(Circular) (Six-base) MAPSORT of: pFN21AB4995.seq Check: 2605 from: 1 to: 6727 pFN21AB4995 6727 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2644 2644 Size: 6727 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4537 278 Size: 53 83 186 936 3001 2468 Fragments arranged by size: 3001 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2055 4070 1937 Size: 118 2015 4594 Fragments arranged by size: 4594 2015 118 Acc65I G'GTAC_C Cuts at: 4048 4048 Size: 6727 AclI AA'CG_TT Cuts at: 1563 4854 5227 1563 Size: 3291 373 3063 Fragments arranged by size: 3291 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4790 6174 6442 1632 Size: 3158 1384 268 1917 Fragments arranged by size: 3158 1917 1384 268 AfeI AGC'GCT Cuts at: 2348 2348 Size: 6727 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6530 AflIII A'CryG_T Cuts at: 1203 4024 5626 6603 1203 Size: 2821 1602 977 1327 Fragments arranged by size: 2821 1602 1327 977 AgeI A'CCGG_T Cuts at: 3357 5558 3357 Size: 2201 4526 AhdI GACnn_n'nnGTC Cuts at: 5456 5456 Size: 6727 AleI CACnn'nnGTG Cuts at: 1166 3333 1166 Size: 2167 4560 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6727 AlwNI CAG_nnn'CTG Cuts at: 2125 2401 3032 3495 3704 4470 6042 2125 Size: 276 631 463 209 766 1572 2810 Fragments arranged by size: 2810 1572 766 631 463 276 209 ApaI G_GGCC'C Cuts at: 3399 3399 Size: 6727 ApaLI G'TGCA_C Cuts at: 2153 4784 5940 2153 Size: 2631 1156 2940 Fragments arranged by size: 2940 2631 1156 ApoI r'AATT_y Cuts at: 1493 4036 4262 4414 6523 1493 Size: 2543 226 152 2109 1697 Fragments arranged by size: 2543 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5281 160 Size: 872 4249 1606 Fragments arranged by size: 4249 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6727 AvaI C'yCGr_G Cuts at: 1870 1943 2791 2962 3103 3163 3345 4052 1870 Size: 73 848 171 141 60 182 707 4545 Fragments arranged by size: 4545 848 707 182 171 141 73 60 BamHI G'GATC_C Cuts at: 4057 4424 4057 Size: 367 6360 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3465 4048 5503 618 Size: 325 217 627 1678 583 1455 1842 Fragments arranged by size: 1842 1678 1455 627 583 325 217 BanII G_rGCy'C Cuts at: 1391 2192 3344 3399 3599 4046 1391 Size: 801 1152 55 200 447 4072 Fragments arranged by size: 4072 1152 801 447 200 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3846 928 Size: 971 1947 3809 Fragments arranged by size: 3809 1947 971 BbvCI CC'TCA_GC Cuts at: 2840 3503 2840 Size: 663 6064 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2000 2034 2035 2069 2234 2268 4918 Size: 34 63 34 1 34 165 34 2650 Cuts at: 4918 4952 1903 Size: 34 3678 Fragments arranged by size: 3678 2650 165 63 34 34 34 34 34 1 BciVI GTATCCnnnnn_n' Cuts at: 4619 5829 4619 Size: 1210 5517 BclI T'GATC_A Cuts at: 1548 1548 Size: 6727 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3379 5338 136 Size: 107 122 71 631 605 1707 1959 1525 Fragments arranged by size: 1959 1707 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2409 2662 6722 2409 Size: 253 4060 2414 Fragments arranged by size: 4060 2414 253 BlpI GC'TnA_GC Cuts at: 4144 4144 Size: 6727 Bme1580I G_kGCm'C Cuts at: 1790 2157 2257 3399 4788 5944 1790 Size: 367 100 1142 1389 1156 2573 Fragments arranged by size: 2573 1389 1156 1142 367 100 BmgBI CAC'GTC Cuts at: 1318 2620 2632 1318 Size: 1302 12 5413 Fragments arranged by size: 5413 1302 12 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2113 4458 5416 Size: 416 53 83 399 24 696 2345 958 Cuts at: 5416 442 Size: 1753 Fragments arranged by size: 2345 1753 958 696 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6727 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2602 2944 2956 2602 Size: 342 12 6373 Fragments arranged by size: 6373 342 12 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 3157 3169 3181 5387 1239 Size: 1184 734 12 12 2206 2579 Fragments arranged by size: 2579 2206 1184 734 12 12 Bpu10I CC'TnA_GC Cuts at: 2840 3503 2840 Size: 663 6064 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2789 3976 4852 5717 6015 2789 Size: 1187 876 865 298 3501 Fragments arranged by size: 3501 1187 876 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2437 5390 882 Size: 617 938 2953 2219 Fragments arranged by size: 2953 2219 938 617 BsaAI yAC'GTr Cuts at: 493 1204 4518 493 Size: 711 3314 2702 Fragments arranged by size: 3314 2702 711 BsaBI GATnn'nnATC Cuts at: 6721 6721 Size: 6727 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2797 2809 3722 4534 Size: 53 83 186 936 1264 12 913 812 Cuts at: 4534 4916 5551 275 Size: 382 635 1451 Fragments arranged by size: 1451 1264 936 913 812 635 382 186 83 53 12 BsaWI w'CCGG_w Cuts at: 2721 3357 5160 5558 5832 5979 6390 2721 Size: 636 1803 398 274 147 411 3058 Fragments arranged by size: 3058 1803 636 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2546 2576 3236 3266 3347 Size: 30 165 30 1143 30 660 30 81 Cuts at: 3347 3377 1178 Size: 30 4528 Fragments arranged by size: 4528 1143 660 165 81 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 2567 2570 2573 2588 2591 2750 2786 Size: 1219 162 3 3 15 3 159 36 Cuts at: 2786 2789 2987 3029 3124 3127 3130 3133 3136 Size: 3 198 42 95 3 3 3 3 Cuts at: 3136 3139 3142 1186 Size: 3 3 4771 Fragments arranged by size: 4771 1219 198 162 159 95 42 36 15 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2113 2447 2864 3084 3551 3557 5930 6426 2113 Size: 334 417 220 467 6 2373 496 2414 Fragments arranged by size: 2414 2373 496 467 417 334 220 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2384 1163 Size: 573 648 5506 Fragments arranged by size: 5506 648 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2571 3241 3454 3639 4177 4938 Size: 275 186 570 670 213 185 538 761 Cuts at: 4938 5966 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 670 570 538 275 213 186 185 BsiHKAI G_wGCw'C Cuts at: 2157 4788 4873 5944 2157 Size: 2631 85 1071 2940 Fragments arranged by size: 2940 2631 1071 85 BsmI GAATG_Cn' Cuts at: 4238 4331 4238 Size: 93 6634 BsmBI CGTCTCn'nnnn_ Cuts at: 2074 3342 2074 Size: 1268 5459 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2192 2257 3344 3399 3599 4046 Size: 399 367 35 65 1087 55 200 447 Cuts at: 4046 4788 4873 5944 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1087 1071 742 447 399 367 200 85 65 55 35 BspEI T'CCGG_A Cuts at: 2721 6390 2721 Size: 3669 3058 BspHI T'CATG_A Cuts at: 4616 6346 4616 Size: 1730 4997 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2119 2644 3688 4082 844 Size: 1275 525 1044 394 3489 Fragments arranged by size: 3489 1275 1044 525 394 BsrBI CCG'CTC Cuts at: 1394 1823 2032 2077 2802 2940 2946 2952 3132 Size: 429 209 45 725 138 6 6 180 Cuts at: 3132 3183 3207 3252 3264 3276 3282 3288 3369 Size: 51 24 45 12 12 6 6 81 Cuts at: 3369 3420 3826 3835 3883 3942 4614 1394 Size: 51 406 9 48 59 672 3507 Fragments arranged by size: 3507 725 672 429 406 209 180 138 81 59 51 51 48 45 45 24 12 12 9 6 6 6 6 BsrDI GCAATG_nn' Cuts at: 65 1248 5222 5396 65 Size: 1183 3974 174 1396 Fragments arranged by size: 3974 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2378 3321 3357 3634 3930 5371 5558 Size: 110 568 943 36 277 296 1441 187 Cuts at: 5558 1700 Size: 2869 Fragments arranged by size: 2869 1441 943 568 296 277 187 110 36 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6727 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6727 BssSI C'ACGA_G Cuts at: 2012 4787 5799 2012 Size: 2775 1012 2940 Fragments arranged by size: 2940 2775 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3032 794 Size: 2238 4489 BstBI TT'CG_AA Cuts at: 5531 5531 Size: 6727 BstEII G'GTnAC_C Cuts at: 1184 2910 1184 Size: 1726 5001 BstYI r'GATC_y Cuts at: 1972 2409 2662 3867 4057 4424 4809 4826 6278 Size: 437 253 1205 190 367 385 17 1452 Cuts at: 6278 6364 6376 6722 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1205 437 385 367 346 253 190 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3461 3726 3461 Size: 265 6462 BtgI C'CryG_G Cuts at: 513 1065 3214 3316 3855 513 Size: 552 2149 102 539 3385 Fragments arranged by size: 3385 2149 552 539 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3024 487 Size: 683 56 1798 4190 Fragments arranged by size: 4190 1798 683 56 BtsI GCAGTG_nn' Cuts at: 2402 4243 5037 5057 2402 Size: 1841 794 20 4072 Fragments arranged by size: 4072 1841 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6727 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6692 DraI TTT'AAA Cuts at: 4031 4031 Size: 6727 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5734 809 Size: 507 4418 1802 Fragments arranged by size: 4418 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 2178 2520 Size: 54 1425 50 275 18 307 41 342 Cuts at: 2520 2568 2694 3238 3319 3451 3636 4174 5063 Size: 48 126 544 81 132 185 538 889 Cuts at: 5063 6692 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 544 538 342 307 275 185 132 126 81 54 50 48 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2568 3238 3451 3636 4174 1537 Size: 275 756 670 213 185 538 4090 Fragments arranged by size: 4090 756 670 538 275 213 185 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 3160 3903 3982 4657 1353 Size: 707 1100 743 79 675 3423 Fragments arranged by size: 3423 1100 743 707 675 79 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 3794 5314 5688 5834 387 Size: 1064 138 498 1707 1520 374 146 1280 Fragments arranged by size: 1707 1520 1280 1064 498 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 3157 3169 3181 4790 5387 6174 Size: 393 791 734 12 12 1609 597 787 Cuts at: 6174 6442 1239 Size: 268 1524 Fragments arranged by size: 1609 1524 791 787 734 597 393 268 12 12 EcoO109I rG'GnC_Cy Cuts at: 2110 3168 3396 3457 3996 2110 Size: 1058 228 61 539 4841 Fragments arranged by size: 4841 1058 539 228 61 EcoRI G'AATT_C Cuts at: 4036 4414 4036 Size: 378 6349 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3863 3863 Size: 6727 FseI GG_CCGG'CC Cuts at: 3638 3638 Size: 6727 FspI TGC'GCA Cuts at: 5233 5233 Size: 6727 HaeII r_GCGC'y Cuts at: 1678 2350 3123 3298 3761 3991 5874 1678 Size: 672 773 175 463 230 1883 2531 Fragments arranged by size: 2531 1883 773 672 463 230 175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2232 2264 2546 2578 5368 Size: 32 135 32 660 32 282 32 2790 Cuts at: 5368 5400 5442 5474 1373 Size: 32 42 32 2626 Fragments arranged by size: 2790 2626 660 282 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2056 4071 4317 669 Size: 988 281 118 2015 246 3079 Fragments arranged by size: 3079 2015 988 281 246 118 HindIII A'AGCT_T Cuts at: 748 2036 748 Size: 1288 5439 HpaI GTT'AAC Cuts at: 4317 4317 Size: 6727 KpnI G_GTAC'C Cuts at: 4052 4052 Size: 6727 MfeI C'AATT_G Cuts at: 4326 4326 Size: 6727 MluI A'CGCG_T Cuts at: 6603 6603 Size: 6727 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 5841 6025 1305 Size: 996 3540 184 2007 Fragments arranged by size: 3540 2007 996 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4905 Fragments arranged by size: 4905 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2618 3333 4685 5044 5203 518 Size: 648 1452 715 1352 359 159 2042 Fragments arranged by size: 2042 1452 1352 715 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2183 2567 2815 3032 3495 3686 4143 4820 Size: 577 384 248 217 463 191 457 677 Cuts at: 4820 5968 6213 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 577 463 457 384 248 245 217 191 NaeI GCC'GGC Cuts at: 2380 3323 3636 3932 2380 Size: 943 313 296 5175 Fragments arranged by size: 5175 943 313 296 NcoI C'CATG_G Cuts at: 513 1065 3855 513 Size: 552 2790 3385 Fragments arranged by size: 3385 2790 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6727 NgoMIV G'CCGG_C Cuts at: 2378 3321 3634 3930 2378 Size: 943 313 296 5175 Fragments arranged by size: 5175 943 313 296 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6727 NotI GC'GGCC_GC Cuts at: 4174 4174 Size: 6727 NspI r_CATG'y Cuts at: 1132 1229 2759 4028 4085 5630 1132 Size: 97 1530 1269 57 1545 2229 Fragments arranged by size: 2229 1545 1530 1269 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6727 PciI A'CATG_T Cuts at: 4024 5626 4024 Size: 1602 5125 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6727 PmeI GTTT'AAAC Cuts at: 4031 4031 Size: 6727 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4796 4828 6309 6341 4796 Size: 32 1481 32 5182 Fragments arranged by size: 5182 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2110 3168 3996 2110 Size: 1058 828 4841 Fragments arranged by size: 4841 1058 828 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6727 PsiI TTA'TAA Cuts at: 4297 4297 Size: 6727 PspOMI G'GGCC_C Cuts at: 3395 3395 Size: 6727 PspXI vC'TCGA_Gb Cuts at: 3163 3163 Size: 6727 PstI C_TGCA'G Cuts at: 830 2130 2170 4079 830 Size: 1300 40 1909 3478 Fragments arranged by size: 3478 1909 1300 40 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6727 PvuII CAG'CTG Cuts at: 3495 3495 Size: 6727 SalI G'TCGA_C Cuts at: 1936 2054 4069 1936 Size: 118 2015 4594 Fragments arranged by size: 4594 2015 118 SapI GCTCTTCn'nnn_ Cuts at: 3982 3982 Size: 6727 SbfI CC_TGCA'GG Cuts at: 2130 4079 2130 Size: 1949 4778 ScaI AGT'ACT Cuts at: 4975 6687 4975 Size: 1712 5015 SfcI C'TryA_G Cuts at: 826 1045 2126 2166 2350 3439 4075 5210 5891 Size: 219 1081 40 184 1089 636 1135 681 Cuts at: 5891 6082 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1089 1081 681 636 219 191 184 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6727 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6727 SmaI CCC'GGG Cuts at: 1872 2964 3105 4054 1872 Size: 1092 141 949 4545 Fragments arranged by size: 4545 1092 949 141 SmlI C'TyrA_G Cuts at: 820 1017 1943 2768 3163 3991 4831 5732 5994 Size: 197 926 825 395 828 840 901 262 Cuts at: 5994 820 Size: 1553 Fragments arranged by size: 1553 926 901 840 828 825 395 262 197 SnaBI TAC'GTA Cuts at: 493 4518 493 Size: 4025 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6727 SphI G_CATG'C Cuts at: 1132 2759 4085 1132 Size: 1627 1326 3774 Fragments arranged by size: 3774 1627 1326 SspI AAT'ATT Cuts at: 5 52 4651 5 Size: 47 4599 2081 Fragments arranged by size: 4599 2081 47 StuI AGG'CCT Cuts at: 1517 2358 2436 1517 Size: 841 78 5808 Fragments arranged by size: 5808 841 78 StyI C'CwwG_G Cuts at: 513 1065 2760 3855 4166 513 Size: 552 1695 1095 311 3074 Fragments arranged by size: 3074 1695 1095 552 311 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2253 2878 3689 4754 4771 4924 5109 Size: 1255 673 625 811 1065 17 153 185 Cuts at: 5109 5554 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1065 811 673 625 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2787 3924 4973 Size: 274 80 33 51 1443 810 1137 1049 Cuts at: 4973 6685 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1137 1049 810 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3236 3268 3347 3379 6687 6719 3236 Size: 32 79 32 3308 32 3244 Fragments arranged by size: 3308 3244 79 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2708 1316 Size: 1392 5335 XbaI T'CTAG_A Cuts at: 4063 4063 Size: 6727 XhoI C'TCGA_G Cuts at: 1943 3163 1943 Size: 1220 5507 XmaI C'CCGG_G Cuts at: 1870 2962 3103 4052 1870 Size: 1092 141 949 4545 Fragments arranged by size: 4545 1092 949 141 XmnI GAAnn'nnTTC Cuts at: 4856 4856 Size: 6727 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4535 276 Size: 53 83 186 936 3001 2468 Fragments arranged by size: 3001 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FspAI KasI NarI NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI