(Circular) (Six-base) MAPSORT of: pFN21AB4745.seq Check: 5673 from: 1 to: 5824 pFN21AB4745 5824 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2844 3167 1937 Size: 907 323 4594 Fragments arranged by size: 4594 907 323 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2285 3887 5271 5539 1632 Size: 653 1602 1384 268 1917 Fragments arranged by size: 1917 1602 1384 653 268 AfeI AGC'GCT Cuts at: 2426 2426 Size: 5824 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 4723 5700 1203 Size: 3520 977 1327 Fragments arranged by size: 3520 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 2702 1166 Size: 1536 4288 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5824 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2168 2200 2168 Size: 32 5792 AlwNI CAG_nnn'CTG Cuts at: 2101 2614 3567 5139 2101 Size: 513 953 1572 2786 Fragments arranged by size: 2786 1572 953 513 ApaI G_GGCC'C Cuts at: 2346 2346 Size: 5824 ApaLI G'TGCA_C Cuts at: 2976 3881 5037 2976 Size: 905 1156 3763 Fragments arranged by size: 3763 1156 905 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2350 3149 1870 Size: 73 407 799 4545 Fragments arranged by size: 4545 799 407 73 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3145 4600 618 Size: 325 217 627 1358 1455 1842 Fragments arranged by size: 1842 1455 1358 627 325 217 BanII G_rGCy'C Cuts at: 1391 2038 2346 2556 3143 1391 Size: 647 308 210 587 4072 Fragments arranged by size: 4072 647 587 308 210 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4853 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4015 4049 1903 Size: 34 2078 34 3678 Fragments arranged by size: 3678 2078 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4435 136 Size: 107 122 71 631 605 2763 1525 Fragments arranged by size: 2763 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 2346 2980 3885 5041 1790 Size: 556 634 905 1156 2573 Fragments arranged by size: 2573 1156 905 634 556 BmgBI CAC'GTC Cuts at: 1318 2869 1318 Size: 1551 4273 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2349 3555 4513 Size: 416 53 83 399 24 932 1206 958 Cuts at: 4513 442 Size: 1753 Fragments arranged by size: 1753 1206 958 932 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3132 3132 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2484 2660 2804 2942 4484 1239 Size: 1245 176 144 138 1542 2579 Fragments arranged by size: 2579 1542 1245 176 144 138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3949 4814 5112 3949 Size: 865 298 4661 Fragments arranged by size: 4661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2023 4487 882 Size: 617 524 2464 2219 Fragments arranged by size: 2464 2219 617 524 BsaAI yAC'GTr Cuts at: 493 1204 2044 3615 493 Size: 711 840 1571 2702 Fragments arranged by size: 2702 1571 840 711 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2876 3631 4013 4648 Size: 53 83 186 936 1343 755 382 635 Cuts at: 4648 275 Size: 1451 Fragments arranged by size: 1451 1343 936 755 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2595 4257 4655 4929 5076 5487 2595 Size: 1662 398 274 147 411 2932 Fragments arranged by size: 2932 1662 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2168 2198 1178 Size: 30 165 30 765 30 4804 Fragments arranged by size: 4804 765 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2466 2582 2657 1186 Size: 1280 116 75 4353 Fragments arranged by size: 4353 1280 116 75 BseYI C'CCAG_C Cuts at: 5027 5523 5027 Size: 496 5328 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2024 2627 2702 2713 1163 Size: 573 288 603 75 11 4274 Fragments arranged by size: 4274 603 573 288 75 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2038 2556 2800 2817 2980 3885 3970 5041 2038 Size: 518 244 17 163 905 85 1071 2821 Fragments arranged by size: 2821 1071 905 518 244 163 85 17 BsmI GAATG_Cn' Cuts at: 3335 3428 3335 Size: 93 5731 BsmBI CGTCTCn'nnnn_ Cuts at: 3091 3091 Size: 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2346 2556 2800 2817 2980 3143 Size: 399 248 308 210 244 17 163 163 Cuts at: 3143 3885 3970 5041 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 308 248 244 210 163 163 85 17 BspEI T'CCGG_A Cuts at: 2595 5487 2595 Size: 2892 2932 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2407 3179 844 Size: 1563 772 3489 Fragments arranged by size: 3489 1563 772 BsrBI CCG'CTC Cuts at: 1394 1823 2626 3711 1394 Size: 429 803 1085 3507 Fragments arranged by size: 3507 1085 803 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4319 4493 65 Size: 1183 3071 174 1396 Fragments arranged by size: 3071 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2732 2897 3045 4468 4655 1700 Size: 110 922 165 148 1423 187 2869 Fragments arranged by size: 2869 1423 922 187 165 148 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 2614 794 Size: 1820 4004 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstXI CCAn_nnnn'nTGG Cuts at: 2716 3029 2716 Size: 313 5511 BstYI r'GATC_y Cuts at: 1972 2586 3154 3521 3906 3923 5375 5461 5473 Size: 614 568 367 385 17 1452 86 12 Cuts at: 5473 5819 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 614 568 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2172 2328 2919 2997 513 Size: 552 939 168 156 591 78 3340 Fragments arranged by size: 3340 939 591 552 168 156 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2899 487 Size: 683 56 1673 3412 Fragments arranged by size: 3412 1673 683 56 BtsI GCAGTG_nn' Cuts at: 2797 3340 4134 4154 2797 Size: 543 794 20 4467 Fragments arranged by size: 4467 794 543 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DraIII CAC_nnn'GTG Cuts at: 2047 2152 2047 Size: 105 5719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3083 4831 809 Size: 507 1767 1748 1802 Fragments arranged by size: 1802 1767 1748 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2325 2706 2922 Size: 54 1425 50 275 18 495 381 216 Cuts at: 2922 3271 4160 5789 8 Size: 349 889 1629 43 Fragments arranged by size: 1629 1425 889 495 381 349 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3754 1353 Size: 2401 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2743 4411 4785 4931 387 Size: 1064 138 1154 1668 374 146 1280 Fragments arranged by size: 1668 1280 1154 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2036 2554 2036 Size: 518 5306 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2484 2660 2804 2942 3887 4484 Size: 393 653 199 176 144 138 945 597 Cuts at: 4484 5271 5539 1239 Size: 787 268 1524 Fragments arranged by size: 1524 945 787 653 597 393 268 199 176 144 138 EcoNI CCTnn'n_nnAGG Cuts at: 2597 2597 Size: 5824 EcoO109I rG'GnC_Cy Cuts at: 2010 2098 2299 2343 3076 2010 Size: 88 201 44 733 4758 Fragments arranged by size: 4758 733 201 88 44 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2155 2248 2155 Size: 93 5731 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2428 4971 1678 Size: 750 2543 2531 Fragments arranged by size: 2543 2531 750 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2658 2690 2875 2907 4465 Size: 32 135 32 1086 32 185 32 1558 Cuts at: 4465 4497 4539 4571 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1558 1086 185 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2935 3168 3414 669 Size: 988 281 997 233 246 3079 Fragments arranged by size: 3079 997 988 281 246 233 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 2708 2924 10 Size: 54 1425 343 495 381 216 2910 Fragments arranged by size: 2910 1425 495 381 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2148 2441 2678 2702 2918 3782 Size: 648 954 28 293 237 24 216 864 Cuts at: 3782 4141 4300 518 Size: 359 159 2042 Fragments arranged by size: 2042 954 864 648 359 293 237 216 159 28 24 MspA1I CmG'CkG Cuts at: 1606 2092 2611 2950 3240 3917 5065 5310 1606 Size: 486 519 339 290 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 519 486 339 290 245 NaeI GCC'GGC Cuts at: 2899 3047 2899 Size: 148 5676 NcoI C'CATG_G Cuts at: 513 1065 2004 2997 513 Size: 552 939 993 3340 Fragments arranged by size: 3340 993 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NgoMIV G'CCGG_C Cuts at: 2897 3045 2897 Size: 148 5676 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 3182 4727 1132 Size: 97 1953 1545 2229 Fragments arranged by size: 2229 1953 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5824 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 2152 1108 Size: 1044 4780 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PmlI CAC'GTG Cuts at: 2044 2044 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2168 2200 3893 3925 5406 5438 2168 Size: 32 1693 32 1481 32 2554 Fragments arranged by size: 2554 1693 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2010 3076 2010 Size: 1066 4758 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2342 2342 Size: 5824 PstI C_TGCA'G Cuts at: 830 2097 2289 3176 830 Size: 1267 192 887 3478 Fragments arranged by size: 3478 1267 887 192 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2092 2611 2092 Size: 519 5305 SacI G_AGCT'C Cuts at: 2038 2556 2038 Size: 518 5306 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SanDI GG'GwC_CC Cuts at: 2010 2010 Size: 5824 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SexAI A'CCwGG_T Cuts at: 2715 2715 Size: 5824 SfcI C'TryA_G Cuts at: 826 1045 2093 2285 3172 4307 4988 5179 826 Size: 219 1048 192 887 1135 681 191 1471 Fragments arranged by size: 1471 1135 1048 887 681 219 192 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 2352 3151 1872 Size: 480 799 4545 Fragments arranged by size: 4545 799 480 SmlI C'TyrA_G Cuts at: 820 1017 1943 3928 4829 5091 820 Size: 197 926 1985 901 262 1553 Fragments arranged by size: 1985 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 2396 2963 1517 Size: 879 567 4378 Fragments arranged by size: 4378 879 567 StyI C'CwwG_G Cuts at: 513 1065 2004 2997 3263 513 Size: 552 939 993 266 3074 Fragments arranged by size: 3074 993 939 552 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2045 3851 3868 4021 4206 4651 325 Size: 1255 465 1806 17 153 185 445 1498 Fragments arranged by size: 1806 1498 1255 465 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2670 4070 5782 Size: 274 80 33 51 1443 693 1400 1712 Cuts at: 5782 96 Size: 138 Fragments arranged by size: 1712 1443 1400 693 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2970 3002 5784 5816 2970 Size: 32 2782 32 2978 Fragments arranged by size: 2978 2782 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2917 2917 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 2350 3149 1870 Size: 480 799 4545 Fragments arranged by size: 4545 799 480 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI