(Circular) (Six-base) MAPSORT of: pFN21AB5017.seq Check: 5900 from: 1 to: 5719 pFN21AB5017 5719 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3529 278 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3062 1937 Size: 1125 4594 Acc65I G'GTAC_C Cuts at: 3040 3040 Size: 5719 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2562 2977 3782 5166 5434 1632 Size: 930 415 805 1384 268 1917 Fragments arranged by size: 1917 1384 930 805 415 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 2865 4618 5595 1203 Size: 1662 1753 977 1327 Fragments arranged by size: 1753 1662 1327 977 AgeI A'CCGG_T Cuts at: 4550 4550 Size: 5719 AhdI GACnn_n'nnGTC Cuts at: 4448 4448 Size: 5719 AleI CACnn'nnGTG Cuts at: 1166 2018 1166 Size: 852 4867 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2119 2822 1184 Size: 935 703 4081 Fragments arranged by size: 4081 935 703 AlwNI CAG_nnn'CTG Cuts at: 2776 3462 5034 2776 Size: 686 1572 3461 Fragments arranged by size: 3461 1572 686 ApaI G_GGCC'C Cuts at: 2517 2569 2517 Size: 52 5667 ApaLI G'TGCA_C Cuts at: 3776 4932 3776 Size: 1156 4563 ApoI r'AATT_y Cuts at: 1493 3028 3254 3406 5515 1493 Size: 1535 226 152 2109 1697 Fragments arranged by size: 2109 1697 1535 226 152 AseI AT'TA_AT Cuts at: 160 1032 4273 160 Size: 872 3241 1606 Fragments arranged by size: 3241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 2318 2568 2810 3044 1870 Size: 73 375 250 242 234 4545 Fragments arranged by size: 4545 375 250 242 234 73 BamHI G'GATC_C Cuts at: 3049 3416 3049 Size: 367 5352 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2129 2384 2741 3040 4495 Size: 325 217 627 342 255 357 299 1455 Cuts at: 4495 618 Size: 1842 Fragments arranged by size: 1842 1455 627 357 342 325 299 255 217 BanII G_rGCy'C Cuts at: 1391 2246 2436 2517 2569 2723 3038 1391 Size: 855 190 81 52 154 315 4072 Fragments arranged by size: 4072 855 315 190 154 81 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4748 BbvCI CC'TCA_GC Cuts at: 2207 2207 Size: 5719 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3910 3944 1903 Size: 34 1973 34 3678 Fragments arranged by size: 3678 1973 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3611 4821 3611 Size: 1210 4509 BclI T'GATC_A Cuts at: 1548 1548 Size: 5719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2946 4330 136 Size: 107 122 71 631 605 1274 1384 1525 Fragments arranged by size: 1525 1384 1274 631 605 122 107 71 BglII A'GATC_T Cuts at: 5714 5714 Size: 5719 BlpI GC'TnA_GC Cuts at: 3136 3136 Size: 5719 Bme1580I G_kGCm'C Cuts at: 1790 2132 2517 2557 2569 3780 4936 1790 Size: 342 385 40 12 1211 1156 2573 Fragments arranged by size: 2573 1211 1156 385 342 40 12 BmgBI CAC'GTC Cuts at: 1318 2676 1318 Size: 1358 4361 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2085 2622 3450 Size: 416 53 83 399 24 668 537 828 Cuts at: 3450 4408 442 Size: 958 1753 Fragments arranged by size: 1753 958 828 668 537 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2323 2582 2749 2914 4379 1239 Size: 1084 259 167 165 1465 2579 Fragments arranged by size: 2579 1465 1084 259 167 165 Bpu10I CC'TnA_GC Cuts at: 2207 2921 2207 Size: 714 5005 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2062 2201 2959 3844 4709 5007 2062 Size: 139 758 885 865 298 2774 Fragments arranged by size: 2774 885 865 758 298 139 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2259 4382 882 Size: 617 760 2123 2219 Fragments arranged by size: 2219 2123 760 617 BsaAI yAC'GTr Cuts at: 493 1204 3510 493 Size: 711 2306 2702 Fragments arranged by size: 2702 2306 711 BsaBI GATnn'nnATC Cuts at: 5713 5713 Size: 5719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2980 3526 3908 4543 Size: 53 83 186 936 1447 546 382 635 Cuts at: 4543 275 Size: 1451 Fragments arranged by size: 1451 1447 936 635 546 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4152 4550 4824 4971 5382 4152 Size: 398 274 147 411 4489 Fragments arranged by size: 4489 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5494 Fragments arranged by size: 5494 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 1186 Size: 1009 4710 BseYI C'CCAG_C Cuts at: 2177 2618 2882 4922 5418 2177 Size: 441 264 2040 496 2478 Fragments arranged by size: 2478 2040 496 441 264 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2115 1163 Size: 573 379 4767 Fragments arranged by size: 4767 573 379 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3169 3930 4958 1540 Size: 275 186 1168 761 1028 2301 Fragments arranged by size: 2301 1168 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2089 2246 2436 3780 3865 4936 2089 Size: 157 190 1344 85 1071 2872 Fragments arranged by size: 2872 1344 1071 190 157 85 BsmI GAATG_Cn' Cuts at: 3230 3323 3230 Size: 93 5626 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2089 2132 2246 2436 2517 2557 2569 Size: 399 299 43 114 190 81 40 12 Cuts at: 2569 2723 3038 3780 3865 4936 1391 Size: 154 315 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 315 299 190 154 114 85 81 43 40 12 BspEI T'CCGG_A Cuts at: 5382 5382 Size: 5719 BspHI T'CATG_A Cuts at: 3608 5338 3608 Size: 1730 3989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3074 844 Size: 2230 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2237 2271 2293 3606 1394 Size: 429 414 34 22 1313 3507 Fragments arranged by size: 3507 1313 429 414 34 22 BsrDI GCAATG_nn' Cuts at: 65 1248 4214 4388 65 Size: 1183 2966 174 1396 Fragments arranged by size: 2966 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4363 4550 1700 Size: 110 2553 187 2869 Fragments arranged by size: 2869 2553 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5719 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 2018 2429 3779 4791 2018 Size: 411 1350 1012 2946 Fragments arranged by size: 2946 1350 1012 411 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5719 BstBI TT'CG_AA Cuts at: 4523 4523 Size: 5719 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5719 BstYI r'GATC_y Cuts at: 1972 3049 3416 3801 3818 5270 5356 5368 5714 Size: 1077 367 385 17 1452 86 12 346 Cuts at: 5714 1972 Size: 1977 Fragments arranged by size: 1977 1452 1077 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2889 2889 Size: 5719 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5167 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4980 Fragments arranged by size: 4980 683 56 BtsI GCAGTG_nn' Cuts at: 2130 3235 4029 4049 2130 Size: 1105 794 20 3800 Fragments arranged by size: 3800 1105 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 3023 3023 Size: 5719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4726 809 Size: 507 3410 1802 Fragments arranged by size: 3410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2326 2559 3166 Size: 54 1425 50 275 18 496 233 607 Cuts at: 3166 4055 5684 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 607 496 275 233 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3166 1537 Size: 275 1354 4090 Fragments arranged by size: 4090 1354 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2538 2843 3649 1353 Size: 1185 305 806 3423 Fragments arranged by size: 3423 1185 806 305 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4306 4680 4826 387 Size: 1064 138 2717 374 146 1280 Fragments arranged by size: 2717 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2244 2434 2244 Size: 190 5529 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2323 2562 2582 2749 2914 2977 3782 Size: 393 691 239 20 167 165 63 805 Cuts at: 3782 4379 5166 5434 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 805 787 691 597 393 268 239 167 165 63 20 EcoO109I rG'GnC_Cy Cuts at: 2514 2565 2572 2514 Size: 51 7 5661 Fragments arranged by size: 5661 51 7 EcoRI G'AATT_C Cuts at: 3028 3406 3028 Size: 378 5341 FspI TGC'GCA Cuts at: 4225 4225 Size: 5719 HaeII r_GCGC'y Cuts at: 1678 2551 2706 4866 1678 Size: 873 155 2160 2531 Fragments arranged by size: 2531 2160 873 155 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2885 2917 2991 3023 4360 Size: 32 135 32 1313 32 74 32 1337 Cuts at: 4360 4392 4434 4466 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1337 1313 135 74 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2335 3063 3309 669 Size: 988 281 397 728 246 3079 Fragments arranged by size: 3079 988 728 397 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5719 HpaI GTT'AAC Cuts at: 3309 3309 Size: 5719 KpnI G_GTAC'C Cuts at: 3044 3044 Size: 5719 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4833 5017 1305 Size: 3528 184 2007 Fragments arranged by size: 3528 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3897 Fragments arranged by size: 3897 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2018 3677 4036 4195 518 Size: 648 852 1659 359 159 2042 Fragments arranged by size: 2042 1659 852 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2520 2582 2636 2648 3135 3812 4960 5205 Size: 914 62 54 12 487 677 1148 245 Cuts at: 5205 1606 Size: 2120 Fragments arranged by size: 2120 1148 914 677 487 245 62 54 12 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5167 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 3166 3166 Size: 5719 NruI TCG'CGA Cuts at: 2248 2248 Size: 5719 NspI r_CATG'y Cuts at: 1132 1229 2869 3077 4622 1132 Size: 97 1640 208 1545 2229 Fragments arranged by size: 2229 1640 1545 208 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5719 PciI A'CATG_T Cuts at: 2865 4618 2865 Size: 1753 3966 PflMI CCAn_nnn'nTGG Cuts at: 1108 2776 1108 Size: 1668 4051 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3788 3820 5301 5333 3788 Size: 32 1481 32 4174 Fragments arranged by size: 4174 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2572 2572 Size: 5719 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5719 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PspOMI G'GGCC_C Cuts at: 2513 2565 2513 Size: 52 5667 PstI C_TGCA'G Cuts at: 830 3071 830 Size: 2241 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 SacI G_AGCT'C Cuts at: 2246 2436 2246 Size: 190 5529 SalI G'TCGA_C Cuts at: 1936 3061 1936 Size: 1125 4594 SapI GCTCTTCn'nnn_ Cuts at: 2538 2538 Size: 5719 SbfI CC_TGCA'GG Cuts at: 3071 3071 Size: 5719 ScaI AGT'ACT Cuts at: 3967 5679 3967 Size: 1712 4007 SfcI C'TryA_G Cuts at: 826 1045 2080 3067 4202 4883 5074 826 Size: 219 1035 987 1135 681 191 1471 Fragments arranged by size: 1471 1135 1035 987 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5719 SmaI CCC'GGG Cuts at: 1872 2320 2570 2812 3046 1872 Size: 448 250 242 234 4545 Fragments arranged by size: 4545 448 250 242 234 SmlI C'TyrA_G Cuts at: 820 1017 1943 2041 2216 2974 3823 4724 4986 Size: 197 926 98 175 758 849 901 262 Cuts at: 4986 820 Size: 1553 Fragments arranged by size: 1553 926 901 849 758 262 197 175 98 SnaBI TAC'GTA Cuts at: 493 3510 493 Size: 3017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 3077 1132 Size: 1945 3774 SspI AAT'ATT Cuts at: 5 52 3643 5 Size: 47 3591 2081 Fragments arranged by size: 3591 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5719 StyI C'CwwG_G Cuts at: 513 1065 2964 3158 513 Size: 552 1899 194 3074 Fragments arranged by size: 3074 1899 552 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 3746 3763 3916 4101 4546 325 Size: 1255 741 1425 17 153 185 445 1498 Fragments arranged by size: 1498 1425 1255 741 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2460 3965 5677 Size: 274 80 33 51 1443 483 1505 1712 Cuts at: 5677 96 Size: 138 Fragments arranged by size: 1712 1505 1443 483 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2409 2441 5679 5711 2409 Size: 32 3238 32 2417 Fragments arranged by size: 3238 2417 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5719 XbaI T'CTAG_A Cuts at: 3055 3055 Size: 5719 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2659 2773 2659 Size: 114 5605 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5719 XmaI C'CCGG_G Cuts at: 1870 2318 2568 2810 3044 1870 Size: 448 250 242 234 4545 Fragments arranged by size: 4545 448 250 242 234 XmnI GAAnn'nnTTC Cuts at: 3848 3848 Size: 5719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3527 276 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI