Restriction map for
pFN21AB4641
(Linear) (Six-base) MAPSORT of: KIBB4641.seq Check: 863 from: 1 to: 2907
KIBB4641.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AclI AA'CG_TT
Cuts at: 0 1482 2907
Size: 1482 1425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1522 2273 2907
Size: 1522 751 634
Fragments arranged by size:
1522 751 634
AflIII A'CryG_T
Cuts at: 0 1001 1192 2907
Size: 1001 191 1715
Fragments arranged by size:
1715 1001 191
AlwNI CAG_nnn'CTG
Cuts at: 0 2003 2907
Size: 2003 904
ApoI r'AATT_y
Cuts at: 0 550 703 763 956 1336 1734 1965 2065
Size: 550 153 60 193 380 398 231 100
Cuts at: 2065 2678 2721 2907
Size: 613 43 186
Fragments arranged by size:
613 550 398 380 231 193 186 153
100 60 43
AvaI C'yCGr_G
Cuts at: 0 58 2907
Size: 58 2849
AvrII C'CTAG_G
Cuts at: 0 2766 2907
Size: 2766 141
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2006 2039 2907
Size: 2006 33 868
Fragments arranged by size:
2006 868 33
BanI G'GyrC_C
Cuts at: 0 1607 2770 2907
Size: 1607 1163 137
Fragments arranged by size:
1607 1163 137
BanII G_rGCy'C
Cuts at: 0 113 2315 2907
Size: 113 2202 592
Fragments arranged by size:
2202 592 113
BbeI G_GCGC'C
Cuts at: 0 1611 2907
Size: 1611 1296
BbsI GAAGACnn'nnnn_
Cuts at: 0 1121 2887 2907
Size: 1121 1766 20
Fragments arranged by size:
1766 1121 20
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2578 2612 2907
Size: 2578 34 295
Fragments arranged by size:
2578 295 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2007 2907
Size: 2007 900
BfrBI ATG'CAT
Cuts at: 0 1237 1775 2691 2695 2907
Size: 1237 538 916 4 212
Fragments arranged by size:
1237 916 538 212 4
BglII A'GATC_T
Cuts at: 0 1164 2907
Size: 1164 1743
BmrI ACTGGGnnnn_n'
Cuts at: 0 2198 2907
Size: 2198 709
BmtI G_CTAG'C
Cuts at: 0 1618 2907
Size: 1618 1289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 127 427 736 1463 2907
Size: 40 87 300 309 727 1444
Fragments arranged by size:
1444 727 309 300 87 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 1250 2366 2907
Size: 530 720 1116 541
Fragments arranged by size:
1116 720 541 530
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 2907
Size: 79 2828
BsaAI yAC'GTr
Cuts at: 0 1002 1071 1195 2907
Size: 1002 69 124 1712
Fragments arranged by size:
1712 1002 124 69
BsaBI GATnn'nnATC
Cuts at: 0 2522 2907
Size: 2522 385
BsaHI Gr'CG_yC
Cuts at: 0 1608 2780 2907
Size: 1608 1172 127
Fragments arranged by size:
1608 1172 127
BsaWI w'CCGG_w
Cuts at: 0 2442 2907
Size: 2442 465
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 258 1049 1357 1738 2488 2907
Size: 76 182 791 308 381 750 419
Fragments arranged by size:
791 750 419 381 308 182 76
BseYI C'CCAG_C
Cuts at: 0 38 384 2907
Size: 38 346 2523
Fragments arranged by size:
2523 346 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 2907
Size: 150 2757
BsiHKAI G_wGCw'C
Cuts at: 0 80 2121 2907
Size: 80 2041 786
Fragments arranged by size:
2041 786 80
BsmI GAATG_Cn'
Cuts at: 0 116 837 2335 2907
Size: 116 721 1498 572
Fragments arranged by size:
1498 721 572 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 40 383 2907
Size: 40 343 2524
Fragments arranged by size:
2524 343 40
Bsp1286I G_dGCh'C
Cuts at: 0 80 113 2121 2315 2907
Size: 80 33 2008 194 592
Fragments arranged by size:
2008 592 194 80 33
BspEI T'CCGG_A
Cuts at: 0 2442 2907
Size: 2442 465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1446 2375 2907
Size: 1446 929 532
Fragments arranged by size:
1446 929 532
BsrDI GCAATG_nn'
Cuts at: 0 1221 2538 2846 2907
Size: 1221 1317 308 61
Fragments arranged by size:
1317 1221 308 61
BssSI C'ACGA_G
Cuts at: 0 2259 2907
Size: 2259 648
BstAPI GCAn_nnn'nTGC
Cuts at: 0 888 2833 2907
Size: 888 1945 74
Fragments arranged by size:
1945 888 74
BstEII G'GTnAC_C
Cuts at: 0 1433 2907
Size: 1433 1474
BstYI r'GATC_y
Cuts at: 0 1077 1164 2511 2907
Size: 1077 87 1347 396
Fragments arranged by size:
1347 1077 396 87
BtgI C'CryG_G
Cuts at: 0 7 2133 2907
Size: 7 2126 774
Fragments arranged by size:
2126 774 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1047 1583 2907
Size: 1047 536 1324
Fragments arranged by size:
1324 1047 536
ClaI AT'CG_AT
Cuts at: 0 224 2907
Size: 224 2683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 657 692 2907
Size: 657 35 2215
Fragments arranged by size:
2215 657 35
DraI TTT'AAA
Cuts at: 0 2883 2907
Size: 2883 24
DraIII CAC_nnn'GTG
Cuts at: 0 1765 2907
Size: 1765 1142
EaeI y'GGCC_r
Cuts at: 0 1053 2005 2907
Size: 1053 952 902
Fragments arranged by size:
1053 952 902
EarI CTCTTCn'nnn_
Cuts at: 0 1403 1715 2048 2907
Size: 1403 312 333 859
Fragments arranged by size:
1403 859 333 312
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 58 2076 2907
Size: 58 2018 831
Fragments arranged by size:
2018 831 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 127 427 736 1463 1522 2273 2907
Size: 40 87 300 309 727 59 751 634
Fragments arranged by size:
751 727 634 309 300 87 59 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1462 2907
Size: 1462 1445
EcoO109I rG'GnC_Cy
Cuts at: 0 995 2139 2364 2907
Size: 995 1144 225 543
Fragments arranged by size:
1144 995 543 225
EcoRV GAT'ATC
Cuts at: 0 2558 2907
Size: 2558 349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1611 2907
Size: 1611 1296
FspI TGC'GCA
Cuts at: 0 616 2907
Size: 616 2291
HaeII r_GCGC'y
Cuts at: 0 39 1611 2907
Size: 39 1572 1296
Fragments arranged by size:
1572 1296 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 380 412 574 606 742 774
Size: 52 32 296 32 162 32 136 32
Cuts at: 774 1069 1101 1979 2011 2547 2579 2907
Size: 295 32 878 32 536 32 328
Fragments arranged by size:
878 536 328 296 295 162 136 52
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 300 746 2907
Size: 300 446 2161
Fragments arranged by size:
2161 446 300
HindIII A'AGCT_T
Cuts at: 0 691 2907
Size: 691 2216
HpaI GTT'AAC
Cuts at: 0 746 2907
Size: 746 2161
KasI G'GCGC_C
Cuts at: 0 1607 2907
Size: 1607 1300
MfeI C'AATT_G
Cuts at: 0 2729 2822 2907
Size: 2729 93 85
Fragments arranged by size:
2729 93 85
MscI TGG'CCA
Cuts at: 0 1055 2007 2907
Size: 1055 952 900
Fragments arranged by size:
1055 952 900
MslI CAynn'nnrTG
Cuts at: 0 989 2907
Size: 989 1918
MspA1I CmG'CkG
Cuts at: 0 9 18 1629 2907
Size: 9 9 1611 1278
Fragments arranged by size:
1611 1278 9 9
NarI GG'CG_CC
Cuts at: 0 1608 2907
Size: 1608 1299
NdeI CA'TA_TG
Cuts at: 0 2197 2907
Size: 2197 710
NheI G'CTAG_C
Cuts at: 0 1614 2907
Size: 1614 1293
NsiI A_TGCA'T
Cuts at: 0 1239 1777 2693 2697 2907
Size: 1239 538 916 4 210
Fragments arranged by size:
1239 916 538 210 4
NspI r_CATG'y
Cuts at: 0 384 2695 2907
Size: 384 2311 212
Fragments arranged by size:
2311 384 212
PflMI CCAn_nnn'nTGG
Cuts at: 0 1087 2210 2907
Size: 1087 1123 697
Fragments arranged by size:
1123 1087 697
PfoI T'CCnGG_A
Cuts at: 0 1973 2907
Size: 1973 934
PmlI CAC'GTG
Cuts at: 0 1071 2907
Size: 1071 1836
PpuMI rG'GwC_Cy
Cuts at: 0 995 2364 2907
Size: 995 1369 543
Fragments arranged by size:
1369 995 543
PsiI TTA'TAA
Cuts at: 0 1526 2907
Size: 1526 1381
PstI C_TGCA'G
Cuts at: 0 17 1829 2907
Size: 17 1812 1078
Fragments arranged by size:
1812 1078 17
PvuII CAG'CTG
Cuts at: 0 18 1629 2907
Size: 18 1611 1278
Fragments arranged by size:
1611 1278 18
SacII CC_GC'GG
Cuts at: 0 10 2907
Size: 10 2897
ScaI AGT'ACT
Cuts at: 0 448 1834 2907
Size: 448 1386 1073
Fragments arranged by size:
1386 1073 448
SfcI C'TryA_G
Cuts at: 0 13 1825 2907
Size: 13 1812 1082
Fragments arranged by size:
1812 1082 13
SfoI GGC'GCC
Cuts at: 0 1609 2907
Size: 1609 1298
SmlI C'TyrA_G
Cuts at: 0 545 1265 2381 2907
Size: 545 720 1116 526
Fragments arranged by size:
1116 720 545 526
SphI G_CATG'C
Cuts at: 0 2695 2907
Size: 2695 212
SspI AAT'ATT
Cuts at: 0 621 2907
Size: 621 2286
StyI C'CwwG_G
Cuts at: 0 279 512 729 2766 2775 2907
Size: 279 233 217 2037 9 132
Fragments arranged by size:
2037 279 233 217 132 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1569 2907
Size: 1569 1338
TatI w'GTAC_w
Cuts at: 0 446 1832 2907
Size: 446 1386 1075
Fragments arranged by size:
1386 1075 446
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1565 1597 2907
Size: 1565 32 1310
Fragments arranged by size:
1565 1310 32
Tth111I GACn'n_nGTC
Cuts at: 0 1991 2907
Size: 1991 916
XmnI GAAnn'nnTTC
Cuts at: 0 1748 2907
Size: 1748 1159
Enzymes that do cut:
AclI AcuI AflIII AlwNI ApoI AvaI AvrII BaeI
BanI BanII BbeI BbsI BcgI BciVI BfrBI BglII
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrDI BssSI BstAPI BstEII BstYI BtgI
BtgZI ClaI CspCI DraI DraIII EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI MfeI MscI MslI MspA1I
NarI NdeI NheI NsiI NspI PflMI PfoI PmlI
PpuMI PsiI PstI PvuII SacII ScaI SfcI SfoI
SmlI SphI SspI StyI TaqII TatI TstI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
BamHI BbvCI BclI BglI BlpI Bme1580I BmgBI BplI
Bpu10I BsaXI BsiEI BsiWI BspHI BsrBI BsrFI BsrGI
BssHII BstBI BstXI BstZ17I Bsu36I BtsI DrdI EagI
EcoICRI EcoRI FseI FspAI KpnI MluI MmeI NaeI
NcoI NgoMIV NotI NruI PacI PasI PciI PmeI
PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI XbaI XcmI
XhoI XmaI ZraI