(Circular) (Six-base) MAPSORT of: pFN21AB4982.seq Check: 5982 from: 1 to: 7108 pFN21AB4982 7108 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4918 278 Size: 53 83 186 936 3382 2468 Fragments arranged by size: 3382 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4451 1937 Size: 2514 4594 Acc65I G'GTAC_C Cuts at: 4429 4429 Size: 7108 AclI AA'CG_TT Cuts at: 1563 2592 5235 5608 1563 Size: 1029 2643 373 3063 Fragments arranged by size: 3063 2643 1029 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2421 3027 5171 6555 6823 1632 Size: 789 606 2144 1384 268 1917 Fragments arranged by size: 2144 1917 1384 789 606 268 AflII C'TTAA_G Cuts at: 820 1017 4025 820 Size: 197 3008 3903 Fragments arranged by size: 3903 3008 197 AflIII A'CryG_T Cuts at: 1203 6007 6984 1203 Size: 4804 977 1327 Fragments arranged by size: 4804 1327 977 AgeI A'CCGG_T Cuts at: 5939 5939 Size: 7108 AhdI GACnn_n'nnGTC Cuts at: 5837 5837 Size: 7108 AleI CACnn'nnGTG Cuts at: 1166 3411 3861 1166 Size: 2245 450 4413 Fragments arranged by size: 4413 2245 450 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2099 1184 Size: 915 6193 AlwNI CAG_nnn'CTG Cuts at: 2747 4337 4851 6423 2747 Size: 1590 514 1572 3432 Fragments arranged by size: 3432 1590 1572 514 ApaI G_GGCC'C Cuts at: 4269 4269 Size: 7108 ApaLI G'TGCA_C Cuts at: 5165 6321 5165 Size: 1156 5952 ApoI r'AATT_y Cuts at: 1493 3422 3502 4417 4643 4795 6904 1493 Size: 1929 80 915 226 152 2109 1697 Fragments arranged by size: 2109 1929 1697 915 226 152 80 AseI AT'TA_AT Cuts at: 160 1032 5662 160 Size: 872 4630 1606 Fragments arranged by size: 4630 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7108 AvaI C'yCGr_G Cuts at: 1870 1943 4433 1870 Size: 73 2490 4545 Fragments arranged by size: 4545 2490 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3931 3964 3931 Size: 33 7075 BamHI G'GATC_C Cuts at: 4438 4805 4438 Size: 367 6741 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2525 4429 5884 618 Size: 325 217 627 738 1904 1455 1842 Fragments arranged by size: 1904 1842 1455 738 627 325 217 BanII G_rGCy'C Cuts at: 1391 2192 2796 4269 4427 1391 Size: 801 604 1473 158 4072 Fragments arranged by size: 4072 1473 801 604 158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2528 3090 4356 928 Size: 971 629 562 1266 3680 Fragments arranged by size: 3680 1266 971 629 562 BbvCI CC'TCA_GC Cuts at: 2969 2969 Size: 7108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5299 5333 1903 Size: 34 50 34 3278 34 3678 Fragments arranged by size: 3678 3278 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5000 6210 5000 Size: 1210 5898 BclI T'GATC_A Cuts at: 1548 1548 Size: 7108 BfrBI ATG'CAT Cuts at: 2008 2776 2008 Size: 768 6340 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5719 136 Size: 107 122 71 631 605 4047 1525 Fragments arranged by size: 4047 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4324 7103 4324 Size: 2779 4329 BlpI GC'TnA_GC Cuts at: 4525 4525 Size: 7108 Bme1580I G_kGCm'C Cuts at: 1790 2530 2857 3791 4269 5169 6325 1790 Size: 740 327 934 478 900 1156 2573 Fragments arranged by size: 2573 1156 934 900 740 478 327 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7108 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2150 2538 4020 Size: 416 53 83 399 24 733 388 1482 Cuts at: 4020 4839 5797 442 Size: 819 958 1753 Fragments arranged by size: 1753 1482 958 819 733 416 399 388 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7108 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4327 4327 Size: 7108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 2674 2810 3312 4007 4258 5768 1239 Size: 1085 350 136 502 695 251 1510 2579 Fragments arranged by size: 2579 1510 1085 695 502 350 251 136 Bpu10I CC'TnA_GC Cuts at: 2186 2969 2186 Size: 783 6325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3899 4055 4080 4159 5233 6098 6396 3899 Size: 156 25 79 1074 865 298 4611 Fragments arranged by size: 4611 1074 865 298 156 79 25 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5771 882 Size: 617 4272 2219 Fragments arranged by size: 4272 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4081 4899 493 Size: 711 2877 818 2702 Fragments arranged by size: 2877 2702 818 711 BsaBI GATnn'nnATC Cuts at: 2451 3636 7102 2451 Size: 1185 3466 2457 Fragments arranged by size: 3466 2457 1185 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4915 5297 5932 275 Size: 53 83 186 936 3382 382 635 1451 Fragments arranged by size: 3382 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5541 5939 6213 6360 6771 5541 Size: 398 274 147 411 5878 Fragments arranged by size: 5878 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2266 2296 2918 2948 3261 Size: 30 165 30 863 30 622 30 313 Cuts at: 3261 3291 1178 Size: 30 4995 Fragments arranged by size: 4995 863 622 313 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2651 2983 4307 1186 Size: 1465 332 1324 3987 Fragments arranged by size: 3987 1465 1324 332 BseYI C'CCAG_C Cuts at: 3893 6311 6807 3893 Size: 2418 496 4194 Fragments arranged by size: 4194 2418 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3526 4383 1163 Size: 573 1790 857 3888 Fragments arranged by size: 3888 1790 857 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4558 5319 6347 1540 Size: 275 186 2557 761 1028 2301 Fragments arranged by size: 2557 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2192 2796 2891 3782 4086 5169 5254 6325 2192 Size: 604 95 891 304 1083 85 1071 2975 Fragments arranged by size: 2975 1083 1071 891 604 304 95 85 BsmI GAATG_Cn' Cuts at: 2668 4619 4712 2668 Size: 1951 93 5064 Fragments arranged by size: 5064 1951 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2192 2530 2796 2857 2891 3782 3791 Size: 399 402 338 266 61 34 891 9 Cuts at: 3791 4086 4269 4427 5169 5254 6325 1391 Size: 295 183 158 742 85 1071 2174 Fragments arranged by size: 2174 1071 891 742 402 399 338 295 266 183 158 85 61 34 9 BspEI T'CCGG_A Cuts at: 6771 6771 Size: 7108 BspHI T'CATG_A Cuts at: 2698 3520 4174 4997 6727 2698 Size: 822 654 823 1730 3079 Fragments arranged by size: 3079 1730 823 822 654 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3909 4265 4463 844 Size: 3065 356 198 3489 Fragments arranged by size: 3489 3065 356 198 BsrBI CCG'CTC Cuts at: 1394 1823 4995 1394 Size: 429 3172 3507 Fragments arranged by size: 3507 3172 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2083 2820 5603 5777 65 Size: 1183 835 737 2783 174 1396 Fragments arranged by size: 2783 1396 1183 835 737 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5752 5939 1700 Size: 110 3942 187 2869 Fragments arranged by size: 3942 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4208 96 Size: 4112 2996 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7108 BssSI C'ACGA_G Cuts at: 5168 6180 5168 Size: 1012 6096 BstAPI GCAn_nnn'nTGC Cuts at: 794 3660 4081 794 Size: 2866 421 3821 Fragments arranged by size: 3821 2866 421 BstBI TT'CG_AA Cuts at: 5912 5912 Size: 7108 BstEII G'GTnAC_C Cuts at: 1184 2256 2602 1184 Size: 1072 346 5690 Fragments arranged by size: 5690 1072 346 BstXI CCAn_nnnn'nTGG Cuts at: 2963 2963 Size: 7108 BstYI r'GATC_y Cuts at: 1972 2446 4324 4438 4805 5190 5207 6659 6745 Size: 474 1878 114 367 385 17 1452 86 Cuts at: 6745 6757 7103 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1878 1452 474 385 367 346 114 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3351 3742 513 Size: 552 2286 391 3879 Fragments arranged by size: 3879 2286 552 391 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6369 Fragments arranged by size: 6369 683 56 BtsI GCAGTG_nn' Cuts at: 3071 4624 5418 5438 3071 Size: 1553 794 20 4741 Fragments arranged by size: 4741 1553 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7108 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7073 DraI TTT'AAA Cuts at: 4412 4412 Size: 7108 DraIII CAC_nnn'GTG Cuts at: 2525 2878 2525 Size: 353 6755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6115 809 Size: 507 4799 1802 Fragments arranged by size: 4799 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2551 2880 3334 Size: 54 1425 50 275 18 721 329 454 Cuts at: 3334 4555 5444 7073 8 Size: 1221 889 1629 43 Fragments arranged by size: 1629 1425 1221 889 721 454 329 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4555 1537 Size: 275 2743 4090 Fragments arranged by size: 4090 2743 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3489 4296 4311 5038 1353 Size: 2136 807 15 727 3423 Fragments arranged by size: 3423 2136 807 727 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5695 6069 6215 387 Size: 1064 138 4106 374 146 1280 Fragments arranged by size: 4106 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2190 2794 2190 Size: 604 6504 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2421 2674 2810 3027 3312 4007 Size: 393 692 97 253 136 217 285 695 Cuts at: 4007 4258 5171 5768 6555 6823 1239 Size: 251 913 597 787 268 1524 Fragments arranged by size: 1524 913 787 695 692 597 393 285 268 253 251 217 136 97 EcoNI CCTnn'n_nnAGG Cuts at: 2145 2145 Size: 7108 EcoO109I rG'GnC_Cy Cuts at: 2177 4066 4265 2177 Size: 1889 199 5020 Fragments arranged by size: 5020 1889 199 EcoRI G'AATT_C Cuts at: 3422 4417 4795 3422 Size: 995 378 5735 Fragments arranged by size: 5735 995 378 EcoRV GAT'ATC Cuts at: 3556 3556 Size: 7108 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4153 4153 Size: 7108 FspI TGC'GCA Cuts at: 3476 5614 3476 Size: 2138 4970 HaeII r_GCGC'y Cuts at: 1678 6255 1678 Size: 4577 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2492 2524 4099 4131 4132 Size: 32 135 32 920 32 1575 32 1 Cuts at: 4132 4164 5749 5781 5823 5855 1373 Size: 32 1585 32 42 32 2626 Fragments arranged by size: 2626 1585 1575 920 135 42 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 3385 4452 4698 669 Size: 988 281 1447 1067 246 3079 Fragments arranged by size: 3079 1447 1067 988 281 246 HindIII A'AGCT_T Cuts at: 748 3795 748 Size: 3047 4061 HpaI GTT'AAC Cuts at: 3385 4698 3385 Size: 1313 5795 KpnI G_GTAC'C Cuts at: 4433 4433 Size: 7108 MfeI C'AATT_G Cuts at: 3261 4707 3261 Size: 1446 5662 MluI A'CGCG_T Cuts at: 6984 6984 Size: 7108 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2452 6222 6406 1305 Size: 1147 3770 184 2007 Fragments arranged by size: 3770 2007 1147 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2553 2882 3336 10 Size: 54 1425 343 721 329 454 3782 Fragments arranged by size: 3782 1425 721 454 343 329 54 MslI CAynn'nnrTG Cuts at: 518 1166 2544 2760 2781 3033 3305 3411 3630 Size: 648 1378 216 21 252 272 106 219 Cuts at: 3630 3845 3861 5066 5425 5584 518 Size: 215 16 1205 359 159 2042 Fragments arranged by size: 2042 1378 1205 648 359 272 252 219 216 215 159 106 21 16 MspA1I CmG'CkG Cuts at: 1606 2056 4382 4524 5201 6349 6594 1606 Size: 450 2326 142 677 1148 245 2120 Fragments arranged by size: 2326 2120 1148 677 450 245 142 NcoI C'CATG_G Cuts at: 513 1065 3351 3742 513 Size: 552 2286 391 3879 Fragments arranged by size: 3879 2286 552 391 NdeI CA'TA_TG Cuts at: 387 387 Size: 7108 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7108 NotI GC'GGCC_GC Cuts at: 4555 4555 Size: 7108 NsiI A_TGCA'T Cuts at: 2010 2778 2010 Size: 768 6340 NspI r_CATG'y Cuts at: 1132 1229 2845 4466 6011 1132 Size: 97 1616 1621 1545 2229 Fragments arranged by size: 2229 1621 1616 1545 97 PasI CC'CwG_GG Cuts at: 1348 3724 1348 Size: 2376 4732 PciI A'CATG_T Cuts at: 6007 6007 Size: 7108 PflMI CCAn_nnn'nTGG Cuts at: 1108 2560 3832 1108 Size: 1452 1272 4384 Fragments arranged by size: 4384 1452 1272 PmeI GTTT'AAAC Cuts at: 4412 4412 Size: 7108 PmlI CAC'GTG Cuts at: 4081 4081 Size: 7108 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5177 5209 6690 6722 5177 Size: 32 1481 32 5563 Fragments arranged by size: 5563 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2177 4066 2177 Size: 1889 5219 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7108 PsiI TTA'TAA Cuts at: 4678 4678 Size: 7108 PspOMI G'GGCC_C Cuts at: 4265 4265 Size: 7108 PstI C_TGCA'G Cuts at: 830 4460 830 Size: 3630 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7108 PvuII CAG'CTG Cuts at: 2056 2056 Size: 7108 SacI G_AGCT'C Cuts at: 2192 2796 2192 Size: 604 6504 SalI G'TCGA_C Cuts at: 1936 4450 1936 Size: 2514 4594 SanDI GG'GwC_CC Cuts at: 2177 2177 Size: 7108 SapI GCTCTTCn'nnn_ Cuts at: 3489 3489 Size: 7108 SbfI CC_TGCA'GG Cuts at: 4460 4460 Size: 7108 ScaI AGT'ACT Cuts at: 5356 7068 5356 Size: 1712 5396 SfcI C'TryA_G Cuts at: 826 1045 2748 3871 3940 4354 4456 5591 6272 Size: 219 1703 1123 69 414 102 1135 681 Cuts at: 6272 6463 826 Size: 191 1471 Fragments arranged by size: 1703 1471 1135 1123 681 414 219 191 102 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7108 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7108 SmaI CCC'GGG Cuts at: 1872 4435 1872 Size: 2563 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3914 4025 4070 4095 4138 5212 Size: 197 926 1971 111 45 25 43 1074 Cuts at: 5212 6113 6375 820 Size: 901 262 1553 Fragments arranged by size: 1971 1553 1074 926 901 262 197 111 45 43 25 SnaBI TAC'GTA Cuts at: 493 4899 493 Size: 4406 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7108 SphI G_CATG'C Cuts at: 1132 4466 1132 Size: 3334 3774 SspI AAT'ATT Cuts at: 5 52 5032 5 Size: 47 4980 2081 Fragments arranged by size: 4980 2081 47 StuI AGG'CCT Cuts at: 1517 2151 1517 Size: 634 6474 StyI C'CwwG_G Cuts at: 513 1065 2565 3351 3742 4547 513 Size: 552 1500 786 391 805 3074 Fragments arranged by size: 3074 1500 805 786 552 391 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2137 3017 3642 3986 5135 5152 5305 Size: 1255 557 880 625 344 1149 17 153 Cuts at: 5305 5490 5935 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1149 880 625 557 445 344 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2373 3134 4208 Size: 274 80 33 51 1443 396 761 1074 Cuts at: 4208 5354 7066 96 Size: 1146 1712 138 Fragments arranged by size: 1712 1443 1146 1074 761 396 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2266 2298 2361 2393 3540 3572 3974 4006 4351 Size: 32 63 32 1147 32 402 32 345 Cuts at: 4351 4383 7068 7100 2266 Size: 32 2685 32 2274 Fragments arranged by size: 2685 2274 1147 402 345 63 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7108 XbaI T'CTAG_A Cuts at: 4444 4444 Size: 7108 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2681 2875 2681 Size: 194 6914 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7108 XmaI C'CCGG_G Cuts at: 1870 4433 1870 Size: 2563 4545 XmnI GAAnn'nnTTC Cuts at: 3300 5237 3300 Size: 1937 5171 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4916 276 Size: 53 83 186 936 3382 2468 Fragments arranged by size: 3382 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BsiWI BsmBI BstZ17I Bsu36I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI