(Circular) (Six-base) MAPSORT of: pFN21AB4782.seq Check: 5641 from: 1 to: 5746 pFN21AB4782 5746 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2303 2303 Size: 5746 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3089 1937 Size: 1152 4594 Acc65I G'GTAC_C Cuts at: 3067 3067 Size: 5746 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 2680 3809 5193 5461 1632 Size: 713 335 1129 1384 268 1917 Fragments arranged by size: 1917 1384 1129 713 335 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 2637 2745 4645 5622 1203 Size: 1434 108 1900 977 1327 Fragments arranged by size: 1900 1434 1327 977 108 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 2797 4475 2797 Size: 1678 4068 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5746 AlwNI CAG_nnn'CTG Cuts at: 2062 3489 5061 2062 Size: 1427 1572 2747 Fragments arranged by size: 2747 1572 1427 ApaLI G'TGCA_C Cuts at: 3002 3803 4959 3002 Size: 801 1156 3789 Fragments arranged by size: 3789 1156 801 ApoI r'AATT_y Cuts at: 1493 3055 3281 3433 5542 1493 Size: 1562 226 152 2109 1697 Fragments arranged by size: 2109 1697 1562 226 152 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2915 3071 1870 Size: 73 130 842 156 4545 Fragments arranged by size: 4545 842 156 130 73 BamHI G'GATC_C Cuts at: 2760 3076 3443 2760 Size: 316 367 5063 Fragments arranged by size: 5063 367 316 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3067 4522 618 Size: 325 217 627 1280 1455 1842 Fragments arranged by size: 1842 1455 1280 627 325 217 BanII G_rGCy'C Cuts at: 1391 2843 3065 1391 Size: 1452 222 4072 Fragments arranged by size: 4072 1452 222 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2318 2454 928 Size: 971 419 136 4220 Fragments arranged by size: 4220 971 419 136 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3937 3971 1903 Size: 34 50 34 1916 34 3678 Fragments arranged by size: 3678 1916 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2990 3638 4848 2990 Size: 648 1210 3888 Fragments arranged by size: 3888 1210 648 BclI T'GATC_A Cuts at: 1548 2505 1548 Size: 957 4789 BfrBI ATG'CAT Cuts at: 2596 2596 Size: 5746 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4357 136 Size: 107 122 71 631 605 2685 1525 Fragments arranged by size: 2685 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2337 5741 2337 Size: 3404 2342 BlpI GC'TnA_GC Cuts at: 3163 3163 Size: 5746 Bme1580I G_kGCm'C Cuts at: 1790 2086 3006 3807 4963 1790 Size: 296 920 801 1156 2573 Fragments arranged by size: 2573 1156 920 801 296 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4435 442 Size: 416 53 83 399 24 2060 958 1753 Fragments arranged by size: 2060 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2944 2944 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2492 2819 4406 1239 Size: 1253 327 1587 2579 Fragments arranged by size: 2579 1587 1253 327 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3871 4736 5034 3871 Size: 865 298 4583 Fragments arranged by size: 4583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2699 2977 4409 882 Size: 617 1200 278 1432 2219 Fragments arranged by size: 2219 1432 1200 617 278 BsaAI yAC'GTr Cuts at: 493 1204 3001 3537 493 Size: 711 1797 536 2702 Fragments arranged by size: 2702 1797 711 536 BsaBI GATnn'nnATC Cuts at: 3026 5740 3026 Size: 2714 3032 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2355 2658 2735 3553 Size: 53 83 186 936 822 303 77 818 Cuts at: 3553 3935 4570 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 822 818 635 382 303 186 83 77 53 BsaWI w'CCGG_w Cuts at: 2752 4179 4577 4851 4998 5409 2752 Size: 1427 398 274 147 411 3089 Fragments arranged by size: 3089 1427 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5521 Fragments arranged by size: 5521 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 1186 Size: 880 4866 BseYI C'CCAG_C Cuts at: 2025 4949 5445 2025 Size: 2924 496 2326 Fragments arranged by size: 2924 2326 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2354 2588 2596 1163 Size: 573 618 234 8 4313 Fragments arranged by size: 4313 618 573 234 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2592 2925 3006 3807 3892 4963 2592 Size: 333 81 801 85 1071 3375 Fragments arranged by size: 3375 1071 801 333 85 81 BsmI GAATG_Cn' Cuts at: 2596 3257 3350 2596 Size: 661 93 4992 Fragments arranged by size: 4992 661 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2592 2843 2925 3006 3065 3807 Size: 399 296 506 251 82 81 59 742 Cuts at: 3807 3892 4963 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 506 399 296 251 85 82 81 59 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2303 3101 844 Size: 1459 798 3489 Fragments arranged by size: 3489 1459 798 BsrBI CCG'CTC Cuts at: 1394 1823 3633 1394 Size: 429 1810 3507 Fragments arranged by size: 3507 1810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4241 4415 65 Size: 1183 2993 174 1396 Fragments arranged by size: 2993 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4390 4577 1700 Size: 110 2580 187 2869 Fragments arranged by size: 2869 2580 187 110 BsrGI T'GTAC_A Cuts at: 96 2283 96 Size: 2187 3559 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 2087 3806 4818 2087 Size: 1719 1012 3015 Fragments arranged by size: 3015 1719 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2062 794 Size: 1268 4478 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 2672 1184 Size: 1488 4258 BstYI r'GATC_y Cuts at: 1972 2337 2760 3076 3443 3828 3845 5297 5383 Size: 365 423 316 367 385 17 1452 86 Cuts at: 5383 5395 5741 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 423 385 367 365 346 316 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2134 2583 2652 513 Size: 552 1069 449 69 3607 Fragments arranged by size: 3607 1069 552 449 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5007 Fragments arranged by size: 5007 683 56 BtsI GCAGTG_nn' Cuts at: 2900 3262 4056 4076 2900 Size: 362 794 20 4570 Fragments arranged by size: 4570 794 362 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DraIII CAC_nnn'GTG Cuts at: 3010 3010 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2122 3012 3193 Size: 54 1425 50 275 18 292 890 181 Cuts at: 3193 4082 5711 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 890 889 292 275 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2873 3676 1353 Size: 1520 803 3423 Fragments arranged by size: 3423 1520 803 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 4333 4707 4853 387 Size: 1064 138 660 2084 374 146 1280 Fragments arranged by size: 2084 1280 1064 660 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2492 2680 2819 3809 4406 5193 Size: 393 713 147 188 139 990 597 787 Cuts at: 5193 5461 1239 Size: 268 1524 Fragments arranged by size: 1524 990 787 713 597 393 268 188 147 139 EcoO109I rG'GnC_Cy Cuts at: 2171 2491 2171 Size: 320 5426 EcoRI G'AATT_C Cuts at: 3055 3433 3055 Size: 378 5368 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 2583 4893 1678 Size: 905 2310 2531 Fragments arranged by size: 2531 2310 905 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2305 2337 2783 2815 2934 Size: 32 135 32 733 32 446 32 119 Cuts at: 2934 2966 2976 3008 4387 4419 4461 4493 1373 Size: 32 10 32 1379 32 42 32 2626 Fragments arranged by size: 2626 1379 733 446 135 119 42 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 3090 3336 669 Size: 988 281 1152 246 3079 Fragments arranged by size: 3079 1152 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 3071 3071 Size: 5746 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2172 2814 4860 5044 1305 Size: 867 642 2046 184 2007 Fragments arranged by size: 2046 2007 867 642 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3014 10 Size: 54 1425 343 1182 2742 Fragments arranged by size: 2742 1425 1182 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3032 3704 4063 4222 518 Size: 648 1866 672 359 159 2042 Fragments arranged by size: 2042 1866 672 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2572 3162 3839 4987 5232 1606 Size: 966 590 677 1148 245 2120 Fragments arranged by size: 2120 1148 966 677 590 245 NcoI C'CATG_G Cuts at: 513 1065 2134 513 Size: 552 1069 4125 Fragments arranged by size: 4125 1069 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NsiI A_TGCA'T Cuts at: 2598 2598 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 2103 2131 2596 2749 2959 3104 4649 Size: 97 874 28 465 153 210 145 1545 Cuts at: 4649 1132 Size: 2229 Fragments arranged by size: 2229 1545 874 465 210 153 145 97 28 PasI CC'CwG_GG Cuts at: 1348 2167 1348 Size: 819 4927 PciI A'CATG_T Cuts at: 2745 4645 2745 Size: 1900 3846 PflMI CCAn_nnn'nTGG Cuts at: 1108 2167 1108 Size: 1059 4687 PfoI T'CCnGG_A Cuts at: 2538 2538 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2491 2491 Size: 5746 PshAI GACnn'nnGTC Cuts at: 1613 2679 1613 Size: 1066 4680 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PspXI vC'TCGA_Gb Cuts at: 2073 2073 Size: 5746 PstI C_TGCA'G Cuts at: 830 3098 830 Size: 2268 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 2572 2572 Size: 5746 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 2513 2561 3994 5706 2513 Size: 48 1433 1712 2553 Fragments arranged by size: 2553 1712 1433 48 SexAI A'CCwGG_T Cuts at: 3006 3006 Size: 5746 SfcI C'TryA_G Cuts at: 826 1045 3094 4229 4910 5101 826 Size: 219 2049 1135 681 191 1471 Fragments arranged by size: 2049 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 2917 3073 1872 Size: 1045 156 4545 Fragments arranged by size: 4545 1045 156 SmlI C'TyrA_G Cuts at: 820 1017 1943 2073 3850 4751 5013 820 Size: 197 926 130 1777 901 262 1553 Fragments arranged by size: 1777 1553 926 901 262 197 130 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 2103 3104 1132 Size: 971 1001 3774 Fragments arranged by size: 3774 1001 971 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 2071 1517 Size: 554 5192 StyI C'CwwG_G Cuts at: 513 1065 2066 2134 2358 2677 2802 3185 513 Size: 552 1001 68 224 319 125 383 3074 Fragments arranged by size: 3074 1001 552 383 319 224 125 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2427 3773 3790 3943 4128 4573 325 Size: 1255 847 1346 17 153 185 445 1498 Fragments arranged by size: 1498 1346 1255 847 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 2511 2559 Size: 274 80 33 51 1443 306 228 48 Cuts at: 2559 3992 5704 96 Size: 1433 1712 138 Fragments arranged by size: 1712 1443 1433 306 274 228 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5706 5738 5706 Size: 32 5714 Tth111I GACn'n_nGTC Cuts at: 1316 2375 1316 Size: 1059 4687 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2132 2299 2132 Size: 167 5579 XhoI C'TCGA_G Cuts at: 1943 2073 1943 Size: 130 5616 XmaI C'CCGG_G Cuts at: 1870 2915 3071 1870 Size: 1045 156 4545 Fragments arranged by size: 4545 1045 156 XmnI GAAnn'nnTTC Cuts at: 2867 3875 2867 Size: 1008 4738 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI