(Circular) (Six-base) MAPSORT of: pFN21AB4757.seq Check: 1069 from: 1 to: 6337 pFN21AB4757 6337 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3356 3356 Size: 6337 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4147 278 Size: 53 83 186 936 2611 2468 Fragments arranged by size: 2611 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3680 1937 Size: 1743 4594 Acc65I G'GTAC_C Cuts at: 3658 3658 Size: 6337 AclI AA'CG_TT Cuts at: 1563 4464 4837 1563 Size: 2901 373 3063 Fragments arranged by size: 3063 2901 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2046 3571 4400 5784 6052 1632 Size: 414 1525 829 1384 268 1917 Fragments arranged by size: 1917 1525 1384 829 414 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6140 AflIII A'CryG_T Cuts at: 1203 2351 2555 5236 6213 1203 Size: 1148 204 2681 977 1327 Fragments arranged by size: 2681 1327 1148 977 204 AgeI A'CCGG_T Cuts at: 5168 5168 Size: 6337 AhdI GACnn_n'nnGTC Cuts at: 5066 5066 Size: 6337 AleI CACnn'nnGTG Cuts at: 1166 2624 2874 3462 3522 3546 3630 1166 Size: 1458 250 588 60 24 84 3873 Fragments arranged by size: 3873 1458 588 250 84 60 24 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6337 AlwNI CAG_nnn'CTG Cuts at: 4080 5652 4080 Size: 1572 4765 ApaLI G'TGCA_C Cuts at: 4394 5550 4394 Size: 1156 5181 ApoI r'AATT_y Cuts at: 1493 2613 2697 2740 2781 2865 2949 3033 3117 Size: 1120 84 43 41 84 84 84 84 Cuts at: 3117 3369 3537 3646 3872 4024 6133 1493 Size: 252 168 109 226 152 2109 1697 Fragments arranged by size: 2109 1697 1120 252 226 168 152 109 84 84 84 84 84 43 41 AseI AT'TA_AT Cuts at: 160 1032 4891 160 Size: 872 3859 1606 Fragments arranged by size: 3859 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6337 AvaI C'yCGr_G Cuts at: 1870 1943 3662 1870 Size: 73 1719 4545 Fragments arranged by size: 4545 1719 73 AvrII C'CTAG_G Cuts at: 2210 2210 Size: 6337 BamHI G'GATC_C Cuts at: 3667 4034 3667 Size: 367 5970 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3276 3658 5113 618 Size: 325 217 627 1489 382 1455 1842 Fragments arranged by size: 1842 1489 1455 627 382 325 217 BanII G_rGCy'C Cuts at: 1391 2507 3095 3656 1391 Size: 1116 588 561 4072 Fragments arranged by size: 4072 1116 588 561 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5366 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4528 4562 1903 Size: 34 2591 34 3678 Fragments arranged by size: 3678 2591 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4229 5439 4229 Size: 1210 5127 BclI T'GATC_A Cuts at: 1548 1548 Size: 6337 BfrBI ATG'CAT Cuts at: 2183 2183 Size: 6337 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4948 136 Size: 107 122 71 631 605 3276 1525 Fragments arranged by size: 3276 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6332 6332 Size: 6337 BlpI GC'TnA_GC Cuts at: 3754 3754 Size: 6337 Bme1580I G_kGCm'C Cuts at: 1790 4398 5554 1790 Size: 2608 1156 2573 Fragments arranged by size: 2608 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2354 1318 Size: 1036 5301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2967 4068 5026 Size: 416 53 83 399 24 1550 1101 958 Cuts at: 5026 442 Size: 1753 Fragments arranged by size: 1753 1550 1101 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6337 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3114 3114 Size: 6337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2559 2643 2727 2811 3147 3315 3399 Size: 869 451 84 84 84 336 168 84 Cuts at: 3399 4997 1239 Size: 1598 2579 Fragments arranged by size: 2579 1598 869 451 336 168 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2323 4462 5327 5625 2323 Size: 2139 865 298 3035 Fragments arranged by size: 3035 2139 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2539 3211 5000 882 Size: 617 1040 672 1789 2219 Fragments arranged by size: 2219 1789 1040 672 617 BsaAI yAC'GTr Cuts at: 493 1204 4128 493 Size: 711 2924 2702 Fragments arranged by size: 2924 2702 711 BsaBI GATnn'nnATC Cuts at: 2671 6331 2671 Size: 3660 2677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4144 4526 5161 275 Size: 53 83 186 936 2611 382 635 1451 Fragments arranged by size: 2611 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4770 5168 5442 5589 6000 4770 Size: 398 274 147 411 5107 Fragments arranged by size: 5107 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6112 Fragments arranged by size: 6112 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6337 BseYI C'CCAG_C Cuts at: 2061 5540 6036 2061 Size: 3479 496 2362 Fragments arranged by size: 3479 2362 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2434 1163 Size: 573 698 5066 Fragments arranged by size: 5066 698 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3787 4548 5576 1540 Size: 275 186 1786 761 1028 2301 Fragments arranged by size: 2301 1786 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2507 3179 4398 4483 5554 2507 Size: 672 1219 85 1071 3290 Fragments arranged by size: 3290 1219 1071 672 85 BsmI GAATG_Cn' Cuts at: 2828 3848 3941 2828 Size: 1020 93 5224 Fragments arranged by size: 5224 1020 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2360 2360 Size: 6337 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2507 3095 3179 3656 4398 4483 5554 Size: 399 717 588 84 477 742 85 1071 Cuts at: 5554 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 717 588 477 399 85 84 BspEI T'CCGG_A Cuts at: 6000 6000 Size: 6337 BspHI T'CATG_A Cuts at: 4226 5956 4226 Size: 1730 4607 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3356 3692 844 Size: 2512 336 3489 Fragments arranged by size: 3489 2512 336 BsrBI CCG'CTC Cuts at: 1394 1823 4224 1394 Size: 429 2401 3507 Fragments arranged by size: 3507 2401 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2734 3322 4832 5006 65 Size: 1183 1486 588 1510 174 1396 Fragments arranged by size: 1510 1486 1396 1183 588 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4981 5168 1700 Size: 110 3171 187 2869 Fragments arranged by size: 3171 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6337 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6337 BssSI C'ACGA_G Cuts at: 4397 5409 4397 Size: 1012 5325 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6337 BstBI TT'CG_AA Cuts at: 2917 5141 2917 Size: 2224 4113 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6337 BstYI r'GATC_y Cuts at: 1972 3667 4034 4419 4436 5888 5974 5986 6332 Size: 1695 367 385 17 1452 86 12 346 Cuts at: 6332 1972 Size: 1977 Fragments arranged by size: 1977 1695 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5785 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5598 Fragments arranged by size: 5598 683 56 BtsI GCAGTG_nn' Cuts at: 2654 2902 3238 3853 4647 4667 2654 Size: 248 336 615 794 20 4324 Fragments arranged by size: 4324 794 615 336 248 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6337 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2716 2751 2800 2835 3304 3339 529 Size: 35 2152 35 49 35 469 35 3527 Fragments arranged by size: 3527 2152 469 49 35 35 35 35 DraI TTT'AAA Cuts at: 3641 3641 Size: 6337 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5344 809 Size: 507 4028 1802 Fragments arranged by size: 4028 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3784 4673 6302 Size: 54 1425 50 275 18 1954 889 1629 Cuts at: 6302 8 Size: 43 Fragments arranged by size: 1954 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3784 1537 Size: 275 1972 4090 Fragments arranged by size: 4090 1972 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2022 2038 2367 2914 4267 1353 Size: 669 16 329 547 1353 3423 Fragments arranged by size: 3423 1353 669 547 329 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4924 5298 5444 387 Size: 1064 138 3335 374 146 1280 Fragments arranged by size: 3335 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2505 2505 Size: 6337 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2108 2559 2643 2727 2811 3147 Size: 393 414 62 451 84 84 84 336 Cuts at: 3147 3315 3399 3571 4400 4997 5784 6052 1239 Size: 168 84 172 829 597 787 268 1524 Fragments arranged by size: 1524 829 787 597 451 414 393 336 268 172 168 84 84 84 84 62 EcoNI CCTnn'n_nnAGG Cuts at: 2237 2237 Size: 6337 EcoRI G'AATT_C Cuts at: 2613 2697 2740 2781 2865 2949 3033 3117 3369 Size: 84 43 41 84 84 84 84 252 Cuts at: 3369 3537 3646 4024 2613 Size: 168 109 378 4926 Fragments arranged by size: 4926 378 252 168 109 84 84 84 84 84 43 41 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2067 2067 Size: 6337 FspI TGC'GCA Cuts at: 4843 4843 Size: 6337 HaeII r_GCGC'y Cuts at: 1678 5484 1678 Size: 3806 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2368 2400 4978 5010 5052 Size: 32 135 32 796 32 2578 32 42 Cuts at: 5052 5084 1373 Size: 32 2626 Fragments arranged by size: 2626 2578 796 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3681 3927 669 Size: 988 281 1743 246 3079 Fragments arranged by size: 3079 1743 988 281 246 HindIII A'AGCT_T Cuts at: 748 2323 748 Size: 1575 4762 HpaI GTT'AAC Cuts at: 3927 3927 Size: 6337 KpnI G_GTAC'C Cuts at: 3662 3662 Size: 6337 MfeI C'AATT_G Cuts at: 3936 3936 Size: 6337 MluI A'CGCG_T Cuts at: 6213 6213 Size: 6337 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2465 5451 5635 1305 Size: 1160 2986 184 2007 Fragments arranged by size: 2986 2007 1160 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4515 Fragments arranged by size: 4515 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2624 2638 2665 2874 2976 3142 3462 Size: 648 1458 14 27 209 102 166 320 Cuts at: 3462 3522 3546 3630 4295 4654 4813 518 Size: 60 24 84 665 359 159 2042 Fragments arranged by size: 2042 1458 665 648 359 320 209 166 159 102 84 60 27 24 14 MspA1I CmG'CkG Cuts at: 1606 3753 4430 5578 5823 1606 Size: 2147 677 1148 245 2120 Fragments arranged by size: 2147 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5785 NdeI CA'TA_TG Cuts at: 387 2635 2891 3363 387 Size: 2248 256 472 3361 Fragments arranged by size: 3361 2248 472 256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6337 NotI GC'GGCC_GC Cuts at: 3784 3784 Size: 6337 NsiI A_TGCA'T Cuts at: 2185 2185 Size: 6337 NspI r_CATG'y Cuts at: 1132 1229 2187 2559 3695 5240 1132 Size: 97 958 372 1136 1545 2229 Fragments arranged by size: 2229 1545 1136 958 372 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6337 PciI A'CATG_T Cuts at: 2555 5236 2555 Size: 2681 3656 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6337 PfoI T'CCnGG_A Cuts at: 2238 2238 Size: 6337 PmeI GTTT'AAAC Cuts at: 3641 3641 Size: 6337 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4406 4438 5919 5951 4406 Size: 32 1481 32 4792 Fragments arranged by size: 4792 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6337 PsiI TTA'TAA Cuts at: 2721 2805 3907 2721 Size: 84 1102 5151 Fragments arranged by size: 5151 1102 84 PstI C_TGCA'G Cuts at: 830 3689 830 Size: 2859 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6337 SacI G_AGCT'C Cuts at: 2507 2507 Size: 6337 SalI G'TCGA_C Cuts at: 1936 3679 1936 Size: 1743 4594 SapI GCTCTTCn'nnn_ Cuts at: 2914 2914 Size: 6337 SbfI CC_TGCA'GG Cuts at: 3689 3689 Size: 6337 ScaI AGT'ACT Cuts at: 4585 6297 4585 Size: 1712 4625 SfcI C'TryA_G Cuts at: 826 1045 2367 3685 4820 5501 5692 826 Size: 219 1322 1318 1135 681 191 1471 Fragments arranged by size: 1471 1322 1318 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6337 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6337 SmaI CCC'GGG Cuts at: 1872 3664 1872 Size: 1792 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2338 4441 5342 5604 820 Size: 197 926 395 2103 901 262 1553 Fragments arranged by size: 2103 1553 926 901 395 262 197 SnaBI TAC'GTA Cuts at: 493 4128 493 Size: 3635 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6337 SphI G_CATG'C Cuts at: 1132 3695 1132 Size: 2563 3774 SspI AAT'ATT Cuts at: 5 52 4261 5 Size: 47 4209 2081 Fragments arranged by size: 4209 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6337 StyI C'CwwG_G Cuts at: 513 1065 2210 3776 513 Size: 552 1145 1566 3074 Fragments arranged by size: 3074 1566 1145 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4364 4381 4534 4719 5164 325 Size: 1255 2784 17 153 185 445 1498 Fragments arranged by size: 2784 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4583 6295 96 Size: 274 80 33 51 1443 2606 1712 138 Fragments arranged by size: 2606 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6297 6329 6297 Size: 32 6305 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6337 XbaI T'CTAG_A Cuts at: 3673 3673 Size: 6337 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6337 XmaI C'CCGG_G Cuts at: 1870 3662 1870 Size: 1792 4545 XmnI GAAnn'nnTTC Cuts at: 2744 2828 2996 3500 4466 2744 Size: 84 168 504 966 4615 Fragments arranged by size: 4615 966 504 168 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4145 276 Size: 53 83 186 936 2611 2468 Fragments arranged by size: 2611 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI