(Circular) (Six-base) MAPSORT of: pFN21AB5014.seq Check: 8191 from: 1 to: 5956 pFN21AB5014 5956 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2067 3299 1937 Size: 130 1232 4594 Fragments arranged by size: 4594 1232 130 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4019 5403 5671 1632 Size: 2387 1384 268 1917 Fragments arranged by size: 2387 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3104 820 Size: 197 2087 3672 Fragments arranged by size: 3672 2087 197 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2483 1184 Size: 1299 4657 AlwNI CAG_nnn'CTG Cuts at: 2086 2275 3699 5271 2086 Size: 189 1424 1572 2771 Fragments arranged by size: 2771 1572 1424 189 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 2147 3265 3491 3643 5752 1493 Size: 654 1118 226 152 2109 1697 Fragments arranged by size: 2109 1697 1118 654 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 2720 2753 2894 3281 1870 Size: 73 777 33 141 387 4545 Fragments arranged by size: 4545 777 387 141 73 33 BamHI G'GATC_C Cuts at: 2632 2871 3286 3653 2632 Size: 239 415 367 4935 Fragments arranged by size: 4935 415 367 239 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2423 2975 3277 4732 618 Size: 325 217 627 636 552 302 1455 1842 Fragments arranged by size: 1842 1455 636 627 552 325 302 217 BanII G_rGCy'C Cuts at: 1391 3275 1391 Size: 1884 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2601 2661 3126 928 Size: 971 702 60 465 3758 Fragments arranged by size: 3758 971 702 465 60 BbvCI CC'TCA_GC Cuts at: 3021 3021 Size: 5956 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4147 4181 1903 Size: 34 50 34 2126 34 3678 Fragments arranged by size: 3678 2126 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 2472 1548 Size: 924 5032 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 4567 136 Size: 107 122 71 631 605 368 2527 1525 Fragments arranged by size: 2527 1525 631 605 368 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2867 4017 5173 1790 Size: 1077 1150 1156 2573 Fragments arranged by size: 2573 1156 1150 1077 BmgBI CAC'GTC Cuts at: 1318 3042 1318 Size: 1724 4232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2668 2719 2770 Size: 416 53 83 399 24 1251 51 51 Cuts at: 2770 2789 2907 3687 4645 442 Size: 19 118 780 958 1753 Fragments arranged by size: 1753 1251 958 780 416 399 118 83 53 51 51 24 19 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2602 2602 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4616 1239 Size: 3377 2579 Bpu10I CC'TnA_GC Cuts at: 3021 3021 Size: 5956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4619 882 Size: 617 3120 2219 Fragments arranged by size: 3120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3763 4145 4780 275 Size: 53 83 186 936 2230 382 635 1451 Fragments arranged by size: 2230 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2650 4389 4787 5061 5208 5619 2650 Size: 1739 398 274 147 411 2987 Fragments arranged by size: 2987 1739 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5731 Fragments arranged by size: 5731 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2198 3038 3107 3125 3152 3155 3158 Size: 841 171 840 69 18 27 3 3 Cuts at: 3158 3203 3212 3233 1186 Size: 45 9 21 3909 Fragments arranged by size: 3909 841 840 171 69 45 27 21 18 9 3 3 BseYI C'CCAG_C Cuts at: 2944 5159 5655 2944 Size: 2215 496 3245 Fragments arranged by size: 3245 2215 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2303 1163 Size: 573 567 4816 Fragments arranged by size: 4816 573 567 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4017 4102 5173 4017 Size: 85 1071 4800 Fragments arranged by size: 4800 1071 85 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 BsmBI CGTCTCn'nnnn_ Cuts at: 2088 3032 2088 Size: 944 5012 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2867 3275 4017 4102 5173 1391 Size: 399 1077 408 742 85 1071 2174 Fragments arranged by size: 2174 1077 1071 742 408 399 85 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2953 3311 844 Size: 2109 358 3489 Fragments arranged by size: 3489 2109 358 BsrBI CCG'CTC Cuts at: 1394 1823 2668 3843 1394 Size: 429 845 1175 3507 Fragments arranged by size: 3507 1175 845 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4451 4625 65 Size: 1183 3203 174 1396 Fragments arranged by size: 3203 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4600 4787 1700 Size: 110 2790 187 2869 Fragments arranged by size: 2869 2790 187 110 BsrGI T'GTAC_A Cuts at: 96 2517 96 Size: 2421 3535 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 4016 5028 4016 Size: 1012 4944 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstYI r'GATC_y Cuts at: 1972 2632 2871 3286 3653 4038 4055 5507 5593 Size: 660 239 415 367 385 17 1452 86 Cuts at: 5593 5605 5951 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 660 415 385 367 346 239 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2703 2703 Size: 5956 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5404 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5217 Fragments arranged by size: 5217 683 56 BtsI GCAGTG_nn' Cuts at: 3472 4266 4286 3472 Size: 794 20 5142 Fragments arranged by size: 5142 794 20 ClaI AT'CG_AT Cuts at: 1554 2679 1554 Size: 1125 4831 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2238 2534 Size: 54 1425 50 275 18 216 192 296 Cuts at: 2534 3235 3403 4292 5921 8 Size: 701 168 889 1629 43 Fragments arranged by size: 1629 1425 889 701 296 275 216 192 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2763 3886 1353 Size: 1410 1123 3423 Fragments arranged by size: 3423 1410 1123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4543 4917 5063 387 Size: 1064 138 2954 374 146 1280 Fragments arranged by size: 2954 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4019 4616 5403 5671 1239 Size: 393 2387 597 787 268 1524 Fragments arranged by size: 2387 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2960 2960 Size: 5956 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 5103 1678 Size: 3425 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2617 2649 2668 2700 4597 Size: 32 135 32 1045 32 19 32 1897 Cuts at: 4597 4629 4671 4703 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1897 1045 135 42 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4134 Fragments arranged by size: 4134 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3914 4273 4432 518 Size: 648 2748 359 159 2042 Fragments arranged by size: 2748 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2112 3372 4049 5197 5442 1606 Size: 417 89 1260 677 1148 245 2120 Fragments arranged by size: 2120 1260 1148 677 417 245 89 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5404 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 2371 3314 4859 1132 Size: 97 1142 943 1545 2229 Fragments arranged by size: 2229 1545 1142 943 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2275 1108 Size: 1167 4789 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2798 2830 2798 Size: 32 5924 PstI C_TGCA'G Cuts at: 830 3308 830 Size: 2478 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SfcI C'TryA_G Cuts at: 826 1045 3304 4439 5120 5311 826 Size: 219 2259 1135 681 191 1471 Fragments arranged by size: 2259 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2040 2040 Size: 5956 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3104 4060 4961 5223 820 Size: 197 926 1161 956 901 262 1553 Fragments arranged by size: 1553 1161 956 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 2589 3243 3395 513 Size: 552 1524 654 152 3074 Fragments arranged by size: 3074 1524 654 552 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2632 2892 3983 4000 4153 4338 4783 Size: 1255 1052 260 1091 17 153 185 445 Cuts at: 4783 325 Size: 1498 Fragments arranged by size: 1498 1255 1091 1052 445 260 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2517 2814 4202 Size: 274 80 33 51 1443 540 297 1388 Cuts at: 4202 5914 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1388 540 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2768 2800 5916 5948 2768 Size: 32 3116 32 2776 Fragments arranged by size: 3116 2776 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3250 3250 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI