(Circular) (Six-base) MAPSORT of: pFN21AB4699.seq Check: 764 from: 1 to: 5815 pFN21AB4699 5815 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3625 278 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2607 3158 1937 Size: 670 551 4594 Fragments arranged by size: 4594 670 551 Acc65I G'GTAC_C Cuts at: 3136 3136 Size: 5815 AclI AA'CG_TT Cuts at: 1563 3942 4315 1563 Size: 2379 373 3063 Fragments arranged by size: 3063 2379 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2524 3878 5262 5530 1632 Size: 892 1354 1384 268 1917 Fragments arranged by size: 1917 1384 1354 892 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5618 AflIII A'CryG_T Cuts at: 1203 2414 4714 5691 1203 Size: 1211 2300 977 1327 Fragments arranged by size: 2300 1327 1211 977 AgeI A'CCGG_T Cuts at: 4646 4646 Size: 5815 AhdI GACnn_n'nnGTC Cuts at: 4544 4544 Size: 5815 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5815 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5815 AlwNI CAG_nnn'CTG Cuts at: 2975 3558 5130 2975 Size: 583 1572 3660 Fragments arranged by size: 3660 1572 583 ApaI G_GGCC'C Cuts at: 2903 2903 Size: 5815 ApaLI G'TGCA_C Cuts at: 3872 5028 3872 Size: 1156 4659 ApoI r'AATT_y Cuts at: 1493 3124 3350 3502 5611 1493 Size: 1631 226 152 2109 1697 Fragments arranged by size: 2109 1697 1631 226 152 AseI AT'TA_AT Cuts at: 160 1032 4369 160 Size: 872 3337 1606 Fragments arranged by size: 3337 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 AvaI C'yCGr_G Cuts at: 1870 1943 3140 1870 Size: 73 1197 4545 Fragments arranged by size: 4545 1197 73 BamHI G'GATC_C Cuts at: 3145 3512 3145 Size: 367 5448 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3136 4591 618 Size: 325 217 627 1349 1455 1842 Fragments arranged by size: 1842 1455 1349 627 325 217 BanII G_rGCy'C Cuts at: 1391 2903 3134 1391 Size: 1512 231 4072 Fragments arranged by size: 4072 1512 231 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4844 BbvCI CC'TCA_GC Cuts at: 3041 3041 Size: 5815 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4006 4040 1903 Size: 34 2069 34 3678 Fragments arranged by size: 3678 2069 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3707 4917 3707 Size: 1210 4605 BclI T'GATC_A Cuts at: 1548 1548 Size: 5815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4426 136 Size: 107 122 71 631 605 2754 1525 Fragments arranged by size: 2754 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2210 5810 2210 Size: 3600 2215 BlpI GC'TnA_GC Cuts at: 3232 3232 Size: 5815 Bme1580I G_kGCm'C Cuts at: 1790 2489 2903 3876 5032 1790 Size: 699 414 973 1156 2573 Fragments arranged by size: 2573 1156 973 699 414 BmgBI CAC'GTC Cuts at: 1318 2415 2482 1318 Size: 1097 67 4651 Fragments arranged by size: 4651 1097 67 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3546 4504 442 Size: 416 53 83 399 24 2129 958 1753 Fragments arranged by size: 2129 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2738 1056 Size: 1682 4133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2724 4475 1239 Size: 1485 1751 2579 Fragments arranged by size: 2579 1751 1485 Bpu10I CC'TnA_GC Cuts at: 3041 3041 Size: 5815 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2597 3940 4805 5103 2597 Size: 1343 865 298 3309 Fragments arranged by size: 3309 1343 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4478 882 Size: 617 2979 2219 Fragments arranged by size: 2979 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3606 493 Size: 711 2402 2702 Fragments arranged by size: 2702 2402 711 BsaBI GATnn'nnATC Cuts at: 5809 5809 Size: 5815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3622 4004 4639 275 Size: 53 83 186 936 2089 382 635 1451 Fragments arranged by size: 2089 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4248 4646 4920 5067 5478 4248 Size: 398 274 147 411 4585 Fragments arranged by size: 4585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2091 2121 1178 Size: 30 165 30 688 30 4872 Fragments arranged by size: 4872 688 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5815 BseYI C'CCAG_C Cuts at: 2773 5018 5514 2773 Size: 2245 496 3074 Fragments arranged by size: 3074 2245 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5242 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2638 3265 4026 5054 1540 Size: 275 186 637 627 761 1028 2301 Fragments arranged by size: 2301 1028 761 637 627 275 186 BsiHKAI G_wGCw'C Cuts at: 2167 3876 3961 5032 2167 Size: 1709 85 1071 2950 Fragments arranged by size: 2950 1709 1071 85 BsmI GAATG_Cn' Cuts at: 3326 3419 3326 Size: 93 5722 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2167 2489 2903 3134 3876 3961 5032 Size: 399 377 322 414 231 742 85 1071 Cuts at: 5032 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 414 399 377 322 231 85 BspEI T'CCGG_A Cuts at: 5478 5478 Size: 5815 BspHI T'CATG_A Cuts at: 3704 5434 3704 Size: 1730 4085 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3170 844 Size: 2326 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2535 3702 1394 Size: 429 712 1167 3507 Fragments arranged by size: 3507 1167 712 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4310 4484 65 Size: 1183 3062 174 1396 Fragments arranged by size: 3062 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4459 4646 1700 Size: 110 2649 187 2869 Fragments arranged by size: 2869 2649 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5815 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5815 BssSI C'ACGA_G Cuts at: 3875 4887 3875 Size: 1012 4803 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5815 BstBI TT'CG_AA Cuts at: 4619 4619 Size: 5815 BstEII G'GTnAC_C Cuts at: 1184 3087 1184 Size: 1903 3912 BstXI CCAn_nnnn'nTGG Cuts at: 2773 2773 Size: 5815 BstYI r'GATC_y Cuts at: 1972 2185 2210 3145 3512 3897 3914 5366 5452 Size: 213 25 935 367 385 17 1452 86 Cuts at: 5452 5464 5810 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 935 385 367 346 213 86 25 17 12 Bsu36I CC'TnA_GG Cuts at: 2557 2557 Size: 5815 BtgI C'CryG_G Cuts at: 513 1065 2004 2457 513 Size: 552 939 453 3871 Fragments arranged by size: 3871 939 552 453 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5076 Fragments arranged by size: 5076 683 56 BtsI GCAGTG_nn' Cuts at: 2325 3331 4125 4145 2325 Size: 1006 794 20 3995 Fragments arranged by size: 3995 1006 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2584 2619 2751 2786 529 Size: 35 2020 35 132 35 3558 Fragments arranged by size: 3558 2020 132 35 35 35 DraI TTT'AAA Cuts at: 3119 3119 Size: 5815 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4822 809 Size: 507 3506 1802 Fragments arranged by size: 3506 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2454 2713 3262 Size: 54 1425 50 275 18 624 259 549 Cuts at: 3262 4151 5780 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 624 549 275 259 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3262 1537 Size: 275 1450 4090 Fragments arranged by size: 4090 1450 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2345 3745 1353 Size: 992 1400 3423 Fragments arranged by size: 3423 1400 992 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4402 4776 4922 387 Size: 1064 138 2813 374 146 1280 Fragments arranged by size: 2813 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2524 2724 3878 4475 5262 5530 1239 Size: 393 892 200 1154 597 787 268 1524 Fragments arranged by size: 1524 1154 892 787 597 393 268 200 EcoO109I rG'GnC_Cy Cuts at: 2753 2899 2900 2942 2753 Size: 146 1 42 5626 Fragments arranged by size: 5626 146 42 1 EcoRI G'AATT_C Cuts at: 3124 3502 3124 Size: 378 5437 EcoRV GAT'ATC Cuts at: 2203 2203 Size: 5815 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3117 3117 Size: 5815 FspI TGC'GCA Cuts at: 4321 4321 Size: 5815 HaeII r_GCGC'y Cuts at: 1678 3049 4962 1678 Size: 1371 1913 2531 Fragments arranged by size: 2531 1913 1371 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4456 4488 4530 4562 1373 Size: 32 135 32 2884 32 42 32 2626 Fragments arranged by size: 2884 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3159 3405 669 Size: 988 281 1221 246 3079 Fragments arranged by size: 3079 1221 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5815 HpaI GTT'AAC Cuts at: 3405 3405 Size: 5815 KpnI G_GTAC'C Cuts at: 3140 3140 Size: 5815 MfeI C'AATT_G Cuts at: 2728 3414 2728 Size: 686 5129 MluI A'CGCG_T Cuts at: 5691 5691 Size: 5815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2163 2551 4929 5113 1305 Size: 858 388 2378 184 2007 Fragments arranged by size: 2378 2007 858 388 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2456 2715 10 Size: 54 1425 343 624 259 3110 Fragments arranged by size: 3110 1425 624 343 259 54 MslI CAynn'nnrTG Cuts at: 518 1166 3773 4132 4291 518 Size: 648 2607 359 159 2042 Fragments arranged by size: 2607 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 2723 2972 3231 3908 5056 5301 1606 Size: 421 696 249 259 677 1148 245 2120 Fragments arranged by size: 2120 1148 696 677 421 259 249 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2457 513 Size: 552 939 453 3871 Fragments arranged by size: 3871 939 552 453 NdeI CA'TA_TG Cuts at: 387 387 Size: 5815 NheI G'CTAG_C Cuts at: 1052 2734 1052 Size: 1682 4133 NotI GC'GGCC_GC Cuts at: 3262 3262 Size: 5815 NspI r_CATG'y Cuts at: 1132 1229 3173 4718 1132 Size: 97 1944 1545 2229 Fragments arranged by size: 2229 1944 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5815 PciI A'CATG_T Cuts at: 4714 4714 Size: 5815 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5815 PfoI T'CCnGG_A Cuts at: 2701 2701 Size: 5815 PmeI GTTT'AAAC Cuts at: 3119 3119 Size: 5815 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3884 3916 5397 5429 3884 Size: 32 1481 32 4270 Fragments arranged by size: 4270 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2753 2753 Size: 5815 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5815 PsiI TTA'TAA Cuts at: 3385 3385 Size: 5815 PspOMI G'GGCC_C Cuts at: 2899 2899 Size: 5815 PstI C_TGCA'G Cuts at: 830 3077 3167 830 Size: 2247 90 3478 Fragments arranged by size: 3478 2247 90 PvuI CG_AT'CG Cuts at: 2001 2638 2001 Size: 637 5178 PvuII CAG'CTG Cuts at: 2027 2723 2972 2027 Size: 696 249 4870 Fragments arranged by size: 4870 696 249 SalI G'TCGA_C Cuts at: 1936 3157 1936 Size: 1221 4594 SanDI GG'GwC_CC Cuts at: 2753 2753 Size: 5815 SapI GCTCTTCn'nnn_ Cuts at: 2345 2345 Size: 5815 SbfI CC_TGCA'GG Cuts at: 3167 3167 Size: 5815 ScaI AGT'ACT Cuts at: 4063 5775 4063 Size: 1712 4103 SexAI A'CCwGG_T Cuts at: 2907 2907 Size: 5815 SfcI C'TryA_G Cuts at: 826 1045 2014 2946 3073 3163 4298 4979 5170 Size: 219 969 932 127 90 1135 681 191 Cuts at: 5170 826 Size: 1471 Fragments arranged by size: 1471 1135 969 932 681 219 191 127 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5815 SmaI CCC'GGG Cuts at: 1872 3142 1872 Size: 1270 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2612 3919 4820 5082 820 Size: 197 926 669 1307 901 262 1553 Fragments arranged by size: 1553 1307 926 901 669 262 197 SnaBI TAC'GTA Cuts at: 493 3606 493 Size: 3113 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5815 SphI G_CATG'C Cuts at: 1132 3173 1132 Size: 2041 3774 SspI AAT'ATT Cuts at: 5 52 3739 5 Size: 47 3687 2081 Fragments arranged by size: 3687 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5815 StyI C'CwwG_G Cuts at: 513 1065 2004 2082 2457 3092 3254 513 Size: 552 939 78 375 635 162 3074 Fragments arranged by size: 3074 939 635 552 375 162 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2779 3842 3859 4012 4197 4642 325 Size: 1255 1199 1063 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1199 1063 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4061 5773 96 Size: 274 80 33 51 1443 2084 1712 138 Fragments arranged by size: 2084 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2091 2123 5775 5807 2091 Size: 32 3652 32 2099 Fragments arranged by size: 3652 2099 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2446 1316 Size: 1130 4685 XbaI T'CTAG_A Cuts at: 3151 3151 Size: 5815 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5815 XmaI C'CCGG_G Cuts at: 1870 3140 1870 Size: 1270 4545 XmnI GAAnn'nnTTC Cuts at: 3944 3944 Size: 5815 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3623 276 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI XcmI