(Circular) (Six-base) MAPSORT of: pFN21AB4688.seq Check: 8620 from: 1 to: 6436 pFN21AB4688 6436 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2959 2959 Size: 6436 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4246 278 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2149 3779 1937 Size: 212 1630 4594 Fragments arranged by size: 4594 1630 212 Acc65I G'GTAC_C Cuts at: 3757 3757 Size: 6436 AclI AA'CG_TT Cuts at: 1563 4563 4936 1563 Size: 3000 373 3063 Fragments arranged by size: 3063 3000 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2771 3420 4499 5883 6151 1632 Size: 1139 649 1079 1384 268 1917 Fragments arranged by size: 1917 1384 1139 1079 649 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6239 AflIII A'CryG_T Cuts at: 1203 5335 6312 1203 Size: 4132 977 1327 Fragments arranged by size: 4132 1327 977 AgeI A'CCGG_T Cuts at: 5267 5267 Size: 6436 AhdI GACnn_n'nnGTC Cuts at: 5165 5165 Size: 6436 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6436 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3261 1184 Size: 2077 4359 AlwNI CAG_nnn'CTG Cuts at: 3241 4179 5751 3241 Size: 938 1572 3926 Fragments arranged by size: 3926 1572 938 ApaLI G'TGCA_C Cuts at: 4493 5649 4493 Size: 1156 5280 ApoI r'AATT_y Cuts at: 1493 2605 3252 3551 3745 3971 4123 6232 1493 Size: 1112 647 299 194 226 152 2109 1697 Fragments arranged by size: 2109 1697 1112 647 299 226 194 152 AseI AT'TA_AT Cuts at: 160 1032 4990 160 Size: 872 3958 1606 Fragments arranged by size: 3958 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 AvaI C'yCGr_G Cuts at: 1870 1943 2344 2619 3269 3372 3761 1870 Size: 73 401 275 650 103 389 4545 Fragments arranged by size: 4545 650 401 389 275 103 73 BamHI G'GATC_C Cuts at: 2478 3766 4133 2478 Size: 1288 367 4781 Fragments arranged by size: 4781 1288 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3005 3265 3392 3757 5212 Size: 325 217 627 1218 260 127 365 1455 Cuts at: 5212 618 Size: 1842 Fragments arranged by size: 1842 1455 1218 627 365 325 260 217 127 BanII G_rGCy'C Cuts at: 1391 2496 3755 1391 Size: 1105 1259 4072 Fragments arranged by size: 4072 1259 1105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3122 928 Size: 971 1223 4242 Fragments arranged by size: 4242 1223 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2444 2478 4627 4661 1903 Size: 34 507 34 2149 34 3678 Fragments arranged by size: 3678 2149 507 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4328 5538 4328 Size: 1210 5226 BclI T'GATC_A Cuts at: 1548 3591 1548 Size: 2043 4393 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2506 5047 136 Size: 107 122 71 631 605 834 2541 1525 Fragments arranged by size: 2541 1525 834 631 605 122 107 71 BglII A'GATC_T Cuts at: 6431 6431 Size: 6436 BlpI GC'TnA_GC Cuts at: 3853 3853 Size: 6436 Bme1580I G_kGCm'C Cuts at: 1790 3008 3270 3395 4497 5653 1790 Size: 1218 262 125 1102 1156 2573 Fragments arranged by size: 2573 1218 1156 1102 262 125 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6436 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4167 5125 442 Size: 416 53 83 399 24 2750 958 1753 Fragments arranged by size: 2750 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6436 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 3071 5096 1239 Size: 1292 540 2025 2579 Fragments arranged by size: 2579 2025 1292 540 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1995 2762 3086 4561 5426 5724 1995 Size: 767 324 1475 865 298 2707 Fragments arranged by size: 2707 1475 865 767 324 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3404 3460 5099 882 Size: 617 1905 56 1639 2219 Fragments arranged by size: 2219 1905 1639 617 56 BsaAI yAC'GTr Cuts at: 493 1204 3445 4227 493 Size: 711 2241 782 2702 Fragments arranged by size: 2702 2241 782 711 BsaBI GATnn'nnATC Cuts at: 6430 6430 Size: 6436 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4243 4625 5260 275 Size: 53 83 186 936 2710 382 635 1451 Fragments arranged by size: 2710 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4869 5267 5541 5688 6099 4869 Size: 398 274 147 411 5206 Fragments arranged by size: 5206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2900 2930 1178 Size: 30 165 30 1497 30 4684 Fragments arranged by size: 4684 1497 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2343 2759 2915 3125 3565 1186 Size: 1157 416 156 210 440 4057 Fragments arranged by size: 4057 1157 440 416 210 156 BseYI C'CCAG_C Cuts at: 5639 6135 5639 Size: 496 5940 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2220 1163 Size: 573 484 5379 Fragments arranged by size: 5379 573 484 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3886 4647 5675 1540 Size: 275 186 1885 761 1028 2301 Fragments arranged by size: 2301 1885 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4497 4582 5653 4497 Size: 85 1071 5280 Fragments arranged by size: 5280 1071 85 BsmI GAATG_Cn' Cuts at: 3947 4040 3947 Size: 93 6343 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2496 3008 3270 3395 3755 4497 4582 Size: 399 706 512 262 125 360 742 85 Cuts at: 4582 5653 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 706 512 399 360 262 125 85 BspEI T'CCGG_A Cuts at: 6099 6099 Size: 6436 BspHI T'CATG_A Cuts at: 4325 6055 4325 Size: 1730 4706 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2135 2959 3791 844 Size: 1291 824 832 3489 Fragments arranged by size: 3489 1291 832 824 BsrBI CCG'CTC Cuts at: 1394 1823 4323 1394 Size: 429 2500 3507 Fragments arranged by size: 3507 2500 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3037 4931 5105 65 Size: 1183 1789 1894 174 1396 Fragments arranged by size: 1894 1789 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5080 5267 1700 Size: 110 3270 187 2869 Fragments arranged by size: 3270 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6436 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6436 BssSI C'ACGA_G Cuts at: 3463 4496 5508 3463 Size: 1033 1012 4391 Fragments arranged by size: 4391 1033 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6436 BstBI TT'CG_AA Cuts at: 5240 5240 Size: 6436 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6436 BstYI r'GATC_y Cuts at: 1972 2478 2883 3054 3766 4133 4518 4535 5987 Size: 506 405 171 712 367 385 17 1452 Cuts at: 5987 6073 6085 6431 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 712 506 405 385 367 346 171 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3261 3351 3451 3598 513 Size: 552 2196 90 100 147 3351 Fragments arranged by size: 3351 2196 552 147 100 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5697 Fragments arranged by size: 5697 683 56 BtsI GCAGTG_nn' Cuts at: 2988 3952 4746 4766 2988 Size: 964 794 20 4658 Fragments arranged by size: 4658 964 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6436 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6401 DraI TTT'AAA Cuts at: 3420 3740 3420 Size: 320 6116 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5443 809 Size: 507 4127 1802 Fragments arranged by size: 4127 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2848 3021 3883 Size: 54 1425 50 275 18 1018 173 862 Cuts at: 3883 4772 6401 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1018 889 862 275 173 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3883 1537 Size: 275 2071 4090 Fragments arranged by size: 4090 2071 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2088 2518 3439 4366 1353 Size: 735 430 921 927 3423 Fragments arranged by size: 3423 927 921 735 430 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5023 5397 5543 387 Size: 1064 138 3434 374 146 1280 Fragments arranged by size: 3434 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2531 2771 3071 3420 4499 5096 5883 Size: 393 899 240 300 349 1079 597 787 Cuts at: 5883 6151 1239 Size: 268 1524 Fragments arranged by size: 1524 1079 899 787 597 393 349 300 268 240 EcoRI G'AATT_C Cuts at: 3252 3745 4123 3252 Size: 493 378 5565 Fragments arranged by size: 5565 493 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2411 2411 Size: 6436 FspI TGC'GCA Cuts at: 4942 4942 Size: 6436 HaeII r_GCGC'y Cuts at: 1678 5583 1678 Size: 3905 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 3208 3240 3605 Size: 32 135 32 704 32 900 32 365 Cuts at: 3605 3637 5077 5109 5151 5183 1373 Size: 32 1440 32 42 32 2626 Fragments arranged by size: 2626 1440 900 704 365 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2284 3780 4026 669 Size: 988 281 346 1496 246 3079 Fragments arranged by size: 3079 1496 988 346 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6436 HpaI GTT'AAC Cuts at: 4026 4026 Size: 6436 KpnI G_GTAC'C Cuts at: 3761 3761 Size: 6436 MfeI C'AATT_G Cuts at: 3568 4035 3568 Size: 467 5969 MluI A'CGCG_T Cuts at: 6312 6312 Size: 6436 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2959 3651 5550 5734 1305 Size: 1654 692 1899 184 2007 Fragments arranged by size: 2007 1899 1654 692 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2850 3023 10 Size: 54 1425 343 1018 173 3423 Fragments arranged by size: 3423 1425 1018 343 173 54 MslI CAynn'nnrTG Cuts at: 518 1166 4394 4753 4912 518 Size: 648 3228 359 159 2042 Fragments arranged by size: 3228 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2204 3241 3852 4529 5677 5922 1606 Size: 598 1037 611 677 1148 245 2120 Fragments arranged by size: 2120 1148 1037 677 611 598 245 NcoI C'CATG_G Cuts at: 513 1065 3261 3451 3598 513 Size: 552 2196 190 147 3351 Fragments arranged by size: 3351 2196 552 190 147 NdeI CA'TA_TG Cuts at: 387 387 Size: 6436 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6436 NotI GC'GGCC_GC Cuts at: 3883 3883 Size: 6436 NspI r_CATG'y Cuts at: 1132 1229 3049 3794 5339 1132 Size: 97 1820 745 1545 2229 Fragments arranged by size: 2229 1820 1545 745 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6436 PciI A'CATG_T Cuts at: 5335 5335 Size: 6436 PflMI CCAn_nnn'nTGG Cuts at: 1108 2846 3140 3634 1108 Size: 1738 294 494 3910 Fragments arranged by size: 3910 1738 494 294 PmeI GTTT'AAAC Cuts at: 3740 3740 Size: 6436 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3194 3226 4505 4537 6018 6050 3194 Size: 32 1279 32 1481 32 3580 Fragments arranged by size: 3580 1481 1279 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3715 1613 Size: 2102 4334 PsiI TTA'TAA Cuts at: 4006 4006 Size: 6436 PstI C_TGCA'G Cuts at: 830 2209 2487 2776 2995 3788 830 Size: 1379 278 289 219 793 3478 Fragments arranged by size: 3478 1379 793 289 278 219 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6436 PvuII CAG'CTG Cuts at: 2204 3241 2204 Size: 1037 5399 SalI G'TCGA_C Cuts at: 1936 3778 1936 Size: 1842 4594 SbfI CC_TGCA'GG Cuts at: 3788 3788 Size: 6436 ScaI AGT'ACT Cuts at: 4684 6396 4684 Size: 1712 4724 SfcI C'TryA_G Cuts at: 826 1045 2205 2226 2483 2772 2991 3285 3784 Size: 219 1160 21 257 289 219 294 499 Cuts at: 3784 4919 5600 5791 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1160 1135 681 499 294 289 257 219 219 191 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6436 SmaI CCC'GGG Cuts at: 1872 2621 3763 1872 Size: 749 1142 4545 Fragments arranged by size: 4545 1142 749 SmlI C'TyrA_G Cuts at: 820 1017 1943 2010 2741 3101 4540 5441 5703 Size: 197 926 67 731 360 1439 901 262 Cuts at: 5703 820 Size: 1553 Fragments arranged by size: 1553 1439 926 901 731 360 262 197 67 SnaBI TAC'GTA Cuts at: 493 4227 493 Size: 3734 2702 SpeI A'CTAG_T Cuts at: 152 3191 152 Size: 3039 3397 SphI G_CATG'C Cuts at: 1132 3794 1132 Size: 2662 3774 SspI AAT'ATT Cuts at: 5 52 4360 5 Size: 47 4308 2081 Fragments arranged by size: 4308 2081 47 StuI AGG'CCT Cuts at: 1517 2940 1517 Size: 1423 5013 StyI C'CwwG_G Cuts at: 513 1065 2658 3058 3261 3345 3451 3598 3875 Size: 552 1593 400 203 84 106 147 277 Cuts at: 3875 513 Size: 3074 Fragments arranged by size: 3074 1593 552 400 277 203 147 106 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3136 4463 4480 4633 4818 5263 325 Size: 1255 1556 1327 17 153 185 445 1498 Fragments arranged by size: 1556 1498 1327 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2916 3683 4682 Size: 274 80 33 51 1443 939 767 999 Cuts at: 4682 6394 96 Size: 1712 138 Fragments arranged by size: 1712 1443 999 939 767 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6396 6428 6396 Size: 32 6404 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6436 XbaI T'CTAG_A Cuts at: 3772 3772 Size: 6436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3016 3449 3016 Size: 433 6003 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6436 XmaI C'CCGG_G Cuts at: 1870 2619 3761 1870 Size: 749 1142 4545 Fragments arranged by size: 4545 1142 749 XmnI GAAnn'nnTTC Cuts at: 4565 4565 Size: 6436 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4244 276 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI