 186 936 1709 333 1508 382
Cuts at: 5465 6100 275
Size: 635 1451
Fragments arranged by size:
1709 1508 1451 936 635 382 333 186
83 53
BsaWI w'CCGG_w
Cuts at: 5709 6107 6381 6528 6939 5709
Size: 398 274 147 411 6046
Fragments arranged by size:
6046 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3494 3524 3774 3804 1178
Size: 30 165 30 2091 30 250 30 4650
Fragments arranged by size:
4650 2091 250 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3143 3935 1186
Size: 1957 792 4527
Fragments arranged by size:
4527 1957 792
BseYI C'CCAG_C
Cuts at: 2572 3041 3802 3863 4189 4193 4396 6479 6975
Size: 469 761 61 326 4 203 2083 496
Cuts at: 6975 2572
Size: 2873
Fragments arranged by size:
2873 2083 761 496 469 326 203 61
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3659 4139 4270 4556 1163
Size: 573 1923 480 131 286 3883
Fragments arranged by size:
3883 1923 573 480 286 131
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4726 5487 6515 1540
Size: 275 186 1264 1461 761 1028 2301
Fragments arranged by size:
2301 1461 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2918 3399 3945 4335 5337 5422 6493 2918
Size: 481 546 390 1002 85 1071 3701
Fragments arranged by size:
3701 1071 1002 546 481 390 85
BsiWI C'GTAC_G
Cuts at: 4369 4369
Size: 7276
BsmI GAATG_Cn'
Cuts at: 2427 3157 4787 4880 2427
Size: 730 1630 93 4823
Fragments arranged by size:
4823 1630 730 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2918 3399 3945 4274 4335 4438 4595
Size: 399 1128 481 546 329 61 103 157
Cuts at: 4595 5337 5422 6493 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1128 1071 742 546 481 399 329
157 103 85 61
BspEI T'CCGG_A
Cuts at: 6939 6939
Size: 7276
BspHI T'CATG_A
Cuts at: 5165 6895 5165
Size: 1730 5546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2050 2052 3631 4411 4528 4631 844
Size: 1206 2 1579 780 117 103 3489
Fragments arranged by size:
3489 1579 1206 780 117 103 2
BsrBI CCG'CTC
Cuts at: 1394 1823 2535 3113 5163 1394
Size: 429 712 578 2050 3507
Fragments arranged by size:
3507 2050 712 578 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3276 4507 5771 5945 65
Size: 1183 2028 1231 1264 174 1396
Fragments arranged by size:
2028 1396 1264 1231 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2079 3238 3577 5920 6107 1700
Size: 110 269 1159 339 2343 187 2869
Fragments arranged by size:
2869 2343 1159 339 269 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7276
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7276
BssSI C'ACGA_G
Cuts at: 5336 6348 5336
Size: 1012 6264
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3637 794
Size: 2843 4433
BstBI TT'CG_AA
Cuts at: 6080 6080
Size: 7276
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7276
BstXI CCAn_nnnn'nTGG
Cuts at: 3188 3188
Size: 7276
BstYI r'GATC_y
Cuts at: 1972 2253 2451 3107 3169 4338 4606 4973 5358
Size: 281 198 656 62 1169 268 367 385
Cuts at: 5358 5375 6827 6913 6925 7271 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1169 656 385 367 346 281
268 198 86 62 17 12
Bsu36I CC'TnA_GG
Cuts at: 2553 2553
Size: 7276
BtgI C'CryG_G
Cuts at: 513 1065 2004 3899 513
Size: 552 939 1895 3890
Fragments arranged by size:
3890 1895 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3340 487
Size: 683 56 2114 4423
Fragments arranged by size:
4423 2114 683 56
BtsI GCAGTG_nn'
Cuts at: 2312 2661 3638 3843 4792 5586 5606 2312
Size: 349 977 205 949 794 20 3982
Fragments arranged by size:
3982 977 949 794 349 205 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7276
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3694 3729 529
Size: 35 3130 35 4076
Fragments arranged by size:
4076 3130 35 35
DraI TTT'AAA
Cuts at: 4580 4580
Size: 7276
DraIII CAC_nnn'GTG
Cuts at: 4450 4450
Size: 7276
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6283 809
Size: 507 4967 1802
Fragments arranged by size:
4967 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2446 2490
Size: 54 1425 50 275 18 291 325 44
Cuts at: 2490 2989 3262 4053 4524 4723 5612 7241 8
Size: 499 273 791 471 199 889 1629 43
Fragments arranged by size:
1629 1425 889 791 499 471 325 291
275 273 199 54 50 44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 4723 1537
Size: 275 1450 1461 4090
Fragments arranged by size:
4090 1461 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2819 3384 4169 5206 1353
Size: 1466 565 785 1037 3423
Fragments arranged by size:
3423 1466 1037 785 565
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5863 6237 6383 387
Size: 1064 138 4274 374 146 1280
Fragments arranged by size:
4274 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3943 4333 3943
Size: 390 6886
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2813 2912 2936 3099 4035 4319 5339
Size: 393 1181 99 24 163 936 284 1020
Cuts at: 5339 5936 6723 6991 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1181 1020 936 787 597 393 284
268 163 99 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2361 2057
Size: 304 6972
EcoO109I rG'GnC_Cy
Cuts at: 2015 2434 2788 4510 4519 2015
Size: 419 354 1722 9 4772
Fragments arranged by size:
4772 1722 419 354 9
EcoRI G'AATT_C
Cuts at: 4585 4963 4585
Size: 378 6898
FspI TGC'GCA
Cuts at: 4559 5782 4559
Size: 1223 6053
HaeII r_GCGC'y
Cuts at: 1678 2040 3052 3245 3748 6423 1678
Size: 362 1012 193 503 2675 2531
Fragments arranged by size:
2675 2531 1012 503 362 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3652 3684 5917 5949 5991
Size: 32 135 32 2080 32 2233 32 42
Cuts at: 5991 6023 1373
Size: 32 2626
Fragments arranged by size:
2626 2233 2080 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 2338 3461 4620 4866 669
Size: 988 281 271 129 1123 1159 246 3079
Fragments arranged by size:
3079 1159 1123 988 281 271 246 129
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7276
HpaI GTT'AAC
Cuts at: 4866 4866
Size: 7276
KasI G'GCGC_C
Cuts at: 3241 3241
Size: 7276
KpnI G_GTAC'C
Cuts at: 4601 4601
Size: 7276
MfeI C'AATT_G
Cuts at: 4875 4875
Size: 7276
MluI A'CGCG_T
Cuts at: 7152 7152
Size: 7276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3583 3705 6390 6574 1305
Size: 2278 122 2685 184 2007
Fragments arranged by size:
2685 2278 2007 184 122
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2123 2492 2991 4055 4526
Size: 54 1425 343 291 369 499 1064 471
Cuts at: 4526 10
Size: 2760
Fragments arranged by size:
2760 1425 1064 499 471 369 343 291
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 3836 4293 4467 5234 5593 5752
Size: 648 1359 1311 457 174 767 359 159
Cuts at: 5752 518
Size: 2042
Fragments arranged by size:
2042 1359 1311 767 648 457 359 174
159
MspA1I CmG'CkG
Cuts at: 1606 2030 2445 2572 2890 3418 4193 4354 4692
Size: 424 415 127 318 528 775 161 338
Cuts at: 4692 5369 6517 6762 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 775 677 528 424 415 338
318 245 161 127
NaeI GCC'GGC
Cuts at: 2081 3240 2081
Size: 1159 6117
NarI GG'CG_CC
Cuts at: 3242 3242
Size: 7276
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5785
Fragments arranged by size:
5785 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7276
NgoMIV G'CCGG_C
Cuts at: 2079 3238 2079
Size: 1159 6117
NheI G'CTAG_C
Cuts at: 1052 2401 2864 1052
Size: 1349 463 5464
Fragments arranged by size:
5464 1349 463
NotI GC'GGCC_GC
Cuts at: 4723 4723
Size: 7276
NspI r_CATG'y
Cuts at: 1132 1229 3310 4634 6179 1132
Size: 97 2081 1324 1545 2229
Fragments arranged by size:
2229 2081 1545 1324 97
PasI CC'CwG_GG
Cuts at: 1348 2357 1348
Size: 1009 6267
PciI A'CATG_T
Cuts at: 6175 6175
Size: 7276
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2397 2572 4450 4522 1108
Size: 1289 175 1878 72 3862
Fragments arranged by size:
3862 1878 1289 175 72
PfoI T'CCnGG_A
Cuts at: 3180 3180
Size: 7276
PmeI GTTT'AAAC
Cuts at: 4580 4580
Size: 7276
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2579 2611 3494 3518 3526 3550 5345 5377 6858
Size: 32 883 24 8 24 1795 32 1481
Cuts at: 6858 6890 2579
Size: 32 2965
Fragments arranged by size:
2965 1795 1481 883 32 32 32 24
24 8
PpuMI rG'GwC_Cy
Cuts at: 2015 4510 2015
Size: 2495 4781
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7276
PsiI TTA'TAA
Cuts at: 2673 4846 2673
Size: 2173 5103
PstI C_TGCA'G
Cuts at: 830 2058 3546 4628 830
Size: 1228 1488 1082 3478
Fragments arranged by size:
3478 1488 1228 1082
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7276
PvuII CAG'CTG
Cuts at: 2030 2890 3418 4193 4354 2030
Size: 860 528 775 161 4952
Fragments arranged by size:
4952 860 775 528 161
SacI G_AGCT'C
Cuts at: 3945 4335 3945
Size: 390 6886
SalI G'TCGA_C
Cuts at: 1936 2336 4618 1936
Size: 400 2282 4594
Fragments arranged by size:
4594 2282 400
SanDI GG'GwC_CC
Cuts at: 2015 4510 2015
Size: 2495 4781
SbfI CC_TGCA'GG
Cuts at: 4628 4628
Size: 7276
ScaI AGT'ACT
Cuts at: 5524 7236 5524
Size: 1712 5564
SexAI A'CCwGG_T
Cuts at: 4446 4446
Size: 7276
SfcI C'TryA_G
Cuts at: 826 1045 2054 3542 4624 5759 6440 6631 826
Size: 219 1009 1488 1082 1135 681 191 1471
Fragments arranged by size:
1488 1471 1135 1082 1009 681 219 191
SfoI GGC'GCC
Cuts at: 3243 3243
Size: 7276
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7276
SgrAI Cr'CCGG_yG
Cuts at: 1700 3577 1700
Size: 1877 5399
SmaI CCC'GGG
Cuts at: 1872 4603 1872
Size: 2731 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2957 3398 3521 3692 5380 6281
Size: 197 926 1014 441 123 171 1688 901
Cuts at: 6281 6543 820
Size: 262 1553
Fragments arranged by size:
1688 1553 1014 926 901 441 262 197
171 123
SnaBI TAC'GTA
Cuts at: 493 4369 5067 493
Size: 3876 698 2702
Fragments arranged by size:
3876 2702 698
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7276
SphI G_CATG'C
Cuts at: 1132 4634 1132
Size: 3502 3774
SspI AAT'ATT
Cuts at: 5 52 5200 5
Size: 47 5148 2081
Fragments arranged by size:
5148 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7276
StyI C'CwwG_G
Cuts at: 513 1065 2004 2463 2775 3432 4715 513
Size: 552 939 459 312 657 1283 3074
Fragments arranged by size:
3074 1283 939 657 552 459 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2822 3083 3301 4235 4239 5303 5320
Size: 1255 1242 261 218 934 4 1064 17
Cuts at: 5320 5473 5658 6103 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1242 1064 934 445 261 218
185 153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4566 5522 7234
Size: 274 80 33 51 1443 2589 956 1712
Cuts at: 7234 96
Size: 138
Fragments arranged by size:
2589 1712 1443 956 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2492 2524 2892 2924 7236 7268 2492
Size: 32 368 32 4312 32 2500
Fragments arranged by size:
4312 2500 368 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3676 1316
Size: 2360 4916
XbaI T'CTAG_A
Cuts at: 4612 4612
Size: 7276
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2500 2500
Size: 7276
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7276
XmaI C'CCGG_G
Cuts at: 1870 4601 1870
Size: 2731 4545
XmnI GAAnn'nnTTC
Cuts at: 3320 5405 3320
Size: 2085 5191
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5084 276
Size: 53 83 186 936 3550 2468
Fragments arranged by size:
3550 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BfrBI BsmBI BstZ17I EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI