Restriction map for
pFN21AB4782
##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp718I AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BfrI
BglI BlnI BlpI Bpu1102I BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BsrDI BsrFI BssHII
Bst1107I BstBI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
FauI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tsp509I
XbaI
###################################
# ONE CUT ENZYMES ( 70 enzymes) #
###################################
AhdI 787 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 997 GTGCAC G^TGCAC
AlwNI 52 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 997 GTGCAC G^TGCAC
Asp700I 858 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 787 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 367 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1007 TGGCCA TGG^CCA
BamHI 755 GGATCC G^GATCC
BanII 834 GAGCCC G[GA]GC[CT]^C
BciVI 991 GGATAC GTATCC(6/5)
BclI 500 TGATCA T^GATCA
BfaI 721 CTAG C^TAG
BglII 332 AGATCT A^GATCT
BsaAI 994 TACGTG [CT]AC^GT[GA]
BsaBI 1017 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 747 ACCGGA [AT]^CCGG[AT]
BseRI 46 GAGGAG GAGGAG(10/8)
BsmI 591 GCATTC GAATGC(1/-1)
Bsp1407I 278 TGTACA T^GTACA
BspLU11I 740 ACATGT A^CATGT
BspMI 289 ACCTGC ACCTGC(4/8)
BsrGI 278 TGTACA T^GTACA
BsrI 942 ACTGG ACTGG(1/-1)
BssSI 82 CTCGTG CACGAG(-5/-1)
BstAPI 51 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 667 GGTGACC G^GT[ACGT]ACC
BstPI 667 GGTGACC G^GT[ACGT]ACC
CfoI 575 GCGC GCG^C
DdeI 698 CTGAG C^T[ACGT]AG
DraIII 1000 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 787 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 862 CTCTTC CTCTTC(1/4)
EcoO65I 667 GGTGACC G^GT[ACGT]ACC
EcoT22I 589 ATGCAT ATGCA^T
FbaI 500 TGATCA T^GATCA
HaeII 574 AGCGCC [GA]GCGC^[CT]
HhaI 575 GCGC GCG^C
HinP1I 575 GCGC G^CGC
Ksp632I 862 CTCTTC CTCTTC(1/4)
MaeI 721 CTAG C^TAG
MamI 1017 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1007 TGGCCA TGG^CCA
MscI 1007 TGGCCA TGG^CCA
MseI 512 TTAA T^TAA
MslI 1023 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 565 CAGCTG C[AC]G^C[GT]G
NcoI 129 CCATGG C^CATGG
NsiI 589 ATGCAT ATGCA^T
PaeR7I 68 CTCGAG C^TCGAG
PflFI 367 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 156 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 430 GACTC GAGTC(4/5)
PpuMI 485 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 670 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 565 CAGCTG CAG^CTG
SexAI 1001 ACCAGGT A^CC[AT]GGT
SmaI 910 CCCGGG CCC^GGG
SmlI 68 CTCGAG C^T[CT][GA]AG
SphI 94 GCATGC GCATG^C
SspBI 278 TGTACA T^GTACA
StuI 64 AGGCCT AGG^CCT
TfiI 858 GAATC G^A[AT]TC
Tru9I 512 TTAA T^TAA
Tth111I 367 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 156 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 68 CTCGAG C^TCGAG
XmaCI 910 CCCGGG C^CCGGG
XmaI 910 CCCGGG C^CCGGG
XmnI 858 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 632 ACGTGT A^C[GA][CT]GT
740 ACATGT
AlwI 756 GATCC GGATC(4/5)
755 GGATC
AvaI 68 CTCGAG C^[CT]CG[GA]G
910 CCCGGG
AvaII 486 GGACC G^G[AT]CC
794 GGTCC
BbsI 306 GAAGAC GAAGAC(2/6)
442 GAAGAC
BpmI 829 CTCCAG CTGGAG(16/14)
466 CTGGAG
BpuAI 306 GAAGAC GAAGAC(2/6)
442 GAAGAC
BsaI 700 GAGACC GGTCTC(1/5)
978 GAGACC
BsmFI 485 GGGAC GGGAC(10/14)
727 GGGAC
BsoBI 68 CTCGAG C^[CT]CG[GA]G
910 CCCGGG
BstYI 332 AGATCT [GA]^GATC[CT]
755 GGATCC
DraII 165 GGGGCCT [GA]G^G[ACGT]CC[CT]
485 GGGACCT
EaeI 117 CGGCCA [CT]^GGCC[GA]
1007 TGGCCA
EcoO109I 165 GGGGCCT [GA]G^G[ACGT]CC[CT]
485 GGGACCT
HinfI 430 GACTC G^A[ACGT]TC
858 GAATC
MaeII 632 ACGT A^CGT
995 ACGT
MflI 332 AGATCT [GA]^GATC[CT]
755 GGATCC
ScaI 506 AGTACT AGT^ACT
554 AGTACT
TspRI 311 CAGTG CA[GC]TG[ACGT][ACGT]^
889 CAGTG
XcmI 120 CCACATGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
287 CCACCTGCCTGCTGG
XhoII 332 AGATCT [GA]^GATC[CT]
755 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
BbvI BcnI BmyI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HpaII HphI ItaI MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI Tsp45I
TthHB8I