(Circular) (Six-base) MAPSORT of: pFN21AB4940.seq Check: 7623 from: 1 to: 6142 pFN21AB4940 6142 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2105 2453 3261 2105 Size: 348 808 4986 Fragments arranged by size: 4986 808 348 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3485 1937 Size: 1548 4594 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2717 4205 5589 5857 1632 Size: 1085 1488 1384 268 1917 Fragments arranged by size: 1917 1488 1384 1085 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 5041 6018 1203 Size: 3838 977 1327 Fragments arranged by size: 3838 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 2106 2961 4871 2106 Size: 855 1910 3377 Fragments arranged by size: 3377 1910 855 AleI CACnn'nnGTG Cuts at: 1166 3195 3267 1166 Size: 2029 72 4041 Fragments arranged by size: 4041 2029 72 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2708 1184 Size: 1524 4618 AlwNI CAG_nnn'CTG Cuts at: 2991 3885 5457 2991 Size: 894 1572 3676 Fragments arranged by size: 3676 1572 894 ApaI G_GGCC'C Cuts at: 3042 3042 Size: 6142 ApaLI G'TGCA_C Cuts at: 2857 4199 5355 2857 Size: 1342 1156 3644 Fragments arranged by size: 3644 1342 1156 ApoI r'AATT_y Cuts at: 1493 3311 3451 3677 3829 5938 1493 Size: 1818 140 226 152 2109 1697 Fragments arranged by size: 2109 1818 1697 226 152 140 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 2482 2590 3034 3467 1870 Size: 73 539 108 444 433 4545 Fragments arranged by size: 4545 539 444 433 108 73 BamHI G'GATC_C Cuts at: 3472 3839 3472 Size: 367 5775 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 2269 2491 2794 3360 Size: 325 217 627 330 152 222 303 566 Cuts at: 3360 3463 4918 618 Size: 103 1455 1842 Fragments arranged by size: 1842 1455 627 566 330 325 303 222 217 152 103 BanII G_rGCy'C Cuts at: 1391 2148 2588 2613 3042 3108 3141 3461 1391 Size: 757 440 25 429 66 33 320 4072 Fragments arranged by size: 4072 757 440 429 320 66 33 25 BbeI G_GCGC'C Cuts at: 2273 2495 2798 2273 Size: 222 303 5617 Fragments arranged by size: 5617 303 222 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2105 3163 928 Size: 971 206 1058 3907 Fragments arranged by size: 3907 1058 971 206 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4333 4367 1903 Size: 34 2396 34 3678 Fragments arranged by size: 3678 2396 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 1548 Size: 6142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2246 2592 2772 Size: 107 122 71 631 605 574 346 180 Cuts at: 2772 4753 136 Size: 1981 1525 Fragments arranged by size: 1981 1525 631 605 574 346 180 122 107 71 BglII A'GATC_T Cuts at: 6137 6137 Size: 6142 BlpI GC'TnA_GC Cuts at: 2708 2978 3559 2708 Size: 270 581 5291 Fragments arranged by size: 5291 581 270 Bme1580I G_kGCm'C Cuts at: 1790 2122 2828 2861 3042 3363 4203 5359 1790 Size: 332 706 33 181 321 840 1156 2573 Fragments arranged by size: 2573 1156 840 706 332 321 181 33 BmgBI CAC'GTC Cuts at: 1318 2581 1318 Size: 1263 4879 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2165 3301 3873 Size: 416 53 83 399 24 748 1136 572 Cuts at: 3873 4831 442 Size: 958 1753 Fragments arranged by size: 1753 1136 958 748 572 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2164 2534 2866 2164 Size: 370 332 5440 Fragments arranged by size: 5440 370 332 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 4802 1239 Size: 1007 2556 2579 Fragments arranged by size: 2579 2556 1007 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2987 3227 4267 5132 5430 2987 Size: 240 1040 865 298 3699 Fragments arranged by size: 3699 1040 865 298 240 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2282 2854 4805 882 Size: 617 783 572 1951 2219 Fragments arranged by size: 2219 1951 783 617 572 BsaAI yAC'GTr Cuts at: 493 1204 2823 3933 493 Size: 711 1619 1110 2702 Fragments arranged by size: 2702 1619 1110 711 BsaBI GATnn'nnATC Cuts at: 6136 6136 Size: 6142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2270 2492 2702 2795 Size: 53 83 186 936 737 222 210 93 Cuts at: 2795 3949 4331 4966 275 Size: 1154 382 635 1451 Fragments arranged by size: 1451 1154 936 737 635 382 222 210 186 93 83 53 BsaWI w'CCGG_w Cuts at: 2353 4575 4973 5247 5394 5805 2353 Size: 2222 398 274 147 411 2690 Fragments arranged by size: 2690 2222 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2335 2365 1178 Size: 30 165 30 932 30 4955 Fragments arranged by size: 4955 932 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2309 2386 2507 3149 1186 Size: 970 153 77 121 642 4179 Fragments arranged by size: 4179 970 642 153 121 77 BseYI C'CCAG_C Cuts at: 2939 5345 5841 2939 Size: 2406 496 3240 Fragments arranged by size: 3240 2406 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2892 1163 Size: 573 1156 4413 Fragments arranged by size: 4413 1156 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2185 2211 2903 3164 3391 3592 Size: 275 186 184 26 692 261 227 201 Cuts at: 3592 4353 5381 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 692 275 261 227 201 186 184 26 BsiHKAI G_wGCw'C Cuts at: 2148 2613 2861 3108 4203 4288 5359 2148 Size: 465 248 247 1095 85 1071 2931 Fragments arranged by size: 2931 1095 1071 465 248 247 85 BsmI GAATG_Cn' Cuts at: 3653 3746 3653 Size: 93 6049 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2122 2148 2588 2613 2828 2861 3042 Size: 399 332 26 440 25 215 33 181 Cuts at: 3042 3108 3141 3363 3461 4203 4288 5359 1391 Size: 66 33 222 98 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 440 399 332 222 215 181 98 85 66 33 33 26 25 BspEI T'CCGG_A Cuts at: 2353 5805 2353 Size: 3452 2690 BspHI T'CATG_A Cuts at: 4031 5761 4031 Size: 1730 4412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2105 2453 3261 3497 844 Size: 1261 348 808 236 3489 Fragments arranged by size: 3489 1261 808 348 236 BsrBI CCG'CTC Cuts at: 1394 1823 2232 2537 3091 3321 4029 1394 Size: 429 409 305 554 230 708 3507 Fragments arranged by size: 3507 708 554 429 409 305 230 BsrDI GCAATG_nn' Cuts at: 65 1248 2896 4637 4811 65 Size: 1183 1648 1741 174 1396 Fragments arranged by size: 1741 1648 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2240 2898 2902 4786 4973 1700 Size: 110 430 658 4 1884 187 2869 Fragments arranged by size: 2869 1884 658 430 187 110 4 BsrGI T'GTAC_A Cuts at: 96 2637 96 Size: 2541 3601 BssHII G'CGCG_C Cuts at: 1925 2433 1925 Size: 508 5634 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6142 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstXI CCAn_nnnn'nTGG Cuts at: 2224 2224 Size: 6142 BstYI r'GATC_y Cuts at: 1972 3472 3839 4224 4241 5693 5779 5791 6137 Size: 1500 367 385 17 1452 86 12 346 Cuts at: 6137 1972 Size: 1977 Fragments arranged by size: 1977 1500 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2211 513 Size: 552 1146 4444 Fragments arranged by size: 4444 1146 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5403 Fragments arranged by size: 5403 683 56 BtsI GCAGTG_nn' Cuts at: 2102 3658 4452 4472 2102 Size: 1556 794 20 3772 Fragments arranged by size: 3772 1556 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DraIII CAC_nnn'GTG Cuts at: 2785 2785 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2036 5149 809 Size: 507 720 3113 1802 Fragments arranged by size: 3113 1802 720 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2182 2214 2364 Size: 54 1425 50 275 18 352 32 150 Cuts at: 2364 2604 2618 2893 2900 3589 4478 6107 8 Size: 240 14 275 7 689 889 1629 43 Fragments arranged by size: 1629 1425 889 689 352 275 275 240 150 54 50 43 32 18 14 7 EagI C'GGCC_G Cuts at: 1537 1812 2182 2900 3589 1537 Size: 275 370 718 689 4090 Fragments arranged by size: 4090 718 689 370 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2404 2857 3425 4072 1353 Size: 1051 453 568 647 3423 Fragments arranged by size: 3423 1051 647 568 453 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2250 2523 2582 2634 4729 5103 Size: 1064 138 661 273 59 52 2095 374 Cuts at: 5103 5249 387 Size: 146 1280 Fragments arranged by size: 2095 1280 1064 661 374 273 146 138 59 52 EcoICRI GAG'CTC Cuts at: 2146 2611 3106 2146 Size: 465 495 5182 Fragments arranged by size: 5182 495 465 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 2717 4205 4802 5589 5857 1239 Size: 393 614 471 1488 597 787 268 1524 Fragments arranged by size: 1524 1488 787 614 597 471 393 268 EcoO109I rG'GnC_Cy Cuts at: 3038 3038 Size: 6142 EcoRI G'AATT_C Cuts at: 3451 3829 3451 Size: 378 5764 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 2273 2495 2715 2798 2874 5289 1678 Size: 595 222 220 83 76 2415 2531 Fragments arranged by size: 2531 2415 595 222 220 83 76 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2092 2124 2335 2367 2868 Size: 32 135 32 520 32 211 32 501 Cuts at: 2868 2900 2947 2979 3223 3255 4783 4815 4857 Size: 32 47 32 244 32 1528 32 42 Cuts at: 4857 4889 1373 Size: 32 2626 Fragments arranged by size: 2626 1528 520 501 244 211 135 47 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3486 3732 669 Size: 988 281 1548 246 3079 Fragments arranged by size: 3079 1548 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KasI G'GCGC_C Cuts at: 2269 2491 2794 2269 Size: 222 303 5617 Fragments arranged by size: 5617 303 222 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2570 3387 5256 5440 1305 Size: 1265 817 1869 184 2007 Fragments arranged by size: 2007 1869 1265 817 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2216 2895 10 Size: 54 1425 343 384 679 3257 Fragments arranged by size: 3257 1425 679 384 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3195 3267 4100 4459 4618 518 Size: 648 2029 72 833 359 159 2042 Fragments arranged by size: 2042 2029 833 648 359 159 72 MspA1I CmG'CkG Cuts at: 1606 2133 2421 3558 4235 5383 5628 1606 Size: 527 288 1137 677 1148 245 2120 Fragments arranged by size: 2120 1148 1137 677 527 288 245 NaeI GCC'GGC Cuts at: 2904 2904 Size: 6142 NarI GG'CG_CC Cuts at: 2270 2492 2795 2270 Size: 222 303 5617 Fragments arranged by size: 5617 303 222 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5590 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NgoMIV G'CCGG_C Cuts at: 2902 2902 Size: 6142 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 3130 3500 5045 1132 Size: 97 1901 370 1545 2229 Fragments arranged by size: 2229 1901 1545 370 97 PasI CC'CwG_GG Cuts at: 1348 2923 2959 1348 Size: 1575 36 4531 Fragments arranged by size: 4531 1575 36 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2785 1108 Size: 1677 4465 PfoI T'CCnGG_A Cuts at: 2310 2310 Size: 6142 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PmlI CAC'GTG Cuts at: 2823 2823 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PspOMI G'GGCC_C Cuts at: 3038 3038 Size: 6142 PstI C_TGCA'G Cuts at: 830 2450 3494 830 Size: 1620 1044 3478 Fragments arranged by size: 3478 1620 1044 PvuI CG_AT'CG Cuts at: 2001 3164 2001 Size: 1163 4979 SacI G_AGCT'C Cuts at: 2148 2613 3108 2148 Size: 465 495 5182 Fragments arranged by size: 5182 495 465 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 4390 6102 4390 Size: 1712 4430 SfcI C'TryA_G Cuts at: 826 1045 2446 3490 4625 5306 5497 826 Size: 219 1401 1044 1135 681 191 1471 Fragments arranged by size: 1471 1401 1135 1044 681 219 191 SfoI GGC'GCC Cuts at: 2271 2493 2796 2271 Size: 222 303 5617 Fragments arranged by size: 5617 303 222 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 2484 3036 3469 1872 Size: 612 552 433 4545 Fragments arranged by size: 4545 612 552 433 SmlI C'TyrA_G Cuts at: 820 1017 1943 2966 3242 4246 5147 5409 820 Size: 197 926 1023 276 1004 901 262 1553 Fragments arranged by size: 1553 1023 1004 926 901 276 262 197 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 3130 3500 1132 Size: 1998 370 3774 Fragments arranged by size: 3774 1998 370 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 2514 1517 Size: 997 5145 StyI C'CwwG_G Cuts at: 513 1065 3581 513 Size: 552 2516 3074 Fragments arranged by size: 3074 2516 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2306 2731 2876 3203 3405 4169 4186 Size: 1255 726 425 145 327 202 764 17 Cuts at: 4186 4339 4524 4969 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 764 726 445 425 327 202 185 153 145 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2637 4388 6100 Size: 274 80 33 51 1443 660 1751 1712 Cuts at: 6100 96 Size: 138 Fragments arranged by size: 1751 1712 1443 660 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2901 2933 6102 6134 2901 Size: 32 3169 32 2909 Fragments arranged by size: 3169 2909 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2870 2870 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 2482 3034 3467 1870 Size: 612 552 433 4545 Fragments arranged by size: 4545 612 552 433 XmnI GAAnn'nnTTC Cuts at: 4271 4271 Size: 6142 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI