(Circular) (Six-base) MAPSORT of: pFN21AB5068.seq Check: 1271 from: 1 to: 6346 pFN21AB5068 6346 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3023 3023 Size: 6346 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4156 278 Size: 53 83 186 936 2620 2468 Fragments arranged by size: 2620 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3689 1937 Size: 1752 4594 Acc65I G'GTAC_C Cuts at: 3667 3667 Size: 6346 AclI AA'CG_TT Cuts at: 1563 4473 4846 1563 Size: 2910 373 3063 Fragments arranged by size: 3063 2910 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2037 2045 2388 2976 4409 5793 6061 1632 Size: 405 8 343 588 1433 1384 268 1917 Fragments arranged by size: 1917 1433 1384 588 405 343 268 8 AflII C'TTAA_G Cuts at: 820 1017 2657 3383 820 Size: 197 1640 726 3783 Fragments arranged by size: 3783 1640 726 197 AflIII A'CryG_T Cuts at: 1203 5245 6222 1203 Size: 4042 977 1327 Fragments arranged by size: 4042 1327 977 AgeI A'CCGG_T Cuts at: 5177 5177 Size: 6346 AhdI GACnn_n'nnGTC Cuts at: 5075 5075 Size: 6346 AleI CACnn'nnGTG Cuts at: 1166 2861 1166 Size: 1695 4651 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2804 1184 Size: 1620 4726 AlwNI CAG_nnn'CTG Cuts at: 2317 2366 3424 4089 5661 2317 Size: 49 1058 665 1572 3002 Fragments arranged by size: 3002 1572 1058 665 49 ApaI G_GGCC'C Cuts at: 2158 2971 3003 3236 2158 Size: 813 32 233 5268 Fragments arranged by size: 5268 813 233 32 ApaLI G'TGCA_C Cuts at: 4403 5559 4403 Size: 1156 5190 ApoI r'AATT_y Cuts at: 1493 2735 3620 3655 3881 4033 6142 1493 Size: 1242 885 35 226 152 2109 1697 Fragments arranged by size: 2109 1697 1242 885 226 152 35 AseI AT'TA_AT Cuts at: 160 1032 4900 160 Size: 872 3868 1606 Fragments arranged by size: 3868 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 AvaI C'yCGr_G Cuts at: 1870 1943 2151 2334 3042 3671 1870 Size: 73 208 183 708 629 4545 Fragments arranged by size: 4545 708 629 208 183 73 AvrII C'CTAG_G Cuts at: 3407 3407 Size: 6346 BamHI G'GATC_C Cuts at: 3676 4043 3676 Size: 367 5979 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2895 3667 5122 618 Size: 325 217 627 1108 772 1455 1842 Fragments arranged by size: 1842 1455 1108 772 627 325 217 BanII G_rGCy'C Cuts at: 1391 2158 2168 2544 2971 3003 3236 3665 1391 Size: 767 10 376 427 32 233 429 4072 Fragments arranged by size: 4072 767 429 427 376 233 32 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2180 2376 2736 3405 3540 928 Size: 971 281 196 360 669 135 3734 Fragments arranged by size: 3734 971 669 360 281 196 135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3311 3345 4537 4571 1903 Size: 34 1374 34 1192 34 3678 Fragments arranged by size: 3678 1374 1192 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4238 5448 4238 Size: 1210 5136 BclI T'GATC_A Cuts at: 1548 1548 Size: 6346 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4957 136 Size: 107 122 71 631 605 3285 1525 Fragments arranged by size: 3285 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6341 6341 Size: 6346 BlpI GC'TnA_GC Cuts at: 3763 3763 Size: 6346 Bme1580I G_kGCm'C Cuts at: 1790 2158 2971 3003 3236 4407 5563 1790 Size: 368 813 32 233 1171 1156 2573 Fragments arranged by size: 2573 1171 1156 813 368 233 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6346 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2033 2966 4077 Size: 416 53 83 399 24 616 933 1111 Cuts at: 4077 5035 442 Size: 958 1753 Fragments arranged by size: 1753 1111 958 933 616 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6346 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2504 2875 3401 5006 1239 Size: 1265 371 526 1605 2579 Fragments arranged by size: 2579 1605 1265 526 371 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2528 2609 2744 4471 5336 5634 2528 Size: 81 135 1727 865 298 3240 Fragments arranged by size: 3240 1727 865 298 135 81 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5009 882 Size: 617 3510 2219 Fragments arranged by size: 3510 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4137 493 Size: 711 2933 2702 Fragments arranged by size: 2933 2702 711 BsaBI GATnn'nnATC Cuts at: 6340 6340 Size: 6346 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4153 4535 5170 275 Size: 53 83 186 936 2620 382 635 1451 Fragments arranged by size: 2620 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2233 3025 3258 4779 5177 5451 5598 6009 2233 Size: 792 233 1521 398 274 147 411 2570 Fragments arranged by size: 2570 1521 792 411 398 274 233 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6121 Fragments arranged by size: 6121 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2543 3221 3281 1186 Size: 1357 678 60 4251 Fragments arranged by size: 4251 1357 678 60 BseYI C'CCAG_C Cuts at: 2673 5549 6045 2673 Size: 2876 496 2974 Fragments arranged by size: 2974 2876 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5773 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2181 3796 4557 5585 1540 Size: 275 186 180 1615 761 1028 2301 Fragments arranged by size: 2301 1615 1028 761 275 186 180 BsiHKAI G_wGCw'C Cuts at: 2335 2544 2868 3078 4407 4492 5563 2335 Size: 209 324 210 1329 85 1071 3118 Fragments arranged by size: 3118 1329 1071 324 210 209 85 BsmI GAATG_Cn' Cuts at: 3370 3857 3950 3370 Size: 487 93 5766 Fragments arranged by size: 5766 487 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2158 2168 2335 2544 2868 2971 3003 Size: 399 368 10 167 209 324 103 32 Cuts at: 3003 3078 3236 3665 4407 4492 5563 1391 Size: 75 158 429 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 429 399 368 324 209 167 158 103 85 75 32 10 BspEI T'CCGG_A Cuts at: 3025 3258 6009 3025 Size: 233 2751 3362 Fragments arranged by size: 3362 2751 233 BspHI T'CATG_A Cuts at: 4235 5965 4235 Size: 1730 4616 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3023 3701 844 Size: 2179 678 3489 Fragments arranged by size: 3489 2179 678 BsrBI CCG'CTC Cuts at: 1394 1823 4233 1394 Size: 429 2410 3507 Fragments arranged by size: 3507 2410 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4841 5015 65 Size: 1183 3593 174 1396 Fragments arranged by size: 3593 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2820 4990 5177 1700 Size: 110 1010 2170 187 2869 Fragments arranged by size: 2869 2170 1010 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6346 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6346 BssSI C'ACGA_G Cuts at: 4406 5418 4406 Size: 1012 5334 BstAPI GCAn_nnn'nTGC Cuts at: 794 2209 2951 794 Size: 1415 742 4189 Fragments arranged by size: 4189 1415 742 BstBI TT'CG_AA Cuts at: 2031 5150 2031 Size: 3119 3227 BstEII G'GTnAC_C Cuts at: 1184 2650 1184 Size: 1466 4880 BstXI CCAn_nnnn'nTGG Cuts at: 2122 2284 2681 3122 2122 Size: 162 397 441 5346 Fragments arranged by size: 5346 441 397 162 BstYI r'GATC_y Cuts at: 1972 3676 4043 4428 4445 5897 5983 5995 6341 Size: 1704 367 385 17 1452 86 12 346 Cuts at: 6341 1972 Size: 1977 Fragments arranged by size: 1977 1704 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3102 3102 Size: 6346 BtgI C'CryG_G Cuts at: 513 1065 2004 3177 3508 513 Size: 552 939 1173 331 3351 Fragments arranged by size: 3351 1173 939 552 331 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5607 Fragments arranged by size: 5607 683 56 BtsI GCAGTG_nn' Cuts at: 2256 2882 3459 3590 3862 4656 4676 2256 Size: 626 577 131 272 794 20 3926 Fragments arranged by size: 3926 794 626 577 272 131 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6346 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6311 DraI TTT'AAA Cuts at: 3650 3650 Size: 6346 DraIII CAC_nnn'GTG Cuts at: 3064 3064 Size: 6346 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5353 809 Size: 507 4037 1802 Fragments arranged by size: 4037 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2349 3793 Size: 54 1425 50 275 18 348 171 1444 Cuts at: 3793 4682 6311 8 Size: 889 1629 43 Fragments arranged by size: 1629 1444 1425 889 348 275 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2178 3793 1537 Size: 275 366 1615 4090 Fragments arranged by size: 4090 1615 366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2532 3354 4276 1353 Size: 1179 822 922 3423 Fragments arranged by size: 3423 1179 922 822 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4933 5307 5453 387 Size: 1064 138 3344 374 146 1280 Fragments arranged by size: 3344 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2542 2542 Size: 6346 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2045 2388 2504 2875 2976 3401 Size: 393 405 8 343 116 371 101 425 Cuts at: 3401 4409 5006 5793 6061 1239 Size: 1008 597 787 268 1524 Fragments arranged by size: 1524 1008 787 597 425 405 393 371 343 268 116 101 8 EcoNI CCTnn'n_nnAGG Cuts at: 3556 3556 Size: 6346 EcoO109I rG'GnC_Cy Cuts at: 2140 2424 2967 2968 2999 3232 3233 3412 2140 Size: 284 543 1 31 233 1 179 5074 Fragments arranged by size: 5074 543 284 233 179 31 1 1 EcoRI G'AATT_C Cuts at: 3655 4033 3655 Size: 378 5968 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2528 3409 2528 Size: 881 5465 FspI TGC'GCA Cuts at: 3596 4852 3596 Size: 1256 5090 HaeII r_GCGC'y Cuts at: 1678 5493 1678 Size: 3815 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2395 2416 2448 3002 Size: 32 135 32 791 32 21 32 554 Cuts at: 3002 3034 4987 5019 5061 5093 1373 Size: 32 1953 32 42 32 2626 Fragments arranged by size: 2626 1953 791 554 135 42 32 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 2800 3690 3936 669 Size: 988 281 862 890 246 3079 Fragments arranged by size: 3079 988 890 862 281 246 HindIII A'AGCT_T Cuts at: 748 3380 748 Size: 2632 3714 HpaI GTT'AAC Cuts at: 3936 3936 Size: 6346 KpnI G_GTAC'C Cuts at: 3671 3671 Size: 6346 MfeI C'AATT_G Cuts at: 3945 3945 Size: 6346 MluI A'CGCG_T Cuts at: 6222 6222 Size: 6346 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5460 5644 1305 Size: 4155 184 2007 Fragments arranged by size: 4155 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2351 10 Size: 54 1425 343 519 4005 Fragments arranged by size: 4005 1425 519 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2861 4304 4663 4822 518 Size: 648 1695 1443 359 159 2042 Fragments arranged by size: 2042 1695 1443 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2206 2317 2363 2677 3421 3762 4439 5587 Size: 600 111 46 314 744 341 677 1148 Cuts at: 5587 5832 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 744 677 600 341 314 245 111 46 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4855 Fragments arranged by size: 4855 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6346 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6346 NotI GC'GGCC_GC Cuts at: 3793 3793 Size: 6346 NruI TCG'CGA Cuts at: 2089 2089 Size: 6346 NspI r_CATG'y Cuts at: 1132 1229 3704 5249 1132 Size: 97 2475 1545 2229 Fragments arranged by size: 2475 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6346 PciI A'CATG_T Cuts at: 5245 5245 Size: 6346 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6346 PfoI T'CCnGG_A Cuts at: 2103 2168 2103 Size: 65 6281 PmeI GTTT'AAAC Cuts at: 3650 3650 Size: 6346 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2247 2279 4415 4447 5928 5960 2247 Size: 32 2136 32 1481 32 2633 Fragments arranged by size: 2633 2136 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2140 2424 3412 2140 Size: 284 988 5074 Fragments arranged by size: 5074 988 284 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6346 PsiI TTA'TAA Cuts at: 3916 3916 Size: 6346 PspOMI G'GGCC_C Cuts at: 2154 2967 2999 3232 2154 Size: 813 32 233 5268 Fragments arranged by size: 5268 813 233 32 PstI C_TGCA'G Cuts at: 830 2205 3296 3698 830 Size: 1375 1091 402 3478 Fragments arranged by size: 3478 1375 1091 402 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6346 PvuII CAG'CTG Cuts at: 2206 2317 2363 2677 3421 2206 Size: 111 46 314 744 5131 Fragments arranged by size: 5131 744 314 111 46 SacI G_AGCT'C Cuts at: 2544 2544 Size: 6346 SalI G'TCGA_C Cuts at: 1936 3688 1936 Size: 1752 4594 SanDI GG'GwC_CC Cuts at: 2424 2424 Size: 6346 SapI GCTCTTCn'nnn_ Cuts at: 2532 3354 2532 Size: 822 5524 SbfI CC_TGCA'GG Cuts at: 3698 3698 Size: 6346 ScaI AGT'ACT Cuts at: 4594 6306 4594 Size: 1712 4634 SexAI A'CCwGG_T Cuts at: 3489 3489 Size: 6346 SfcI C'TryA_G Cuts at: 826 1045 2201 3292 3694 4829 5510 5701 826 Size: 219 1156 1091 402 1135 681 191 1471 Fragments arranged by size: 1471 1156 1135 1091 681 402 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6346 SmaI CCC'GGG Cuts at: 1872 3044 3673 1872 Size: 1172 629 4545 Fragments arranged by size: 4545 1172 629 SmlI C'TyrA_G Cuts at: 820 1017 1943 2543 2624 2657 2759 3383 4450 Size: 197 926 600 81 33 102 624 1067 Cuts at: 4450 5351 5613 820 Size: 901 262 1553 Fragments arranged by size: 1553 1067 926 901 624 600 262 197 102 81 33 SnaBI TAC'GTA Cuts at: 493 4137 493 Size: 3644 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6346 SphI G_CATG'C Cuts at: 1132 3704 1132 Size: 2572 3774 SspI AAT'ATT Cuts at: 5 52 4270 5 Size: 47 4218 2081 Fragments arranged by size: 4218 2081 47 StuI AGG'CCT Cuts at: 1517 2259 3017 3107 1517 Size: 742 758 90 4756 Fragments arranged by size: 4756 758 742 90 StyI C'CwwG_G Cuts at: 513 1065 2004 2277 2920 3407 3785 513 Size: 552 939 273 643 487 378 3074 Fragments arranged by size: 3074 939 643 552 487 378 273 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4373 4390 4543 4728 5173 325 Size: 1255 2793 17 153 185 445 1498 Fragments arranged by size: 2793 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4592 6304 96 Size: 274 80 33 51 1443 2615 1712 138 Fragments arranged by size: 2615 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6306 6338 6306 Size: 32 6314 Tth111I GACn'n_nGTC Cuts at: 1316 2375 2936 1316 Size: 1059 561 4726 Fragments arranged by size: 4726 1059 561 XbaI T'CTAG_A Cuts at: 2806 3682 2806 Size: 876 5470 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6346 XmaI C'CCGG_G Cuts at: 1870 3042 3671 1870 Size: 1172 629 4545 Fragments arranged by size: 4545 1172 629 XmnI GAAnn'nnTTC Cuts at: 3363 4475 3363 Size: 1112 5234 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4154 276 Size: 53 83 186 936 2620 2468 Fragments arranged by size: 2620 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI