Restriction map for
pFN21AB5017
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI Sau3AI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 242 CGCG CG^CG
AcyI 974 GACGCC G[GA]^CG[CT]C
AflIII 860 ACATGT A^C[GA][CT]GT
AlwNI 766 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 883 CCTCAGG CC^T[ACGT]AGG
BbvCI 201 CCTCAGC CCTCAGC(-5/-2)
BfaI 791 CTAG C^TAG
BglI 935 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 974 GACGCC G[GA]^CG[CT]C
BsaI 260 GAGACC GGTCTC(1/5)
BseRI 199 CTCCTC GAGGAG(10/8)
BsgI 89 GTGCAG GTGCAG(16/14)
BspLU11I 860 ACATGT A^CATGT
BstUI 242 CGCG CG^CG
Bsu36I 883 CCTCAGG CC^T[ACGT]AGG
Eco81I 883 CCTCAGG CC^T[ACGT]AGG
EcoT14I 959 CCTTGG C^C[AT][AT]GG
HgaI 974 GACGC GACGC(5/10)
Hin1I 974 GACGCC G[GA]^CG[CT]C
HincII 328 GTCAAC GT[CT]^[GA]AC
HindII 328 GTCAAC GT[CT]^[GA]AC
MaeI 791 CTAG C^TAG
MaeII 670 ACGT A^CGT
MslI 9 CACCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 242 CGCG CG^CG
NruI 241 TCGCGA TCG^CGA
NspI 860 ACATGT [GA]CATG^[CT]
PflMI 765 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 566 GGGACCT [GA]G^G[AT]CC[CT]
SapI 538 GAAGAGC GCTCTTC(1/4)
SfcI 75 CTATAG C^T[GA][CT]AG
StyI 959 CCTTGG C^C[AT][AT]GG
TaqI 32 TCGA T^CGA
TfiI 889 GATTC G^A[AT]TC
Tsp45I 163 GTCAC ^GT[GC]AC
TthHB8I 32 TCGA T^CGA
Van91I 765 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 390 GTAC GT^AC
456 GTAC
ApaI 508 GGGCCC GGGCC^C
560 GGGCCC
AvaII 567 GGACC G^G[AT]CC
900 GGACC
BsmAI 245 GAGAC GTCTC(1/5)
260 GAGAC
BssSI 424 CACGAG CACGAG(-5/-1)
13 CTCGTG
BstF5I 420 CATCC GGATG(2/0)
525 CATCC
CfoI 543 GCGC GCG^C
698 GCGC
EaeI 321 CGGCCA [CT]^GGCC[GA]
554 TGGCCG
EarI 832 CTCTTC CTCTTC(1/4)
538 GAAGAG
FokI 420 CATCC GGATG(9/13)
525 CATCC
HaeII 542 AGCGCC [GA]GCGC^[CT]
697 AGCGCC
HhaI 543 GCGC GCG^C
698 GCGC
HinP1I 543 GCGC G^CGC
698 GCGC
Ksp632I 832 CTCTTC CTCTTC(1/4)
538 GAAGAG
MaeIII 59 GTAAC ^GT[ACGT]AC
163 GTCAC
RsaI 390 GTAC GT^AC
456 GTAC
SacI 237 GAGCTC GAGCT^C
427 GAGCTC
SfaNI 66 GATGC GCATC(5/9)
419 GCATC
XcmI 647 CCACCGGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
761 CCAGCCAGGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AspHI AvaI BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PleI
Sau96I ScrFI SmaI SmlI TseI
TspRI XmaCI XmaI