(Circular) (Six-base) MAPSORT of: pFN21AB4944.seq Check: 3512 from: 1 to: 6241 pFN21AB4944 6241 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4051 278 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3584 1937 Size: 1647 4594 Acc65I G'GTAC_C Cuts at: 3562 3562 Size: 6241 AclI AA'CG_TT Cuts at: 1563 4368 4741 1563 Size: 2805 373 3063 Fragments arranged by size: 3063 2805 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3359 4304 5688 5956 1632 Size: 1727 945 1384 268 1917 Fragments arranged by size: 1917 1727 1384 945 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6044 AflIII A'CryG_T Cuts at: 1203 5140 6117 1203 Size: 3937 977 1327 Fragments arranged by size: 3937 1327 977 AgeI A'CCGG_T Cuts at: 5072 5072 Size: 6241 AhdI GACnn_n'nnGTC Cuts at: 4970 4970 Size: 6241 AleI CACnn'nnGTG Cuts at: 1166 3462 1166 Size: 2296 3945 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6241 AlwNI CAG_nnn'CTG Cuts at: 3984 5556 3984 Size: 1572 4669 ApaLI G'TGCA_C Cuts at: 4298 5454 4298 Size: 1156 5085 ApoI r'AATT_y Cuts at: 1493 2792 3550 3776 3928 6037 1493 Size: 1299 758 226 152 2109 1697 Fragments arranged by size: 2109 1697 1299 758 226 152 AseI AT'TA_AT Cuts at: 160 1032 2406 3423 4795 160 Size: 872 1374 1017 1372 1606 Fragments arranged by size: 1606 1374 1372 1017 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 AvaI C'yCGr_G Cuts at: 1870 1943 3348 3566 1870 Size: 73 1405 218 4545 Fragments arranged by size: 4545 1405 218 73 BamHI G'GATC_C Cuts at: 3571 3938 3571 Size: 367 5874 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3562 5017 618 Size: 325 217 627 1775 1455 1842 Fragments arranged by size: 1842 1775 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2833 3560 1391 Size: 1442 727 4072 Fragments arranged by size: 4072 1442 727 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2225 2537 2726 928 Size: 971 326 312 189 4443 Fragments arranged by size: 4443 971 326 312 189 BbvCI CC'TCA_GC Cuts at: 3487 3487 Size: 6241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4432 4466 1903 Size: 34 2495 34 3678 Fragments arranged by size: 3678 2495 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4133 5343 4133 Size: 1210 5031 BclI T'GATC_A Cuts at: 1548 1548 Size: 6241 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4852 136 Size: 107 122 71 631 605 3180 1525 Fragments arranged by size: 3180 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6236 6236 Size: 6241 BlpI GC'TnA_GC Cuts at: 3658 3658 Size: 6241 Bme1580I G_kGCm'C Cuts at: 1790 4302 5458 1790 Size: 2512 1156 2573 Fragments arranged by size: 2573 2512 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6241 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3972 4930 442 Size: 416 53 83 399 24 2555 958 1753 Fragments arranged by size: 2555 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4901 1239 Size: 3662 2579 Bpu10I CC'TnA_GC Cuts at: 3487 3487 Size: 6241 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3239 4366 5231 5529 3239 Size: 1127 865 298 3951 Fragments arranged by size: 3951 1127 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4904 882 Size: 617 3405 2219 Fragments arranged by size: 3405 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4032 493 Size: 711 2828 2702 Fragments arranged by size: 2828 2702 711 BsaBI GATnn'nnATC Cuts at: 6235 6235 Size: 6241 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3291 4048 4430 5065 Size: 53 83 186 936 1758 757 382 635 Cuts at: 5065 275 Size: 1451 Fragments arranged by size: 1758 1451 936 757 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3387 4674 5072 5346 5493 5904 3387 Size: 1287 398 274 147 411 3724 Fragments arranged by size: 3724 1287 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2638 2668 1178 Size: 30 165 30 1235 30 4751 Fragments arranged by size: 4751 1235 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6241 BseYI C'CCAG_C Cuts at: 2849 5444 5940 2849 Size: 2595 496 3150 Fragments arranged by size: 3150 2595 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5668 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2041 3691 4452 5480 1540 Size: 275 186 40 1650 761 1028 2301 Fragments arranged by size: 2301 1650 1028 761 275 186 40 BsiHKAI G_wGCw'C Cuts at: 2733 2762 3099 4302 4387 5458 2733 Size: 29 337 1203 85 1071 3516 Fragments arranged by size: 3516 1203 1071 337 85 29 BsmI GAATG_Cn' Cuts at: 2453 3438 3752 3845 2453 Size: 985 314 93 4849 Fragments arranged by size: 4849 985 314 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2733 2762 2833 3099 3560 4302 4387 Size: 399 943 29 71 266 461 742 85 Cuts at: 4387 5458 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 943 742 461 399 266 85 71 29 BspEI T'CCGG_A Cuts at: 3387 5904 3387 Size: 2517 3724 BspHI T'CATG_A Cuts at: 2443 2976 4130 5860 2443 Size: 533 1154 1730 2824 Fragments arranged by size: 2824 1730 1154 533 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2688 3596 844 Size: 1844 908 3489 Fragments arranged by size: 3489 1844 908 BsrBI CCG'CTC Cuts at: 1394 1823 4128 1394 Size: 429 2305 3507 Fragments arranged by size: 3507 2305 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2719 4736 4910 65 Size: 1183 1471 2017 174 1396 Fragments arranged by size: 2017 1471 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4885 5072 1700 Size: 110 3075 187 2869 Fragments arranged by size: 3075 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2584 2862 96 Size: 2488 278 3475 Fragments arranged by size: 3475 2488 278 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6241 BssSI C'ACGA_G Cuts at: 4301 5313 4301 Size: 1012 5229 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6241 BstBI TT'CG_AA Cuts at: 5045 5045 Size: 6241 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6241 BstXI CCAn_nnnn'nTGG Cuts at: 2849 2849 Size: 6241 BstYI r'GATC_y Cuts at: 1972 3384 3571 3938 4323 4340 5792 5878 5890 Size: 1412 187 367 385 17 1452 86 12 Cuts at: 5890 6236 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1412 385 367 346 187 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2207 2207 Size: 6241 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5689 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5502 Fragments arranged by size: 5502 683 56 BtsI GCAGTG_nn' Cuts at: 3757 4551 4571 3757 Size: 794 20 5427 Fragments arranged by size: 5427 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6206 DraI TTT'AAA Cuts at: 2130 2400 3545 2130 Size: 270 1145 4826 Fragments arranged by size: 4826 1145 270 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5248 809 Size: 507 3932 1802 Fragments arranged by size: 3932 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3688 4577 6206 Size: 54 1425 50 275 18 1858 889 1629 Cuts at: 6206 8 Size: 43 Fragments arranged by size: 1858 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3688 1537 Size: 275 1876 4090 Fragments arranged by size: 4090 1876 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2157 2864 4171 1353 Size: 804 707 1307 3423 Fragments arranged by size: 3423 1307 804 707 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4828 5202 5348 387 Size: 1064 138 3239 374 146 1280 Fragments arranged by size: 3239 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3359 4304 4901 5688 5956 1239 Size: 393 1727 945 597 787 268 1524 Fragments arranged by size: 1727 1524 945 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3259 3259 Size: 6241 EcoRI G'AATT_C Cuts at: 3550 3928 3550 Size: 378 5863 FspI TGC'GCA Cuts at: 3507 4747 3507 Size: 1240 5001 HaeII r_GCGC'y Cuts at: 1678 2020 5388 1678 Size: 342 3368 2531 Fragments arranged by size: 3368 2531 342 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2395 2427 4882 4914 4956 Size: 32 135 32 823 32 2455 32 42 Cuts at: 4956 4988 1373 Size: 32 2626 Fragments arranged by size: 2626 2455 823 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3585 3831 669 Size: 988 281 1647 246 3079 Fragments arranged by size: 3079 1647 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6241 HpaI GTT'AAC Cuts at: 3831 3831 Size: 6241 KpnI G_GTAC'C Cuts at: 3566 3566 Size: 6241 MfeI C'AATT_G Cuts at: 3840 3840 Size: 6241 MluI A'CGCG_T Cuts at: 6117 6117 Size: 6241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2093 2284 5355 5539 1305 Size: 788 191 3071 184 2007 Fragments arranged by size: 3071 2007 788 191 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4419 Fragments arranged by size: 4419 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3080 3462 3498 4199 4558 4717 518 Size: 648 1914 382 36 701 359 159 2042 Fragments arranged by size: 2042 1914 701 648 382 359 159 36 MspA1I CmG'CkG Cuts at: 1606 3657 4334 5482 5727 1606 Size: 2051 677 1148 245 2120 Fragments arranged by size: 2120 2051 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5689 NdeI CA'TA_TG Cuts at: 387 3104 387 Size: 2717 3524 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6241 NotI GC'GGCC_GC Cuts at: 3688 3688 Size: 6241 NspI r_CATG'y Cuts at: 1132 1229 3503 3599 5144 1132 Size: 97 2274 96 1545 2229 Fragments arranged by size: 2274 2229 1545 97 96 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6241 PciI A'CATG_T Cuts at: 5140 5140 Size: 6241 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6241 PfoI T'CCnGG_A Cuts at: 2009 2009 Size: 6241 PmeI GTTT'AAAC Cuts at: 3545 3545 Size: 6241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4310 4342 5823 5855 4310 Size: 32 1481 32 4696 Fragments arranged by size: 4696 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6241 PsiI TTA'TAA Cuts at: 3811 3811 Size: 6241 PstI C_TGCA'G Cuts at: 830 2785 3593 830 Size: 1955 808 3478 Fragments arranged by size: 3478 1955 808 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6241 SalI G'TCGA_C Cuts at: 1936 3583 1936 Size: 1647 4594 SapI GCTCTTCn'nnn_ Cuts at: 2864 2864 Size: 6241 SbfI CC_TGCA'GG Cuts at: 3593 3593 Size: 6241 ScaI AGT'ACT Cuts at: 4489 6201 4489 Size: 1712 4529 SfcI C'TryA_G Cuts at: 826 1045 2770 2781 3153 3589 4724 5405 5596 Size: 219 1725 11 372 436 1135 681 191 Cuts at: 5596 826 Size: 1471 Fragments arranged by size: 1725 1471 1135 681 436 372 219 191 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6241 SmaI CCC'GGG Cuts at: 1872 3350 3568 1872 Size: 1478 218 4545 Fragments arranged by size: 4545 1478 218 SmlI C'TyrA_G Cuts at: 820 1017 1943 3254 4345 5246 5508 820 Size: 197 926 1311 1091 901 262 1553 Fragments arranged by size: 1553 1311 1091 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4032 493 Size: 3539 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6241 SphI G_CATG'C Cuts at: 1132 3599 1132 Size: 2467 3774 SspI AAT'ATT Cuts at: 5 52 2501 4165 5 Size: 47 2449 1664 2081 Fragments arranged by size: 2449 2081 1664 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6241 StyI C'CwwG_G Cuts at: 513 1065 2724 3520 3680 513 Size: 552 1659 796 160 3074 Fragments arranged by size: 3074 1659 796 552 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2330 3432 4268 4285 4438 4623 5068 Size: 1255 750 1102 836 17 153 185 445 Cuts at: 5068 325 Size: 1498 Fragments arranged by size: 1498 1255 1102 836 750 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2584 2862 4487 Size: 274 80 33 51 1443 607 278 1625 Cuts at: 4487 6199 96 Size: 1712 138 Fragments arranged by size: 1712 1625 1443 607 278 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2872 2904 6201 6233 2872 Size: 32 3297 32 2880 Fragments arranged by size: 3297 2880 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2042 1316 Size: 726 5515 XbaI T'CTAG_A Cuts at: 3577 3577 Size: 6241 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3012 3199 3012 Size: 187 6054 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6241 XmaI C'CCGG_G Cuts at: 1870 3348 3566 1870 Size: 1478 218 4545 Fragments arranged by size: 4545 1478 218 XmnI GAAnn'nnTTC Cuts at: 4370 4370 Size: 6241 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4049 276 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI