(Circular) (Six-base) MAPSORT of: pFN21AB4594.seq Check: 5315 from: 1 to: 6823 pFN21AB4594 6823 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3247 3957 3247 Size: 710 6113 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4633 278 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4166 1937 Size: 2229 4594 Acc65I G'GTAC_C Cuts at: 4144 4144 Size: 6823 AclI AA'CG_TT Cuts at: 1563 4950 5323 1563 Size: 3387 373 3063 Fragments arranged by size: 3387 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3914 3959 4886 6270 6538 1632 Size: 2282 45 927 1384 268 1917 Fragments arranged by size: 2282 1917 1384 927 268 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6626 AflIII A'CryG_T Cuts at: 1203 5722 6699 1203 Size: 4519 977 1327 Fragments arranged by size: 4519 1327 977 AgeI A'CCGG_T Cuts at: 3710 5654 3710 Size: 1944 4879 AhdI GACnn_n'nnGTC Cuts at: 3280 5552 3280 Size: 2272 4551 AleI CACnn'nnGTG Cuts at: 1166 2829 3855 3879 4067 1166 Size: 1663 1026 24 188 3922 Fragments arranged by size: 3922 1663 1026 188 24 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3484 1184 Size: 2300 4523 AlwNI CAG_nnn'CTG Cuts at: 2720 3937 4091 4566 6138 2720 Size: 1217 154 475 1572 3405 Fragments arranged by size: 3405 1572 1217 475 154 ApaI G_GGCC'C Cuts at: 3748 3748 Size: 6823 ApaLI G'TGCA_C Cuts at: 3427 4880 6036 3427 Size: 1453 1156 4214 Fragments arranged by size: 4214 1453 1156 ApoI r'AATT_y Cuts at: 1493 3786 4132 4358 4510 6619 1493 Size: 2293 346 226 152 2109 1697 Fragments arranged by size: 2293 2109 1697 346 226 152 AseI AT'TA_AT Cuts at: 160 1032 5377 160 Size: 872 4345 1606 Fragments arranged by size: 4345 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 AvaI C'yCGr_G Cuts at: 1870 1943 4148 1870 Size: 73 2205 4545 Fragments arranged by size: 4545 2205 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2011 2044 2011 Size: 33 6790 BamHI G'GATC_C Cuts at: 4153 4520 4153 Size: 367 6456 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3911 3924 4049 4144 5599 Size: 325 217 627 2124 13 125 95 1455 Cuts at: 5599 618 Size: 1842 Fragments arranged by size: 2124 1842 1455 627 325 217 125 95 13 BanII G_rGCy'C Cuts at: 1391 2140 2958 3005 3104 3374 3748 4110 4142 Size: 749 818 47 99 270 374 362 32 Cuts at: 4142 1391 Size: 4072 Fragments arranged by size: 4072 818 749 374 362 270 99 47 32 BbeI G_GCGC'C Cuts at: 4053 4053 Size: 6823 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5852 BbvCI CC'TCA_GC Cuts at: 2838 2863 2838 Size: 25 6798 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5014 5048 1903 Size: 34 3077 34 3678 Fragments arranged by size: 3678 3077 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2039 4715 5925 2039 Size: 2676 1210 2937 Fragments arranged by size: 2937 2676 1210 BclI T'GATC_A Cuts at: 1548 2217 1548 Size: 669 6154 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 3230 5434 Size: 107 122 71 631 605 343 1215 2204 Cuts at: 5434 136 Size: 1525 Fragments arranged by size: 2204 1525 1215 631 605 343 122 107 71 BglII A'GATC_T Cuts at: 6818 6818 Size: 6823 BlpI GC'TnA_GC Cuts at: 3198 4240 3198 Size: 1042 5781 Bme1580I G_kGCm'C Cuts at: 1790 2692 3431 3748 3862 4884 6040 1790 Size: 902 739 317 114 1022 1156 2573 Fragments arranged by size: 2573 1156 1022 902 739 317 114 BmgBI CAC'GTC Cuts at: 1318 3432 1318 Size: 2114 4709 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3511 3742 4554 Size: 416 53 83 399 24 2094 231 812 Cuts at: 4554 5512 442 Size: 958 1753 Fragments arranged by size: 2094 1753 958 812 416 399 231 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6823 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2938 3415 3613 2938 Size: 477 198 6148 Fragments arranged by size: 6148 477 198 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3056 3075 3263 3816 5483 1239 Size: 1817 19 188 553 1667 2579 Fragments arranged by size: 2579 1817 1667 553 188 19 Bpu10I CC'TnA_GC Cuts at: 2838 2863 3189 2838 Size: 25 326 6472 Fragments arranged by size: 6472 326 25 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3587 4948 5813 6111 3587 Size: 1361 865 298 4299 Fragments arranged by size: 4299 1361 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2911 3506 5486 882 Size: 617 1412 595 1980 2219 Fragments arranged by size: 2219 1980 1412 617 595 BsaAI yAC'GTr Cuts at: 493 1204 4614 493 Size: 711 3410 2702 Fragments arranged by size: 3410 2702 711 BsaBI GATnn'nnATC Cuts at: 6817 6817 Size: 6823 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4050 4630 5012 5647 Size: 53 83 186 936 2517 580 382 635 Cuts at: 5647 275 Size: 1451 Fragments arranged by size: 2517 1451 936 635 580 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2079 2652 2976 3710 4102 5256 5654 5928 6075 Size: 573 324 734 392 1154 398 274 147 Cuts at: 6075 6486 2079 Size: 411 2416 Fragments arranged by size: 2416 1154 734 573 411 398 392 324 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3358 3388 1178 Size: 30 165 30 1955 30 4613 Fragments arranged by size: 4613 1955 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2409 2527 2612 2651 2914 3515 3605 1186 Size: 1223 118 85 39 263 601 90 4404 Fragments arranged by size: 4404 1223 601 263 118 90 85 39 BseYI C'CCAG_C Cuts at: 2549 3202 6026 6522 2549 Size: 653 2824 496 2850 Fragments arranged by size: 2850 2824 653 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6250 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2738 4273 5034 6062 1540 Size: 275 186 737 1535 761 1028 2301 Fragments arranged by size: 2301 1535 1028 761 737 275 186 BsiHKAI G_wGCw'C Cuts at: 3153 3431 4110 4884 4969 6040 3153 Size: 278 679 774 85 1071 3936 Fragments arranged by size: 3936 1071 774 679 278 85 BsmI GAATG_Cn' Cuts at: 3064 3175 4334 4427 3064 Size: 111 1159 93 5460 Fragments arranged by size: 5460 1159 111 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2081 2081 Size: 6823 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2692 2958 3005 3104 3153 3374 Size: 399 350 552 266 47 99 49 221 Cuts at: 3374 3431 3748 3862 4110 4142 4884 4969 6040 Size: 57 317 114 248 32 742 85 1071 Cuts at: 6040 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 552 399 350 317 266 248 221 114 99 85 57 49 47 32 BspEI T'CCGG_A Cuts at: 2079 2652 4102 6486 2079 Size: 573 1450 2384 2416 Fragments arranged by size: 2416 2384 1450 573 BspHI T'CATG_A Cuts at: 4712 6442 4712 Size: 1730 5093 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 2511 2860 3247 3830 3957 4005 4104 Size: 1502 165 349 387 583 127 48 99 Cuts at: 4104 4178 844 Size: 74 3489 Fragments arranged by size: 3489 1502 583 387 349 165 127 99 74 48 BsrBI CCG'CTC Cuts at: 1394 1823 2686 4710 1394 Size: 429 863 2024 3507 Fragments arranged by size: 3507 2024 863 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3571 5318 5492 65 Size: 1183 2323 1747 174 1396 Fragments arranged by size: 2323 1747 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2733 3336 3710 3756 5467 5654 Size: 110 204 719 603 374 46 1711 187 Cuts at: 5654 1700 Size: 2869 Fragments arranged by size: 2869 1711 719 603 374 204 187 110 46 BsrGI T'GTAC_A Cuts at: 96 2274 2556 2595 3838 96 Size: 2178 282 39 1243 3081 Fragments arranged by size: 3081 2178 1243 282 39 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6823 BssSI C'ACGA_G Cuts at: 2421 3219 4883 5895 2421 Size: 798 1664 1012 3349 Fragments arranged by size: 3349 1664 1012 798 BstAPI GCAn_nnn'nTGC Cuts at: 794 3435 3616 794 Size: 2641 181 4001 Fragments arranged by size: 4001 2641 181 BstBI TT'CG_AA Cuts at: 5627 5627 Size: 6823 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6823 BstXI CCAn_nnnn'nTGG Cuts at: 3080 3482 4046 3080 Size: 402 564 5857 Fragments arranged by size: 5857 564 402 BstYI r'GATC_y Cuts at: 1972 2227 4153 4520 4905 4922 6374 6460 6472 Size: 255 1926 367 385 17 1452 86 12 Cuts at: 6472 6818 1972 Size: 346 1977 Fragments arranged by size: 1977 1926 1452 385 367 346 255 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2244 3498 2244 Size: 1254 5569 BtgI C'CryG_G Cuts at: 513 1065 2004 2325 3255 513 Size: 552 939 321 930 4081 Fragments arranged by size: 4081 939 930 552 321 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6084 Fragments arranged by size: 6084 683 56 BtsI GCAGTG_nn' Cuts at: 2170 4339 5133 5153 2170 Size: 2169 794 20 3840 Fragments arranged by size: 3840 2169 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6823 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3721 3756 529 Size: 35 3157 35 3596 Fragments arranged by size: 3596 3157 35 35 DraI TTT'AAA Cuts at: 4127 4127 Size: 6823 DraIII CAC_nnn'GTG Cuts at: 2816 2816 Size: 6823 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5830 809 Size: 507 4514 1802 Fragments arranged by size: 4514 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 2664 2735 Size: 54 1425 50 275 18 492 342 71 Cuts at: 2735 3758 4270 5159 6788 8 Size: 1023 512 889 1629 43 Fragments arranged by size: 1629 1425 1023 889 512 492 342 275 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2735 4270 1537 Size: 275 923 1535 4090 Fragments arranged by size: 4090 1535 923 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2421 2630 2799 2907 3935 4753 1353 Size: 1068 209 169 108 1028 818 3423 Fragments arranged by size: 3423 1068 1028 818 209 169 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5410 5784 5930 387 Size: 1064 138 3821 374 146 1280 Fragments arranged by size: 3821 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4108 4108 Size: 6823 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3056 3075 3263 3816 3914 3959 4886 Size: 393 1424 19 188 553 98 45 927 Cuts at: 4886 5483 6270 6538 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1424 927 787 597 553 393 268 188 98 45 19 EcoO109I rG'GnC_Cy Cuts at: 2842 3185 3552 3744 2842 Size: 343 367 192 5921 Fragments arranged by size: 5921 367 343 192 EcoRI G'AATT_C Cuts at: 3786 4132 4510 3786 Size: 346 378 6099 Fragments arranged by size: 6099 378 346 FspI TGC'GCA Cuts at: 5329 5329 Size: 6823 HaeII r_GCGC'y Cuts at: 1678 2520 4053 5970 1678 Size: 842 1533 1917 2531 Fragments arranged by size: 2531 1917 1533 842 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2241 2273 3266 3298 3358 Size: 32 135 32 669 32 993 32 60 Cuts at: 3358 3390 3503 3535 5464 5496 5538 5570 1373 Size: 32 113 32 1929 32 42 32 2626 Fragments arranged by size: 2626 1929 993 669 135 113 60 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3355 4167 4413 669 Size: 988 281 1417 812 246 3079 Fragments arranged by size: 3079 1417 988 812 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6823 HpaI GTT'AAC Cuts at: 3355 4413 3355 Size: 1058 5765 KasI G'GCGC_C Cuts at: 4049 4049 Size: 6823 KpnI G_GTAC'C Cuts at: 4148 4148 Size: 6823 MfeI C'AATT_G Cuts at: 4422 4422 Size: 6823 MluI A'CGCG_T Cuts at: 6699 6699 Size: 6823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5937 6121 1305 Size: 4632 184 2007 Fragments arranged by size: 4632 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2324 2666 10 Size: 54 1425 343 492 342 4167 Fragments arranged by size: 4167 1425 492 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2049 2114 2315 2330 2695 2829 3711 Size: 648 883 65 201 15 365 134 882 Cuts at: 3711 3855 3879 4067 4781 5140 5299 518 Size: 144 24 188 714 359 159 2042 Fragments arranged by size: 2042 883 882 714 648 365 359 201 188 159 144 134 65 24 15 MspA1I CmG'CkG Cuts at: 1606 2025 3306 3482 3654 4021 4239 4916 6064 Size: 419 1281 176 172 367 218 677 1148 Cuts at: 6064 6309 1606 Size: 245 2120 Fragments arranged by size: 2120 1281 1148 677 419 367 245 218 176 172 NaeI GCC'GGC Cuts at: 2016 2735 3338 2016 Size: 719 603 5501 Fragments arranged by size: 5501 719 603 NarI GG'CG_CC Cuts at: 4050 4050 Size: 6823 NcoI C'CATG_G Cuts at: 513 1065 2004 3255 513 Size: 552 939 1251 4081 Fragments arranged by size: 4081 1251 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6823 NgoMIV G'CCGG_C Cuts at: 2014 2733 3336 2014 Size: 719 603 5501 Fragments arranged by size: 5501 719 603 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6823 NotI GC'GGCC_GC Cuts at: 4270 4270 Size: 6823 NspI r_CATG'y Cuts at: 1132 1229 2354 3415 3461 4181 5726 1132 Size: 97 1125 1061 46 720 1545 2229 Fragments arranged by size: 2229 1545 1125 1061 720 97 46 PasI CC'CwG_GG Cuts at: 1348 2522 1348 Size: 1174 5649 PciI A'CATG_T Cuts at: 5722 5722 Size: 6823 PflMI CCAn_nnn'nTGG Cuts at: 1108 2720 1108 Size: 1612 5211 PfoI T'CCnGG_A Cuts at: 2262 2262 Size: 6823 PmeI GTTT'AAAC Cuts at: 4127 4127 Size: 6823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4892 4924 6405 6437 4892 Size: 32 1481 32 5278 Fragments arranged by size: 5278 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6823 PsiI TTA'TAA Cuts at: 4393 4393 Size: 6823 PspOMI G'GGCC_C Cuts at: 3744 3744 Size: 6823 PstI C_TGCA'G Cuts at: 830 2165 2343 3050 3621 4175 830 Size: 1335 178 707 571 554 3478 Fragments arranged by size: 3478 1335 707 571 554 178 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6823 PvuII CAG'CTG Cuts at: 2025 4021 2025 Size: 1996 4827 SacI G_AGCT'C Cuts at: 4110 4110 Size: 6823 SalI G'TCGA_C Cuts at: 1936 4165 1936 Size: 2229 4594 SapI GCTCTTCn'nnn_ Cuts at: 3935 3935 Size: 6823 SbfI CC_TGCA'GG Cuts at: 4175 4175 Size: 6823 ScaI AGT'ACT Cuts at: 3527 5071 6783 3527 Size: 1544 1712 3567 Fragments arranged by size: 3567 1712 1544 SfcI C'TryA_G Cuts at: 826 1045 2161 2176 2339 2827 3046 3617 4171 Size: 219 1116 15 163 488 219 571 554 Cuts at: 4171 5306 5987 6178 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1116 681 571 554 488 219 219 191 163 15 SfoI GGC'GCC Cuts at: 4051 4051 Size: 6823 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6823 SmaI CCC'GGG Cuts at: 1872 4150 1872 Size: 2278 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3602 4927 5828 6090 820 Size: 197 926 1659 1325 901 262 1553 Fragments arranged by size: 1659 1553 1325 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4614 493 Size: 4121 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6823 SphI G_CATG'C Cuts at: 1132 3461 4181 1132 Size: 2329 720 3774 Fragments arranged by size: 3774 2329 720 SspI AAT'ATT Cuts at: 5 52 4747 5 Size: 47 4695 2081 Fragments arranged by size: 4695 2081 47 StuI AGG'CCT Cuts at: 1517 3596 3662 1517 Size: 2079 66 4678 Fragments arranged by size: 4678 2079 66 StyI C'CwwG_G Cuts at: 513 1065 2004 2679 2787 3255 4065 4262 513 Size: 552 939 675 108 468 810 197 3074 Fragments arranged by size: 3074 939 810 675 552 468 197 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2514 2800 3268 4850 4867 5020 5205 Size: 1255 934 286 468 1582 17 153 185 Cuts at: 5205 5650 325 Size: 445 1498 Fragments arranged by size: 1582 1498 1255 934 468 445 286 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2274 2556 2595 Size: 274 80 33 51 1443 297 282 39 Cuts at: 2595 2727 3525 3838 5069 6781 96 Size: 132 798 313 1231 1712 138 Fragments arranged by size: 1712 1443 1231 798 313 297 282 274 138 132 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6783 6815 6783 Size: 32 6791 Tth111I GACn'n_nGTC Cuts at: 1316 3517 1316 Size: 2201 4622 XbaI T'CTAG_A Cuts at: 2224 4159 2224 Size: 1935 4888 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6823 XmaI C'CCGG_G Cuts at: 1870 4148 1870 Size: 2278 4545 XmnI GAAnn'nnTTC Cuts at: 4952 4952 Size: 6823 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4631 276 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI