(Circular) (Six-base) MAPSORT of: pFN21AB4641.seq Check: 3368 from: 1 to: 7612 pFN21AB4641 7612 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5422 278 Size: 53 83 186 936 3886 2468 Fragments arranged by size: 3886 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4955 1937 Size: 3018 4594 Acc65I G'GTAC_C Cuts at: 4933 4933 Size: 7612 AclI AA'CG_TT Cuts at: 1563 3487 5739 6112 1563 Size: 1924 2252 373 3063 Fragments arranged by size: 3063 2252 1924 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3527 4278 5675 7059 7327 1632 Size: 1895 751 1397 1384 268 1917 Fragments arranged by size: 1917 1895 1397 1384 751 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7415 AflIII A'CryG_T Cuts at: 1203 3006 3197 4909 6511 7488 1203 Size: 1803 191 1712 1602 977 1327 Fragments arranged by size: 1803 1712 1602 1327 977 191 AgeI A'CCGG_T Cuts at: 6443 6443 Size: 7612 AhdI GACnn_n'nnGTC Cuts at: 6341 6341 Size: 7612 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7612 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7612 AlwNI CAG_nnn'CTG Cuts at: 4008 5355 6927 4008 Size: 1347 1572 4693 Fragments arranged by size: 4693 1572 1347 ApaLI G'TGCA_C Cuts at: 5669 6825 5669 Size: 1156 6456 ApoI r'AATT_y Cuts at: 1493 2555 2708 2768 2961 3341 3739 3970 4070 Size: 1062 153 60 193 380 398 231 100 Cuts at: 4070 4683 4726 4921 5147 5299 7408 1493 Size: 613 43 195 226 152 2109 1697 Fragments arranged by size: 2109 1697 1062 613 398 380 231 226 195 193 153 152 100 60 43 AseI AT'TA_AT Cuts at: 160 1032 6166 160 Size: 872 5134 1606 Fragments arranged by size: 5134 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7612 AvaI C'yCGr_G Cuts at: 1870 1943 2063 4937 1870 Size: 73 120 2874 4545 Fragments arranged by size: 4545 2874 120 73 AvrII C'CTAG_G Cuts at: 4771 4771 Size: 7612 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4011 4044 4011 Size: 33 7579 BamHI G'GATC_C Cuts at: 4942 5309 4942 Size: 367 7245 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3612 4775 4933 6388 618 Size: 325 217 627 1825 1163 158 1455 1842 Fragments arranged by size: 1842 1825 1455 1163 627 325 217 158 BanII G_rGCy'C Cuts at: 1391 2118 4320 4931 1391 Size: 727 2202 611 4072 Fragments arranged by size: 4072 2202 727 611 BbeI G_GCGC'C Cuts at: 3616 3616 Size: 7612 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3126 4892 928 Size: 971 1227 1766 3648 Fragments arranged by size: 3648 1766 1227 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4583 4617 5803 5837 1903 Size: 34 2646 34 1186 34 3678 Fragments arranged by size: 3678 2646 1186 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4012 5504 6714 4012 Size: 1492 1210 4910 Fragments arranged by size: 4910 1492 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7612 BfrBI ATG'CAT Cuts at: 3242 3780 4696 4700 3242 Size: 538 916 4 6154 Fragments arranged by size: 6154 916 538 4 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6223 136 Size: 107 122 71 631 605 4551 1525 Fragments arranged by size: 4551 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3169 7607 3169 Size: 4438 3174 BlpI GC'TnA_GC Cuts at: 5029 5029 Size: 7612 Bme1580I G_kGCm'C Cuts at: 1790 5673 6829 1790 Size: 3883 1156 2573 Fragments arranged by size: 3883 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7612 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4203 5343 6301 Size: 416 53 83 399 24 2786 1140 958 Cuts at: 6301 442 Size: 1753 Fragments arranged by size: 2786 1753 1140 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3623 1056 Size: 2567 5045 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2132 2432 2741 3468 6272 1239 Size: 806 87 300 309 727 2804 2579 Fragments arranged by size: 2804 2579 806 727 309 300 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2535 3255 4371 5737 6602 6900 2535 Size: 720 1116 1366 865 298 3247 Fragments arranged by size: 3247 1366 1116 865 720 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2084 6275 882 Size: 617 585 4191 2219 Fragments arranged by size: 4191 2219 617 585 BsaAI yAC'GTr Cuts at: 493 1204 3007 3076 3200 5403 493 Size: 711 1803 69 124 2203 2702 Fragments arranged by size: 2702 2203 1803 711 124 69 BsaBI GATnn'nnATC Cuts at: 4527 7606 4527 Size: 3079 4533 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3613 4785 5419 5801 Size: 53 83 186 936 2080 1172 634 382 Cuts at: 5801 6436 275 Size: 635 1451 Fragments arranged by size: 2080 1451 1172 936 635 634 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4447 6045 6443 6717 6864 7275 4447 Size: 1598 398 274 147 411 4784 Fragments arranged by size: 4784 1598 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7387 Fragments arranged by size: 7387 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2263 3054 3362 3743 4493 1186 Size: 895 182 791 308 381 750 4305 Fragments arranged by size: 4305 895 791 750 381 308 182 BseYI C'CCAG_C Cuts at: 2043 2389 6815 7311 2043 Size: 346 4426 496 2344 Fragments arranged by size: 4426 2344 496 346 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2155 1163 Size: 573 419 6620 Fragments arranged by size: 6620 573 419 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5062 5823 6851 1540 Size: 275 186 3061 761 1028 2301 Fragments arranged by size: 3061 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2085 4126 5673 5758 6829 2085 Size: 2041 1547 85 1071 2868 Fragments arranged by size: 2868 2041 1547 1071 85 BsmI GAATG_Cn' Cuts at: 2121 2842 4340 5123 5216 2121 Size: 721 1498 783 93 4517 Fragments arranged by size: 4517 1498 783 721 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2045 2388 2045 Size: 343 7269 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2118 4126 4320 4931 5673 5758 Size: 399 295 33 2008 194 611 742 85 Cuts at: 5758 6829 1391 Size: 1071 2174 Fragments arranged by size: 2174 2008 1071 742 611 399 295 194 85 33 BspEI T'CCGG_A Cuts at: 4447 7275 4447 Size: 2828 4784 BspHI T'CATG_A Cuts at: 5501 7231 5501 Size: 1730 5882 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3451 4380 4967 844 Size: 2607 929 587 3489 Fragments arranged by size: 3489 2607 929 587 BsrBI CCG'CTC Cuts at: 1394 1823 5499 1394 Size: 429 3676 3507 Fragments arranged by size: 3676 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3226 4543 4851 6107 6281 65 Size: 1183 1978 1317 308 1256 174 1396 Fragments arranged by size: 1978 1396 1317 1256 1183 308 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6256 6443 1700 Size: 110 4446 187 2869 Fragments arranged by size: 4446 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7612 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7612 BssSI C'ACGA_G Cuts at: 4264 5672 6684 4264 Size: 1408 1012 5192 Fragments arranged by size: 5192 1408 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2893 4838 794 Size: 2099 1945 3568 Fragments arranged by size: 3568 2099 1945 BstBI TT'CG_AA Cuts at: 6416 6416 Size: 7612 BstEII G'GTnAC_C Cuts at: 1184 3438 1184 Size: 2254 5358 BstYI r'GATC_y Cuts at: 1972 3082 3169 4516 4942 5309 5694 5711 7163 Size: 1110 87 1347 426 367 385 17 1452 Cuts at: 7163 7249 7261 7607 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1347 1110 426 385 367 346 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2012 4138 513 Size: 552 939 8 2126 3987 Fragments arranged by size: 3987 2126 939 552 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3052 3588 487 Size: 683 56 1826 536 4511 Fragments arranged by size: 4511 1826 683 536 56 BtsI GCAGTG_nn' Cuts at: 5128 5922 5942 5128 Size: 794 20 6798 Fragments arranged by size: 6798 794 20 ClaI AT'CG_AT Cuts at: 1554 2229 1554 Size: 675 6937 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2662 2697 529 Size: 35 2098 35 5444 Fragments arranged by size: 5444 2098 35 35 DraI TTT'AAA Cuts at: 4888 4916 4888 Size: 28 7584 DraIII CAC_nnn'GTG Cuts at: 3770 3770 Size: 7612 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6619 809 Size: 507 5303 1802 Fragments arranged by size: 5303 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3058 4010 5059 Size: 54 1425 50 275 18 1228 952 1049 Cuts at: 5059 5948 7577 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1228 1049 952 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5059 1537 Size: 275 3247 4090 Fragments arranged by size: 4090 3247 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3408 3720 4053 5542 1353 Size: 2055 312 333 1489 3423 Fragments arranged by size: 3423 2055 1489 333 312 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2063 4081 6199 6573 6719 387 Size: 1064 138 474 2018 2118 374 146 1280 Fragments arranged by size: 2118 2018 1280 1064 474 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2132 2432 2741 3468 3527 4278 Size: 393 413 87 300 309 727 59 751 Cuts at: 4278 5675 6272 7059 7327 1239 Size: 1397 597 787 268 1524 Fragments arranged by size: 1524 1397 787 751 727 597 413 393 309 300 268 87 59 EcoNI CCTnn'n_nnAGG Cuts at: 3467 3467 Size: 7612 EcoO109I rG'GnC_Cy Cuts at: 3000 4144 4369 3000 Size: 1144 225 6243 Fragments arranged by size: 6243 1144 225 EcoRI G'AATT_C Cuts at: 4921 5299 4921 Size: 378 7234 EcoRV GAT'ATC Cuts at: 4563 4563 Size: 7612 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3616 3616 Size: 7612 FspI TGC'GCA Cuts at: 2621 6118 2621 Size: 3497 4115 HaeII r_GCGC'y Cuts at: 1678 2044 3616 6759 1678 Size: 366 1572 3143 2531 Fragments arranged by size: 3143 2531 1572 366 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2089 2385 2417 2579 Size: 32 135 32 485 32 296 32 162 Cuts at: 2579 2611 2747 2779 3074 3106 3984 4016 4552 Size: 32 136 32 295 32 878 32 536 Cuts at: 4552 4584 6253 6285 6327 6359 1373 Size: 32 1669 32 42 32 2626 Fragments arranged by size: 2626 1669 878 536 485 296 295 162 136 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2305 2751 4956 5202 669 Size: 988 281 367 446 2205 246 3079 Fragments arranged by size: 3079 2205 988 446 367 281 246 HindIII A'AGCT_T Cuts at: 748 2696 748 Size: 1948 5664 HpaI GTT'AAC Cuts at: 2751 5202 2751 Size: 2451 5161 KasI G'GCGC_C Cuts at: 3612 3612 Size: 7612 KpnI G_GTAC'C Cuts at: 4937 4937 Size: 7612 MfeI C'AATT_G Cuts at: 4734 4827 5211 4734 Size: 93 384 7135 Fragments arranged by size: 7135 384 93 MluI A'CGCG_T Cuts at: 7488 7488 Size: 7612 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6726 6910 1305 Size: 5421 184 2007 Fragments arranged by size: 5421 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3060 4012 10 Size: 54 1425 343 1228 952 3610 Fragments arranged by size: 3610 1425 1228 952 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2994 5570 5929 6088 518 Size: 648 1828 2576 359 159 2042 Fragments arranged by size: 2576 2042 1828 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2023 3634 5028 5705 6853 7098 1606 Size: 408 9 1611 1394 677 1148 245 2120 Fragments arranged by size: 2120 1611 1394 1148 677 408 245 9 NarI GG'CG_CC Cuts at: 3613 3613 Size: 7612 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6121 Fragments arranged by size: 6121 939 552 NdeI CA'TA_TG Cuts at: 387 4202 387 Size: 3815 3797 NheI G'CTAG_C Cuts at: 1052 3619 1052 Size: 2567 5045 NotI GC'GGCC_GC Cuts at: 5059 5059 Size: 7612 NsiI A_TGCA'T Cuts at: 3244 3782 4698 4702 3244 Size: 538 916 4 6154 Fragments arranged by size: 6154 916 538 4 NspI r_CATG'y Cuts at: 1132 1229 2389 4700 4913 4970 6515 1132 Size: 97 1160 2311 213 57 1545 2229 Fragments arranged by size: 2311 2229 1545 1160 213 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7612 PciI A'CATG_T Cuts at: 4909 6511 4909 Size: 1602 6010 PflMI CCAn_nnn'nTGG Cuts at: 1108 3092 4215 1108 Size: 1984 1123 4505 Fragments arranged by size: 4505 1984 1123 PfoI T'CCnGG_A Cuts at: 3978 3978 Size: 7612 PmeI GTTT'AAAC Cuts at: 4916 4916 Size: 7612 PmlI CAC'GTG Cuts at: 3076 3076 Size: 7612 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5681 5713 7194 7226 5681 Size: 32 1481 32 6067 Fragments arranged by size: 6067 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3000 4369 3000 Size: 1369 6243 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7612 PsiI TTA'TAA Cuts at: 3531 5182 3531 Size: 1651 5961 PstI C_TGCA'G Cuts at: 830 2022 3834 4964 830 Size: 1192 1812 1130 3478 Fragments arranged by size: 3478 1812 1192 1130 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7612 PvuII CAG'CTG Cuts at: 2023 3634 2023 Size: 1611 6001 SacII CC_GC'GG Cuts at: 2015 2015 Size: 7612 SalI G'TCGA_C Cuts at: 1936 4954 1936 Size: 3018 4594 SbfI CC_TGCA'GG Cuts at: 4964 4964 Size: 7612 ScaI AGT'ACT Cuts at: 2453 3839 5860 7572 2453 Size: 1386 2021 1712 2493 Fragments arranged by size: 2493 2021 1712 1386 SfcI C'TryA_G Cuts at: 826 1045 2018 3830 4960 6095 6776 6967 826 Size: 219 973 1812 1130 1135 681 191 1471 Fragments arranged by size: 1812 1471 1135 1130 973 681 219 191 SfoI GGC'GCC Cuts at: 3614 3614 Size: 7612 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7612 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7612 SmaI CCC'GGG Cuts at: 1872 4939 1872 Size: 3067 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2550 3270 4386 5716 6617 6879 Size: 197 926 607 720 1116 1330 901 262 Cuts at: 6879 820 Size: 1553 Fragments arranged by size: 1553 1330 1116 926 901 720 607 262 197 SnaBI TAC'GTA Cuts at: 493 5403 493 Size: 4910 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7612 SphI G_CATG'C Cuts at: 1132 4700 4970 1132 Size: 3568 270 3774 Fragments arranged by size: 3774 3568 270 SspI AAT'ATT Cuts at: 5 52 2626 5536 5 Size: 47 2574 2910 2081 Fragments arranged by size: 2910 2574 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7612 StyI C'CwwG_G Cuts at: 513 1065 2004 2284 2517 2734 4771 4780 5051 Size: 552 939 280 233 217 2037 9 271 Cuts at: 5051 513 Size: 3074 Fragments arranged by size: 3074 2037 939 552 280 271 233 217 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3574 5639 5656 5809 5994 6439 325 Size: 1255 1994 2065 17 153 185 445 1498 Fragments arranged by size: 2065 1994 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2451 3837 5858 Size: 274 80 33 51 1443 474 1386 2021 Cuts at: 5858 7570 96 Size: 1712 138 Fragments arranged by size: 2021 1712 1443 1386 474 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3570 3602 7572 7604 3570 Size: 32 3970 32 3578 Fragments arranged by size: 3970 3578 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3996 1316 Size: 2680 4932 XbaI T'CTAG_A Cuts at: 4948 4948 Size: 7612 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7612 XmaI C'CCGG_G Cuts at: 1870 4937 1870 Size: 3067 4545 XmnI GAAnn'nnTTC Cuts at: 3753 5741 3753 Size: 1988 5624 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5420 276 Size: 53 83 186 936 3886 2468 Fragments arranged by size: 3886 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI