(Circular) (Six-base) MAPSORT of: pFN21AB4737.seq Check: 7691 from: 1 to: 8803 pFN21AB4737 8803 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2381 4874 2381 Size: 2493 6310 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6613 278 Size: 53 83 186 936 5077 2468 Fragments arranged by size: 5077 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4654 5583 6146 1937 Size: 2717 929 563 4594 Fragments arranged by size: 4594 2717 929 563 Acc65I G'GTAC_C Cuts at: 6124 6124 Size: 8803 AclI AA'CG_TT Cuts at: 1563 6930 7303 1563 Size: 5367 373 3063 Fragments arranged by size: 5367 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5441 5481 6866 8250 8518 1632 Size: 3809 40 1385 1384 268 1917 Fragments arranged by size: 3809 1917 1385 1384 268 40 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8606 AflIII A'CryG_T Cuts at: 1203 2674 5677 7702 8679 1203 Size: 1471 3003 2025 977 1327 Fragments arranged by size: 3003 2025 1471 1327 977 AgeI A'CCGG_T Cuts at: 7634 7634 Size: 8803 AhdI GACnn_n'nnGTC Cuts at: 7532 7532 Size: 8803 AleI CACnn'nnGTG Cuts at: 1166 5118 1166 Size: 3952 4851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8803 AlwNI CAG_nnn'CTG Cuts at: 3575 3673 3854 6546 8118 3575 Size: 98 181 2692 1572 4260 Fragments arranged by size: 4260 2692 1572 181 98 ApaI G_GGCC'C Cuts at: 2105 3108 3260 4720 5052 5168 5623 2105 Size: 1003 152 1460 332 116 455 5285 Fragments arranged by size: 5285 1460 1003 455 332 152 116 ApaLI G'TGCA_C Cuts at: 4887 6860 8016 4887 Size: 1973 1156 5674 Fragments arranged by size: 5674 1973 1156 ApoI r'AATT_y Cuts at: 1493 6068 6112 6338 6490 8599 1493 Size: 4575 44 226 152 2109 1697 Fragments arranged by size: 4575 2109 1697 226 152 44 AseI AT'TA_AT Cuts at: 160 1032 7357 160 Size: 872 6325 1606 Fragments arranged by size: 6325 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8803 AvaI C'yCGr_G Cuts at: 1870 1943 3558 4125 4636 6128 1870 Size: 73 1615 567 511 1492 4545 Fragments arranged by size: 4545 1615 1492 567 511 73 AvrII C'CTAG_G Cuts at: 5159 5159 Size: 8803 BamHI G'GATC_C Cuts at: 6133 6500 6133 Size: 367 8436 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2609 2681 2699 2780 2924 Size: 325 217 627 822 72 18 81 144 Cuts at: 2924 3023 4049 6124 7579 618 Size: 99 1026 2075 1455 1842 Fragments arranged by size: 2075 1842 1455 1026 822 627 325 217 144 99 81 72 18 BanII G_rGCy'C Cuts at: 1391 2105 2326 2650 2965 3093 3108 3260 4720 Size: 714 221 324 315 128 15 152 1460 Cuts at: 4720 5037 5052 5168 5623 5663 5888 6122 1391 Size: 317 15 116 455 40 225 234 4072 Fragments arranged by size: 4072 1460 714 455 324 317 315 234 225 221 152 128 116 40 15 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2855 3215 3350 4592 5051 928 Size: 971 956 360 135 1242 459 4680 Fragments arranged by size: 4680 1242 971 956 459 360 135 BbvCI CC'TCA_GC Cuts at: 5910 5910 Size: 8803 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6994 7028 1903 Size: 34 50 34 4973 34 3678 Fragments arranged by size: 4973 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2014 5624 6695 7905 2014 Size: 3610 1071 1210 2912 Fragments arranged by size: 3610 2912 1210 1071 BclI T'GATC_A Cuts at: 1548 5122 1548 Size: 3574 5229 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2154 2418 2769 Size: 107 122 71 631 605 482 264 351 Cuts at: 2769 2877 2949 3417 3444 5010 5271 7414 136 Size: 108 72 468 27 1566 261 2143 1525 Fragments arranged by size: 2143 1566 1525 631 605 482 468 351 264 261 122 108 107 72 71 27 BglII A'GATC_T Cuts at: 8798 8798 Size: 8803 BlpI GC'TnA_GC Cuts at: 2009 5374 6220 2009 Size: 3365 846 4592 Fragments arranged by size: 4592 3365 846 Bme1580I G_kGCm'C Cuts at: 1790 2105 2614 2686 2704 2785 3108 3260 3899 Size: 315 509 72 18 81 323 152 639 Cuts at: 3899 4054 4720 4891 5052 5168 5623 6864 8020 Size: 155 666 171 161 116 455 1241 1156 Cuts at: 8020 1790 Size: 2573 Fragments arranged by size: 2573 1241 1156 666 639 509 455 323 315 171 161 155 152 116 81 72 18 BmgBI CAC'GTC Cuts at: 1318 2675 5680 1318 Size: 1357 3005 4441 Fragments arranged by size: 4441 3005 1357 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3120 6083 6534 Size: 416 53 83 399 24 1703 2963 451 Cuts at: 6534 7492 442 Size: 958 1753 Fragments arranged by size: 2963 1753 1703 958 451 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8803 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3112 3112 Size: 8803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2340 2616 2751 2925 3165 3249 3312 3474 Size: 1101 276 135 174 240 84 63 162 Cuts at: 3474 3552 3642 3978 4086 4518 4569 4572 4770 Size: 78 90 336 108 432 51 3 198 Cuts at: 4770 5289 7463 1239 Size: 519 2174 2579 Fragments arranged by size: 2579 2174 1101 519 432 336 276 240 198 174 162 135 108 90 84 78 63 51 3 Bpu10I CC'TnA_GC Cuts at: 3594 5910 3594 Size: 2316 6487 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2319 5521 6928 7793 8091 2319 Size: 3202 1407 865 298 3031 Fragments arranged by size: 3202 3031 1407 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2225 3344 3731 3749 3992 4631 5438 Size: 617 726 1119 387 18 243 639 807 Cuts at: 5438 7466 882 Size: 2028 2219 Fragments arranged by size: 2219 2028 1119 807 726 639 617 387 243 18 BsaAI yAC'GTr Cuts at: 493 1204 2126 6594 493 Size: 711 922 4468 2702 Fragments arranged by size: 4468 2702 922 711 BsaBI GATnn'nnATC Cuts at: 8797 8797 Size: 8803 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3249 6610 6992 7627 Size: 53 83 186 936 1716 3361 382 635 Cuts at: 7627 275 Size: 1451 Fragments arranged by size: 3361 1716 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7236 7634 7908 8055 8466 7236 Size: 398 274 147 411 7573 Fragments arranged by size: 7573 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2170 2200 5515 5545 1178 Size: 30 165 30 767 30 3315 30 4436 Fragments arranged by size: 4436 3315 767 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2487 3477 4242 5861 1186 Size: 1301 990 765 1619 4128 Fragments arranged by size: 4128 1619 1301 990 765 BseYI C'CCAG_C Cuts at: 2104 2692 2957 3101 4091 4294 4442 5479 5638 Size: 588 265 144 990 203 148 1037 159 Cuts at: 5638 8006 8502 2104 Size: 2368 496 2405 Fragments arranged by size: 2405 2368 1037 990 588 496 265 203 159 148 144 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2334 4530 1163 Size: 573 598 2196 5436 Fragments arranged by size: 5436 2196 598 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6253 7014 8042 1540 Size: 275 186 4252 761 1028 2301 Fragments arranged by size: 4252 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2398 2497 2587 3559 3568 3595 4027 4036 4612 Size: 99 90 972 9 27 432 9 576 Cuts at: 4612 4765 4891 5134 5663 6864 6949 8020 2398 Size: 153 126 243 529 1201 85 1071 3181 Fragments arranged by size: 3181 1201 1071 972 576 529 432 243 153 126 99 90 85 27 9 9 BsmI GAATG_Cn' Cuts at: 5013 5238 6314 6407 5013 Size: 225 1076 93 7409 Fragments arranged by size: 7409 1076 225 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 2326 2398 2497 2587 2614 2650 Size: 399 315 221 72 99 90 27 36 Cuts at: 2650 2686 2704 2785 2965 3093 3108 3260 3559 Size: 36 18 81 180 128 15 152 299 Cuts at: 3559 3568 3595 3899 4027 4036 4054 4612 4720 Size: 9 27 304 128 9 18 558 108 Cuts at: 4720 4765 4891 5037 5052 5134 5168 5623 5663 Size: 45 126 146 15 82 34 455 40 Cuts at: 5663 5888 6122 6864 6949 8020 1391 Size: 225 234 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 558 455 399 315 304 299 234 225 221 180 152 146 128 128 126 108 99 90 85 82 81 72 45 40 36 36 34 27 27 18 18 15 15 9 9 BspEI T'CCGG_A Cuts at: 8466 8466 Size: 8803 BspHI T'CATG_A Cuts at: 6692 8422 6692 Size: 1730 7073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2359 2381 4874 5239 5913 6158 844 Size: 1515 22 2493 365 674 245 3489 Fragments arranged by size: 3489 2493 1515 674 365 245 22 BsrBI CCG'CTC Cuts at: 1394 1823 3075 6690 1394 Size: 429 1252 3615 3507 Fragments arranged by size: 3615 3507 1252 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4698 5829 7298 7472 65 Size: 1183 3450 1131 1469 174 1396 Fragments arranged by size: 3450 1469 1396 1183 1131 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2840 3011 3173 3803 4703 7447 7634 Size: 110 1030 171 162 630 900 2744 187 Cuts at: 7634 1700 Size: 2869 Fragments arranged by size: 2869 2744 1030 900 630 187 171 162 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8803 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8803 BssSI C'ACGA_G Cuts at: 2397 2496 3216 4026 4467 6863 7875 2397 Size: 99 720 810 441 2396 1012 3325 Fragments arranged by size: 3325 2396 1012 810 720 441 99 BstAPI GCAn_nnn'nTGC Cuts at: 794 5095 794 Size: 4301 4502 BstBI TT'CG_AA Cuts at: 7607 7607 Size: 8803 BstEII G'GTnAC_C Cuts at: 1184 2438 4004 4076 4472 4850 5111 5255 1184 Size: 1254 1566 72 396 378 261 144 4732 Fragments arranged by size: 4732 1566 1254 396 378 261 144 72 BstXI CCAn_nnnn'nTGG Cuts at: 5752 5752 Size: 8803 BstYI r'GATC_y Cuts at: 1972 3167 6133 6500 6885 6902 8354 8440 8452 Size: 1195 2966 367 385 17 1452 86 12 Cuts at: 8452 8798 1972 Size: 346 1977 Fragments arranged by size: 2966 1977 1452 1195 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 5584 5584 Size: 8803 Bsu36I CC'TnA_GG Cuts at: 3685 4242 4593 5250 3685 Size: 557 351 657 7238 Fragments arranged by size: 7238 657 557 351 BtgI C'CryG_G Cuts at: 513 1065 2650 2704 2730 2848 2983 3730 4720 Size: 552 1585 54 26 118 135 747 990 Cuts at: 4720 4908 4936 5116 5142 513 Size: 188 28 180 26 4174 Fragments arranged by size: 4174 1585 990 747 552 188 180 135 118 54 28 26 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4833 487 Size: 683 56 3607 4457 Fragments arranged by size: 4457 3607 683 56 BtsI GCAGTG_nn' Cuts at: 2716 3897 5533 5815 6319 7113 7133 2716 Size: 1181 1636 282 504 794 20 4386 Fragments arranged by size: 4386 1636 1181 794 504 282 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8768 DraI TTT'AAA Cuts at: 6107 6107 Size: 8803 DraIII CAC_nnn'GTG Cuts at: 2312 2492 3617 2312 Size: 180 1125 7498 Fragments arranged by size: 7498 1125 180 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2553 3354 4389 7810 809 Size: 507 1237 801 1035 3421 1802 Fragments arranged by size: 3421 1802 1237 1035 801 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4489 5784 6250 Size: 54 1425 50 275 18 2659 1295 466 Cuts at: 6250 7139 8768 8 Size: 889 1629 43 Fragments arranged by size: 2659 1629 1425 1295 889 466 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6250 1537 Size: 275 4438 4090 Fragments arranged by size: 4438 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 6733 1353 Size: 5380 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7390 7764 7910 387 Size: 1064 138 5801 374 146 1280 Fragments arranged by size: 5801 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5661 5661 Size: 8803 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2340 2616 2751 2925 3165 3249 3312 Size: 393 708 276 135 174 240 84 63 Cuts at: 3312 3474 3552 3642 3978 4086 4518 4569 4572 Size: 162 78 90 336 108 432 51 3 Cuts at: 4572 4770 5289 5441 5481 6866 7463 8250 8518 Size: 198 519 152 40 1385 597 787 268 Cuts at: 8518 1239 Size: 1524 Fragments arranged by size: 1524 1385 787 708 597 519 432 393 336 276 268 240 198 174 162 152 135 108 90 84 78 63 51 40 3 EcoNI CCTnn'n_nnAGG Cuts at: 5553 5864 5553 Size: 311 8492 EcoO109I rG'GnC_Cy Cuts at: 2101 2147 2183 2294 2330 2348 2726 2735 3105 Size: 46 36 111 36 18 378 9 370 Cuts at: 3105 3113 3149 3185 3257 3302 3347 3401 3500 Size: 8 36 36 72 45 45 54 99 Cuts at: 3500 3572 3599 3644 3689 3833 3851 4238 4274 Size: 72 27 45 45 144 18 387 36 Cuts at: 4274 4346 4400 4544 4589 4716 4913 4994 5048 Size: 72 54 144 45 127 197 81 54 Cuts at: 5048 5147 5164 5165 5246 5264 2101 Size: 99 17 1 81 18 5640 Fragments arranged by size: 5640 387 378 370 197 144 144 127 111 99 99 81 81 72 72 72 54 54 54 46 45 45 45 45 45 36 36 36 36 36 27 18 18 18 17 9 8 1 EcoRI G'AATT_C Cuts at: 6068 6112 6490 6068 Size: 44 378 8381 Fragments arranged by size: 8381 378 44 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2879 4472 2879 Size: 1593 7210 FspI TGC'GCA Cuts at: 7309 7309 Size: 8803 HaeII r_GCGC'y Cuts at: 1678 7950 1678 Size: 6272 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3190 3222 3234 3266 5682 Size: 32 135 32 1618 32 12 32 2416 Cuts at: 5682 5714 5939 5971 7444 7476 7518 7550 1373 Size: 32 225 32 1473 32 42 32 2626 Fragments arranged by size: 2626 2416 1618 1473 225 135 42 32 32 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 669 1657 1938 4205 4756 4826 6147 6393 669 Size: 988 281 2267 551 70 1321 246 3079 Fragments arranged by size: 3079 2267 1321 988 551 281 246 70 HindIII A'AGCT_T Cuts at: 748 748 Size: 8803 HpaI GTT'AAC Cuts at: 6393 6393 Size: 8803 KpnI G_GTAC'C Cuts at: 6128 6128 Size: 8803 MfeI C'AATT_G Cuts at: 3441 6402 3441 Size: 2961 5842 MluI A'CGCG_T Cuts at: 8679 8679 Size: 8803 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2242 2461 2866 4261 5206 7917 8101 1305 Size: 937 219 405 1395 945 2711 184 2007 Fragments arranged by size: 2711 2007 1395 945 937 405 219 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5786 10 Size: 54 1425 343 3954 3027 Fragments arranged by size: 3954 3027 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5118 6761 7120 7279 518 Size: 648 3952 1643 359 159 2042 Fragments arranged by size: 3952 2042 1643 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2318 2408 2696 2985 3056 3389 3452 3650 Size: 712 90 288 289 71 333 63 198 Cuts at: 3650 3695 4298 6219 6896 8044 8289 1606 Size: 45 603 1921 677 1148 245 2120 Fragments arranged by size: 2120 1921 1148 712 677 603 333 289 288 245 198 90 71 63 45 NaeI GCC'GGC Cuts at: 3175 4705 3175 Size: 1530 7273 NcoI C'CATG_G Cuts at: 513 1065 3730 5116 513 Size: 552 2665 1386 4200 Fragments arranged by size: 4200 2665 1386 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8803 NgoMIV G'CCGG_C Cuts at: 3173 4703 3173 Size: 1530 7273 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8803 NotI GC'GGCC_GC Cuts at: 6250 6250 Size: 8803 NruI TCG'CGA Cuts at: 4818 4818 Size: 8803 NspI r_CATG'y Cuts at: 1132 1229 2070 5493 6161 7706 1132 Size: 97 841 3423 668 1545 2229 Fragments arranged by size: 3423 2229 1545 841 668 97 PasI CC'CwG_GG Cuts at: 1348 3326 3848 4793 1348 Size: 1978 522 945 5358 Fragments arranged by size: 5358 1978 945 522 PciI A'CATG_T Cuts at: 7702 7702 Size: 8803 PflMI CCAn_nnn'nTGG Cuts at: 1108 5649 1108 Size: 4541 4262 PfoI T'CCnGG_A Cuts at: 2515 2902 3181 3226 3604 4747 5296 2515 Size: 387 279 45 378 1143 549 6022 Fragments arranged by size: 6022 1143 549 387 378 279 45 PmeI GTTT'AAAC Cuts at: 6107 6107 Size: 8803 PmlI CAC'GTG Cuts at: 2126 2126 Size: 8803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3972 4004 6872 6904 8385 8417 3972 Size: 32 2868 32 1481 32 4358 Fragments arranged by size: 4358 2868 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2294 2348 2726 2735 3113 3185 3302 3347 3401 Size: 54 378 9 378 72 117 45 54 Cuts at: 3401 3572 3599 3644 3689 3833 4400 4544 4589 Size: 171 27 45 45 144 567 144 45 Cuts at: 4589 4913 4994 5147 2294 Size: 324 81 153 5950 Fragments arranged by size: 5950 567 378 378 324 171 153 144 144 117 81 72 54 54 45 45 45 45 27 9 PshAI GACnn'nnGTC Cuts at: 1613 4479 1613 Size: 2866 5937 PsiI TTA'TAA Cuts at: 6373 6373 Size: 8803 PspOMI G'GGCC_C Cuts at: 2101 3104 3256 4716 5048 5164 5619 2101 Size: 1003 152 1460 332 116 455 5285 Fragments arranged by size: 5285 1460 1003 455 332 152 116 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3196 3228 4638 4670 5795 5827 3196 Size: 32 1410 32 1125 32 6172 Fragments arranged by size: 6172 1410 1125 32 32 32 PstI C_TGCA'G Cuts at: 830 2392 4885 6155 830 Size: 1562 2493 1270 3478 Fragments arranged by size: 3478 2493 1562 1270 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8803 PvuII CAG'CTG Cuts at: 2318 2408 2696 3056 4298 2318 Size: 90 288 360 1242 6823 Fragments arranged by size: 6823 1242 360 288 90 SacI G_AGCT'C Cuts at: 5663 5663 Size: 8803 SacII CC_GC'GG Cuts at: 2986 2986 Size: 8803 SalI G'TCGA_C Cuts at: 1936 6145 1936 Size: 4209 4594 SanDI GG'GwC_CC Cuts at: 2726 3347 3644 4544 2726 Size: 621 297 900 6985 Fragments arranged by size: 6985 900 621 297 SbfI CC_TGCA'GG Cuts at: 2392 6155 2392 Size: 3763 5040 ScaI AGT'ACT Cuts at: 7051 8763 7051 Size: 1712 7091 SexAI A'CCwGG_T Cuts at: 2197 2308 2371 2461 2605 3874 3982 4207 4657 Size: 111 63 90 144 1269 108 225 450 Cuts at: 4657 4684 4891 2197 Size: 27 207 6109 Fragments arranged by size: 6109 1269 450 225 207 144 111 108 90 63 27 SfcI C'TryA_G Cuts at: 826 1045 2388 4044 4881 5353 5879 6151 7286 Size: 219 1343 1656 837 472 526 272 1135 Cuts at: 7286 7967 8158 826 Size: 681 191 1471 Fragments arranged by size: 1656 1471 1343 1135 837 681 526 472 272 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2877 3444 5271 2877 Size: 567 1827 6409 Fragments arranged by size: 6409 1827 567 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8803 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8803 SmaI CCC'GGG Cuts at: 1872 6130 1872 Size: 4258 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2334 4636 5500 6907 7808 8070 Size: 197 926 391 2302 864 1407 901 262 Cuts at: 8070 820 Size: 1553 Fragments arranged by size: 2302 1553 1407 926 901 864 391 262 197 SnaBI TAC'GTA Cuts at: 493 6594 493 Size: 6101 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8803 SphI G_CATG'C Cuts at: 1132 6161 1132 Size: 5029 3774 SspI AAT'ATT Cuts at: 5 52 4864 6727 5 Size: 47 4812 1863 2081 Fragments arranged by size: 4812 2081 1863 47 StuI AGG'CCT Cuts at: 1517 2871 3433 1517 Size: 1354 562 6887 Fragments arranged by size: 6887 1354 562 StyI C'CwwG_G Cuts at: 513 1065 2344 2793 2928 3109 3730 4477 5116 Size: 552 1279 449 135 181 621 747 639 Cuts at: 5116 5159 5260 5394 5554 5664 5745 6242 513 Size: 43 101 134 160 110 81 497 3074 Fragments arranged by size: 3074 1279 747 639 621 552 497 449 181 160 135 134 110 101 81 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4145 4667 5529 6830 6847 7000 7185 Size: 1255 2565 522 862 1301 17 153 185 Cuts at: 7185 7630 325 Size: 445 1498 Fragments arranged by size: 2565 1498 1301 1255 862 522 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7049 8761 96 Size: 274 80 33 51 1443 5072 1712 138 Fragments arranged by size: 5072 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2170 2202 3496 3528 3889 3921 4573 4605 5197 Size: 32 1294 32 361 32 652 32 592 Cuts at: 5197 5229 8763 8795 2170 Size: 32 3534 32 2178 Fragments arranged by size: 3534 2178 1294 652 592 361 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8803 XbaI T'CTAG_A Cuts at: 5468 6139 5468 Size: 671 8132 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2351 3116 3674 3737 4484 5267 5646 2351 Size: 765 558 63 747 783 379 5508 Fragments arranged by size: 5508 783 765 747 558 379 63 XhoI C'TCGA_G Cuts at: 1943 4636 1943 Size: 2693 6110 XmaI C'CCGG_G Cuts at: 1870 6128 1870 Size: 4258 4545 XmnI GAAnn'nnTTC Cuts at: 4455 6932 4455 Size: 2477 6326 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6611 276 Size: 53 83 186 936 5077 2468 Fragments arranged by size: 5077 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI EcoRV FseI FspAI KasI NarI NsiI PacI PspXI RsrII SapI SfoI SrfI SwaI