(Circular) (Six-base) MAPSORT of: pFN21AB5006.seq Check: 7808 from: 1 to: 6082 pFN21AB5006 6082 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2330 2330 Size: 6082 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3892 278 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3425 1937 Size: 1488 4594 Acc65I G'GTAC_C Cuts at: 3403 3403 Size: 6082 AclI AA'CG_TT Cuts at: 1563 4209 4582 1563 Size: 2646 373 3063 Fragments arranged by size: 3063 2646 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2126 4145 5529 5797 1632 Size: 494 2019 1384 268 1917 Fragments arranged by size: 2019 1917 1384 494 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5885 AflIII A'CryG_T Cuts at: 1203 4981 5958 1203 Size: 3778 977 1327 Fragments arranged by size: 3778 1327 977 AgeI A'CCGG_T Cuts at: 4913 4913 Size: 6082 AhdI GACnn_n'nnGTC Cuts at: 4811 4811 Size: 6082 AleI CACnn'nnGTG Cuts at: 1166 2819 1166 Size: 1653 4429 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6082 AlwNI CAG_nnn'CTG Cuts at: 3825 5397 3825 Size: 1572 4510 ApaLI G'TGCA_C Cuts at: 4139 5295 4139 Size: 1156 4926 ApoI r'AATT_y Cuts at: 1493 2231 2788 3391 3617 3769 5878 1493 Size: 738 557 603 226 152 2109 1697 Fragments arranged by size: 2109 1697 738 603 557 226 152 AseI AT'TA_AT Cuts at: 160 1032 4636 160 Size: 872 3604 1606 Fragments arranged by size: 3604 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 AvaI C'yCGr_G Cuts at: 1870 1943 3219 3407 1870 Size: 73 1276 188 4545 Fragments arranged by size: 4545 1276 188 73 BamHI G'GATC_C Cuts at: 3201 3412 3779 3201 Size: 211 367 5504 Fragments arranged by size: 5504 367 211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2050 3403 4858 618 Size: 325 217 627 263 1353 1455 1842 Fragments arranged by size: 1842 1455 1353 627 325 263 217 BanII G_rGCy'C Cuts at: 1391 2568 3401 1391 Size: 1177 833 4072 Fragments arranged by size: 4072 1177 833 BbeI G_GCGC'C Cuts at: 2054 2054 Size: 6082 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5111 BbvCI CC'TCA_GC Cuts at: 3261 3261 Size: 6082 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4273 4307 1903 Size: 34 2336 34 3678 Fragments arranged by size: 3678 2336 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3974 5184 3974 Size: 1210 4872 BclI T'GATC_A Cuts at: 1548 1548 Size: 6082 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3095 4693 136 Size: 107 122 71 631 605 1423 1598 1525 Fragments arranged by size: 1598 1525 1423 631 605 122 107 71 BglII A'GATC_T Cuts at: 2425 6077 2425 Size: 3652 2430 BlpI GC'TnA_GC Cuts at: 2490 2727 3499 2490 Size: 237 772 5073 Fragments arranged by size: 5073 772 237 Bme1580I G_kGCm'C Cuts at: 1790 2129 2837 4143 5299 1790 Size: 339 708 1306 1156 2573 Fragments arranged by size: 2573 1306 1156 708 339 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6082 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3813 4771 442 Size: 416 53 83 399 24 2396 958 1753 Fragments arranged by size: 2396 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6082 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3310 3378 3310 Size: 68 6014 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2194 2636 4742 1239 Size: 955 442 2106 2579 Fragments arranged by size: 2579 2106 955 442 Bpu10I CC'TnA_GC Cuts at: 3261 3261 Size: 6082 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 2735 4207 5072 5370 2651 Size: 84 1472 865 298 3363 Fragments arranged by size: 3363 1472 865 298 84 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3294 4745 882 Size: 617 1795 1451 2219 Fragments arranged by size: 2219 1795 1451 617 BsaAI yAC'GTr Cuts at: 493 1204 3873 493 Size: 711 2669 2702 Fragments arranged by size: 2702 2669 711 BsaBI GATnn'nnATC Cuts at: 6076 6076 Size: 6082 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2051 3889 4271 4906 Size: 53 83 186 936 518 1838 382 635 Cuts at: 4906 275 Size: 1451 Fragments arranged by size: 1838 1451 936 635 518 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4515 4913 5187 5334 5745 4515 Size: 398 274 147 411 4852 Fragments arranged by size: 4852 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2219 2249 1178 Size: 30 165 30 816 30 5011 Fragments arranged by size: 5011 816 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 3278 3281 3344 1186 Size: 844 1248 3 63 3924 Fragments arranged by size: 3924 1248 844 63 3 BseYI C'CCAG_C Cuts at: 2672 3177 5285 5781 2672 Size: 505 2108 496 2973 Fragments arranged by size: 2973 2108 505 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5509 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 3532 4293 5321 1540 Size: 275 186 30 1501 761 1028 2301 Fragments arranged by size: 2301 1501 1028 761 275 186 30 BsiHKAI G_wGCw'C Cuts at: 2568 2816 4143 4228 5299 2568 Size: 248 1327 85 1071 3351 Fragments arranged by size: 3351 1327 1071 248 85 BsmI GAATG_Cn' Cuts at: 3593 3686 3593 Size: 93 5989 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2568 2816 2837 3401 4143 4228 Size: 399 339 439 248 21 564 742 85 Cuts at: 4228 5299 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 564 439 399 339 248 85 21 BspEI T'CCGG_A Cuts at: 5745 5745 Size: 6082 BspHI T'CATG_A Cuts at: 3971 5701 3971 Size: 1730 4352 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2330 3437 844 Size: 1486 1107 3489 Fragments arranged by size: 3489 1486 1107 BsrBI CCG'CTC Cuts at: 1394 1823 2037 2830 3969 1394 Size: 429 214 793 1139 3507 Fragments arranged by size: 3507 1139 793 429 214 BsrDI GCAATG_nn' Cuts at: 65 1248 2277 4577 4751 65 Size: 1183 1029 2300 174 1396 Fragments arranged by size: 2300 1396 1183 1029 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4726 4913 1700 Size: 110 2916 187 2869 Fragments arranged by size: 2916 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6082 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6082 BssSI C'ACGA_G Cuts at: 4142 5154 4142 Size: 1012 5070 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6082 BstBI TT'CG_AA Cuts at: 4886 4886 Size: 6082 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6082 BstXI CCAn_nnnn'nTGG Cuts at: 2177 2299 2177 Size: 122 5960 BstYI r'GATC_y Cuts at: 1972 2425 2599 3201 3412 3779 4164 4181 5633 Size: 453 174 602 211 367 385 17 1452 Cuts at: 5633 5719 5731 6077 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 602 453 385 367 346 211 174 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3295 3295 Size: 6082 BtgI C'CryG_G Cuts at: 513 1065 2061 513 Size: 552 996 4534 Fragments arranged by size: 4534 996 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5343 Fragments arranged by size: 5343 683 56 BtsI GCAGTG_nn' Cuts at: 2695 3598 4392 4412 2695 Size: 903 794 20 4365 Fragments arranged by size: 4365 903 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6082 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6047 DraI TTT'AAA Cuts at: 3386 3386 Size: 6082 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5089 809 Size: 507 3773 1802 Fragments arranged by size: 3773 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2979 3096 3529 Size: 54 1425 50 275 18 1149 117 433 Cuts at: 3529 4418 6047 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1149 889 433 275 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3529 1537 Size: 275 1717 4090 Fragments arranged by size: 4090 1717 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2430 2790 3077 3332 4012 1353 Size: 1077 360 287 255 680 3423 Fragments arranged by size: 3423 1077 680 360 287 255 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2463 3065 4669 5043 5189 387 Size: 1064 138 874 602 1604 374 146 1280 Fragments arranged by size: 1604 1280 1064 874 602 374 146 138 EcoICRI GAG'CTC Cuts at: 2566 2566 Size: 6082 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2194 2636 4145 4742 5529 5797 Size: 393 494 68 442 1509 597 787 268 Cuts at: 5797 1239 Size: 1524 Fragments arranged by size: 1524 1509 787 597 494 442 393 268 68 EcoO109I rG'GnC_Cy Cuts at: 2950 3037 3067 3139 2950 Size: 87 30 72 5893 Fragments arranged by size: 5893 87 72 30 EcoRI G'AATT_C Cuts at: 2788 3391 3769 2788 Size: 603 378 5101 Fragments arranged by size: 5101 603 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3088 3088 Size: 6082 FspI TGC'GCA Cuts at: 4588 4588 Size: 6082 HaeII r_GCGC'y Cuts at: 1678 2054 5229 1678 Size: 376 3175 2531 Fragments arranged by size: 3175 2531 376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2061 2093 2151 2183 2219 Size: 32 135 32 489 32 58 32 36 Cuts at: 2219 2251 4723 4755 4797 4829 1373 Size: 32 2472 32 42 32 2626 Fragments arranged by size: 2626 2472 489 135 58 42 36 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3426 3672 669 Size: 988 281 1488 246 3079 Fragments arranged by size: 3079 1488 988 281 246 HindIII A'AGCT_T Cuts at: 748 2868 748 Size: 2120 3962 HpaI GTT'AAC Cuts at: 3672 3672 Size: 6082 KasI G'GCGC_C Cuts at: 2050 2050 Size: 6082 KpnI G_GTAC'C Cuts at: 3407 3407 Size: 6082 MfeI C'AATT_G Cuts at: 3681 3681 Size: 6082 MluI A'CGCG_T Cuts at: 5958 5958 Size: 6082 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5196 5380 1305 Size: 3891 184 2007 Fragments arranged by size: 3891 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2981 10 Size: 54 1425 343 1149 3111 Fragments arranged by size: 3111 1425 1149 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 2819 3320 4040 4399 4558 518 Size: 648 1131 522 501 720 359 159 2042 Fragments arranged by size: 2042 1131 720 648 522 501 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2560 3181 3199 3498 4175 5323 5568 Size: 450 504 621 18 299 677 1148 245 Cuts at: 5568 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 621 504 450 299 245 18 NarI GG'CG_CC Cuts at: 2051 2051 Size: 6082 NcoI C'CATG_G Cuts at: 513 1065 2061 513 Size: 552 996 4534 Fragments arranged by size: 4534 996 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6082 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6082 NotI GC'GGCC_GC Cuts at: 3529 3529 Size: 6082 NspI r_CATG'y Cuts at: 1132 1229 3440 4985 1132 Size: 97 2211 1545 2229 Fragments arranged by size: 2229 2211 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6082 PciI A'CATG_T Cuts at: 4981 4981 Size: 6082 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6082 PmeI GTTT'AAAC Cuts at: 3386 3386 Size: 6082 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4151 4183 5664 5696 4151 Size: 32 1481 32 4537 Fragments arranged by size: 4537 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3139 3139 Size: 6082 PshAI GACnn'nnGTC Cuts at: 1613 2211 1613 Size: 598 5484 PsiI TTA'TAA Cuts at: 3652 3652 Size: 6082 PstI C_TGCA'G Cuts at: 830 3147 3434 830 Size: 2317 287 3478 Fragments arranged by size: 3478 2317 287 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6082 PvuII CAG'CTG Cuts at: 3181 3181 Size: 6082 SacI G_AGCT'C Cuts at: 2568 2568 Size: 6082 SalI G'TCGA_C Cuts at: 1936 3424 1936 Size: 1488 4594 SapI GCTCTTCn'nnn_ Cuts at: 3332 3332 Size: 6082 SbfI CC_TGCA'GG Cuts at: 3434 3434 Size: 6082 ScaI AGT'ACT Cuts at: 4330 6042 4330 Size: 1712 4370 SfcI C'TryA_G Cuts at: 826 1045 2876 2999 3143 3430 4565 5246 5437 Size: 219 1831 123 144 287 1135 681 191 Cuts at: 5437 826 Size: 1471 Fragments arranged by size: 1831 1471 1135 681 287 219 191 144 123 SfiI GGCCn_nnn'nGGCC Cuts at: 3095 3095 Size: 6082 SfoI GGC'GCC Cuts at: 2052 2052 Size: 6082 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6082 SmaI CCC'GGG Cuts at: 1872 3221 3409 1872 Size: 1349 188 4545 Fragments arranged by size: 4545 1349 188 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 2750 4186 5087 5349 820 Size: 197 926 723 84 1436 901 262 1553 Fragments arranged by size: 1553 1436 926 901 723 262 197 84 SnaBI TAC'GTA Cuts at: 493 3873 493 Size: 3380 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6082 SphI G_CATG'C Cuts at: 1132 3440 1132 Size: 2308 3774 SspI AAT'ATT Cuts at: 5 52 4006 5 Size: 47 3954 2081 Fragments arranged by size: 3954 2081 47 StuI AGG'CCT Cuts at: 1517 3005 1517 Size: 1488 4594 StyI C'CwwG_G Cuts at: 513 1065 2061 2769 3279 3521 513 Size: 552 996 708 510 242 3074 Fragments arranged by size: 3074 996 708 552 510 242 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2045 2172 4109 4126 4279 4464 4909 Size: 1255 465 127 1937 17 153 185 445 Cuts at: 4909 325 Size: 1498 Fragments arranged by size: 1937 1498 1255 465 445 185 153 127 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2841 4328 6040 Size: 274 80 33 51 1443 864 1487 1712 Cuts at: 6040 96 Size: 138 Fragments arranged by size: 1712 1487 1443 864 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6042 6074 6042 Size: 32 6050 Tth111I GACn'n_nGTC Cuts at: 1316 2159 1316 Size: 843 5239 XbaI T'CTAG_A Cuts at: 3418 3418 Size: 6082 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2068 2296 2632 3178 2068 Size: 228 336 546 4972 Fragments arranged by size: 4972 546 336 228 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6082 XmaI C'CCGG_G Cuts at: 1870 3219 3407 1870 Size: 1349 188 4545 Fragments arranged by size: 4545 1349 188 XmnI GAAnn'nnTTC Cuts at: 4211 4211 Size: 6082 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3890 276 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI