Restriction map for
pFN21AB4757
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII BalI BamHI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV FauI FbaI Fnu4HI
FseI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HpaII ItaI KasI
KpnI KspI MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacII
SalI Sau96I SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TseI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AvrII 205 CCTAGG C^CTAGG
BanI 1271 GGCACC G^G[CT][GA]CC
BfaI 206 CTAG C^TAG
BlnI 205 CCTAGG C^CTAGG
BsaBI 662 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 205 CCTAGG C^C[ACGT][ACGT]GG
BsgI 444 CTGCAC GTGCAG(16/14)
BsmBI 349 CGTCTC CGTCTC(1/5)
BsmI 823 GCATTC GAATGC(1/-1)
BspLU11I 550 ACATGT A^CATGT
BspMI 1342 ACCTGC ACCTGC(4/8)
BstBI 911 TTCGAA TT^CGAA
DpnII 668 GATC ^GATC
EcoNI 228 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 205 CCTAGG C^C[AT][AT]GG
EcoT22I 176 ATGCAT ATGCA^T
HindIII 318 AAGCTT A^AGCTT
MaeI 206 CTAG C^TAG
MaeII 348 ACGT A^CGT
MamI 662 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 668 GATC ^GATC
NdeII 668 GATC ^GATC
NsiI 176 ATGCAT ATGCA^T
NspV 911 TTCGAA TT^CGAA
SacI 498 GAGCTC GAGCT^C
SapI 902 GCTCTTC GCTCTTC(1/4)
Sau3AI 668 GATC ^GATC
SfcI 362 CTACAG C^T[GA][CT]AG
SfuI 911 TTCGAA TT^CGAA
SmlI 333 CTCAAG C^T[CT][GA]AG
StyI 205 CCTAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 744 CCGC CCGC(-3/-1)
1500 CCGC
AflIII 346 ACACGT A^C[GA][CT]GT
550 ACATGT
AspHI 498 GAGCTC G[AT]GC[AT]^C
1170 GAGCAC
BanII 498 GAGCTC G[GA]GC[CT]^C
1086 GAGCCC
BsaI 540 GAGACC GGTCTC(1/5)
1212 GAGACC
BsiHKAI 498 GAGCTC G[AT]GC[AT]^C
1170 GAGCAC
BsiYI 228 CCTTTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
996 CCAAACAAAGG
BslI 228 CCTTTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
996 CCAAACAAAGG
BsrDI 722 GCAATG GCAATG(2/0)
1310 GCAATG
BssKI 52 CCAGG ^CC[ACGT]GG
234 CCAGG
BstNI 52 CCAGG CC^[AT]GG
234 CCAGG
Cac8I 75 GCCTGC GC[ACGT]^[ACGT]GC
320 GCTTGC
EcoRII 52 CCAGG ^CC[AT]GG
234 CCAGG
MseI 1100 TTAA T^TAA
1331 TTAA
MvaI 52 CCAGG CC^[AT]GG
234 CCAGG
NlaIV 131 GGTTCC GG[ACGT]^[ACGT]CC
1271 GGCACC
NspI 178 GCATGT [GA]CATG^[CT]
550 ACATGT
PleI 106 GACTC GAGTC(4/5)
372 GAGTC
ScrFI 52 CCAGG CC^[ACGT]GG
234 CCAGG
TfiI 154 GATTC G^A[AT]TC
220 GAATC
Tru9I 1100 TTAA T^TAA
1331 TTAA
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BmyI
BpmI BsmAI Bsp1286I BsrI BstF5I
DdeI EarI EcoRI FokI HinfI
HphI Ksp632I MaeIII MboII MnlI
MslI NdeI NlaIII SfaNI TaqI
Tsp45I Tsp509I TspRI TthHB8I XmnI