Restriction map for
pFN21AB4985
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspI AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FseI HaeIII
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I Van91I XhoI XhoII
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
AcyI 3 GGCGTC G[GA]^CG[CT]C
AfaI 1112 GTAC GT^AC
AflII 847 CTTAAG C^TTAAG
AflIII 1581 ACGTGT A^C[GA][CT]GT
AhdI 66 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 41 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 882 ATTAAT AT^TAAT
AsnI 882 ATTAAT AT^TAAT
AspEI 66 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 120 GAGCAC G[AT]GC[AT]^C
AvaI 761 CCCGGG C^[CT]CG[GA]G
AvaII 130 GGACC G^G[AT]CC
AviII 1191 TGCGCA TGC^GCA
BbsI 1066 GAAGAC GAAGAC(2/6)
BcgI 1037 GCAAACTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 732 GGATAC GTATCC(6/5)
BfrI 847 CTTAAG C^TTAAG
BglI 397 GCCACGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 586 CTGGAG CTGGAG(16/14)
BpuAI 1066 GAAGAC GAAGAC(2/6)
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BseRI 1226 GAGGAG GAGGAG(10/8)
BsiHKAI 120 GAGCAC G[AT]GC[AT]^C
BsoBI 761 CCCGGG C^[CT]CG[GA]G
BspHI 1055 TCATGA T^CATGA
BspMI 952 GCAGGT ACCTGC(4/8)
BstEII 186 GGTAACC G^GT[ACGT]ACC
BstF5I 364 CATCC GGATG(2/0)
BstPI 186 GGTAACC G^GT[ACGT]ACC
BstXI 134 CCAAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 130 GGACC G^G[ACGT]CC
DraII 129 GGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 66 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 52 GAAGAG CTCTTC(1/4)
EcoNI 1098 CCTTGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 129 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 186 GGTAACC G^GT[ACGT]ACC
FokI 364 CATCC GGATG(9/13)
FspI 1191 TGCGCA TGC^GCA
HaeII 417 GGCGCT [GA]GCGC^[CT]
HapII 762 CCGG C^CGG
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HindIII 1024 AAGCTT A^AGCTT
HpaI 25 GTTAAC GTT^AAC
HpaII 762 CCGG C^CGG
Ksp632I 52 GAAGAG CTCTTC(1/4)
MspA1I 1520 CAGCTG C[AC]G^C[GT]G
MspI 762 CCGG C^CGG
PpuMI 129 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 882 ATTAAT AT^TAAT
PstI 431 CTGCAG CTGCA^G
PvuII 1520 CAGCTG CAG^CTG
RcaI 1055 TCATGA T^CATGA
RsaI 1112 GTAC GT^AC
Sau96I 130 GGACC G^G[ACGT]CC
SexAI 1379 ACCTGGT A^CC[AT]GGT
SmaI 761 CCCGGG CCC^GGG
SmlI 847 CTTAAG C^T[CT][GA]AG
SphI 939 GCATGC GCATG^C
SspI 481 AATATT AAT^ATT
TaqI 1046 TCGA T^CGA
TthHB8I 1046 TCGA T^CGA
XbaI 1210 TCTAGA T^CTAGA
XmaCI 761 CCCGGG C^CCGGG
XmaI 761 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BcnI 761 CCCGG CC^[GC]GG
762 CCGGG
BmyI 120 GAGCAC G[AGT]GC[ACT]^C
489 GGGCAC
BsgI 1373 GTGCAG GTGCAG(16/14)
1517 GTGCAG
BsiYI 1011 CCCAGAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1098 CCTTGGTGAGG
BslI 1011 CCCAGAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1098 CCTTGGTGAGG
BsmAI 64 GAGAC GTCTC(1/5)
107 GAGAC
Bsp1286I 120 GAGCAC G[AGT]GC[ACT]^C
489 GGGCAC
BsrDI 1166 CATTGC GCAATG(2/0)
1303 GCAATG
BsrI 724 ACTGG ACTGG(1/-1)
1017 ACTGG
BstAPI 784 GCAAAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
943 GCAGATTTTGC
CfoI 418 GCGC GCG^C
1192 GCGC
EcoT14I 125 CCTTGG C^C[AT][AT]GG
1098 CCTTGG
FauI 382 GCGGG CCCGC(4/6)
487 GCGGG
HhaI 418 GCGC GCG^C
1192 GCGC
HinP1I 418 GCGC G^CGC
1192 GCGC
MaeII 840 ACGT A^CGT
1581 ACGT
NciI 761 CCCGG CC^[GC]GG
762 CCGGG
NspI 804 ACATGC [GA]CATG^[CT]
939 GCATGC
SfaNI 172 GATGC GCATC(5/9)
1174 GATGC
SfcI 431 CTGCAG C^T[GA][CT]AG
548 CTGTAG
StyI 125 CCTTGG C^C[AT][AT]GG
1098 CCTTGG
Tsp45I 976 GTCAC ^GT[GC]AC
1261 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
BanI BbvI BclI BfaI BsaJI
BsmFI BssKI BstNI Cac8I DdeI
DpnII EcoRII FbaI Fnu4HI HincII
HindII HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI ScrFI TfiI Tru9I
TseI Tsp509I TspRI XcmI XmnI