(Circular) (Six-base) MAPSORT of: pFN21AB4680.seq Check: 4512 from: 1 to: 6271 pFN21AB4680 6271 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2806 2806 Size: 6271 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4081 278 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2026 3614 1937 Size: 89 1588 4594 Fragments arranged by size: 4594 1588 89 Acc65I G'GTAC_C Cuts at: 3592 3592 Size: 6271 AclI AA'CG_TT Cuts at: 1563 4398 4771 1563 Size: 2835 373 3063 Fragments arranged by size: 3063 2835 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4334 5718 5986 1632 Size: 2702 1384 268 1917 Fragments arranged by size: 2702 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6074 AflIII A'CryG_T Cuts at: 1203 2319 2550 5170 6147 1203 Size: 1116 231 2620 977 1327 Fragments arranged by size: 2620 1327 1116 977 231 AgeI A'CCGG_T Cuts at: 5102 5102 Size: 6271 AhdI GACnn_n'nnGTC Cuts at: 5000 5000 Size: 6271 AleI CACnn'nnGTG Cuts at: 1166 2690 1166 Size: 1524 4747 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2828 1184 Size: 1644 4627 AlwNI CAG_nnn'CTG Cuts at: 2044 2627 2649 3534 4014 5586 2044 Size: 583 22 885 480 1572 2729 Fragments arranged by size: 2729 1572 885 583 480 22 ApaLI G'TGCA_C Cuts at: 3380 4328 5484 3380 Size: 948 1156 4167 Fragments arranged by size: 4167 1156 948 ApoI r'AATT_y Cuts at: 1493 2243 3580 3806 3958 6067 1493 Size: 750 1337 226 152 2109 1697 Fragments arranged by size: 2109 1697 1337 750 226 152 AseI AT'TA_AT Cuts at: 160 1032 4825 160 Size: 872 3793 1606 Fragments arranged by size: 3793 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 AvaI C'yCGr_G Cuts at: 1870 1943 3301 3596 1870 Size: 73 1358 295 4545 Fragments arranged by size: 4545 1358 295 73 BamHI G'GATC_C Cuts at: 3601 3968 3601 Size: 367 5904 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3592 5047 618 Size: 325 217 627 1805 1455 1842 Fragments arranged by size: 1842 1805 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3098 3590 1391 Size: 1707 492 4072 Fragments arranged by size: 4072 1707 492 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2295 3130 3260 928 Size: 971 396 835 130 3939 Fragments arranged by size: 3939 971 835 396 130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4462 4496 1903 Size: 34 2525 34 3678 Fragments arranged by size: 3678 2525 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4163 5373 4163 Size: 1210 5061 BclI T'GATC_A Cuts at: 1548 1548 Size: 6271 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2945 3102 4882 Size: 107 122 71 631 605 1273 157 1780 Cuts at: 4882 136 Size: 1525 Fragments arranged by size: 1780 1525 1273 631 605 157 122 107 71 BglII A'GATC_T Cuts at: 3108 6266 3108 Size: 3158 3113 BlpI GC'TnA_GC Cuts at: 3688 3688 Size: 6271 Bme1580I G_kGCm'C Cuts at: 1790 3122 3234 3384 4332 5488 1790 Size: 1332 112 150 948 1156 2573 Fragments arranged by size: 2573 1332 1156 948 150 112 BmgBI CAC'GTC Cuts at: 1318 2293 2794 1318 Size: 975 501 4795 Fragments arranged by size: 4795 975 501 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2098 2466 3322 Size: 416 53 83 399 24 681 368 856 Cuts at: 3322 4002 4960 442 Size: 680 958 1753 Fragments arranged by size: 1753 958 856 681 680 416 399 368 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2300 1056 Size: 1244 5027 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2452 2452 Size: 6271 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 4931 1239 Size: 1010 2682 2579 Fragments arranged by size: 2682 2579 1010 Bpu10I CC'TnA_GC Cuts at: 3092 3170 3362 3092 Size: 78 192 6001 Fragments arranged by size: 6001 192 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2992 3263 3540 4396 5261 5559 2992 Size: 271 277 856 865 298 3704 Fragments arranged by size: 3704 865 856 298 277 271 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4934 882 Size: 617 3435 2219 Fragments arranged by size: 3435 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3237 4062 493 Size: 711 2033 825 2702 Fragments arranged by size: 2702 2033 825 711 BsaBI GATnn'nnATC Cuts at: 6265 6265 Size: 6271 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4078 4460 5095 275 Size: 53 83 186 936 2545 382 635 1451 Fragments arranged by size: 2545 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4704 5102 5376 5523 5934 4704 Size: 398 274 147 411 5041 Fragments arranged by size: 5041 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6046 Fragments arranged by size: 6046 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6271 BseYI C'CCAG_C Cuts at: 2516 3143 5474 5970 2516 Size: 627 2331 496 2817 Fragments arranged by size: 2817 2331 627 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2065 2834 1163 Size: 573 329 769 4600 Fragments arranged by size: 4600 769 573 329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2538 3721 4482 5510 1540 Size: 275 186 537 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 537 275 186 BsiHKAI G_wGCw'C Cuts at: 2912 3176 3242 3384 4332 4417 5488 2912 Size: 264 66 142 948 85 1071 3695 Fragments arranged by size: 3695 1071 948 264 142 85 66 BsmI GAATG_Cn' Cuts at: 3782 3875 3782 Size: 93 6178 BsmBI CGTCTCn'nnnn_ Cuts at: 3085 3085 Size: 6271 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2912 3098 3122 3176 3234 3242 3384 Size: 399 1122 186 24 54 58 8 142 Cuts at: 3384 3590 4332 4417 5488 1391 Size: 206 742 85 1071 2174 Fragments arranged by size: 2174 1122 1071 742 399 206 186 142 85 58 54 24 8 BspEI T'CCGG_A Cuts at: 5934 5934 Size: 6271 BspHI T'CATG_A Cuts at: 4160 5890 4160 Size: 1730 4541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2806 3414 3626 844 Size: 1962 608 212 3489 Fragments arranged by size: 3489 1962 608 212 BsrBI CCG'CTC Cuts at: 1394 1823 4158 1394 Size: 429 2335 3507 Fragments arranged by size: 3507 2335 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4766 4940 65 Size: 1183 3518 174 1396 Fragments arranged by size: 3518 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2935 4915 5102 1700 Size: 110 1125 1980 187 2869 Fragments arranged by size: 2869 1980 1125 187 110 BsrGI T'GTAC_A Cuts at: 96 3270 3447 96 Size: 3174 177 2920 Fragments arranged by size: 3174 2920 177 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6271 BssSI C'ACGA_G Cuts at: 3287 4331 5343 3287 Size: 1044 1012 4215 Fragments arranged by size: 4215 1044 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3330 3414 794 Size: 2536 84 3651 Fragments arranged by size: 3651 2536 84 BstBI TT'CG_AA Cuts at: 5075 5075 Size: 6271 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6271 BstYI r'GATC_y Cuts at: 1972 3108 3162 3601 3968 4353 4370 5822 5908 Size: 1136 54 439 367 385 17 1452 86 Cuts at: 5908 5920 6266 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1136 439 385 367 346 86 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2954 2954 Size: 6271 BtgI C'CryG_G Cuts at: 513 1065 2004 2370 3099 3375 513 Size: 552 939 366 729 276 3409 Fragments arranged by size: 3409 939 729 552 366 276 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5532 Fragments arranged by size: 5532 683 56 BtsI GCAGTG_nn' Cuts at: 3787 4581 4601 3787 Size: 794 20 5457 Fragments arranged by size: 5457 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6236 DraI TTT'AAA Cuts at: 3575 3575 Size: 6271 DraIII CAC_nnn'GTG Cuts at: 3330 3330 Size: 6271 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2732 5278 809 Size: 507 1416 2546 1802 Fragments arranged by size: 2546 1802 1416 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2535 3718 4607 Size: 54 1425 50 275 18 705 1183 889 Cuts at: 4607 6236 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1183 889 705 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2535 3718 1537 Size: 275 723 1183 4090 Fragments arranged by size: 4090 1183 723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2788 4201 1353 Size: 1435 1413 3423 Fragments arranged by size: 3423 1435 1413 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2613 4858 5232 5378 387 Size: 1064 138 1024 2245 374 146 1280 Fragments arranged by size: 2245 1280 1064 1024 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 4334 4931 5718 5986 1239 Size: 393 617 2085 597 787 268 1524 Fragments arranged by size: 2085 1524 787 617 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2692 2813 2957 2692 Size: 121 144 6006 Fragments arranged by size: 6006 144 121 EcoO109I rG'GnC_Cy Cuts at: 3442 3442 Size: 6271 EcoRI G'AATT_C Cuts at: 2243 3580 3958 2243 Size: 1337 378 4556 Fragments arranged by size: 4556 1337 378 FspI TGC'GCA Cuts at: 4777 4777 Size: 6271 HaeII r_GCGC'y Cuts at: 1678 3286 5418 1678 Size: 1608 2132 2531 Fragments arranged by size: 2531 2132 1608 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2654 2686 4912 4944 4986 Size: 32 135 32 1082 32 2226 32 42 Cuts at: 4986 5018 1373 Size: 32 2626 Fragments arranged by size: 2626 2226 1082 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2713 3226 3615 3861 669 Size: 988 281 775 513 389 246 3079 Fragments arranged by size: 3079 988 775 513 389 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6271 HpaI GTT'AAC Cuts at: 3861 3861 Size: 6271 KpnI G_GTAC'C Cuts at: 3596 3596 Size: 6271 MfeI C'AATT_G Cuts at: 3870 3870 Size: 6271 MluI A'CGCG_T Cuts at: 6147 6147 Size: 6271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3108 5385 5569 1305 Size: 1803 2277 184 2007 Fragments arranged by size: 2277 2007 1803 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4449 Fragments arranged by size: 4449 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2322 2555 2690 4229 4588 4747 518 Size: 648 1156 233 135 1539 359 159 2042 Fragments arranged by size: 2042 1539 1156 648 359 233 159 135 MspA1I CmG'CkG Cuts at: 1606 2173 2303 2627 3101 3687 4364 5512 5757 Size: 567 130 324 474 586 677 1148 245 Cuts at: 5757 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 586 567 474 324 245 130 NaeI GCC'GGC Cuts at: 2937 2937 Size: 6271 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4780 Fragments arranged by size: 4780 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6271 NgoMIV G'CCGG_C Cuts at: 2935 2935 Size: 6271 NheI G'CTAG_C Cuts at: 1052 2296 1052 Size: 1244 5027 NotI GC'GGCC_GC Cuts at: 3718 3718 Size: 6271 NspI r_CATG'y Cuts at: 1132 1229 2146 2323 2554 3271 3324 3629 5174 Size: 97 917 177 231 717 53 305 1545 Cuts at: 5174 1132 Size: 2229 Fragments arranged by size: 2229 1545 917 717 305 231 177 97 53 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6271 PciI A'CATG_T Cuts at: 2319 2550 5170 2319 Size: 231 2620 3420 Fragments arranged by size: 3420 2620 231 PflMI CCAn_nnn'nTGG Cuts at: 1108 3376 1108 Size: 2268 4003 PmeI GTTT'AAAC Cuts at: 3575 3575 Size: 6271 PmlI CAC'GTG Cuts at: 3237 3237 Size: 6271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2175 2207 3410 3442 4340 4372 5853 5885 2175 Size: 32 1203 32 898 32 1481 32 2561 Fragments arranged by size: 2561 1481 1203 898 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3442 3442 Size: 6271 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6271 PsiI TTA'TAA Cuts at: 3841 3841 Size: 6271 PstI C_TGCA'G Cuts at: 830 2654 3162 3623 830 Size: 1824 508 461 3478 Fragments arranged by size: 3478 1824 508 461 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6271 PvuII CAG'CTG Cuts at: 2173 2303 2627 2173 Size: 130 324 5817 Fragments arranged by size: 5817 324 130 SacII CC_GC'GG Cuts at: 3102 3102 Size: 6271 SalI G'TCGA_C Cuts at: 1936 3613 1936 Size: 1677 4594 SbfI CC_TGCA'GG Cuts at: 3623 3623 Size: 6271 ScaI AGT'ACT Cuts at: 3350 4519 6231 3350 Size: 1169 1712 3390 Fragments arranged by size: 3390 1712 1169 SexAI A'CCwGG_T Cuts at: 2808 2808 Size: 6271 SfcI C'TryA_G Cuts at: 826 1045 2036 2650 3158 3619 4754 5435 5626 Size: 219 991 614 508 461 1135 681 191 Cuts at: 5626 826 Size: 1471 Fragments arranged by size: 1471 1135 991 681 614 508 461 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6271 SmaI CCC'GGG Cuts at: 1872 3598 1872 Size: 1726 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2971 3278 3555 4375 5276 5538 Size: 197 926 1028 307 277 820 901 262 Cuts at: 5538 820 Size: 1553 Fragments arranged by size: 1553 1028 926 901 820 307 277 262 197 SnaBI TAC'GTA Cuts at: 493 4062 493 Size: 3569 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6271 SphI G_CATG'C Cuts at: 1132 3629 1132 Size: 2497 3774 SspI AAT'ATT Cuts at: 5 52 4195 5 Size: 47 4143 2081 Fragments arranged by size: 4143 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6271 StyI C'CwwG_G Cuts at: 513 1065 2004 3710 513 Size: 552 939 1706 3074 Fragments arranged by size: 3074 1706 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2956 3341 3484 4298 4315 4468 4653 Size: 1255 1376 385 143 814 17 153 185 Cuts at: 4653 5098 325 Size: 445 1498 Fragments arranged by size: 1498 1376 1255 814 445 385 185 153 143 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2125 3270 3348 Size: 274 80 33 51 1443 148 1145 78 Cuts at: 3348 3447 4517 6229 96 Size: 99 1070 1712 138 Fragments arranged by size: 1712 1443 1145 1070 274 148 138 99 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6231 6263 6231 Size: 32 6239 Tth111I GACn'n_nGTC Cuts at: 1316 2330 2423 2966 1316 Size: 1014 93 543 4621 Fragments arranged by size: 4621 1014 543 93 XbaI T'CTAG_A Cuts at: 3607 3607 Size: 6271 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6271 XmaI C'CCGG_G Cuts at: 1870 3596 1870 Size: 1726 4545 XmnI GAAnn'nnTTC Cuts at: 3257 4400 3257 Size: 1143 5128 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4079 276 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI