(Circular) (Six-base) MAPSORT of: pFN21AB4702.seq Check: 1799 from: 1 to: 6427 pFN21AB4702 6427 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4237 278 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2043 3770 1937 Size: 106 1727 4594 Fragments arranged by size: 4594 1727 106 Acc65I G'GTAC_C Cuts at: 3748 3748 Size: 6427 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4490 5874 6142 1632 Size: 2858 1384 268 1917 Fragments arranged by size: 2858 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6230 AflIII A'CryG_T Cuts at: 1203 2830 5326 6303 1203 Size: 1627 2496 977 1327 Fragments arranged by size: 2496 1627 1327 977 AgeI A'CCGG_T Cuts at: 5258 5258 Size: 6427 AhdI GACnn_n'nnGTC Cuts at: 5156 5156 Size: 6427 AleI CACnn'nnGTG Cuts at: 1166 2605 1166 Size: 1439 4988 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2233 1184 Size: 1049 5378 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2121 2153 2121 Size: 32 6395 AlwNI CAG_nnn'CTG Cuts at: 2843 4170 5742 2843 Size: 1327 1572 3528 Fragments arranged by size: 3528 1572 1327 ApaLI G'TGCA_C Cuts at: 4484 5640 4484 Size: 1156 5271 ApoI r'AATT_y Cuts at: 1493 2278 3216 3287 3350 3736 3962 4114 6223 Size: 785 938 71 63 386 226 152 2109 Cuts at: 6223 1493 Size: 1697 Fragments arranged by size: 2109 1697 938 785 386 226 152 71 63 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 3752 1870 Size: 73 1809 4545 Fragments arranged by size: 4545 1809 73 AvrII C'CTAG_G Cuts at: 2245 2245 Size: 6427 BamHI G'GATC_C Cuts at: 3757 4124 3757 Size: 367 6060 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3748 5203 618 Size: 325 217 627 1961 1455 1842 Fragments arranged by size: 1961 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3746 1391 Size: 2355 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4618 4652 1903 Size: 34 2681 34 3678 Fragments arranged by size: 3678 2681 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4319 5529 4319 Size: 1210 5217 BclI T'GATC_A Cuts at: 1548 1548 Size: 6427 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2058 5038 136 Size: 107 122 71 631 605 386 2980 1525 Fragments arranged by size: 2980 1525 631 605 386 122 107 71 BglII A'GATC_T Cuts at: 6422 6422 Size: 6427 BlpI GC'TnA_GC Cuts at: 3844 3844 Size: 6427 Bme1580I G_kGCm'C Cuts at: 1790 4488 5644 1790 Size: 2698 1156 2573 Fragments arranged by size: 2698 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6427 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4158 5116 442 Size: 416 53 83 399 24 2741 958 1753 Fragments arranged by size: 2741 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6427 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2752 2752 Size: 6427 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2385 3579 3729 5087 1239 Size: 1146 1194 150 1358 2579 Fragments arranged by size: 2579 1358 1194 1146 150 Bpu10I CC'TnA_GC Cuts at: 2966 2966 Size: 6427 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2442 3405 4552 5417 5715 2442 Size: 963 1147 865 298 3154 Fragments arranged by size: 3154 1147 963 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5090 882 Size: 617 3591 2219 Fragments arranged by size: 3591 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4218 493 Size: 711 3014 2702 Fragments arranged by size: 3014 2702 711 BsaBI GATnn'nnATC Cuts at: 6421 6421 Size: 6427 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4234 4616 5251 275 Size: 53 83 186 936 2701 382 635 1451 Fragments arranged by size: 2701 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4860 5258 5532 5679 6090 4860 Size: 398 274 147 411 5197 Fragments arranged by size: 5197 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2025 2055 2121 2151 3582 Size: 30 165 30 622 30 66 30 1431 Cuts at: 3582 3612 1178 Size: 30 3993 Fragments arranged by size: 3993 1431 622 165 66 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2799 1186 Size: 1613 4814 BseYI C'CCAG_C Cuts at: 5630 6126 5630 Size: 496 5931 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2789 1163 Size: 573 1053 4801 Fragments arranged by size: 4801 1053 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3877 4638 5666 1540 Size: 275 186 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2447 2540 3565 4488 4573 5644 2447 Size: 93 1025 923 85 1071 3230 Fragments arranged by size: 3230 1071 1025 923 93 85 BsmI GAATG_Cn' Cuts at: 2650 2857 3938 4031 2650 Size: 207 1081 93 5046 Fragments arranged by size: 5046 1081 207 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2447 2540 3565 3746 4488 4573 5644 Size: 399 657 93 1025 181 742 85 1071 Cuts at: 5644 1391 Size: 2174 Fragments arranged by size: 2174 1071 1025 742 657 399 181 93 85 BspEI T'CCGG_A Cuts at: 6090 6090 Size: 6427 BspHI T'CATG_A Cuts at: 2173 2521 4316 6046 2173 Size: 348 1795 1730 2554 Fragments arranged by size: 2554 1795 1730 348 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3782 844 Size: 2938 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2036 4314 1394 Size: 429 213 2278 3507 Fragments arranged by size: 3507 2278 429 213 BsrDI GCAATG_nn' Cuts at: 65 1248 3101 4922 5096 65 Size: 1183 1853 1821 174 1396 Fragments arranged by size: 1853 1821 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2052 5071 5258 1700 Size: 110 242 3019 187 2869 Fragments arranged by size: 3019 2869 242 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6427 BssSI C'ACGA_G Cuts at: 4487 5499 4487 Size: 1012 5415 BstAPI GCAn_nnn'nTGC Cuts at: 794 2551 2852 794 Size: 1757 301 4369 Fragments arranged by size: 4369 1757 301 BstBI TT'CG_AA Cuts at: 5231 5231 Size: 6427 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6427 BstYI r'GATC_y Cuts at: 1972 2679 3757 4124 4509 4526 5978 6064 6076 Size: 707 1078 367 385 17 1452 86 12 Cuts at: 6076 6422 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1078 707 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4936 Fragments arranged by size: 4936 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5688 Fragments arranged by size: 5688 683 56 BtsI GCAGTG_nn' Cuts at: 2707 3943 4737 4757 2707 Size: 1236 794 20 4377 Fragments arranged by size: 4377 1236 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6392 DraI TTT'AAA Cuts at: 3731 3731 Size: 6427 DraIII CAC_nnn'GTG Cuts at: 2427 2551 2427 Size: 124 6303 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5434 809 Size: 507 4118 1802 Fragments arranged by size: 4118 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2050 2158 3616 Size: 54 1425 50 275 18 220 108 1458 Cuts at: 3616 3874 4763 6392 8 Size: 258 889 1629 43 Fragments arranged by size: 1629 1458 1425 889 275 258 220 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 1537 Size: 275 2062 4090 Fragments arranged by size: 4090 2062 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2200 2202 4357 1353 Size: 847 2 2155 3423 Fragments arranged by size: 3423 2155 847 2 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5014 5388 5534 387 Size: 1064 138 3425 374 146 1280 Fragments arranged by size: 3425 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2385 3579 3729 4490 5087 5874 6142 Size: 393 753 1194 150 761 597 787 268 Cuts at: 6142 1239 Size: 1524 Fragments arranged by size: 1524 1194 787 761 753 597 393 268 150 EcoRI G'AATT_C Cuts at: 3287 3736 4114 3287 Size: 449 378 5600 Fragments arranged by size: 5600 449 378 EcoRV GAT'ATC Cuts at: 2361 2361 Size: 6427 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2420 2420 Size: 6427 FspI TGC'GCA Cuts at: 4933 4933 Size: 6427 HaeII r_GCGC'y Cuts at: 1678 5574 1678 Size: 3896 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2358 2390 2778 2810 3671 Size: 32 135 32 786 32 388 32 861 Cuts at: 3671 3703 5068 5100 5142 5174 1373 Size: 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1365 861 786 388 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3498 3771 4017 669 Size: 988 281 1560 273 246 3079 Fragments arranged by size: 3079 1560 988 281 273 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6427 HpaI GTT'AAC Cuts at: 3498 4017 3498 Size: 519 5908 KpnI G_GTAC'C Cuts at: 3752 3752 Size: 6427 MfeI C'AATT_G Cuts at: 3309 4026 3309 Size: 717 5710 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2740 3132 3329 3657 5541 5725 1305 Size: 1435 392 197 328 1884 184 2007 Fragments arranged by size: 2007 1884 1435 392 328 197 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2160 10 Size: 54 1425 343 328 4277 Fragments arranged by size: 4277 1425 343 328 54 MslI CAynn'nnrTG Cuts at: 518 1166 2605 2946 4385 4744 4903 518 Size: 648 1439 341 1439 359 159 2042 Fragments arranged by size: 2042 1439 1439 648 359 341 159 MspA1I CmG'CkG Cuts at: 1606 2075 3843 4520 5668 5913 1606 Size: 469 1768 677 1148 245 2120 Fragments arranged by size: 2120 1768 1148 677 469 245 NaeI GCC'GGC Cuts at: 2054 2054 Size: 6427 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4936 Fragments arranged by size: 4936 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6427 NgoMIV G'CCGG_C Cuts at: 2052 2052 Size: 6427 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6427 NotI GC'GGCC_GC Cuts at: 3874 3874 Size: 6427 NspI r_CATG'y Cuts at: 1132 1229 2558 2834 3785 5330 1132 Size: 97 1329 276 951 1545 2229 Fragments arranged by size: 2229 1545 1329 951 276 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6427 PciI A'CATG_T Cuts at: 2830 5326 2830 Size: 2496 3931 PflMI CCAn_nnn'nTGG Cuts at: 1108 3311 1108 Size: 2203 4224 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2121 2153 4496 4528 6009 6041 2121 Size: 32 2343 32 1481 32 2507 Fragments arranged by size: 2507 2343 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6427 PsiI TTA'TAA Cuts at: 3997 3997 Size: 6427 PstI C_TGCA'G Cuts at: 830 2313 2344 2848 3016 3779 830 Size: 1483 31 504 168 763 3478 Fragments arranged by size: 3478 1483 763 504 168 31 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 PvuII CAG'CTG Cuts at: 2075 2075 Size: 6427 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SapI GCTCTTCn'nnn_ Cuts at: 2200 2200 Size: 6427 SbfI CC_TGCA'GG Cuts at: 3779 3779 Size: 6427 ScaI AGT'ACT Cuts at: 4675 6387 4675 Size: 1712 4715 SexAI A'CCwGG_T Cuts at: 2547 2547 Size: 6427 SfcI C'TryA_G Cuts at: 826 1045 2076 2309 2340 2844 3012 3330 3775 Size: 219 1031 233 31 504 168 318 445 Cuts at: 3775 4910 5591 5782 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1031 681 504 445 318 233 219 191 168 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 3754 1872 Size: 1882 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2457 3420 4531 5432 5694 820 Size: 197 926 514 963 1111 901 262 1553 Fragments arranged by size: 1553 1111 963 926 901 514 262 197 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 2558 3785 1132 Size: 1426 1227 3774 Fragments arranged by size: 3774 1426 1227 SspI AAT'ATT Cuts at: 5 52 4351 5 Size: 47 4299 2081 Fragments arranged by size: 4299 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6427 StyI C'CwwG_G Cuts at: 513 1065 2004 2245 3866 513 Size: 552 939 241 1621 3074 Fragments arranged by size: 3074 1621 939 552 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4454 4471 4624 4809 5254 325 Size: 1255 2874 17 153 185 445 1498 Fragments arranged by size: 2874 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4673 6385 96 Size: 274 80 33 51 1443 2696 1712 138 Fragments arranged by size: 2696 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6387 6419 6387 Size: 32 6395 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6427 XbaI T'CTAG_A Cuts at: 3763 3763 Size: 6427 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6427 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6427 XmaI C'CCGG_G Cuts at: 1870 3752 1870 Size: 1882 4545 XmnI GAAnn'nnTTC Cuts at: 4556 4556 Size: 6427 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4235 276 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI