Restriction map for
pFN21AB4688
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BbeI
BbrPI BbvCI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MluI MroI
MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 143 GTAGAC GT^[AC][GT]AC
AccII 941 CGCG CG^CG
AlwNI 1231 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 473 GGATCC G^GATCC
BanII 487 GAGCCC G[GA]GC[CT]^C
BbsI 1110 GAAGAC GAAGAC(2/6)
BcgI 450 CGATGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1586 TGATCA T^GATCA
BglI 495 GCCAGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1110 GAAGAC GAAGAC(2/6)
BsaAI 1438 CACGTA [CT]AC^GT[GA]
BsgI 194 GTGCAG GTGCAG(16/14)
BsrDI 1025 GCAATG GCAATG(2/0)
BssSI 1458 CACGAG CACGAG(-5/-1)
BstUI 941 CGCG CG^CG
DraI 1413 TTTAAA TTT^AAA
EcoRI 1247 GAATTC G^AATTC
FbaI 1586 TGATCA T^GATCA
HapII 615 CCGG C^CGG
HincII 277 GTTGAC GT[CT]^[GA]AC
HindII 277 GTTGAC GT[CT]^[GA]AC
HpaII 615 CCGG C^CGG
MaeII 1439 ACGT A^CGT
MfeI 1563 CAATTG C^AATTG
MspI 615 CCGG C^CGG
MunI 1563 CAATTG C^AATTG
MvnI 941 CGCG CG^CG
NspI 1040 ACATGC [GA]CATG^[CT]
PshAI 1706 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 614 CCCGGG CCC^GGG
SpeI 1186 ACTAGT A^CTAGT
StuI 933 AGGCCT AGG^CCT
TaqI 605 TCGA T^CGA
Tsp45I 649 GTGAC ^GT[GC]AC
TthHB8I 605 TCGA T^CGA
XmaCI 614 CCCGGG C^CCGGG
XmaI 614 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BalI 843 TGGCCA TGG^CCA
1016 TGGCCA
BcnI 614 CCCGG CC^[GC]GG
615 CCGGG
BpmI 505 CTGGAG CTGGAG(16/14)
1045 CTGGAG
BsaI 1405 GAGACC GGTCTC(1/5)
1461 GAGACC
BsmFI 1183 GGGAC GGGAC(10/14)
1066 GTCCC
BspMI 139 GCAGGT ACCTGC(4/8)
963 GCAGGT
EaeI 843 TGGCCA [CT]^GGCC[GA]
1016 TGGCCA
MluNI 843 TGGCCA TGG^CCA
1016 TGGCCA
MscI 843 TGGCCA TGG^CCA
1016 TGGCCA
MspA1I 197 CAGCTG C[AC]G^C[GT]G
1234 CAGCTG
NciI 614 CCCGG CC^[GC]GG
615 CCGGG
PleI 1493 GAGTC GAGTC(4/5)
1615 GAGTC
PvuII 197 CAGCTG CAG^CTG
1234 CAGCTG
XcmI 1004 CCAACAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1437 CCACGTACCCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BanI BbvI
BfaI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PflMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp509I
TspRI Van91I XhoII