(Circular) (Six-base) MAPSORT of: pFN21AB4985.seq Check: 7731 from: 1 to: 6310 pFN21AB4985 6310 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2948 2948 Size: 6310 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4120 278 Size: 53 83 186 936 2584 2468 Fragments arranged by size: 2584 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3653 1937 Size: 1716 4594 Acc65I G'GTAC_C Cuts at: 3631 3631 Size: 6310 AclI AA'CG_TT Cuts at: 1563 4437 4810 1563 Size: 2874 373 3063 Fragments arranged by size: 3063 2874 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3549 4373 5757 6025 1632 Size: 1917 824 1384 268 1917 Fragments arranged by size: 1917 1917 1384 824 268 AflII C'TTAA_G Cuts at: 820 1017 2852 820 Size: 197 1835 4278 Fragments arranged by size: 4278 1835 197 AflIII A'CryG_T Cuts at: 1203 3586 5209 6186 1203 Size: 2383 1623 977 1327 Fragments arranged by size: 2383 1623 1327 977 AgeI A'CCGG_T Cuts at: 5141 5141 Size: 6310 AhdI GACnn_n'nnGTC Cuts at: 2076 5039 2076 Size: 2963 3347 AleI CACnn'nnGTG Cuts at: 1166 3606 1166 Size: 2440 3870 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6310 AlwNI CAG_nnn'CTG Cuts at: 2051 4053 5625 2051 Size: 2002 1572 2736 Fragments arranged by size: 2736 2002 1572 ApaLI G'TGCA_C Cuts at: 4367 5523 4367 Size: 1156 5154 ApoI r'AATT_y Cuts at: 1493 2154 2465 2712 3147 3619 3845 3997 6106 Size: 661 311 247 435 472 226 152 2109 Cuts at: 6106 1493 Size: 1697 Fragments arranged by size: 2109 1697 661 472 435 311 247 226 152 AseI AT'TA_AT Cuts at: 160 1032 2888 4864 160 Size: 872 1856 1976 1606 Fragments arranged by size: 1976 1856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6310 AvaI C'yCGr_G Cuts at: 1870 1943 2766 3635 1870 Size: 73 823 869 4545 Fragments arranged by size: 4545 869 823 73 BamHI G'GATC_C Cuts at: 3640 4007 3640 Size: 367 5943 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2096 2204 3134 3452 3574 Size: 325 217 627 309 108 930 318 122 Cuts at: 3574 3631 5086 618 Size: 57 1455 1842 Fragments arranged by size: 1842 1455 930 627 325 318 309 217 122 108 57 BanII G_rGCy'C Cuts at: 1391 3629 1391 Size: 2238 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3078 928 Size: 971 1179 4160 Fragments arranged by size: 4160 1179 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3031 3065 4501 4535 1903 Size: 34 1094 34 1436 34 3678 Fragments arranged by size: 3678 1436 1094 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2731 4202 5412 2731 Size: 1471 1210 3629 Fragments arranged by size: 3629 1471 1210 BclI T'GATC_A Cuts at: 1548 2443 3010 3595 1548 Size: 895 567 585 4263 Fragments arranged by size: 4263 895 585 567 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2408 4921 136 Size: 107 122 71 631 605 736 2513 1525 Fragments arranged by size: 2513 1525 736 631 605 122 107 71 BglII A'GATC_T Cuts at: 6305 6305 Size: 6310 BlpI GC'TnA_GC Cuts at: 3727 3727 Size: 6310 Bme1580I G_kGCm'C Cuts at: 1790 2498 4371 5527 1790 Size: 708 1873 1156 2573 Fragments arranged by size: 2573 1873 1156 708 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6310 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4041 4999 442 Size: 416 53 83 399 24 2624 958 1753 Fragments arranged by size: 2624 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6310 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2612 4970 1239 Size: 1373 2358 2579 Fragments arranged by size: 2579 2358 1373 Bpu10I CC'TnA_GC Cuts at: 3398 3398 Size: 6310 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4435 5300 5598 4435 Size: 865 298 5147 Fragments arranged by size: 5147 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4973 882 Size: 617 3474 2219 Fragments arranged by size: 3474 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4101 493 Size: 711 2897 2702 Fragments arranged by size: 2897 2702 711 BsaBI GATnn'nnATC Cuts at: 6304 6304 Size: 6310 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 4117 4499 5134 Size: 53 83 186 936 476 2108 382 635 Cuts at: 5134 275 Size: 1451 Fragments arranged by size: 2108 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4743 5141 5415 5562 5973 4743 Size: 398 274 147 411 5080 Fragments arranged by size: 5080 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2103 2133 1178 Size: 30 165 30 700 30 5355 Fragments arranged by size: 5355 700 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3246 1186 Size: 2060 4250 BseYI C'CCAG_C Cuts at: 5513 6009 5513 Size: 496 5814 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3399 3543 1163 Size: 573 1663 144 3930 Fragments arranged by size: 3930 1663 573 144 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3760 4521 5549 1540 Size: 275 186 1759 761 1028 2301 Fragments arranged by size: 2301 1759 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2129 4371 4456 5527 2129 Size: 2242 85 1071 2912 Fragments arranged by size: 2912 2242 1071 85 BsmI GAATG_Cn' Cuts at: 3821 3914 3821 Size: 93 6217 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2498 3629 4371 4456 5527 1391 Size: 399 339 369 1131 742 85 1071 2174 Fragments arranged by size: 2174 1131 1071 742 399 369 339 85 BspEI T'CCGG_A Cuts at: 5973 5973 Size: 6310 BspHI T'CATG_A Cuts at: 3060 4199 5929 3060 Size: 1139 1730 3441 Fragments arranged by size: 3441 1730 1139 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2948 3665 844 Size: 2104 717 3489 Fragments arranged by size: 3489 2104 717 BsrBI CCG'CTC Cuts at: 1394 1823 4197 1394 Size: 429 2374 3507 Fragments arranged by size: 3507 2374 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3170 3315 4805 4979 65 Size: 1183 1922 145 1490 174 1396 Fragments arranged by size: 1922 1490 1396 1183 174 145 BsrFI r'CCGG_y Cuts at: 1700 1810 4954 5141 1700 Size: 110 3144 187 2869 Fragments arranged by size: 3144 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6310 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6310 BssSI C'ACGA_G Cuts at: 4370 5382 4370 Size: 1012 5298 BstAPI GCAn_nnn'nTGC Cuts at: 794 2795 2954 794 Size: 2001 159 4150 Fragments arranged by size: 4150 2001 159 BstBI TT'CG_AA Cuts at: 5114 5114 Size: 6310 BstEII G'GTnAC_C Cuts at: 1184 2191 1184 Size: 1007 5303 BstXI CCAn_nnnn'nTGG Cuts at: 2146 2146 Size: 6310 BstYI r'GATC_y Cuts at: 1972 3640 4007 4392 4409 5861 5947 5959 6305 Size: 1668 367 385 17 1452 86 12 346 Cuts at: 6305 1972 Size: 1977 Fragments arranged by size: 1977 1668 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4819 Fragments arranged by size: 4819 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2086 487 Size: 683 56 860 4711 Fragments arranged by size: 4711 860 683 56 BtsI GCAGTG_nn' Cuts at: 2173 3426 3826 4620 4640 2173 Size: 1253 400 794 20 3843 Fragments arranged by size: 3843 1253 794 400 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6310 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6275 DraI TTT'AAA Cuts at: 3614 3614 Size: 6310 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5317 809 Size: 507 4001 1802 Fragments arranged by size: 4001 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3757 4646 6275 Size: 54 1425 50 275 18 1927 889 1629 Cuts at: 6275 8 Size: 43 Fragments arranged by size: 1927 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3757 1537 Size: 275 1945 4090 Fragments arranged by size: 4090 1945 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 4240 1353 Size: 699 2188 3423 Fragments arranged by size: 3423 2188 699 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4897 5271 5417 387 Size: 1064 138 3308 374 146 1280 Fragments arranged by size: 3308 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2612 3549 4373 4970 5757 6025 1239 Size: 393 980 937 824 597 787 268 1524 Fragments arranged by size: 1524 980 937 824 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3107 3107 Size: 6310 EcoO109I rG'GnC_Cy Cuts at: 2135 2135 Size: 6310 EcoRI G'AATT_C Cuts at: 3619 3997 3619 Size: 378 5932 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2146 2146 Size: 6310 FspI TGC'GCA Cuts at: 3198 4816 3198 Size: 1618 4692 HaeII r_GCGC'y Cuts at: 1678 2426 5457 1678 Size: 748 3031 2531 Fragments arranged by size: 3031 2531 748 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2062 2094 4951 4983 5025 Size: 32 135 32 490 32 2857 32 42 Cuts at: 5025 5057 1373 Size: 32 2626 Fragments arranged by size: 2857 2626 490 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2032 2233 2779 3654 3900 669 Size: 988 281 94 201 546 875 246 3079 Fragments arranged by size: 3079 988 875 546 281 246 201 94 HindIII A'AGCT_T Cuts at: 748 3029 748 Size: 2281 4029 HpaI GTT'AAC Cuts at: 2032 3900 2032 Size: 1868 4442 KpnI G_GTAC'C Cuts at: 3635 3635 Size: 6310 MfeI C'AATT_G Cuts at: 3909 3909 Size: 6310 MluI A'CGCG_T Cuts at: 6186 6186 Size: 6310 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2372 2643 5424 5608 1305 Size: 1067 271 2781 184 2007 Fragments arranged by size: 2781 2007 1067 271 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4488 Fragments arranged by size: 4488 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3606 4268 4627 4786 518 Size: 648 2440 662 359 159 2042 Fragments arranged by size: 2440 2042 662 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3527 3726 4403 5551 5796 1606 Size: 1921 199 677 1148 245 2120 Fragments arranged by size: 2120 1921 1148 677 245 199 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4819 Fragments arranged by size: 4819 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6310 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6310 NotI GC'GGCC_GC Cuts at: 3757 3757 Size: 6310 NspI r_CATG'y Cuts at: 1132 1229 2813 2948 3668 5213 1132 Size: 97 1584 135 720 1545 2229 Fragments arranged by size: 2229 1584 1545 720 135 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6310 PciI A'CATG_T Cuts at: 5209 5209 Size: 6310 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6310 PmeI GTTT'AAAC Cuts at: 3614 3614 Size: 6310 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4379 4411 5892 5924 4379 Size: 32 1481 32 4765 Fragments arranged by size: 4765 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2135 2135 Size: 6310 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6310 PsiI TTA'TAA Cuts at: 2895 3880 2895 Size: 985 5325 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2547 2579 2547 Size: 32 6278 PstI C_TGCA'G Cuts at: 830 2440 3662 830 Size: 1610 1222 3478 Fragments arranged by size: 3478 1610 1222 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6310 PvuII CAG'CTG Cuts at: 3527 3527 Size: 6310 SalI G'TCGA_C Cuts at: 1936 3652 1936 Size: 1716 4594 SanDI GG'GwC_CC Cuts at: 2135 2135 Size: 6310 SbfI CC_TGCA'GG Cuts at: 3662 3662 Size: 6310 ScaI AGT'ACT Cuts at: 4558 6270 4558 Size: 1712 4598 SexAI A'CCwGG_T Cuts at: 3384 3384 Size: 6310 SfcI C'TryA_G Cuts at: 826 1045 2436 2553 3658 4793 5474 5665 826 Size: 219 1391 117 1105 1135 681 191 1471 Fragments arranged by size: 1471 1391 1135 1105 681 219 191 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6310 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6310 SmaI CCC'GGG Cuts at: 1872 2768 3637 1872 Size: 896 869 4545 Fragments arranged by size: 4545 896 869 SmlI C'TyrA_G Cuts at: 820 1017 1943 2852 4414 5315 5577 820 Size: 197 926 909 1562 901 262 1553 Fragments arranged by size: 1562 1553 926 909 901 262 197 SnaBI TAC'GTA Cuts at: 493 4101 493 Size: 3608 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6310 SphI G_CATG'C Cuts at: 1132 2948 3668 1132 Size: 1816 720 3774 Fragments arranged by size: 3774 1816 720 SspI AAT'ATT Cuts at: 5 52 2488 4234 5 Size: 47 2436 1746 2081 Fragments arranged by size: 2436 2081 1746 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6310 StyI C'CwwG_G Cuts at: 513 1065 2004 2130 3103 3749 513 Size: 552 939 126 973 646 3074 Fragments arranged by size: 3074 973 939 646 552 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3030 3592 4337 4354 4507 4692 5137 Size: 1255 1450 562 745 17 153 185 445 Cuts at: 5137 325 Size: 1498 Fragments arranged by size: 1498 1450 1255 745 562 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4556 6268 96 Size: 274 80 33 51 1443 2579 1712 138 Fragments arranged by size: 2579 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6270 6302 6270 Size: 32 6278 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6310 XbaI T'CTAG_A Cuts at: 3215 3646 3215 Size: 431 5879 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2113 3433 3463 2113 Size: 1320 30 4960 Fragments arranged by size: 4960 1320 30 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6310 XmaI C'CCGG_G Cuts at: 1870 2766 3635 1870 Size: 896 869 4545 Fragments arranged by size: 4545 896 869 XmnI GAAnn'nnTTC Cuts at: 2562 2847 3492 4439 2562 Size: 285 645 947 4433 Fragments arranged by size: 4433 947 645 285 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4118 276 Size: 53 83 186 936 2584 2468 Fragments arranged by size: 2584 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI