(Circular) (Six-base) MAPSORT of: pFN21AB4806.seq Check: 7602 from: 1 to: 8116 pFN21AB4806 8116 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3259 3259 Size: 8116 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5926 278 Size: 53 83 186 936 4390 2468 Fragments arranged by size: 4390 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3232 5459 1937 Size: 1295 2227 4594 Fragments arranged by size: 4594 2227 1295 Acc65I G'GTAC_C Cuts at: 2172 2471 5437 2172 Size: 299 2966 4851 Fragments arranged by size: 4851 2966 299 AclI AA'CG_TT Cuts at: 1563 6243 6616 1563 Size: 4680 373 3063 Fragments arranged by size: 4680 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2900 4049 4901 6179 7563 7831 1632 Size: 1268 1149 852 1278 1384 268 1917 Fragments arranged by size: 1917 1384 1278 1268 1149 852 268 AfeI AGC'GCT Cuts at: 3004 3004 Size: 8116 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7919 AflIII A'CryG_T Cuts at: 1203 4959 7015 7992 1203 Size: 3756 2056 977 1327 Fragments arranged by size: 3756 2056 1327 977 AgeI A'CCGG_T Cuts at: 2468 6947 2468 Size: 4479 3637 AhdI GACnn_n'nnGTC Cuts at: 6845 6845 Size: 8116 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8116 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8116 AlwNI CAG_nnn'CTG Cuts at: 2438 2723 2878 2922 3124 3385 3556 3661 4006 Size: 285 155 44 202 261 171 105 345 Cuts at: 4006 4565 5002 5345 5859 7431 2438 Size: 559 437 343 514 1572 3123 Fragments arranged by size: 3123 1572 559 514 437 345 343 285 261 202 171 155 105 44 ApaI G_GGCC'C Cuts at: 3053 3835 4374 3053 Size: 782 539 6795 Fragments arranged by size: 6795 782 539 ApaLI G'TGCA_C Cuts at: 6173 7329 6173 Size: 1156 6960 ApoI r'AATT_y Cuts at: 1493 2026 5425 5651 5803 7912 1493 Size: 533 3399 226 152 2109 1697 Fragments arranged by size: 3399 2109 1697 533 226 152 AseI AT'TA_AT Cuts at: 160 1032 6670 160 Size: 872 5638 1606 Fragments arranged by size: 5638 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8116 AvaI C'yCGr_G Cuts at: 1870 1943 2067 2193 2425 2445 3089 5441 1870 Size: 73 124 126 232 20 644 2352 4545 Fragments arranged by size: 4545 2352 644 232 126 124 73 20 AvrII C'CTAG_G Cuts at: 4970 4970 Size: 8116 BamHI G'GATC_C Cuts at: 2856 3437 5446 5813 2856 Size: 581 2009 367 5159 Fragments arranged by size: 5159 2009 581 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2172 2214 2471 2632 5437 Size: 325 217 627 385 42 257 161 2805 Cuts at: 5437 6892 618 Size: 1455 1842 Fragments arranged by size: 2805 1842 1455 627 385 325 257 217 161 42 BanII G_rGCy'C Cuts at: 1391 2576 2799 2840 2904 2969 3053 3835 4374 Size: 1185 223 41 64 65 84 782 539 Cuts at: 4374 5435 1391 Size: 1061 4072 Fragments arranged by size: 4072 1185 1061 782 539 223 84 65 64 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2957 3795 4538 4553 4962 928 Size: 971 1058 838 743 15 409 4082 Fragments arranged by size: 4082 1058 971 838 743 409 15 BbvCI CC'TCA_GC Cuts at: 2161 3797 2161 Size: 1636 6480 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6307 6341 1903 Size: 34 4370 34 3678 Fragments arranged by size: 4370 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6008 7218 6008 Size: 1210 6906 BclI T'GATC_A Cuts at: 1548 1548 Size: 8116 BfrBI ATG'CAT Cuts at: 3368 3368 Size: 8116 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2448 4410 6727 Size: 107 122 71 631 605 776 1962 2317 Cuts at: 6727 136 Size: 1525 Fragments arranged by size: 2317 1962 1525 776 631 605 122 107 71 BglII A'GATC_T Cuts at: 3484 8111 3484 Size: 4627 3489 BlpI GC'TnA_GC Cuts at: 2839 2849 3750 5533 2839 Size: 10 901 1783 5422 Fragments arranged by size: 5422 1783 901 10 Bme1580I G_kGCm'C Cuts at: 1790 3053 3835 4374 5124 6177 7333 1790 Size: 1263 782 539 750 1053 1156 2573 Fragments arranged by size: 2573 1263 1156 1053 782 750 539 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8116 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3216 4228 4320 Size: 416 53 83 399 24 1799 1012 92 Cuts at: 4320 5847 6805 442 Size: 1527 958 1753 Fragments arranged by size: 1799 1753 1527 1012 958 416 399 92 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8116 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2103 2759 3988 5041 2103 Size: 656 1229 1053 5178 Fragments arranged by size: 5178 1229 1053 656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2091 2170 2792 3362 3760 4076 4082 4091 Size: 852 79 622 570 398 316 6 9 Cuts at: 4091 4631 4709 4717 4846 5012 5024 6776 1239 Size: 540 78 8 129 166 12 1752 2579 Fragments arranged by size: 2579 1752 852 622 570 540 398 316 166 129 79 78 12 9 8 6 Bpu10I CC'TnA_GC Cuts at: 2161 3797 2161 Size: 1636 6480 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2189 2229 4103 4588 5243 6241 7106 7404 2189 Size: 40 1874 485 655 998 865 298 2901 Fragments arranged by size: 2901 1874 998 865 655 485 298 40 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2091 3295 4283 5105 6779 882 Size: 617 592 1204 988 822 1674 2219 Fragments arranged by size: 2219 1674 1204 988 822 617 592 BsaAI yAC'GTr Cuts at: 493 1204 4618 5907 493 Size: 711 3414 1289 2702 Fragments arranged by size: 3414 2702 1289 711 BsaBI GATnn'nnATC Cuts at: 4820 8110 4820 Size: 3290 4826 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2340 2451 2534 5923 Size: 53 83 186 936 807 111 83 3389 Cuts at: 5923 6305 6940 275 Size: 382 635 1451 Fragments arranged by size: 3389 1451 936 807 635 382 186 111 83 83 53 BsaWI w'CCGG_w Cuts at: 2468 2679 5097 6549 6947 7221 7368 7779 2468 Size: 211 2418 1452 398 274 147 411 2805 Fragments arranged by size: 2805 2418 1452 411 398 274 211 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2174 2204 4757 4787 5030 Size: 30 165 30 771 30 2553 30 243 Cuts at: 5030 5060 1178 Size: 30 4234 Fragments arranged by size: 4234 2553 771 243 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2741 2744 2990 4913 1186 Size: 1006 549 3 246 1923 4389 Fragments arranged by size: 4389 1923 1006 549 246 3 BseYI C'CCAG_C Cuts at: 2177 2221 2609 2624 2782 2798 2907 2915 3117 Size: 44 388 15 158 16 109 8 202 Cuts at: 3117 3280 3549 4040 4519 7319 7815 2177 Size: 163 269 491 479 2800 496 2478 Fragments arranged by size: 2800 2478 496 491 479 388 269 202 163 158 109 44 16 15 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7543 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5566 6327 7355 1540 Size: 275 186 3565 761 1028 2301 Fragments arranged by size: 3565 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2202 2417 2840 4617 6177 6262 7333 2202 Size: 215 423 1777 1560 85 1071 2985 Fragments arranged by size: 2985 1777 1560 1071 423 215 85 BsmI GAATG_Cn' Cuts at: 3840 5627 5720 3840 Size: 1787 93 6236 Fragments arranged by size: 6236 1787 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2331 2331 Size: 8116 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2417 2576 2799 2840 2904 2969 Size: 399 412 215 159 223 41 64 65 Cuts at: 2969 3053 3835 4374 4617 5124 5435 6177 6262 Size: 84 782 539 243 507 311 742 85 Cuts at: 6262 7333 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 782 742 539 507 412 399 311 243 223 215 159 85 84 65 64 41 BspEI T'CCGG_A Cuts at: 2679 7779 2679 Size: 5100 3016 BspHI T'CATG_A Cuts at: 4491 6005 7735 4491 Size: 1514 1730 4872 Fragments arranged by size: 4872 1730 1514 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2073 2160 3259 4649 5471 844 Size: 1229 87 1099 1390 822 3489 Fragments arranged by size: 3489 1390 1229 1099 822 87 BsrBI CCG'CTC Cuts at: 1394 1823 2143 4084 6003 1394 Size: 429 320 1941 1919 3507 Fragments arranged by size: 3507 1941 1919 429 320 BsrDI GCAATG_nn' Cuts at: 65 1248 4576 6611 6785 65 Size: 1183 3328 2035 174 1396 Fragments arranged by size: 3328 2035 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2211 2468 2766 2980 3696 4455 6760 Size: 110 401 257 298 214 716 759 2305 Cuts at: 6760 6947 1700 Size: 187 2869 Fragments arranged by size: 2869 2305 759 716 401 298 257 214 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8116 BssHII G'CGCG_C Cuts at: 1925 4033 1925 Size: 2108 6008 BssSI C'ACGA_G Cuts at: 6176 7188 6176 Size: 1012 7104 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8116 BstBI TT'CG_AA Cuts at: 6920 6920 Size: 8116 BstEII G'GTnAC_C Cuts at: 1184 3323 1184 Size: 2139 5977 BstXI CCAn_nnnn'nTGG Cuts at: 2048 2048 Size: 8116 BstYI r'GATC_y Cuts at: 1972 2248 2856 3437 3484 4464 4922 5446 5813 Size: 276 608 581 47 980 458 524 367 Cuts at: 5813 6198 6215 7667 7753 7765 8111 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 980 608 581 524 458 385 367 346 276 86 47 17 12 Bsu36I CC'TnA_GG Cuts at: 2810 3600 2810 Size: 790 7326 BtgI C'CryG_G Cuts at: 513 1065 2041 4596 513 Size: 552 976 2555 4033 Fragments arranged by size: 4033 2555 976 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4116 487 Size: 683 56 2890 4487 Fragments arranged by size: 4487 2890 683 56 BtsI GCAGTG_nn' Cuts at: 2850 3079 3673 4291 4679 5632 6426 6446 2850 Size: 229 594 618 388 953 794 20 4520 Fragments arranged by size: 4520 953 794 618 594 388 229 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8116 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3249 3284 529 Size: 35 2685 35 5361 Fragments arranged by size: 5361 2685 35 35 DraI TTT'AAA Cuts at: 5420 5420 Size: 8116 DraIII CAC_nnn'GTG Cuts at: 4750 5147 4750 Size: 397 7719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7123 809 Size: 507 5807 1802 Fragments arranged by size: 5807 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2592 5563 6452 Size: 54 1425 50 275 18 762 2971 889 Cuts at: 6452 8081 8 Size: 1629 43 Fragments arranged by size: 2971 1629 1425 889 762 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5563 1537 Size: 275 3751 4090 Fragments arranged by size: 4090 3751 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3128 3400 3526 3683 3890 6046 1353 Size: 1775 272 126 157 207 2156 3423 Fragments arranged by size: 3423 2156 1775 272 207 157 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5201 6703 7077 7223 387 Size: 1064 138 3612 1502 374 146 1280 Fragments arranged by size: 3612 1502 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2838 2838 Size: 8116 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2091 2170 2792 2900 3362 3760 4049 Size: 393 459 79 622 108 462 398 289 Cuts at: 4049 4076 4082 4091 4631 4709 4717 4846 4901 Size: 27 6 9 540 78 8 129 55 Cuts at: 4901 5012 5024 6179 6776 7563 7831 1239 Size: 111 12 1155 597 787 268 1524 Fragments arranged by size: 1524 1155 787 622 597 540 462 459 398 393 289 268 129 111 108 79 78 55 27 12 9 8 6 EcoNI CCTnn'n_nnAGG Cuts at: 2809 4442 2809 Size: 1633 6483 EcoO109I rG'GnC_Cy Cuts at: 2674 2694 2875 3093 4370 4371 4435 5163 5296 Size: 20 181 218 1277 1 64 728 133 Cuts at: 5296 2674 Size: 5494 Fragments arranged by size: 5494 1277 728 218 181 133 64 20 1 EcoRI G'AATT_C Cuts at: 2026 5425 5803 2026 Size: 3399 378 4339 Fragments arranged by size: 4339 3399 378 EcoRV GAT'ATC Cuts at: 2508 2508 Size: 8116 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3199 3199 Size: 8116 FspI TGC'GCA Cuts at: 3665 6622 3665 Size: 2957 5159 HaeII r_GCGC'y Cuts at: 1678 2744 2805 3006 4006 4351 4456 4696 4907 Size: 1066 61 201 1000 345 105 240 211 Cuts at: 4907 7263 1678 Size: 2356 2531 Fragments arranged by size: 2531 2356 1066 1000 345 240 211 201 105 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2492 2524 3353 3385 3580 Size: 32 135 32 920 32 829 32 195 Cuts at: 3580 3612 3803 3835 4900 4932 5030 5062 6757 Size: 32 191 32 1065 32 98 32 1695 Cuts at: 6757 6789 6831 6863 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1695 1065 920 829 195 191 135 98 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5460 5706 669 Size: 988 281 3522 246 3079 Fragments arranged by size: 3522 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3848 748 Size: 3100 5016 HpaI GTT'AAC Cuts at: 5706 5706 Size: 8116 KpnI G_GTAC'C Cuts at: 2176 2475 5441 2176 Size: 299 2966 4851 Fragments arranged by size: 4851 2966 299 MfeI C'AATT_G Cuts at: 5715 5715 Size: 8116 MluI A'CGCG_T Cuts at: 7992 7992 Size: 8116 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3001 4963 5064 5073 7230 7414 1305 Size: 1696 1962 101 9 2157 184 2007 Fragments arranged by size: 2157 2007 1962 1696 184 101 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6294 Fragments arranged by size: 6294 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3726 4541 4706 6074 6433 6592 Size: 648 843 1717 815 165 1368 359 159 Cuts at: 6592 518 Size: 2042 Fragments arranged by size: 2042 1717 1368 843 815 648 359 165 159 MspA1I CmG'CkG Cuts at: 1606 2438 2922 3121 3152 3754 4472 4737 5532 Size: 832 484 199 31 602 718 265 795 Cuts at: 5532 6209 7357 7602 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 832 795 718 677 602 484 265 245 199 31 NaeI GCC'GGC Cuts at: 2982 4457 2982 Size: 1475 6641 NcoI C'CATG_G Cuts at: 513 1065 4596 513 Size: 552 3531 4033 Fragments arranged by size: 4033 3531 552 NdeI CA'TA_TG Cuts at: 387 3505 387 Size: 3118 4998 NgoMIV G'CCGG_C Cuts at: 2980 4455 2980 Size: 1475 6641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8116 NotI GC'GGCC_GC Cuts at: 5563 5563 Size: 8116 NruI TCG'CGA Cuts at: 4135 4135 Size: 8116 NsiI A_TGCA'T Cuts at: 3370 3370 Size: 8116 NspI r_CATG'y Cuts at: 1132 1229 2554 3319 3739 5474 7019 1132 Size: 97 1325 765 420 1735 1545 2229 Fragments arranged by size: 2229 1735 1545 1325 765 420 97 PasI CC'CwG_GG Cuts at: 1348 4526 1348 Size: 3178 4938 PciI A'CATG_T Cuts at: 7015 7015 Size: 8116 PflMI CCAn_nnn'nTGG Cuts at: 1108 2438 2488 4260 4565 4750 1108 Size: 1330 50 1772 305 185 4474 Fragments arranged by size: 4474 1772 1330 305 185 50 PfoI T'CCnGG_A Cuts at: 2496 2751 2496 Size: 255 7861 PmeI GTTT'AAAC Cuts at: 5420 5420 Size: 8116 PmlI CAC'GTG Cuts at: 4618 4618 Size: 8116 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6185 6217 7698 7730 6185 Size: 32 1481 32 6571 Fragments arranged by size: 6571 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2694 2875 3093 4435 2694 Size: 181 218 1342 6375 Fragments arranged by size: 6375 1342 218 181 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8116 PsiI TTA'TAA Cuts at: 5686 5686 Size: 8116 PspOMI G'GGCC_C Cuts at: 3049 3831 4370 3049 Size: 782 539 6795 Fragments arranged by size: 6795 782 539 PstI C_TGCA'G Cuts at: 830 2171 2648 4346 5468 830 Size: 1341 477 1698 1122 3478 Fragments arranged by size: 3478 1698 1341 1122 477 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8116 PvuII CAG'CTG Cuts at: 2438 2922 3754 4737 2438 Size: 484 832 983 5817 Fragments arranged by size: 5817 983 832 484 SacI G_AGCT'C Cuts at: 2840 2840 Size: 8116 SalI G'TCGA_C Cuts at: 1936 5458 1936 Size: 3522 4594 SanDI GG'GwC_CC Cuts at: 3093 3093 Size: 8116 SapI GCTCTTCn'nnn_ Cuts at: 3526 3683 3526 Size: 157 7959 SbfI CC_TGCA'GG Cuts at: 2171 5468 2171 Size: 3297 4819 ScaI AGT'ACT Cuts at: 4100 5324 6364 8076 4100 Size: 1224 1040 1712 4140 Fragments arranged by size: 4140 1712 1224 1040 SfcI C'TryA_G Cuts at: 826 1045 2167 2644 2926 4342 5464 6599 7280 Size: 219 1122 477 282 1416 1122 1135 681 Cuts at: 7280 7471 826 Size: 191 1471 Fragments arranged by size: 1471 1416 1135 1122 1122 681 477 282 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8116 SgrAI Cr'CCGG_yG Cuts at: 1700 4455 1700 Size: 2755 5361 SmaI CCC'GGG Cuts at: 1872 2195 2427 2447 3091 5443 1872 Size: 323 232 20 644 2352 4545 Fragments arranged by size: 4545 2352 644 323 232 20 SmlI C'TyrA_G Cuts at: 820 1017 1943 2204 2244 4118 4603 5222 6220 Size: 197 926 261 40 1874 485 619 998 Cuts at: 6220 7121 7383 820 Size: 901 262 1553 Fragments arranged by size: 1874 1553 998 926 901 619 485 262 261 197 40 SnaBI TAC'GTA Cuts at: 493 5907 493 Size: 5414 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8116 SphI G_CATG'C Cuts at: 1132 5474 1132 Size: 4342 3774 SspI AAT'ATT Cuts at: 5 52 6040 5 Size: 47 5988 2081 Fragments arranged by size: 5988 2081 47 StuI AGG'CCT Cuts at: 1517 5180 1517 Size: 3663 4453 StyI C'CwwG_G Cuts at: 513 1065 2123 2359 2961 2970 3492 3570 4254 Size: 552 1058 236 602 9 522 78 684 Cuts at: 4254 4408 4431 4596 4970 5555 513 Size: 154 23 165 374 585 3074 Fragments arranged by size: 3074 1058 684 602 585 552 522 374 236 165 154 78 23 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2054 2287 3259 4197 4944 6143 6160 Size: 1255 474 233 972 938 747 1199 17 Cuts at: 6160 6313 6498 6943 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1199 972 938 747 474 445 233 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3612 4098 4389 Size: 274 80 33 51 1443 1635 486 291 Cuts at: 4389 5322 6362 8074 96 Size: 933 1040 1712 138 Fragments arranged by size: 1712 1635 1443 1040 933 486 291 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2174 2206 3317 3349 4613 4645 4777 4809 8076 Size: 32 1111 32 1264 32 132 32 3267 Cuts at: 8076 8108 2174 Size: 32 2182 Fragments arranged by size: 3267 2182 1264 1111 132 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4823 1316 Size: 3507 4609 XbaI T'CTAG_A Cuts at: 5452 5452 Size: 8116 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2048 3431 4843 2048 Size: 1383 1412 5321 Fragments arranged by size: 5321 1412 1383 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8116 XmaI C'CCGG_G Cuts at: 1870 2193 2425 2445 3089 5441 1870 Size: 323 232 20 644 2352 4545 Fragments arranged by size: 4545 2352 644 323 232 20 XmnI GAAnn'nnTTC Cuts at: 2320 4550 6245 2320 Size: 2230 1695 4191 Fragments arranged by size: 4191 2230 1695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5924 276 Size: 53 83 186 936 4390 2468 Fragments arranged by size: 4390 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BsiWI BstZ17I FseI FspAI KasI NarI PacI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI